Graham Stutchbury ‘Stutch’     

Rick Atwell  AE Jackson 《Australian veterinary journal》2014,92(9):356-356

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In vitro Maturation of Oocytes from Santa Ines Ewes Subjected to Consecutive Sessions of Follicular Aspiration by Laparoscopy     

LC Padilha  PPM Teixeira  EA Pires‐Buttler  M Apparício  TF Motheo  PAP Savi  EYO Nakaghi  AE Alves  WRR Vicente 《Reproduction in domestic animals》2014,49(2):243-248

The success of embryo production in vitro depends upon the use of an efficient oocyte retrieval technique, and the best results have been obtained by laparoscopic aspiration. The aim of this study was to evaluate the effect of consecutive sessions of follicular aspiration on the quantity, quality and in vitro maturation competence of oocytes obtained from ewes subjected to hormonal stimulation. Six Santa Ines ewes underwent nine sessions of follicular aspiration by laparoscopy with a 7‐day interval between sessions, totalling 56 aspirations. After 24 h of culture, oocytes were stained and classified according to the stage of nuclear and cytoplasmic maturation. Oocyte retrieval rate was 61.4 ± 2%, resulting in a total of 249 oocytes. No significant variation was observed between sessions (p > 0.05). The average number of oocytes retrieved from each ewe was 6.4 ± 2 per session and 42 ± 4 in total. No significant difference was observed between the frequencies of the different stages of nuclear maturation: 32.72% mature, 40.74% immature and 26.54% degenerated/indeterminate oocytes; however, a significant difference was observed between the frequencies of the different stages of cytoplasmic maturation: 10.7% mature, 73.25% immature and 16.05% degenerated/indeterminate oocytes. No significant difference was observed in nuclear or cytoplasmic maturation between the weeks of procedure. We conclude that after nine consecutive sessions of follicular aspiration, the quantity and quality of retrieved oocytes remained unchanged as well as the levels of nuclear and cytoplasmic maturation obtained, demonstrating the viability of this technique for repetitive follicular aspirations on the same donor.  相似文献   

In this issue – November 2013     

AE Jackson 《Australian veterinary journal》2013,91(11):441-442

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In this issue – January/February 2013     

AE Jackson  N Metzl 《Australian veterinary journal》2013,91(1-2):1-3

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In this issue – October 2013     

AE Jackson 《Australian veterinary journal》2013,91(10):395-396

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Correlation between orthopaedic and radiographic examination findings and arthroscopic ligament fibre damage in dogs with cruciate ligament rupture     

AE Ashour  CL Hoffman  P Muir 《Australian veterinary journal》2019,97(12):490-498

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Hair and saliva test fails to identify allergies in dogs     

K. Coyner  A. Schick 《The Journal of small animal practice》2019,60(2):121-125

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In this issue – April 2018          下载免费PDF全文

AE Jackson 《Australian veterinary journal》2018,96(4):99-100

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In this issue – August 2018: Qualitative interviews in veterinary research · History of CT and osteomyelitis · Minimally invasive repair of humeral fractures in dogs · Body temperature recording in koalas · BVDV antibodies not found in South Australian sheep          下载免费PDF全文

AE Jackson 《Australian veterinary journal》2018,96(8):275-276

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Pre‐Selection Test to Identify High Responder Donor Goats          下载免费PDF全文

MFA Balaro  FZ Brandão  ALRS Maia  JMG Souza‐Fabjan  MI Cueto  AE Gibbons  JF Fonseca 《Reproduction in domestic animals》2016,51(3):386-391

The aim of the study was to evaluate the feasibility of pre‐selection of high or low responder does prior to the superovulatory protocols. Twenty Saanen does received 800 IU of equine chorionic gonadotropin (eCG) at the end of long‐term progestogen treatment. Fourteen days later, a second progestogen protocol associated with a multiple‐dose follicle stimulation hormone (FSH) treatment (5 IU/kg of FSH, in six decreasing doses between days 4 to 6 of the protocol) was administered. Transrectal ultrasound was used to assess the follicular status at the beginning of superovulatory treatments, at the oestrous onset and on the seventh day of the oestrous cycle for counting corpora lutea (CL). A significant lower number of CL was obtained in eCG‐treated in comparision with FSH‐treated does (p < 0.05). A quartic regression was able to explain the relationship between the number of CL in response to both treatments (r²=0.50; p < 0.05). Seventy per cent (14 of 20) of does maintained the same ovulatory response (high or low) after treatments. The Kappa (κ = 0.40; p < 0.05) and Spearman (rs = 0.39; p = 0.08) coefficients were able to show a relationship between treatments. Regarding the follicular status, there is a significant relationship between the number of small follicles (r = 0.71; r²=0.47; p < 0.01) and total follicles (r = 0.60; p < 0.01) at eCG and first FSH dose with the number of CL. Moreover, it was found a negative relationship between the presence of large follicles and the number of CL in response to eCG treatment (r = ?0.44; p < 0.05), but not from FSH (p > 0.05). In conclusion, the screening test with eCG has the potential to identify Saanen does that will better respond to the superovulatory protocol with FSH. In addition, it highlighted the importance of an ultrasound evaluation prior to the beginning of superovulatory treatments with FSH to characterize the follicular status and identify the potential donors of high ovulatory response in MOET programmes in goats.  相似文献