Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense     

Wei-Na  Wang An-Li  Wang Yue  Wang Junxia  Wang Ru-Yong  Sun 《Journal of the World Aquaculture Society》2005,36(1):1-7

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.  相似文献   

Enzyme-linked immunosorbent assay for screening the plasma residues of tetracycline antibiotics in pigs.   总被引：6，自引：0，他引：6  

H J Lee  M H Lee  P D Ryu  H Lee  M H Cho 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(5):553-556

The recommended therapeutic doses of three kinds of tetracyclines, oxytetracycline (OTC, withdrawal period, 10 days), chlortetracycline (CTC, withdrawal period, 5 days) and tetracycline (TC, withdrawal period, 5 days), were each administered to a group of 15 pigs. Blood was sampled before drug administration and during the withdrawal period. The concentration of tetracyclines in plasma, determined by semi-quantitative ELISA, was compared with that of internal standard (10 ppb as oxytetracycline). The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine the tissue residues in pigs. All 45 plasma samples from nontreated pigs showed negative in the residue of any of three tetracycline antibiotics. OTC was detected in plasma of pigs treated until the 8th day, CTC until the 4th day, and TC was detected until the 3rd day of its withdrawal period. The present study showed that the semi-quantitative ELISA easily be adopted in predicting tissue residues for tetracycline antibiotics in live pigs.  相似文献   

表面技术在内燃机气缸套中的新进展     

李梅广孙宏飞  赵剑波孔红杰 《农业装备与车辆工程》2005,(3):41-44

论述了气缸套的失效机理，针对这一缺点，较全面地阐述了当前表面技术在气缸套上的应用现状，并提出了未来气缸套的发展方向。为同行提供了参考和启迪，也为拓宽热喷涂技术的应用指明了一个方向。  相似文献   

从空育131的试种成功看寒地粳稻种质资源的创新     

孙淑红 《勤云标准版测试》2005,(2)

由于空育131熟期适宜、稳产、丰产、优质、耐寒、抗倒等特点,近几年在黑龙江省种植面积处于领先地位。但随着种植年限的增加,稻瘟病有加重的趋势,应对其正确评价。针对寒地水稻育种缓慢的主要原因———种质资源的贫乏落后,提出了资源创新两种主要途径———自已创新及外引优异种质资源。  相似文献   

如何加强市场经济条件下工程造价管理     

王正贵  孙倩  何军 《森林工程》2001,17(1):36-37

本文根据目前工程造价管理的实践，指出了存在的问题，并提出了如何加强市场经济条件下建设工程造价管理的四点具体意见，很有现实意义和指导意义。  相似文献   

谈高层建筑疏散楼梯设计中的常见错误     

孙宝玉 《林业科技情报》2001,33(4):22-23

本文对高层建筑疏散楼梯在设计中的一些错误提出了自己的观点及解决办法;阐述了疏散楼梯在设计中的重要性,必须引起设计者的足够重视。  相似文献   

推求合理畦宽的方法及应用     

孙雪峰 《中国农村水利水电》1993,(1)

为了寻求合理的节水省时畦宽,以更好地利用现有水源,在1990、1991年试验和实践的基础上,用统计回归分析方法对大城县农田畦田规格进行了分析,求出了合理畦宽,并从灌水持续时间、用电量、提水量等方面对合理畦宽的节水、节能效果进行了分析。  相似文献   

山楂缺铁黄叶病及应用硝黄铁防治研究     

郝保春  高林森  贾云云  李茂昌  孙丽敏  杨琇  张明生 《果树学报》1992,(2)

根据调查初步总结出山楂缺铁黄叶病的发生发展规律、致病因素及其对山楂生产的危害。用新研制的高效价廉、储运方便的硝黄铁矫治,效果明显。黄化病株复绿率40%～87%,三级病株减少36%～100%,叶绿素含量提高64%～224%,喷肥区比对照区株产提高25%。同时增加了果实中糖、酸、果胶及钙、铁含量,提高了果实品质。  相似文献   

萘乙酸对大白菜钙素吸收运转及防治干烧心病的研究   总被引：10，自引：1，他引：9  

闻凤英  孙德岭 《园艺学报》1991,18(2):148-152

本研究从补钙入手,探索防治干烧心病的方法。用0.7％CaCl_2·2H_2O对和50ppmNAA混合液在大白菜结球期叶面喷洒5次,经8年试验,对大白菜干烧心病的相对防治效果达80％以上。 在此基础上,采用~(45)Ca示踪试验的方法对防病机理做了进一步研究。结果表明,无论从根部还是从叶部加萘乙酸对根部和叶部~(45)Ca的吸收和运转均具有促进作用,并且改变了其在大白菜植株内的分配规律。对照株单位干叶重dpm值自外向内呈下降趋势(130×10~3─60×10~3);用NAA处理株则相反,自外向内呈增加趋势(120×10~3─250×10~3),说明萘乙酸具有促进钙素向叶球中内部运转的功效。这种效果在贮藏期间仍能保持。另外,~(45)Ca向直接受到NAA处理部位的运转量有明显增高的趋势。这一结果为补钙防治干烧心病提供了理论依据。  相似文献   

Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells.     

H K Pradhan  G C Mohanty  W Y Lee  B Patnaik 《Veterinary immunology and immunopathology》1991,29(3-4):229-238

Antibody directed against Marek's disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek's disease virus-infected chickens. Feather follicular Marek's disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.  相似文献