Immunolocalization of angiogenic growth factors in the ovine uterus during the oestrus cycle and in response to Steroids          下载免费PDF全文

TD Tremaine  AA Fouladi‐Nashta 《Reproduction in domestic animals》2018,53(3):667-679

The vascular changes associated with endometrial maturation in preparation for embryo implantation depend on numerous growth factors, known to regulate key angiogenic events. Primarily, the vascular endothelial growth factor (VEGF) family promotes vascular growth, whilst the angiopoietins maintain blood vessel integrity. The aim was to analyse protein levels of VEGFA ligand and receptors, Angiopoietin‐1 and 2 (ANG1/2) and endothelial cell receptor tyrosine kinase (TIE‐2) in the ovine endometrium in the follicular and luteal phases of the oestrus cycle and in response to ovarian steroids. VEGFA and its receptors were localized in both vascular cells and non‐vascular epithelium (glandular and luminal epithelium) and stroma cells. VEGFA and VEGFR2 proteins were elevated in vascular cells in follicular phase endometrium, compared to luteal phase, most significantly in response to oestradiol. VEGFR1 was expressed by epithelial cells and endothelial cells and was stimulated in response to oestradiol. In contrast, Ang‐1 and Ang‐2 proteins were elevated in luteal phase endometrium compared to follicular phase, and in response to progesterone, evident in vascular smooth muscle cells and glands which surround TIE‐2‐expressing blood vessels. Our findings indicate that VEGFA is stimulated by oestradiol, most predominantly in follicular phase endometrium, and Ang‐1 and 2 are stimulated by progesterone and were increased during the luteal phase of the oestrus cycle, during the time of vascular maturation.  相似文献   

Antimicrobial resistance in beef and dairy cattle production     

Call DR  Davis MA  Sawant AA 《Animal health research reviews / Conference of Research Workers in Animal Diseases》2008,9(2):159-167

Observational studies of cattle production systems usually find that cattle from conventional dairies harbor a higher prevalence of antimicrobial resistant (AMR) enteric bacteria compared to organic dairies or beef-cow operations; given that dairies usually use more antimicrobials, this result is not unexpected. Experimental studies have usually verified that application of antimicrobials leads to at least a transient expansion of AMR bacterial populations in treated cattle. Nevertheless, on dairy farms the majority of antibiotics are used to treat mastitis and yet AMR remains relatively low in mastitis pathogens. Other studies have shown no correlation between antimicrobial use and prevalence of AMR bacteria including documented cases where the prevalence of AMR bacteria is non-responsive to antimicrobial applications or remains relatively high in the absence of antimicrobial use or any other obvious selective pressures. Thus, there are multi-factorial events and pressures that influence AMR bacterial populations in cattle production systems. We introduce a heuristic model that illustrates how repeated antimicrobial selection pressure can increase the probability of genetic linkage between AMR genes and niche- or growth-specific fitness traits. This linkage allows persistence of AMR bacteria at the herd level because subpopulations of AMR bacteria are able to reside long-term within the host animals even in the absence of antimicrobial selection pressure. This model highlights the need for multiple approaches to manage herd health so that the total amount of antimicrobials is limited in a manner that meets animal welfare and public health needs while reducing costs for producers and consumers over the long-term.  相似文献   

Nutritional evaluation of wheat straw treated with Crinipellis sp. in Sahiwal calves     

Munnurpal Satyanarayana Mahesh  Madhu Mohini  Pankaj Jha  Sanjay Pandurang Sawant  Shivlal Singh Kundu  Ramesh Chander Kuhad 《Tropical animal health and production》2013,45(8):1817-1823

Wheat straw was subjected to solid-state fermentation (SSF) with lignolytic white-rot fungus (WRF) Crinipellis sp. for 5 days to improve the nutritive value and digestibility. The fungal treatment caused a significant (P?<?0.05) decrease in cell wall constituents viz., neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose, lignin, and cellulose to the extent of 10.4, 11.2, 8.7, 8.7, and 12.1 %, respectively, with increase (P?<?0.05) in crude protein (CP) (51.6%) and ash (25.8%) contents in fungal treated wheat straw (FT-WS) than untreated wheat straw (UT-WS). Further, in vitro gas production, in vitro true dry matter digestibility and in vitro true organic matter digestibility at 48 h, metabolizable energy (ME) content, microbial biomass production, and short-chain fatty acids synthesis were significantly (P?<?0.05) higher in FT-WS. In vivo feeding trial in 10 Sahiwal calves (8–12 months) comprised of (1) control group (T1) fed with ad libitum chopped UT-WS and (2) treatment group (T2) offered with ad libitum chopped FT-WS, in addition to supplementation of groundnut cake and green berseem (Trifolium alexandrium) forage to both groups. Digestibility of nutrients for dry matter (DM), organic matter, CP, NDF, ADF, hemicellulose, cellulose, and total carbohydrates were significantly (P?<?0.05) higher in T2 compared to T1. Moreover, daily DM (P?<?0.05), digestible crude protein (P?<?0.01), and ME intakes were also higher (P?<?0.05) in group T2 with higher (P?<?0.05) nitrogen (N) retention, which resulted in significantly (P?<?0.05) higher average daily gain in body weight (135 vs. 102 g/day). It was concluded that SSF with WRF Crinipellis sp. holds potential in upgrading the nutritional worth of wheat straw for feeding growing calves.  相似文献   

Changes in the Expression of the Prolactin Receptor (PRLR) Gene in Different Physiological Stages in the Mammary Gland of the Iranian Adani Goat          下载免费PDF全文

S Morammazi  AA Masoudi  R Vaez Torshizi  A Pakdel 《Reproduction in domestic animals》2016,51(4):585-590

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Detection and isolation of Toxoplasma gondii from fresh semen of naturally infected dogs in Southern Brazil          下载免费PDF全文

MO Koch  RR Weiss  AA Cruz  VT Soccol  KA Gonçalves  MAF Bertol  OC Beltrame  RL Dittrich 《Reproduction in domestic animals》2016,51(4):550-554

The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T. gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T. gondii in fresh semen samples using in vitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T. gondii was confirmed by in vitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa (TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T. gondii DNA sequences stored in the GenBank. It has been shown that T. gondii tachyzoites may be isolated in vitro from fresh semen samples of clinically healthy dogs seropositive for T. gondii.  相似文献   

Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: terminology and application     

Stein HH  Sève B  Fuller MF  Moughan PJ  de Lange CF;Committee on Terminology to Report AA Bioavailability  Digestibility 《Journal of animal science》2007,85(1):172-180

In this review, the terminology that is used to describe the bioavailability and ileal digestibility of AA in pig feed ingredients is defined. Aspects of the methodology to establish bioavailability and ileal digestibility values also are discussed, and recommendations about the use of these values are provided. Two main factors can contribute to differences between bioavailability and ileal digestibility of AA. First, some AA, such as Lys, may be absorbed in chemical complexes that preclude their use for metabolism. Second, fermentation in the upper gut may result in a net loss or gain of AA to the animal. In addition, dietary effects on the efficiency of using bioavailable AA intake for tissue growth or milk production should be considered and may be attributed to endogenous AA losses in the hindgut and the metabolic costs associated with endogenous gut protein synthesis and losses. Ileal digestibility values may be expressed as apparent ileal digestibility (AID), standardized ileal digestibility (SID), or true ileal digestibility (TID). These terms are used to specify how ileal endogenous AA losses are reflected in digestibility values. Ileal endogenous AA losses may be separated into basal losses, which are not influenced by feed ingredient composition, and specific losses, which are induced by feed ingredient characteristics such as levels and types of fiber and antinutritional factors. Values for AID are established when total ileal outflow of AA (i.e., the sum of endogenous losses and nondigested dietary AA) is related to dietary AA intake. A concern with the use of AID values is that these are not additive in mixtures of feed ingredients. This concern may be overcome by correcting AID values for defined basal endogenous losses of AA, which yields SID values. Furthermore, if the AID values are corrected for basal and specific endogenous losses, then values for TID are calculated. However, reliable procedures to routinely measure specific endogenous losses are not yet available. It is recommended that basal ileal endogenous losses of AA should be measured in digestibility experiments using a defined protein-free diet and that these losses are reported with observed AID and SID values. It is suggested that SID values should be used for feed formulation, at least until more information on TID values becomes available.  相似文献   

Two Methods of Vitrification Followed by In Vitro Culture of the Ovine Ovary: Evaluation of the Follicular Development and Ovarian Extracellular Matrix          下载免费PDF全文

FT Bandeira  AA Carvalho  SV Castro  LF Lima  DA Viana  JSAM Evangelista  MJS Pereira  CC Campello  JR Figueiredo  APR Rodrigues 《Reproduction in domestic animals》2015,50(2):177-185

The aim of this study was to evaluate the influence of two vitrification techniques on the extra cellular matrix (ECM) and ovarian follicular development. The ovarian cortex was fragmented (9 mm³) and divided into six groups, viz. fresh control, cultured control, vitrified by the Ovarian Tissue Cryosystem (OTC) method, conventional solid surface vitrification (SSV) method, OTC/cultured and SSV/cultured. Follicles from all the fragments were analysed for morphology, development and viability. The ECM was evaluated based on the condition of collagen and reticular fibres and the immunolocalization of type I collagen and fibronectin. After 7 days of culture, the tissue vitrified by OTC revealed a higher percentage (p < 0.05) of morphologically normal (30.66%) and viable (60.00%) follicles when compared with those vitrified using the SSV technique (21.33% and 23.00%). In all the fragments cultured, regardless of the vitrification method, a significantly higher percentage of developing follicles was observed when compared with the non‐cultured tissue. Analysis of the type I collagen showed increased immunostaining after the in vitro culture in the vitrified fragments. In conclusion, the OTC is better for preserving the follicular viability and morphology and maintaining the integrity of the extracellular matrix components of the ovine ovary.  相似文献   

The elimination of injected superoxide dismutase from synovial fluid of the horse     

DE AUER  JC NG  J. HRDLICKA  AA SEAWRIGHT 《Australian veterinary journal》1989,66(4):117-119

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Pyrrolizidine alkaloidosis of cattle associated with Senecio lautu     

JW NOBLE  JdeB CROSSLEY  BD HILL†  RJ PIERCE†  RA McKENZIE‡  M. DEBRITZ§  AA MORLEY¶ 《Australian veterinary journal》1994,71(7):196-200

Summary Serious incidents of pyrrolizidine alkaloidosis of cattle in 10 herds exposed to the Australian native plant, Senecio lautus (Asteraceae), were seen in central Queensland during 1988–1992. The deaths of 226 cattle were recorded. A mean of 8% of cattle died in affected groups (range 2 to 58%). Sickness and deaths usually occurred some months after access to S lautus. Typically, affected cattle lost body condition to the point of emaciation before dying and had persistent diarrhoea. Some animals developed abnormal behaviour and died after a shorter illness. Liver specimens from affected cattle in all herds contained lesions consistent with pyrrolizidine alkaloidosis. Thin layer chromatography of extracts of blood and liver samples from cattle from 5 herds detected pyrrolic metabolites. The identity of these was confirmed by mass spectroscopy on samples from one herd. Unseasonal autumn and winter rain after a dry summer appeared to favour growth of S lautus at the expense of other pasture species. A subsequent dry period promoted consumption of S lautus and was followed by a cluster of poisoning incidents.  相似文献   

Assessment of copper and zinc status of farm horses and training thoroughbreds in south-east Queensland   总被引：2，自引：0，他引：2  

DE AUER  JC NG  AA SEAWRIGHT 《Australian veterinary journal》1988,65(10):317-320

The copper and zinc concentrations in the blood of stabled thoroughbred horses and in Australian Stock Horses mares at pasture, either late pregnant or lactating were determined by an atomic absorption spectroscopic method. The plasma concentration of the trace elements in these apparently normal horses were generally below the "normal" range. The plasma copper, caeruloplasmin copper, whole blood copper and plasma zinc concentrations in the stabled thoroughbreds were 0.76 +/- 0.19 micrograms/ml (n = 82), 0.56 +/- 0.14 micrograms/ml (n = 83), 0.75 +/- 0.18 micrograms/ml (n = 82) and 0.47 +/- 0.09 micrograms/ml (n = 83) respectively. The plasma copper and zinc concentrations of all the brood mares at pasture (pregnant and lactating) were 0.56 +/- 0.20 micrograms/ml and 0.47 +/- 0.11 micrograms/ml (n = 30). The plasma copper concentration of the pregnant group of mares (0.64 +/- 0.18 micrograms/ml; (n = 14) was greater than that of the lactating mares (0.49 +/- 0.21; (n = 16). Variation in the plasma copper concentration was also identified between stabled and farm horses, between horses of different stables and between horses of different ages. The proportion of plasma copper bound to caeruloplasmin was 73 +/- 11.8%. These low concentrations of copper and zinc in the plasma of apparently normal horses are of clinical significance since recent evidence has indicated that copper deficiency appears to promote the development of skeletal abnormalities in foals. An alternative to the use of a single plasma sample to identify the copper or zinc deficient horse was discussed.  相似文献