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61.
Alleged Mycotoxicosis in Swine: Review of a Court Case   总被引:1,自引:0,他引:1       下载免费PDF全文
Vomition and diarrhea in feeder pigs, and signs of hyperestrogenism in sows and pregnant gilts in a large swine operation were thought to be caused by mycotoxins. Various toxicoanalytical tests performed were negative and the cause of the disease was never clearly established. On the basis of the Sale of Goods legislation, a court ruled that the company supplying the feed was responsible for the losses that occurred. The veterinary and legal aspects of the case are reviewed, and it is concluded that there is a need for reliable and readily available laboratory diagnosis of toxins in feedstuff. The importance of gathering whatever evidence is available and of conducting whatever tests are capable of being conducted, is stressed.  相似文献   
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A two-step semen-extending protocol was compared to a one-step protocol in its efficiency in inhibiting growth of Haemophilus somnus, Campylobacter fetus ssp. venerealis, Mycoplasma bovis , and Ureaplasma diversum in experimentally infected semen. In both protocols, the effect of an antibiotic mixture of 500 μg gentamycin, 100 μg tylosin, 300 μg lincomycin, and 600 μg spectinomycin (GTLS) was compared to a mixture of 500 IU penicillin, 500 IU streptomycin, 150 μg lincomycin, and 300 μg spectinomycin (PSLS). The one-step extending method was as effective as the two-step extending method. Both antibiotic mixtures were equally effective in controlling C. fetus . For H. somnus and U. diversum , the PSLS mixture was more effective than the GTLS mixture. It was striking that both antibiotic mixtures had no effect in decreasing the numbers of M. bovis .  相似文献   
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Uterine tissue from pregnant bitches was investigated by qualitative RT-PCR for the gene expression of local factors potentially important for the implantation of canine embryos. For this purpose, 10 bitches identified as being at the time of implantation or early placentation by means of ultrasonography before ovariohysterectomy (days 20–35, n = 10) provided tissues for comparison to tissue collected in a previous study and identified as early pregnant (n = 10) or non-pregnant (n = 4) by embryo flushing after ovariohysterectomy (days 10–12 after mating; Schäfer-Somi et al. 2008 ). Uterine tissue was excised from the middle of the left horn from early pregnant and non-pregnant animals, including from interplacental and placentation sites. The following genes were investigated: CD-4, -8; cyclooxygenase (COX)-1, -2; granulocyte macrophage-colony stimulating factor (GM-CSF); hepatocyte growth factor (HGF); insulin-like growth factor (IGF)-1, -2; transforming growth factor (TGF) and tumour necrosis factor (TNF)-α; interferon (IFN)-γ; interleukin (IL)-1β, -2, -4, -6, -8, -10, -12; leukaemia inhibitory factor (LIF) and leptin. Gene expression for CD-8, COX-1, TGF-β, HGF, IGF-1, IL-2, -4,-10, IFN-γ and LIF were detected in the pre-implantation uterus, and all except IL-2 and -10 were still detectable during the implantation and placentation stage. During implantation, mRNA for IGF-2 and GM-CSF were additionally detected. The dioestrous uterus differed from the pregnant uterus because of the absence of CD-8, IL-4 and IFN-γ and the expression of CD-4, TNF-α and IL-6. The results suggest that IL-4, IFN-γ, CD-8, GM-CSF and IGF-2 are regulated in a pregnancy-specific manner and that GM-CSF and IGF-2 probably have growth supporting and immune modulating functions during implantation of the canine embryo.  相似文献   
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