Expression of Marek's disease virus phosphorylated polypeptide pp38 produces splice variants and enhances metabolic activity     

Li X  Jarosinski KW  Schat KA 《Veterinary microbiology》2006,117(2-4):154-168

The phosphorylated polypeptide (pp)38 of oncogenic Marek's disease (MD) herpesvirus (MDV) is expressed during lytic infections in vivo and in vitro, but its functions have not been fully elucidated. The quail cell line QT-35, latently infected with MDV, was used to generate QTP32 in which pp38 is expressed under control of a tetracycline controlled promoter to examine possible functions of pp38. Induction of pp38 did not influence late MDV genes expression, but it enhanced mitochondrial dehydrogenase activity significantly. Two new pp38 splice variants were found in induced QTP32 cells, in additional in vitro systems and MDV-infected chickens. Differential expression of full-length pp38 and splice variants suggests that the splice variants are important during latency and perhaps transformation. Polypeptides of 40 and 20kDa were detected by Western blot using monoclonal antibody H19. These polypeptides were also produced in DF-1 cells transfected with a pp38 construct in which the splice acceptor sites had been mutated. Our results add important new information to the role of pp38 in the pathogenesis of MD. The data suggest that pp38 and the two newly described splice variants may influence metabolic activity, which may have important consequences for the understanding of latency and tumor development.  相似文献   

Chicken infectious anemia virus: an example of the ultimate host-parasite relationship   总被引：1，自引：0，他引：1  

Miller MM  Schat KA 《Avian diseases》2004,48(4):734-745

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Detection of chicken anemia virus DNA in embryonal tissues and eggshell membranes   总被引：1，自引：0，他引：1  

Miller MM  Ealey KA  Oswald WB  Schat KA 《Avian diseases》2003,47(3):662-671

Chicken infectious anemia virus (CIAV) is a ubiquitous and highly resistant virus of chickens that causes anemia and death in chicks less than 3 wk of age and immunosuppression in chickens older than 3 wk of age. The production of specific-pathogen-free eggs free of CIAV is essential for research and vaccine production. Currently, flocks are screened for CIAV by antibody tests to ensure freedom from CIAV infection. Recent evidence, however, indicates that chickens may carry and vertically transmit CIAV DNA independently of their antibody status. In this study, we tested embryos and eggshell membrane residues by nested polymerase chain reaction (PCR) as a sensitive method of detecting CIAV DNA. CIAV DNA could be detected in the blastodisks and semen obtained from antibody-positive and -negative chickens. Examination of different tissues between 18 and 20 days of incubation indicated that many but not all organs of individual embryos were positive. The lymphoid organs and gonads had the highest incidence of CIAV DNA, which was significantly different (P < 0.05) from the incidence in the liver. Eggshell membrane samples from embryos or newly hatched chicks were an excellent noninvasive source for the detection of CIAV DNA, identifying significantly more positive embryos than did pooled lymphoid organs. The use of dexamethasone injections as a method to improve the detection of carrier birds did not result in an increase of vertical transmission or cause seroconversion in the treated hens. A combination of testing eggshell membrane residues at hatch and periodic testing of blood DNA by nested PCR can be used to identify chickens carrying CIAV DNA and may be used to eradicate carrier birds.  相似文献   

Comparative pathogenesis studies with oncogenic and nononcogenic Marek's disease viruses and turkey herpesvirus.     

B W Calnek  J C Carlisle  J Fabricant  K K Murthy  K A Schat 《American journal of veterinary research》1979,40(4):541-548

An apparently nononcogenic Marek's disease virus (SB-1) and turkey herpesvirus could be readily isolated from spleen, bursa of Fabricius, thymus, and peripheral blood lymphocytes of chickens beginning 4 to 6 days after inoculation, but unlike infections with two isolates of oncogenic Marek's disease virus (JM-10 and CU-2), virus replication in these cells was rare, and necrosis in the organs was essentially absent. Splenic enlargement was observed regularly during the first 4 to 11 days after inoculation, and Marek's disease tumor-associated surface antigen was observed on splenic and other lymphocytes in the four viral inoculation groups. Cellular cytotoxicity of splenic lymphocytes was demonstrated in vitro with cultured Marek's disease tumor cells (MSB-1 lymphoblastoid cell line) as the target in a chromium-release assay. The four viral infections induced sensitized lymphocytes.  相似文献   

防治免疫抑制性疾病的新策略     

Karel A.Schat  罗何峰  刘岩雯 《国外畜牧学(猪与禽)》2008,28(6)

要生产出健康的鸡群很大程度上取决于传染性疾病的控制.新疫苗技术的开发或培育出能抵抗免疫抑制性疾病的转基因鸡,在技术上已成为可行,但是这在经济上尚不能令人满意.  相似文献   

Tracing possible drivers of synchronously fluctuating species catches in individual logbook data     

M. JANKOVSKÝ  D. S. BOUKAL  K. PIVNIČKA  J. KUBEČKA 《Fisheries Management and Ecology》2011,18(4):297-306

Abstract Recreational fisheries statistics can provide valuable information on the dynamics of fish stocks and their exploitation. For some reservoirs in the Czech Republic, there are conspicuous synchronous fluctuations in catches of different species that might be caused by fishing skills and strategies. This study describes a method that could detect signatures of such phenomena in individual logbooks. It classifies anglers by species reported during 1 year and compares the resulting angler groups by group size, fishing effort, catch per unit effort (CPUE) and annual catch. The method is illustrated by data from one reservoir, showing that the number of generalist anglers who caught several species was higher than expected. Generalists also had higher catches and effort but lower CPUE than specialists who caught only one of the species. The results indicate that generalist anglers with a low degree of specialisation and high effort could contribute to long‐term correlations in species catches.  相似文献   

Fire cycle of the Canada's boreal region and its potential response to global change     

ZHANG Quan-fa  CHEN Wen-jun .Wuhan Botanical Garden  Chinese Academy of Sciences  Wuhan  P. R. China .Applications Division  Canada Centre for Remote Sensing  Booth Street  Ottawa  Ontario KA Y  Canada 《林业研究》2007,(1)

Interactions of fire cycle and plant species' reproductive characteristics could determine vegetation distribution pattern of a landscape. In Canada's boreal region, fire cycles before the Little Ice Age (c. 1850s) ranged from 30-130 years and 25-234 years afterwards until the settlement period (c. 1930s) when longer fire cycles occurred in response to climatic change and human interference. Analysis indi-cated that fire cycles were correlated with growing season (April-October) temperature and precipitation departure from the 1961-1990 nor-mal, varying by regions. Assuming that wildfires will respond to future warming similar to the manner during the past century, an assess-ment using climatic change scenarios CGCM1, CGCM2 and HadCM2 indicates fire cycles would divert to a range of 80-140 years in the west taiga shield, more than 700 years for the east boreal shield and east taiga shield, and 300-400 years for the boreal plains in 2050.  相似文献   

The persistence of foot-and-mouth disease virus on wool   总被引：1，自引：0，他引：1  

KA McCOLL  HA WESTBURY  RP KITCHING†  VM LEWIS 《Australian veterinary journal》1995,72(8):286-292

SUMMARY Five Suffolk sheep, held in a high-security isolation room, were exposed for 2 hours to the aerosol of 3 mature pigs that had been infected with foot-and-mouth disease virus (FMDV), strain O₁-BFS. The fleeces of 3 of the sheep were contaminated with FMDV at 2 days post exposure (dpe), while at 5 dpe the fleeces of all 5 sheep were more extensively, and more heavily, contaminated. The persistence of FMDV on contaminated wool was examined in vitro using multiple 0.5 g samples of Merino wool that were each contaminated with one of 3 strains of FMDV in tissue-culture medium: O₁-BFS, O-Morocco (O-MOR 9/91) or an Asia 1 strain (TAI 1/90). Wool samples were held at either 4°C, 18°C or 37°C, and decay curves were established for each virus at each temperature. These curves predicted that O₁-BFS, O-MOR 9/91 and TAI 1/90 would fall below detect-able levels at 72, 70 and 48 days post contamination (pc), respectively, for wool stored at 4°C; at 11, 12 and 12 days pc, respectively, for wool stored at 18°C; and at 57, 68 and 33 hours pc, respectively, for wool stored at 37°C. For wool contaminated with O₁-BFS-infected sheep faeces, urine or blood, or with O₁-BFS-infected cattle saliva, decay curves predicted virus to persist for 5 to 11 days pc at 18°C. We demonstrated that the simulated scouring of FMDV-contaminated wool at 60° to 70°C would usually reduce virus to below detectable levels. The detergent component of the scouring process had little, if any, antiviral activity, and scouring at 20°C or 50°C had limited impact on FMDV titres . We recommend that either (1) simple storage of FMDV-contaminated wool for 4 weeks at temperatures of 18°C or higher, or (2) scouring of contaminated wool at 60° to 70°C would be sufficient to remove the threat of FMDV-contaminated wool being infectious to other animals .  相似文献   

Interferon production by strains of herpesvirus associated with Marek's disease     

K A Schat  J Gonzalez  A Solorzano 《Avian diseases》1974,18(4):531-535

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Humoral immune responses to chicken infectious anemia virus in three strains of chickens in a closed flock   总被引：1，自引：0，他引：1  

Cardona C  Lucio B  O'Connell P  Jagne J  Schat KA 《Avian diseases》2000,44(3):661-667

This is a comparative study on seroconversion to chicken infectious anemia virus (CIAV) in a closed flock of specific-pathogen-free chickens undergoing a natural outbreak and after vaccination of some of these flocks with a commercial, live vaccine. The N2a strain (B21B21 haplotype) had the highest seroconversion after natural infection (94%) or vaccination (100%), followed by the P2a strain (B19B19) at 75%-82% seroconversion after natural infection and 85% seroconversion after vaccination. The S13 (B13B13) chickens were 26% seropositive after natural infection and 75% seropositive after vaccination. N2a chickens with polymerase chain reaction (PCR)-positive tissues were 97% seropositive compared to 80%-83% PCR-positive and seropositive for the P2a chickens and only 8% seropositive and PCR-positive for the S13 chickens. Seroconversion occurred at or near sexual maturity after natural infection in seven flocks studied.  相似文献