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Strategies for educational action to meet veterinary medicine's role in biodefense and public health
Baker J Blackwell M Buss D Eyre P Held JR Ogilvie T Pappaioanou M Sawyer L 《Journal of veterinary medical education》2003,30(2):164-172
It is clear that the profession is not well prepared to respond to society's needs in bio-defense and public health. The imperatives that face the veterinary profession, as emphasized by the agenda for action conference deliberations that are reported in this issue of the journal, require action on many fronts, but possibly none more essential than to address how veterinary education needs to change to meet these challenges. Addressing these needs, participants at the agenda for action conference met in groups of 30 to 50 to shape approaches that would address these key questions. The 161 participants were broadly representative of government, private practice, corporate practice, organized veterinary medicine, and academia (Appendix A). Reported here are the results of those deliberations, with each of the seven sections written up by the discussion leader. Included in the participants were 20 students, representative of eight different veterinary colleges, who both participated in the group discussions and have presented their own report. 相似文献
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ABSTRACT We evaluated effects of both physical and biological components of the environment on growth of Pantoea agglomerans on inoculated pear and apple blossoms and on spread of the bacterium to blossoms on non-inoculated trees. The center three rows of 0.35- to 0.5-ha blocks of four pear cultivars and four apple cultivars were sprayed with a suspension of streptomycin-resistant P. agglomerans strain C9-1S (C9-1S) at 20 to 60% and 60 to 90% bloom. Cultivars were chosen to create a sequence of continuous bloom from late March (d'Anjou pear) through mid-May (Red Rome apple). Each cultivar block was quartered into plots; two plots were treated twice with streptomycin sulfate near mid- and full bloom to suppress populations of indigenous bacterial epiphytes and the other two plots were treated with water. Colonization of blossoms by C9-1S and by indigenous bacterial epiphytes were monitored on inoculated trees and along transects of noninoculated trees. Immediately after spraying, C9-1S was detected principally on blossoms sampled from inoculated trees. As bloom progressed, trees up to 18 m from inoculated trees had high proportions of blossoms colonized by C9-1S. Streptomycin significantly (P = 0.05) reduced incidence of isolation and size of detectable populations of culturable bacteria (indigenous bacteria plus C9-1S) from pear blossoms in 1998 and from apple blossoms in both 1998 and 1999, but the antibiotic treatment did not affect incidence of isolation, size of detectable populations, or spread of C9-1S compared to the water-treated control in any experiment. Across all cultivars, relative area under the curve for size of detectable populations of C9-1S on inoculated trees and for incidence of isolation of C9-1S from noninoculated trees was positively correlated with mean degree hours per day during bloom (r= 0.61 to 0.73) and negatively correlated with the proportion of days with rain (r = -0.79 to -0.84). The results indicate that establishment and growth of C9-1S on pome fruit flowers was not strongly affected by streptomycin or by competition from indigenous bacterial epiphytes and, as with Erwinia amylovora, temperature is an important environmental variable affecting successful spread of this biological control agent from blossom to blossom. 相似文献
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Rapid detection of Escherichia coli virulence factor genes using multiplex real-time TaqMan PCR assays 总被引:1,自引:0,他引:1
West DM Sprigings KA Cassar C Wakeley PR Sawyer J Davies RH 《Veterinary microbiology》2007,122(3-4):323-331
Three multiplex real-time TaqMan PCR assays were developed for the detection of Escherichia coli virulence factor genes in veterinary samples. Target virulence factors chosen were the fimbriae K88 (F4), K99 (F5), F41, F17, F18 and 987p (F6) and the toxins LT, STa and CDT IV. Detection of genes coding GAD were included in each assay as an internal control. These assays allow rapid identification of virulence factor genes using identical cycling conditions on an Mx3000Ptrade mark real-time PCR machine with the capacity to test up to 20 strains for 9 virulence genes in 1h. 相似文献