An X chromosome gene, WTX, is commonly inactivated in Wilms tumor     

Rivera MN  Kim WJ  Wells J  Driscoll DR  Brannigan BW  Han M  Kim JC  Feinberg AP  Gerald WL  Vargas SO  Chin L  Iafrate AJ  Bell DW  Haber DA 《Science (New York, N.Y.)》2007,315(5812):642-645

Wilms tumor is a pediatric kidney cancer associated with inactivation of the WT1 tumor-suppressor gene in 5 to 10% of cases. Using a high-resolution screen for DNA copy-number alterations in Wilms tumor, we identified somatic deletions targeting a previously uncharacterized gene on the X chromosome. This gene, which we call WTX, is inactivated in approximately one-third of Wilms tumors (15 of 51 tumors). Tumors with mutations in WTX lack WT1 mutations, and both genes share a restricted temporal and spatial expression pattern in normal renal precursors. In contrast to biallelic inactivation of autosomal tumor-suppressor genes, WTX is inactivated by a monoallelic "single-hit" event targeting the single X chromosome in tumors from males and the active X chromosome in tumors from females.  相似文献   

Antioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula     

Costa LS  Fidelis GP  Telles CB  Dantas-Santos N  Camara RB  Cordeiro SL  Costa MS  Almeida-Lima J  Melo-Silveira RF  Oliveira RM  Albuquerque IR  Andrade GP  Rocha HA 《Marine drugs》2011,9(6):952-966

Fucan is a term used to denominate a type of polysaccharide which contains substantial percentages of l-fucose and sulfate ester groups. We obtained five heterofucans from Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. These heterofucans are composed mainly of fucose, glucose, glucuronic acid, galactose and sulfate. These fucans did not show anticoagulant activity in PT and aPTT tests. Their antioxidant activity was evaluated using the follow tests; total antioxidant capacity, scavenging hydroxyl and superoxide radicals, reducing power and ferrous ion [Fe(II)] chelating. All heterofucans displayed considerable activity, especially SF-1.0v which showed the most significant antioxidant potential with 90.7 ascorbic acid equivalents in a total antioxidant capacity test and similar activity when compared with vitamin C in a reducing power assay. The fucan antiproliferative activity was performed with HeLa, PC3 and HepG2 cells using MTT test. In all tested conditions the heterofucans exhibited a dose-dependent effect. The strongest inhibition was observed in HeLa cells, where SF-1.0 and SF-1.5 exhibited considerable activity with an IC50 value of 15.69 and 13.83 μM, respectively. These results clearly indicate the beneficial effect of S. filipendula polysaccharides as antiproliferative and antioxidant. Further purification steps and additional studies on structural features as well as in vivo experiments are needed to test the viability of their use as therapeutic agents.  相似文献   

Latency-Associated Transcript-Derived MicroRNAs in Herpes Simplex Virus Type 1 Target SMAD3 and SMAD4 in TGF-β/Smad Signaling Pathway     

Zahra Shojaei Jeshvaghani  Ehsan Arefian  Sara Asgharpour  Masoud Soleimani 《Iranian Biomedical Journal》2021,25(3):169

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Fifty years after deep-ploughing: Effects on yield,roots, nutrient stocks and soil structure     

Dymphie J. Burger  Florian Schneider  Sara L. Bauke  Timo Kautz  Axel Don  Wulf Amelung 《European Journal of Soil Science》2023,74(6):e13426

Deep-ploughing far beyond the common depth of 30 cm was used more than 50 years ago in Northern Germany with the aim to break root-restricting layers and thereby improve access to subsoil water and nutrient resources. We hypothesized that effects of this earlier intervention on soil properties and yields prevailed after 50 years. Hence, we sampled two sandy soils and one silty soil (Cambisols and a Luvisol) of which half of the field had been deep-ploughed 50 years ago (soils then re-classified as Treposols). The adjacent other half was not deep-ploughed and thus served as the control. At all the three sites, both deep-ploughed and control parts were then conventionally managed over the last 50 years. We assessed yields during the dry year 2019 and additionally in 2020, and rooting intensity at the year of sampling (2019), as well as changes in soil structure, carbon and nutrient stocks in that year. We found that deep-ploughing improved yields in the dry spell of 2019 at the sandy sites, which was supported by a more general pattern of higher NDVI indices in deep-ploughed parts for the period from 2016 to 2021 across varying weather conditions. Subsoil stocks of soil organic carbon and total plant-available phosphorus were enhanced by 21%–199% in the different sites. Root biomass in the subsoil was reduced due to deep-ploughing at the silty site and was increased or unaffected at the sandy sites. Overall, the effects of deep-ploughing were site-specific, with reduced bulk density in the buried topsoil stripes in the subsoil of the sandy sites, but with elevated subsoil density in the silty site. Hence, even 50 years after deep-ploughing, changes in soil properties are still detectable, although effect size differed among sites.  相似文献   

Renal pseudoaneurysm as a cause of hematuria in a colt     

Sara Larsdotter  Charles Ley  John Pringle 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(7):759-762

An 8-month-old Arabian warmblood cross colt was evaluated for overt hematuria. The horse was severely anemic on presentation. A unilateral intrarenal vascular anomaly with unilateral pyelonephritis was diagnosed. Nephrectomy was attempted but was unsuccessful, and postmortem examination demonstrated the presence of an intrarenal pseudoaneurysm.  相似文献   

Effect of tryptophana as dietary supplement on dogs with abnormal-repetitive behaviors     

Patricia Kaulfuss  Sara Hintze  Hanno Würbel 《Journal of Veterinary Behavior: Clinical Applications and Research》2009,4(2):97

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Cider proteins and foam characteristics: a contribution to their characterization     

Blanco-Gomis D  Mangas-Alonso JJ  Junco-Corujedo S  Gutiérrez-Alvarez MD 《Journal of agricultural and food chemistry》2007,55(7):2526-2531

A capillary sieving electrophoretic method for protein analysis and molecular weight determination was used to characterize ciders from Asturias, northern Spain. The total protein content (Bradford method) and the foam parameters (Bikerman method) were also analyzed to complete this characterization. The polypeptide profile, based on the molecular weight, together with exploratory and classification techniques, that is, principal component analysis (PCA) or linear discriminant analysis (LDA), allowed ciders to be differentiated on the basis of their foam assessment and the apple juice extraction technology used in the cidermaking process. In addition, the application of correlation analysis, that is, canonical correlations (CCA) or partial least-squares (PLS), revealed that the proteins with higher molecular weight were more relevant with respect to cider foamability. PLS analysis also provided a mathematical equation that is able to predict the stabilization time of foam from the polypeptide profile of the cider, because this is the foam parameter most influenced by these compounds.  相似文献   

Standardization of canine meningioma grading: Validation of new guidelines for reproducible histopathologic criteria     

Sara Belluco  Giuseppe Marano  Thibaut Lurier  Giancarlo Avallone  Chiara Brachelente  Stefano Di Palma  Roberta Rasotto  Kerstin Baiker  Andreas Beineke  Anna Oevermann  Frauke Seehusen  Fabiano José Ferreira de Sant'Ana  Patrizia Boracchi  Martí Pumarola  Maria Teresa Mandara 《Veterinary and comparative oncology》2023,21(4):685-699

Canine meningiomas are currently graded using the human grading system. Recently published guidelines have adapted the human grading system for use in dogs. The goal of this study was to validate the new guidelines for canine meningiomas. To evaluate the inter-observer agreement, 5 veterinary surgical pathologists graded 158 canine meningiomas following the human grading system alone or with the new guidelines. The inter-observer agreement for histologic grade and each of the grading criteria (mitotic grade, invasion, spontaneous necrosis, macronucleoli, small cells, hypercellularity, pattern loss and anaplasia) was evaluated using the Fleiss kappa index. The diagnostic accuracy (sensitivity and specificity) was assessed by comparing the diagnoses obtained with the 2 grading systems with a consensus grade (considered the reference classification). The consensus histologic grade was obtained by agreement between 4 experienced veterinary neuropathologists following the guidelines. Compared with the human grading alone, the canine-specific guidelines increased the inter-observer agreement for: histologic grade (κ = 0.52); invasion (κ = 0.67); necrosis (κ = 0.62); small cells (κ = 0.36); pattern loss (κ = 0.49) and anaplasia (κ = 0.55). Mitotic grade agreement remained substantial (κ = 0.63). The guidelines improved the sensitivity in identifying grade 1 (95.6%) and the specificity in identifying grade 2 (96.2%) meningiomas. In conclusion, the new grading guidelines for canine meningiomas are associated with an overall improvement in the inter-observer agreement and higher diagnostic accuracy in diagnosing grade 1 and grade 2 meningiomas.  相似文献