The effects of dietary T-2 toxin on acute herpes simplex virus type 1 infection in mice   总被引：3，自引：0，他引：3  

S C Friend  H B Schiefer  L A Babiuk 《Veterinary pathology》1983,20(6):737-760

Young male white Swiss mice were fed control diet or diet supplemented with 20 or 10 parts per million (ppm) of T-2 toxin for two or three weeks. These mice then were inoculated with herpes simplex virus type 1 (HSV-1) (9.6 x 10(6) plaque forming units) intraperitoneally. To compare the effects of T-2 toxin against a known immunosuppressive drug, cyclophosphamide was injected intraperitoneally at 150 mg/kg, 24 hours after treatment with HSV-1, into mice fed the control diet. Mice were necropsied and tissues were collected for microscopic and virologic examination. White Swiss mice which consumed a daily diet containing 20 ppm of T-2 toxin for two or three weeks were highly susceptible to HSV-1 infection and 27 of 36 (75%) died as a result of extensive hepatic and adrenal necrosis. Although HSV-1 was isolated from livers and brains of mice fed 20 ppm of T-2 toxin for two or three weeks, there was little or no inflammatory response found in the adrenals, livers, spinal cords, brains, or ganglia. The necrotizing encephalomyelitis observed in control mice was absent. High levels of dietary T-2 toxin appeared to be more immunosuppressive than cyclophosphamide because only one mouse died after treatment with HSV-1 and cyclophosphamide. Mice treated with cyclophosphamide had changes in brain, spinal cord, spleens, thymus, and bone marrow which were similar to those fed 20 ppm of T-2 toxin and infected with HSV-1, however, liver lesions were much less severe. HSV-1-infected mice on a diet with 10 ppm T-2 toxin had lesions of intermediate severity when compared with HSV-1-infected mice fed a diet with 20 ppm T-2 toxin and HSV-1-infected mice on control diets. Necrosis was less extensive in the livers and adrenals. The infrequent isolation of virus from liver and brain was consistent with the lack of intranuclear inclusion bodies and a more marked inflammatory response. Ten ppm of dietary T-2 toxin only depressed bone marrow and splenic red pulp to a mild or moderate degree. This may have enhanced the necrotizing encephalomyelitis observed in mice killed on days 6 and 8 after HSV-1 infection. Liver lesions were mild and those of the adrenals were moderate in mice fed control diet. The rare isolation of HSV-1 from the liver and brain and the findings of a moderate to severe necrotizing encephalomyelitis in these mice was consistent with an essentially functional immune system.  相似文献   

Consistency determination of meat and meat products. IV. Consistency of liver sausage at different temperatures     

E Scharner  G Schiefer  F Tadja-Domko 《Archiv fuer experimentelle veterinaermedizin》1973,27(6):861-869

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Mycoplasma infection and histopathology of experimental enzootic pneumonia in pigs     

B Schiefer 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1970,17(1):200-211

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Pathology of Bacillus cereus mastitis in dairy cows.   总被引：1，自引：1，他引：0       下载免费PDF全文

B Schiefer  K R Macdonald  G G Klavano    A A van Dreumel 《The Canadian veterinary journal. La revue veterinaire canadienne》1976,17(9):239-243

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Equine Colitis “X”, Still an Enigma?          下载免费PDF全文

H. B. Schiefer 《The Canadian veterinary journal. La revue veterinaire canadienne》1981,22(5):162-165

Colitis “X” is a sporadic diarrheal disease of horses with clinical signs of dehydration, electrolyte imbalances and “shock”-like features. Macroscopic and microscopic findings include signs of disseminated intravascular coagulation, necrosis of colonic mucosa and presence of large numbers of bacteria in the devitalized parts of the intestine. Recently published work suggests that the causative agent may be Clostridium perfringens, Type A, but the bacteria are recoverable only in the preliminary stages of the disease. Excess protein and lack of cellulose content in the diet is thought to be the trigger for the multiplication of the clostridial organisms. The pathological findings are pathognomonic, but clinically, a number of differential diagnoses have to be considered, such as intestinal accidents, salmonellosis, heavy metal intoxication and occlusive verminous arteritis.  相似文献   

Bovine Herpesvirus Infections          下载免费PDF全文

B. Schiefer 《Canadian journal of veterinary research》1979,43(4):460

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The Activity of Matrix Metalloproteinase-2 and -9 in Serum of Pregnant and Non-Pregnant Bitches     

S Schäfer-Somi  O Ali Aksoy  M Patzl  M Findik  N Erünal-Maral  HB Beceriklisoy  B Polat  S Aslan 《Reproduction in domestic animals》2005,40(1):46-50

The aim of this study was to investigate, whether the activity of matrix metalloproteinase (MMP)-2 and -9 in the serum of pregnant and non-pregnant bitches differs significantly. For this purpose, 81 blood samples were taken from pregnant bitches at days 5-13, 15-21, 24-31, 34-40 and 41-50 after mating, and 51 samples from non-pregnant animals at corresponding times. Relative enzyme activity, calculated as the percentage of serum enzyme activity on enzyme activity in a control sample, was determined with a commercially available assay after activation of serum MMPs with p-aminophenylmercuric acetate (APMA). In addition, serum oestradiol (E(2)) and progesterone (P(4)) concentrations were measured with an enzymeimmunoassay (EIA). In the pregnant bitches, at days 5-13 and 15-21 after mating, the mean activity of both MMPs was significantly higher than in non-pregnant animals (28.5% vs 24.5% and 27.7% vs 22.6%; p < 0.01). Moreover, in the pregnant bitches, significant correlations were detected between the serum enzyme activity and the serum concentrations of E(2) (-0.900; p < 0.05) and P(4) (+0.667; p < 0.05).  相似文献   

A Screening for the Occurrence of Autoreactive Antibodies in Sera of Pregnant and Non-pregnant Bitches     

S Schäfer-Somi  KR Herkner  S Neubauer  M Egerbacher  M Patzl  S Pollak  O Ali Aksoy  HB Beceriklisoy  H Kanca  M Findik  H Kalender  S Aslan 《Reproduction in domestic animals》2006,41(1):48-54

Sera of healthy pregnant (group I, n = 11) and non-pregnant (group II, n = 11) bitches were screened for autoantibodies (AAb). In both groups, blood samples were drawn every fifth day between days 5 and 55 after mating. Serum was analysed via indirect immunofluorescence (IIF) with the Canine ANA HEp-2 Screening Kit. In all animals, anticytoplasmic AAb were detected. Utilizing primate-heart substrate slides AAb against contractile proteins of the cytoplasm could be observed. The predominating fluorescence pattern in pregnant animals resembled above all desmin, which was proven via Western blot. The sera were then pre-incubated with tropomyosin, actin, vimentin, vinculin and keratin solutions, and assessed on HEp-2 slides and on human and canine fibroblasts as well. The latter substrate was used to verify whether the detected Ab were in fact AAb. Utilizing tropomyosin, revealed elimination of the cytoplasmic fluorescences on all three substrates. It is therefore assumed, that in sera of healthy dogs, AAb against contractile structure proteins of the cytoplasm are present regularly. The majority of pregnant bitches presented with higher end-point titres (EPT), than to be found in non-pregnant dogs. AAb against desmin played the key role in those patterns. In addition, sera were screened for thyroid specific AAb, namely thyroglobulin, thyroid peroxidase (TPO), T3 and T4, and for AAb against insulin by ELISA or Western blot (TPO). Only in two of the pregnant bitches a weak positive reaction (1:100) for T3-AAb was detected.  相似文献   

Experimental T-2 toxicosis in sheep.   总被引：3，自引：3，他引：0       下载免费PDF全文

S C Friend  D S Hancock  H B Schiefer    L A Babiuk 《Canadian journal of veterinary research》1983,47(3):291-297

Lambs received T-2 toxin at a rate of 0.6 or 0.3 mg/kg body weight per day in a protein reduced diet for 21 days to study the immunological and pathological effects of T-2 toxin in sheep. Blood was collected before T-2 treatment and on days 7, 14 and 21 of the trial for hematological and biochemical examination and for the separation of peripheral blood lymphocytes for the mitogen assay. Myeloid:erythroid ratios were determined from sternal bone marrow samples taken a day before T-2 treatment began, on day 12 and at death (day 22). Lambs treated with 0.6 mg/kg body weight of T-2 toxin daily were leukopenic on day 7 and lymphopenic on days 7 and 14. Also, on day 7, the mitogenic responses of these lambs to the B-cell mitogen, lipopolysaccharide, were significantly depressed and prothrombin times were prolonged. At necropsy, lymphoid atrophy of mesenteric lymph nodes and spleens was most marked in lambs treated with 0.6 mg/kg body weight of T-2 toxin per day. To the authors' knowledge, this is the first report of leukopenia, lymphopenia and lymphoid depletion in ruminants fed T-2 toxin.  相似文献   

Incidence of mycotic gastritis in calves up to 30 days of age.   总被引：3，自引：2，他引：1       下载免费PDF全文

J P Neitzke  B Schiefer 《The Canadian veterinary journal. La revue veterinaire canadienne》1974,15(5):139-143

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