刺激隐核虫抑动抗原生物学信息及抗原特性分析   总被引：2，自引：0，他引：2  

柯翎  池洪树  龚晖 《福建农业科技》2014,(1):40-44

对刺激隐核虫抑动抗原基因进行生物学信息分析,利用抗原表位多肽制备了鼠源抗抑动抗原抗体,并研究其抗原特性.试验结果表明：利用表位串联多肽所制备的鼠源抗抑动抗原抗体包含有针对刺激隐核虫抑动抗原的特异性抗体,为刺激隐核虫单克隆抗体和疫苗的制备奠定了基础.  相似文献   

木材高速螺旋式玉米铣刀的设计与优化     

李先林  陈蕾  王笑玉 《林业机械与木工设备》2014,(5):13-16

使用砂光机磨削木材存在功耗大、噪声大和粉尘多等问题,因此设计了一种木材高速螺旋式玉米铣刀,其采用“以切代磨”的方式使这一问题得到解决。该玉米铣刀形似“玉米”,刀尖处直径Ф160～240mm,可实现高线速度切削,刀身均布有2～6排螺旋式阶梯槽,各螺旋槽上均布有6～10个刀片槽,可实现刀片逐刃切削;刀具采用组合结构,刀片前角20°～26°、后角9°～13°,刀具下装有可调高的碟形弹簧,以实现刀片磨损后的补偿,提高刀具材料利用率。通过分析计算发现,初步设计的玉米铣刀质量达105kg,刀身和刀片安全系数在83～402之间,材料浪费严重,因此在优化设计时刀身内部采用了中空结构以减轻质量,装有配重拉杆以调整平衡,增加刀片数量,优化后玉米铣刀质量降至43.5kg,安全系数降到33～70之间,完全满足强度要求。  相似文献   

广西香蕉种植户技术需求意愿及影响因素分析     

张佳  柯佑鹏  过建春  张玉梅 《广东农业科学》2014,41(20):182-187

以广西12个县(区)的257户香蕉种植户的实地调查资料为依据,通过对广西香蕉种植户技术需求优先序的分析,运用二元Logistic模型实证分析其技术需求的影响因素.结果表明,广西香蕉种植户最需要的4种技术依次为病虫害防治技术、高品质管理技术、施肥技术、新品种技术;而生产决策者特征、家庭因素、组织因素、风险因素、技术信息服务等也不同程度地影响着广西香蕉种植户技术的需求行为.  相似文献   

宁夏灌区不同类型农田土壤氮素累积与迁移特征     

柯英  郭鑫年  冀宏杰  张维理  赵营 《农业环境科学学报》2014,31(1):23-31

在宁夏灌区选择设施菜田(n=4)和水旱轮作大田(n=4),通过田间多点取样观测和室内分析的方法,研究了2种类型农田土壤氮素累积与分布特点,以及其迁移对浅层地下水的影响。结果表明,设施菜田0~150 cm土壤剖面溶解性总氮(TSN)、硝态氮(NO₃^--N)和溶解性有机氮(SON)含量都显著高于大田,前者分别是后者的1.5~5.6、1.5~3.4倍和1.6~9.8倍。设施菜田土壤氮素主要累积在0~5 cm和5~20 cm土层,而大田主要在40~100 cm土体。设施菜田和大田土壤溶解性总氮占全氮比例分别在5.4%~11.5%和2.2%~4.9%之间,前者的淋失风险较高。设施菜田各形态氮素累积量表现为SON>NO₃^--N>NH₄⁺-N,大田为NO₃^--N>SON>NH₄⁺-N。设施菜田浅层地下水中TSN、NO₃^--N和SON含量也都显著高于大田,前者平均含量分别是后者的9.5、13.8倍和7.0倍。因此,硝态氮和溶解性有机氮都是2种类型农田氮素累积的主要形态,也是浅层地下水污染的重要来源。  相似文献   

Insights into an undescribed high-elevation lake (6,170 m a.s.l.) on Volcán Llullaillaco: A physical and microbiological view     

Pablo Aran  Pablo Aguilar  Thomas Bowen  Matthew Farson  Joseline Tapia  Lara Vimercati  John L. Darcy  Adam J. Solon  Dorota Porazinska  Steven K. Schmidt  Cristina Dorador 《水产资源保护:海洋与淡水生态系统》2021,31(8):2293-2299

1. High elevation lakes are extreme ecosystems and serve as sentinels of various global changes.
2. An expedition to Volcán Llullaillaco in 1996 discovered an unstudied high-elevation lake (6,170 m a.s.l.) that probably was formed as a result of the past eruptive events or climatic processes such as glacial retreat in the lake basin.
3. This article describes an initial physical characterization of the lake and its microbial communities derived from two sampling expeditions in 2013 and 2016.
4. The microbial community in the lake, with an area between 1.2 and 1.4 ha and a depth of 6.8 m, was dominated by Proteobacteria, Actinobacteria and Haloarchaea. In addition, 26 bacterial isolates were identified within the genera Subtercola, Xylophilus, Rhodanobacter, Mesorhizobium and Pseudomonas.
5. Lago Llullaillaco is one of the highest recorded lakes in the world, and this study highlights the unique microbial diversity of this aquatic ecosystem and the importance of its preservation to understand the complex biological processes under polyextreme conditions.

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青贮微生物及其对青贮饲料发酵品质影响的研究进展   总被引：1，自引：0，他引：1  

许冬梅  张萍  柯文灿  郭旭生 《草地学报》2017,25(3)

青贮微生物在饲料发酵过程中起关键作用。无论是牧草表面附着的微生物还是外源添加的微生物菌剂在青贮发酵过程中对青贮微生物的群落结构及其演替过程都起着重要的作用,而青贮发酵过程中微生物的群落结构及其演替过程与代谢产物很大程度上影响着青贮饲料的发酵品质。本文阐述了青贮微生物群落结构,微生物在青贮过程中的演替及其对青贮饲料品质的影响。从目前研究结果来看,不同牧草及同种牧草生长在不同环境中其表面附着微生物存在很大差异。添加乳酸菌对提高青贮品质效果不稳定,还需了解牧草附着微生物特性决定是否需要接种乳酸菌以得到优质青贮饲料。  相似文献   

Mass Spawning by Green Algae on Coral Reefs     

KE Clifton 《Science (New York, N.Y.)》1997,275(5303):1116-1118

Predawn episodes of mass spawning by green algae (up to nine species in five genera on a single morning) intermittently cloud Caribbean waters. Species- and sex-specific bouts of anisogamous gamete release occurred synchronously and predictably on a given morning, with closely related species spawning at different times. Algal sexual reproduction was seasonal, but, unlike the mass-spawning behavior of other sessile marine organisms, showed no lunar or tidal cycling. The discovery of mass-spawning behavior by these algae has important implications for future studies of the reproductive ecology and speciation of a vital, yet poorly understood, component of the coral reef community.  相似文献   

Malignant meningioma with lung metastases in a Boxer     

P Schmidt  C Geyer  A Hafner  K Bise  W Hermanns 《Tier?rztliche Praxis》1991,19(3):315-319

The rare case of a meningioma with pulmonary metastases in a dog is described. Clinically, the ten-year-old boxer bitch showed generalized seizures, strabismus and deficient proprioception. The post-mortem examination revealed a basically localized meningeal tumor, having the light- and electron-microscopic appearance of a malignant meningotheliomatous meningioma. Immunohistochemically, the tumor cells did not show any positive reaction with antibodies to GFAP, S-100 protein, NSE, vimentin, cytokeratin, desmin, and von Willebrand factor (factor VIII related antigen). Immunohistochemical examination of seven other canine meningiomas showed an identical pattern. The results and the relevant literature are discussed.  相似文献   

混凝土早期体积变化与裂缝的成因   总被引：1，自引：0，他引：1  

柯昌君  钟金衡 《湖北农学院学报》2001,21(1):72-75

分析了混凝土早期体积的变化以及裂缝的形成与混凝土体积变化的关系。  相似文献   

Scheduled breeding of gilts after estrous synchronization with altrenogest   总被引：2，自引：0，他引：2  

D L Davis  J S Stevenson  W E Schmidt 《Journal of animal science》1985,60(3):599-602

Fertility of 104 gilts artificially inseminated (AI) at a predetermined time (scheduled AI) after estrous synchronization with altrenogest (15 mg X gilt-1 X d-1 for 18 d) was compared with that of 103 gilts checked for estrus (estrus checked) and inseminated after altrenogest. Scheduled-AI gilts were inseminated once on d 5, 6 and 7 after the last altrenogest feeding (d 0). Estrus-checked gilts were exposed to a boar twice daily at 0830 and 1630 h and inseminated after the second and third estrous detection period following first detected estrus. Percentage of gilts assigned to treatment that farrowed (72.8 vs 67.3%), total pigs farrowed (11 +/- .4 vs 11.3 +/- .4) and pigs born alive (10.1 +/- .4 vs 10.5 +/- .4) were similar for estrus-checked and scheduled-AI gilts, respectively. We conclude that scheduled AI can be used with estrous synchronization for gilts and may have advantages in breeding herd management and the use of AI in swine.  相似文献