Presence of 2-furoylmethyl derivatives in hydrolysates of processed tomato products     

Sanz ML  del Castillo MD  Corzo N  Olano A 《Journal of agricultural and food chemistry》2000,48(2):468-471

Acid hydrolysis of Amadori compounds yields the corresponding 2-furoylmethylamino acids (2-FM-AA) that can be analyzed by ion-pair HPLC. The relative proportions of the different 2-FM-AA present in the hydrolysates of tomato products were determined to assess their usefulness as indicators of quality. In the lyophilized tomato samples stored at 50 degrees C and a(w) = 0.44 the formation of 2-FM derivatives of alanine, gamma-aminobutyric acid (GABA), asparagine, aspartic acid, glutamic acid, lysine, serine, and threonine was detected. In commercial tomato products the most abundant 2-FM-AA was 2-FM-GABA (from traces to 26.4 mg/100 g of dry matter) followed by 2-FM-lysine (furosine). Differences in 2-FM-AA contents among samples may be related to processing and storage conditions.  相似文献   

In vitro investigation into the potential prebiotic activity of honey oligosaccharides     

Sanz ML  Polemis N  Morales V  Corzo N  Drakoularakou A  Gibson GR  Rastall RA 《Journal of agricultural and food chemistry》2005,53(8):2914-2921

The effect of honey oligosaccharides on the growth of fecal bacteria was studied using an in vitro fermentation system. Prior to treatment, glucose and fructose (31.73 and 21.41 g/100 g of product, respectively) present in honey, which would be digested in the upper gut, were removed to avoid any influence on bacterial populations in the fermentations. Nanofiltration, yeast (Saccharomyces cerevisiae) treatment, and adsorption onto activated charcoal were used to remove monosaccharides. Prebiotic (microbial fermentation) activities of the three honey oligosaccharide fractions and the honey sample were studied and compared with fructooligosaccharide (FOS), using 1% (w/v) fecal bacteria in an in vitro fermentation system (10 mg of carbohydrate, 1.0 mL of basal medium). A prebiotic index (PI) was calculated for each carbohydrate source. Honey oligosaccharides seem to present potential prebiotic activity (PI values between 3.38 and 4.24), increasing the populations of bifidobacteria and lactobacilli, although not to the levels of FOS (PI of 6.89).  相似文献   

日本血吸虫非叉型尾蚴感染动物试验   总被引：2，自引：1，他引：1  

谢银杰  吴定宇  杨建发  孙绍美  孙秀涛  段纲  邹丰才 《中国畜牧兽医》2011,38(3):226-227

试验采集云南巍山地区自然条件下的钉螺,压碎钉螺、检获尾蚴,发现为非叉型尾蚴。将非叉型尾蚴400~850条/只经皮肤感染家兔,同时,用云南洱源的叉型尾蚴作对照试验。感染后50～55 d解剖兔子,试验组检获到日本血吸虫,荷虫量为10～15条/只。试验结果表明,云南巍山非叉型尾蚴能感染动物,该试验结果为丰富日本血吸虫的生活史提供了极有价值的补充。  相似文献   

生态型农业观光园规划设计   总被引：1，自引：0，他引：1  

赵志刚  谢小立  陈安磊  王凯荣 《北方园艺》2011,(11):199-202

通过阐述园区规划设计原则及理论根据,结合实例分析了生态型农业观光园的设计内容,有利于更好的研究与开发农业观光园项目。  相似文献   

Detection of two minor phosphorylation sites for bovine κ-casein macropeptide by reversed-phase liquid chromatography-tandem mass spectrometry     

Hernández-Hernández O  Lebrón-Aguilar R  Quintanilla-López JE  Sanz ML  Moreno FJ 《Journal of agricultural and food chemistry》2011,59(20):10848-10853

This work addresses the characterization of phosphopeptides in bovine κ-casein macropeptide by reversed-phase liquid chromatography-electrospray ionization-tandem mass spectrometry (RPLC-ESI-MS(2)). Two different mass spectrometers, equipped with an ion trap (IT) or a quadrupole time-of-flight (Q-TOF) analyzer, were used to perform an accurate phosphorylation site assignment. A total of 8 phosphopeptides from 26 identified peptides were characterized. MS(2) spectra of phosphopeptides were dominated by the neutral loss of a phosphoric acid molecule (H(3)PO(4)) and sufficient informative fragment ions resulting from peptide backbone cleavages enabling the elucidation of the phosphopeptide sequence. A higher number of sequence informative b and y ions were detected using a Q-TOF instead of an IT analyzer. In addition to the well-established phosphorylation sites at Ser(149) and Ser(127), this study also revealed the presence of two minor phosphorylation sites at Thr(145) and Ser(166). These findings indicate that RPLC-ESI-MS(2) on a Q-TOF analyzer is a useful technique for identifying low-abundance phosphorylation sites in caseins.  相似文献   

Determination of free inositols and other low molecular weight carbohydrates in vegetables     

Hernández-Hernández O  Ruiz-Aceituno L  Sanz ML  Martínez-Castro I 《Journal of agricultural and food chemistry》2011,59(6):2451-2455

Different low molecular weight carbohydrates including saccharides, polyalcohols, sugar acids, and glycosides have been identified and quantified in different edible vegetables from Asteraceae, Amarantaceae, Amarylidaceae, Brassicaceae, Dioscoreaceae, and Solanaceae families by gas chromatography-mass spectrometry. Apart from glucose, fructose, and sucrose, other saccharides such as sedoheptulose in chicory, spinach, cabbage, purple yam, eggplant, radish, and oak leaf lettuce, rutinose in eggplant skin, and a glycosyl-inositol in spinach have been identified. chiro-Inositol was found in all vegetables of the Asteraceae family (3.1-32.6 mg 100 g(-1)), whereas scyllo-inositol was detected in those of purple yam, eggplant, artichoke, chicory, escarole, and endive (traces-23.2 mg 100 g(-1)). α-Galactosides, kestose, glucaric acid, and glycosyl-glycerols were also identified and quantified in some of the analyzed vegetables. Considering the bioactivity of most of these compounds, mainly chicory leaves, artichokes, lettuces, and purple yam could constitute beneficial sources for human health.  相似文献   

乌冈栎地理分布与水热环境因子的关系     

谢春平  方彦  方炎明 《水土保持研究》2011,18(1):125-131

在广泛收集乌冈栎地理分布资料的基础上,利用目前常用的植被与气候相互关系的研究方法,结合温暖指数(WI)、寒冷指数(CI)、干燥度指数(K)、水热综合指数(S)、年均生物温度(BT)、可蒸散量(PET)和年均可能蒸发量率(PER),以及单一的气象因子一月均温(TM-Jan)、七月均温(TM-Jul)、最高温(TMax)、最低温(TMin)、年均温(TM-A)、年均降水量(PM)等,对乌冈栎在东亚地区的地理分布及其与气候的关系进行了研究。结果表明:乌冈栎在中国地理分布在23°-34°N、100°-120°E,日本分布在26°-37°N、128°-140°E,均属于亚热带分布。气候环境指数研究的结果表明乌冈栎各项指数平均值均较低,与亚热带分布的青冈的指数接近,因此其性质为亚热带耐寒树种。通过热量曲线的比较,反映了中日两国乌冈栎分布范围的差异。结合主成分分析可知,影响乌冈栎分布的最主要因素是温度,并将其分布区划分为5类,包括:日本区(A)、中国华东区(B)中国西南部区(C)、中国西北部区(D)和中国南部区(E)。最后建立了乌冈栎生物地理模型,对预测该物种的分布提供了科学参考依据。  相似文献   

Enhanced tumor formation in mice heterozygous for Blm mutation   总被引：1，自引：0，他引：1  

Goss KH  Risinger MA  Kordich JJ  Sanz MM  Straughen JE  Slovek LE  Capobianco AJ  German J  Boivin GP  Groden J 《Science (New York, N.Y.)》2002,297(5589):2051-2053

Persons with the autosomal recessive disorder Bloom syndrome are predisposed to cancers of many types due to loss-of-function mutations in the BLM gene, which encodes a recQ-like helicase. Here we show that mice heterozygous for a targeted null mutation of Blm, the murine homolog of BLM, develop lymphoma earlier than wild-type littermates in response to challenge with murine leukemia virus and develop twice the number of intestinal tumors when crossed with mice carrying a mutation in the Apc tumor suppressor. These observations indicate that Blm is a modifier of tumor formation in the mouse and that Blm haploinsufficiency is associated with tumor predisposition, a finding with important implications for cancer risk in humans.  相似文献   

Plant analysis interpretation based on a new index: Deviation from optimum percentage (DOP)     

Luis Montañés  Luis Heras  Javier Abadía  Manuel Sanz 《Journal of plant nutrition》2013,36(7):1289-1308

The objective of the present work is to introduce a new index (DOP: Deviation from Optimum Percentage), as an alternative methodology for plant mineral analysis interpretation. The DOP index is calculated for each of the analyzed elements by applying the following general formula: DOP = [(C x 100yCref] ‐ 100, where C is the nutrient concentration in the sample to assess and Cref is the optimal nutrient concentration used as a reference value. The calculations used to obtain the DOP index can be easily implemented in a microcomputer. An optimum nutritional situation for any element is defined by a DOP index equal to zero. The DOP index permits to know the nutrient limitation order in a given sample, giving information similar to that obtained with the DRIS method. Furthermore, the DOP indexes indicate wether a element is in defect (negative indexes) or excess (positive indexes). For comparison, the DOP index has been applied, together with other traditional methods including the DRIS approach, to a new nutrient database from a peach tree study and to other available databases from the scientific literature. Other examples are shown indicating the different behavior of DRIS and DOP method when nutrient contents change.  相似文献   

Flower analysis as a new approach to diagnosing the nutritional status of the peach tree     

Manuel Sanz  Luis Montañés 《Journal of plant nutrition》2013,36(8):1667-1675

The chemical diagnosis of the nutritional status of peach (Prunus persica L. Batsch) trees by leaf analysis is done too late to allow any deficiencies to be corrected, especially with early bearing varieties. The use of flower analysis would be it possible to detect and treat any deficiencies at an earlier stage. Based on the relationships found between nutrient concentration of flowers at full bloom and in leaves taken 60 and 120 days after full bloom (DAFB), we have established the following reference values for the interpretation of floral analyses of this crop: N = 2.91±0.27; P = 0.39±0.05; K = 1.70±0.26; Ca = 0.87±0.16; and Mg = 0.23±0.02 expressed as a percentage of the dry matter weight of flowers.  相似文献