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41.
Peng WANG Kousaku SOUMA Yuki KOBAYASHI Kei IWABUCHI Chihiro SATO Takayoshi MASUKO 《Animal Science Journal》2010,81(4):487-493
This study examined the differences between non‐inoculated (control) corn and Northern Leaf Blight (NLB)‐damaged corn (inoculated corn); dry matter (DM) yield, silage fermentation quality, nutritive value and feed intake by sheep were compared. Leaf, stem and grain dry weights and gross yield of inoculated corn were significantly (P < 0.05) decreased compared with control corn. The contents of water‐soluble carbohydrate and nitrogen‐free extract (NFE) were decreased in inoculated corn compared with control corn. Silage made from both inoculated and control corn showed good fermentation quality. The digestibility of DM, organic matter, ether extract, NFE, and energy of silage made from inoculated corn were significantly (P < 0.05) lower, and contents of total digestible nutrients (TDN) and digestible energy (DE) were also significantly (P < 0.05) lower compared with silage made from the control corn. DM intake showed no significant discrepancy between the two types of silage. TDN and DE intakes from inoculated silage were significantly (P < 0.05) lower compared with control silage. From the above results it was shown that NLB caused a decrease in DM yield and NFE content in corn and a decrease in the nutritive value and feed intake of silage. 相似文献
42.
Yamato MIZOBE Saori KURINO Yoshiaki SATA Hironori MORI Mitsutoshi YOSHIDA Kazuchika MIYOSHI 《Animal Science Journal》2010,81(4):453-460
Whether high osmolarity of a culture medium at the early culture stage affects the development of pig oocytes and miniature pig somatic cell nuclear transfer (SCNT) embryos activated by ultrasound was examined. When oocytes were cultured in modified porcine zygote medium‐3 (mPZM‐3) with increased NaCl to 138 mmol/L (mPZM‐3+NaCl; 326 mOsm) or 50 mmol/L sucrose (mPZM‐3+sucrose; 318 mOsm) for the first 2 days and then cultured in normal mPZM‐3 (273 mOsm) for 5 days, the cleavage and blastocyst formation rates were significantly (P < 0.05) higher than those of oocytes cultured in mPZM‐3 for 7 days. The cleavage and blastocyst formation rates of SCNT embryos cultured in mPZM‐3+NaCl for the first 2 days and then cultured in mPZM‐3 for 5 days were also significantly (P < 0.05) higher than those of embryos cultured in mPZM‐3 for 7 days. These results showed that the high osmolarity of a culture medium induced by increasing NaCl concentration during the first 2 days improves the development of pig oocytes and miniature pig SCNT embryos activated by ultrasound. 相似文献
43.
Shimokawa Miyama T Umeki S Baba K Sada K Hiraoka H Endo Y Inokuma H Hisasue M Okuda M Mizuno T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1389-1393
A 4-year-old, intact male Shiba dog was referred to Yamaguchi University Animal Medical Center, Yamaguchi, Japan, for the following complaints: anorexia, lethargy, intermittent fever, gingival bleeding and abdominal purpura. The dog presented with persistent neutropenia. Histopathological examination of a bone marrow sample revealed round to oval structures that resembled Hepatozoon micromerozoites and formed a "wheel-spoke" pattern. Furthermore, mature neutrophils were observed around these structures. PCR and sequencing using bone marrow aspirate confirmed Hepatozoon canis (H. canis) infection. These findings suggest that the neutropenia observed in this case was associated with osteomyelitis due to H. canis infection. This is the first report of neutropenia associated with H. canis infection. H. canis infection can be included in the differential diagnosis in canine cases of neutropenia in areas where the disease is endemic. 相似文献
44.
Suzuki J Sasaoka F Fujihara M Watanabe Y Tasaki T Oda S Kobayashi S Sato R Nagai K Harasawa R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1113-1115
We examined the presence of hemoplasmas, hemotropic mycoplasmas, among 11 sheep (Ovis aries) with regenerative and hemolytic anemia and found six of them were positive by real-time PCR. The positive samples were then subjected to conventional PCR for direct sequencing of the 16S rRNA gene. Nucleotide sequences of all the positive samples were identified as the 16S rRNA gene of `Candidatus Mycoplasma haemovis' by phylogenetic analysis, demonstrating the infections with this particular hemoplasma species in Japan. 相似文献
45.
Umeki S Suzuki R Shimojima M Ema Y Yanase T Iwata H Okuda M Mizuno T 《Veterinary immunology and immunopathology》2011,142(1-2):119-125
Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system. 相似文献
46.
日本奶牛的饲养主要依赖于国外饲料的进口,国际饲料价格的上涨使得日本奶业陷入了危机,奶农的生产经营状况直接关系到奶业产业的发展。本研究基于日本社团法人中央畜产会实施的全国农户生产经营指导项目中所调查的294个奶农农户的生产经营相关数据,采用因子分析、多元线性回归分析、聚类分析方法,对影响奶农经营收入的因素进行了分析。结果表明,奶牛饲养的收益、奶牛饲养的劳动效率、饲养规模这三个因素对奶农的生产影响最大;阐明了奶农生产中存在的问题;提出了奶农生产经营的改善应该着重于适度扩大生产规模;合理进行投资,并且均衡大型机器设备和小农器具投资的比例以改善奶农饲养效率以及饲料生产效率,提高奶农的收入。最后,借鉴日本奶业生产经营中存在的问题,总结了对我国奶业发展的启示。 相似文献
47.
Mio KOBAYASHI Toshinori YOSHIDA Risako YAMASHITA Ryo ICHIKAWA Junta NAKAHARA Kazuki NAKAMURA Hiromu OKANO Yasunori TAKAHASHI Nanao ITO Makoto SHIBUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):994
We encountered a case of cutaneous squamous cell carcinoma (SCC) in a 17-year-old female koala at a zoo. A fragile, papillary, elevated mass was found on the third digit of the right hind limb. SCC was identified histopathologically: squamous cell-like polygonal tumor cells showed a nest-like growth pattern with epidermal down growth, central keratinization and necrotic foci, and invaded dermal connective tissues. Metastatic lesions were observed in various organs, including the lung and axillary lymph node: in the lung, multiple metastatic foci similar to the primary lesion, and in the axillary lymph node, individual polygonal tumor cells infiltrated the sinusoids. Immunohistochemistry revealed that the tumor cells were positive for proliferating cell nuclear antigen, which exhibited 32–33% of labeling indices in the tumor cells. To our knowledge, this is the first report of a case of SCC in a digit of a koala. 相似文献
48.
Teruo SANO Tetsuya KOBAYASHI Akira ISHIGURO Yoshie MOTOMURA 《Journal of General Plant Pathology》2000,66(1):68-70
Grapevine yellow speckle viroid 1 (GYSVd 1) was first detected in Japan from commercial grapevines. The viroid seems to be
spreading in Japanese commercial vineyards but without causing noticeable disease symptoms. Two Japanese GYSVd 1 isolates
from the cultivars Campbell and Steuben were sequenced. The Campbell isolate is included in the type 2 variant of GYSVd 1,
and the Steuben isolate is included in the type 3. Both of the isolates were grouped into the yellow speckle-inducing variants
in the nucleotide sequence ; however, the etiological importance of this viroid on the viticulture of Japan has not yet been
determined.
Received 14 June 1999/ Accepted in revised form 25 August 1999 相似文献
49.
Shohei KOBAYASHI Natsuko KAWANO Kenji MIYADO Ryo OHTA Takahiro AKIMOTO Taichi HATAKEYAMA Maiko KAWAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):153
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is widely used as a flame retardant and is known to exhibit anti-androgenic effects in vitro and in vivo. To assess the reproductive toxicity potency of TDCIPP, we investigated the effects of 7 days of TDCIPP oral administration on epididymal sperm motion and concentration in adult male Wistar–Imamichi rats. Thirty-five days after the final administration, sperm parameters were evaluated by computer-assisted sperm analysis. Results showed that sperm swimming progression and vigor and sperm concentration in TDCIPP-treated rats were unexpectedly higher than those in control rats. TDCIPP did not significantly affect the percentage of motile sperms or sperm swimming pattern. These results contribute to the understanding of the biological effects of TDCIPP. 相似文献
50.
Sex change in coral reef fish 总被引:1,自引:0,他引:1
Masaru Nakamura Yasuhisa Kobayashi Saori Miura Mohamad Ashraful Alam Ramji Kumar Bhandari 《Fish physiology and biochemistry》2005,31(2-3):117-122
Gonadal differentiation can take many forms in fish, ranging from gonochorism, where individuals directly develop as male
or female and finally possess only testis or ovaries at sexual maturation, to hermaphroditism where the same individuals can
produce mature male and female gametes at some time in their lives. Hermaphrodite fish are, thus, an excellent model for studying
the plasticity of sex determination and differentiation in vertebrates. We have shown that sex steroids play a principal role
in sex differentiation and sex change in fish. Our laboratory implements several fish models that undergo sex change from
female to male or male to female or in both directions. In this review, we will briefly discuss recent advances in our understanding
of the mechanism of sex change in coral reef fish. 相似文献