排序方式： 共有98条查询结果，搜索用时 31 毫秒
21.
Different factors affect developmental competence and cryotolerance in in vitro produced bovine embryo 总被引：1，自引：0，他引：1
Imai K Matoba S Dochi O Shimohira I 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(10):887-891
The present study was conducted to examine the effects of culture systems and culture media on developmental competence and freezability of bovine embryos obtained by in vitro culture of in vitro matured and fertilized (IVM-IVF) oocytes. No significant difference was observed in the proportions of oocytes developed to blastocysts, the speed at which the oocytes reached the blastocyst stage and the number of cells, when the IVM-IVF oocytes were cultured in CR1aa with or without cumulus cells. Nevertheless, more of the IVM-IVF oocytes cultured either with or without cumulus cells in CR1aa were seen to reach the blastocyst stage much sooner than those cultured with cumulus cells in TCM199 (P<0.05). The proportion of embryos developed to the blastocyst stage by day 7 in CR1aa culture was significantly higher than embryos cultured in TCM199. Viability after frozen-thawed blastocysts were obtained in vitro, was seen in a significantly higher percentage of embryos cultured in TCM199 and developed to the hatched blastocysts than in those cultured in CR1aa (P<0.05). These results indicate that CR1aa was superior to TCM199 for the potential developmental of IVM-IVF oocytes to blastocysts during in vitro culture regardless of co-culture with or without cumulus cells. But the freezability of blastocysts developed in CR1aa was inferior to those developed in TCM199. 相似文献
22.
Ayumi SUZUKI Satoko KUSUMOTO Hiromichi HORIE Yuichi TAKIKAWA 《Journal of General Plant Pathology》2002,68(4):398-400
A bacterial leaf spot disease was observed on Hedera helix (English ivy) and H. canariensis (Algerian ivy) in Japan. The causal agent was identified as Xanthomonas campestris pv. hederae (Arnaud 1920) Dye 1978.
Received 13 May 2002/ Accepted in revised form 3 July 2002 相似文献
23.
Miura H Kotani S Kohiruimaki M Ohtsuka H Kikuchi M Ohnami Y 《The Journal of reproduction and development》2008,54(3):214-216
The purpose of this study was to determine the relationships between conception rate and other parameters before estrus synchronization with a Controlled Internal Drug Release Device (CIDR) and estradiol benzoate (EB). In the estrus synchronization program, animals were injected with 2 mg EB and then received a CIDR. Seven days later, the CIDR was removed and the animals were given an injection of Prostaglandin F(2alpha). Twenty-four hours later, they received an injection of 1 mg EB, and they were artificially inseminated 24 h after that. This program was applied to 258 Holstein cows in Tohoku-machi (Aomori, Japan). Blood was collected at the beginning of the program, and the conception rate was determined about 40 days after insemination. The relationships among conception rate, blood biochemical values, age, body condition score and days in milk were statistically analyzed to determine better conditions for cow conception. The conception rate of the cows in the high progesterone group (more than 1 ng/ml, P(4)+) was significantly higher than that of the low progesterone group (less than 1 ng/ml, P(4)-; 47.9% vs. 28.6% P<0.01). In the P(4)- groups, the serum phospholipid level was significantly higher in the conception group than in the non-conception group, and the same tendency was seen in the P(4)+ groups. Blood urea nitrogen (BUN), albumin (Alb), and total cholesterol (TChol) were significantly higher in the conception group compared with the non-conception group, but no with P(4) was observed. We concluded that 1) the conception rate of the P(4)- group was remarkably low, that 2) the low conception rate and low P(4) level was related to a low PL level and that 3) BUN, Alb and TChol were higher in the conception group, although no relation with P(4) was found. 相似文献
24.
Ochiai M Miyamoto K Kaneaki T Hayashi S Nakanishi W 《Science (New York, N.Y.)》2011,332(6028):448-451
Amination of alkanes has generally required metal catalysts and/or high temperatures. Here we report that simple exposure of a broad range of alkanes to N-triflylimino-λ(3)-bromane 1 at ambient temperature results in C-H insertion of the nitrogen functionality to afford triflyl-substituted amines in moderate to high yields. Marked selectivity for tertiary over secondary C-H bonds was observed; primary (methyl) C-H bonds were inert. Addition of hexafluoroisopropanol to inhibit decomposition of 1 dramatically improved the C-H amination efficiencies. Second-order kinetics, activation parameters (negative activation entropy), deuterium isotope effects, and theoretical calculations suggest a concerted asynchronous bimolecular transition state for the metal-free C-H amination event. 相似文献
25.
Miyuki?NittaEmail author Ju?Kyong?Lee Churl?Whan?Kang Masumi?Katsuta Satoko?Yasumoto Dajun?Liu Tsukasa?Nagamine Ohmi?Ohnishi 《Genetic Resources and Crop Evolution》2005,52(7):797-804
Perilla (Lamiaceae) contains one tetraploid species, P. frutescens (L.) Britt. and three diploid species, P. citriodora (Makino) Nakai, P. hirtella Nakai and P. setoyensis G. Honda. Tetraploid species have been traditionally cultivated in Asia for their seed oil and for their fragrant leaves
that are used as medicine or as a garnish for fish. The center of diversity is still obscure. To conserve the genetic resources,
it is important to know the diversity of the tetraploid species. The three diploid species, which are possible parents of
the tetraploid species, are all believed to be indigenous to Japan. Their distribution in China and Korea was clarified on
the basis of herbarium and field surveys. The tetraploid species is assumed to have originated somewhere around the mid-to
downstream area of the Changjiang River. Though Perilla is not cultivated as often in these areas as in northern China, Korea, the Himalayan region, or Myanmar, these areas should
also be important for the conservation of genetic resources of tetraploid Perilla crops because of the expected high genetic diversity. 相似文献
26.
This study was conducted to improve the developmental ability of nuclear transfer (NT) embryos by using blastomeres from in vitro fertilized (IVF) embryos with high quality as donor cells. The IVF embryos selected at the 2-cell stage at 24-h postinsemination (hpi) and again at the ≥8-cell stage at 48 hpi (Selected-IVF-embryos) showed the highest blastocyst formation rate among embryos. When blastomeres from the Selected-IVF-embryos (Selected-NT group) or Nonselected-IVF-embryos (Non-selected-NT group) were used as donor cells for NT, the blastocyst formation rate in the Selected-NT group (25.6%) was significantly higher than that in the Non-selected-NT group (13.5%). When blastomeres from the Selected-IVF-embryos at 108 (contained many cells before cell division) and 126 hpi (contained many cells immediately after cell division) were used as donor cells for NT (108- and 126-NT groups, respectively), the 126-NT group showed a significantly higher blastocyst formation rate (32.1%) than the 108-NT group (16.8%). Embryo transfer of blastocysts in the 126-NT group showed that 11 of 23 recipients became pregnant; nine calves were obtained. For the NT embryos reconstructed using in vivo derived embryos, 9 of 20 recipients became pregnant; seven calves were obtained. These results indicate that the blastocyst formation rate of NT embryos can be improved by using blastomeres from IVF embryos selected at the early developmental stage, especially immediately after cell division, and that the resultant NT embryos have a high developmental ability to progress to term that is comparable to NT embryos reconstructed using in vivo derived embryos. 相似文献
27.
28.
Ayaka OKADA Satoko IZUME Kenji OHYA Hideto FUKUSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1293-1297
VP22 is a major tegument protein of Equine herpesvirus type 1 (EHV-1) that is a conserved
protein among alphaherpesviruses. However, the roles of VP22 differ among each virus, and
the roles of EHV-1 VP22 are still unclear. Here, we constructed an EHV-1 VP22 deletion
mutant and a revertant virus to clarify the role of VP22. We found that EHV-1 VP22 was
required for efficient viral growth in cultured cells, but not for virulence in a hamster
model. 相似文献
29.
Narita M Young AR Arakawa S Samarajiwa SA Nakashima T Yoshida S Hong S Berry LS Reichelt S Ferreira M Tavaré S Inoki K Shimizu S Narita M 《Science (New York, N.Y.)》2011,332(6032):966-970
Protein synthesis and autophagic degradation are regulated in an opposite manner by mammalian target of rapamycin (mTOR), whereas under certain conditions it would be beneficial if they occurred in unison to handle rapid protein turnover. We observed a distinct cellular compartment at the trans side of the Golgi apparatus, the TOR-autophagy spatial coupling compartment (TASCC), where (auto)lysosomes and mTOR accumulated during Ras-induced senescence. mTOR recruitment to the TASCC was amino acid- and Rag guanosine triphosphatase-dependent, and disruption of mTOR localization to the TASCC suppressed interleukin-6/8 synthesis. TASCC formation was observed during macrophage differentiation and in glomerular podocytes; both displayed increased protein secretion. The spatial coupling of cells' catabolic and anabolic machinery could augment their respective functions and facilitate the mass synthesis of secretory proteins. 相似文献
30.
Kanazawa K Hashimoto T Yoshida S Sungwon P Fukuda S 《Journal of agricultural and food chemistry》2012,60(17):4359-4368
It is desirable to increase the flavonoid contents of postharvest vegetables since flavonoids play a beneficial role in human health promotion. In the present study, we show that postharvest vegetables increasingly produced flavonoids when irradiated with light near the absorption wavelength of flavonoids in the plant. Three-day exposure to UV-B for 5 min, 98 μmol m?2 s?1 per day, increased the contents of jaceidin in spinach, kaempherol glycoside in radish sprout, apigenin glycosides in parsley, and isovitexin in Indian spinach after 6 days of storage in a refrigerator, compared to the contents in plants without irradiation. Six days of storage of unripe green strawberry under green light for 5 min, 98 μmol m?2 s?1 per day, enabled them to mature and turn red, accompanied by 3.5-fold increased contents of pelargonidin. Elucidation of the mechanism in parsley found the stimulating expression of the flavonoid synthesis gene, PAL, C4H, 4CL, CHS, and FNS, 6 h after exposure to single irradiation with UV-B for 5 min, and the higher expression was maintained for 24 h. After 3 days irradiation during 6 days of storage, parsley did not show adverse changes in the contents of ascorbic acid, β-carotene, chlorophyll, and moisture. 相似文献