Post-Death Cloning of Endangered Jeju Black Cattle (Korean Native Cattle): Fertility and Serum Chemistry in a Cloned Bull and Cow and Their Offspring     

Eun Young KIM  Dong Hwan SONG  Min Jee PARK  Hyo Young PARK  Seung Eun LEE  Hyun Yong CHOI  Jeremiah Jiman MOON  Young Hoon KIM  Seong Ho MUN  Chang Eon OH  Moon Suck KO  Dong Sun LEE  Key Zung RIU  Se Pill PARK 《The Journal of reproduction and development》2013,59(6):536-543

To preserve Jeju black cattle (JBC; endangered native Korean cattle), a pair of cattle, namely a post-death cloned JBC bull and cow, were produced by somatic cell nuclear transfer (SCNT) in a previous study. In the present study, we examined the in vitro fertilization and reproductive potentials of these post-death cloned animals. Sperm motility, in vitro fertilization and developmental capacity were examined in a post-death cloned bull (Heuk Oll Dolee) and an extinct nuclear donor bull (BK94-13). We assessed reproductive ability in another post-death cloned cow (Heuk Woo Sunee) using cloned sperm for artificial insemination (AI). There were no differences in sperm motility or developmental potential of in vitro fertilized embryos between the post-death cloned bull and its extinct nuclear donor bull; however, the embryo development ratio was slightly higher in the cloned sperm group than in the nuclear donor sperm group. After one attempt at AI, the post-death cloned JBC cow became pregnant, and gestation proceeded normally until day 287. From this post-death cloned sire and dam, a JBC male calf (Heuk Woo Dolee) was delivered naturally (weight, 25 kg). The genetic paternity/maternity of the cloned JBC bull and cow with regard to their offspring was confirmed using International Society for Animal Genetics standard microsatellite markers. Presently, Heuk Woo Dolee is 5 months of age and growing normally. In addition, there were no significant differences in blood chemistry among the post-death cloned JBC bull, the cow, their offspring and cattle bred by AI. This is the first report showing that a pair of cattle, namely, a post-death cloned JBC bull and cow, had normal fertility. Therefore, SCNT can be used effectively to increase the population of endangered JBC.  相似文献   

Preparation of monodisperse poly(vinyl alcohol) (PVA) nanoparticles by dispersion polymerization and heterogeneous surface saponification     

Young?Jae?Lee  Ga?Hyun?Lee  Jun?Seong?Hwang  Sang?Won?Jeong  Hyun-Chul?Kim  Eunjoo?Kim  Tae?Hwan?Oh  Sung?Jun?LeeEmail author  Se?Geun?Lee 《Fibers and Polymers》2016,17(4):502-511

Monodisperse poly(vinyl alcohol) (PVA)/poly(vinyl acetate) (PVAc) nanoparticles with a skin-core structure were prepared through heterogeneous surface saponification of PVAc nanoparticles. For the preparation of PVAc nanoparticles with a uniform particle size distribution, vinyl acetate (VAc) was dispersion polymerized in a mixed solvent of ethanol and water using PVA with a low degree of saponification as a stabilizer. Increase of the amount of ethanol in media, the resulting PVAc nanoparticle size increases due to increasing solubility of VAc and oligomer PVAc. To preserve the sphericity and size uniformity of PVAc nanoparticles, we restricted saponification to the surface of the nanoparticles by using a small amount of aqueous sodium hydroxide solution. To determine the proper concentration of alkali solution for heterogeneous saponification, monodisperse PVAc nanoparticles were saponified with different concentrations of alkali solution at 25 °C for 0.5–3.0 h. The PVA/PVAc nanoparticles obtained by the heterogeneous saponification with 4 % (relative to the amount of the VAc) alkali solution for 2.0 h were uniformly shaped and monodispersed with diameter ranging from 428 to 615 nm. Transmission electron microscopy (TEM) confirmed the spherical nature and regular skin-core structure of the PVA/PVAc nanoparticles.  相似文献   

Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats   总被引：2，自引：0，他引：2  

Santi Devi Upadhaya  Ha Guyn Sung  Chan Hee Lee  Se Young Lee  Sun Woo Kim  Kyung Jin Cho  Jong K. Ha 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(1):29-34

The aflatoxin B1 degrading abilities of two different ruminants were compared in this study. One set of experiments evaluated the aflatoxin B1 degradation ability of different rumen fluid donors (steers vs. goats) as well as the rumen fluid filtration method (cheese cloth filtered vs. 0.45 µm Millipore) in a 2 × 2 factorial arrangement. Additional studies examined aflatoxin B1 degradation by collecting rumen fluid at different times (0, 3, 6, 9 and 12 h) after feeding. Cannulated Holstein steers (740 ± 10 kg bw) and Korean native goats (26 ± 3 kg bw) were fed a 60% timothy and 40% commercial diet with free access to water. Rumen fluid from Korean native goats demonstrated higher (p < 0.01) aflatoxin B1 degradability than Holstein steers. However, filtration method had no significant influence on degradability. In addition, aflatoxin degradation did not depend upon rumen fluid collection time after feeding, as no significant differences were observed. Finally, a comparison of two types of diet high in roughage found aflatoxin degradability in goats was higher with timothy hay opposed to rice straw, although individual variation existed. Thus, our findings showed the aflatoxin degradability is comparatively higher in goats compared to steers.  相似文献   

Development of EST‐derived SSR markers in pea (Pisum sativum) and their potential utility for genetic mapping and transferability     

Raghvendra K. Mishra  Baniekal H. Gangadhar  Akula Nookaraju  Sushil Kumar  Se W. Park 《Plant Breeding》2012,131(1):118-124

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Isolation and characterization of Streptococcus sp. from diseased flounder (Paralichthys olivaceus) in Jeju Island     

Gun Wook Baeck  Ji Hyung Kim  Dennis Kaw Gomez  Se Chang Park 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(1):53-58

Streptococcus sp. is gram-positive coccus that causes streptococcal infections in fish due to intensification of aquaculture and caused significant economic losses in fish farm industry. A streptococcal infection occurred from cultured diseased olive flounder (Paralichthys olivaceus) in May, 2005 at a fish farm in Jeju Island, Korea. The diseased flounder exhibited bilateral exophthalmic eyes and rotten gills; water temperature was 16~18℃ when samples were collected. Of the 22 fish samples collected, 3 samples were identified as Lactococcus garvieae and 18 samples were identified as Streptococcus parauberis by culture-based, biochemical test. Serological methods such as slide agglutination, hemolysis and antimicrobial susceptibility test were also used as well as multiplex PCR-based method to simultaneously detect and confirm the pathogens involved in the infection. S. parauberis and L. garvieae have a target region of 700 and 1100 bp., respectively. One fish sample was not identified because of the difference in the different biochemical and serological tests and was negative in PCR assay. In the present study, it showed that S. parauberis was the dominant species that caused streptococcosis in the cultured diseased flounder.  相似文献   

油菜菌核病发生规律及防治技术研究简报   总被引：1，自引：0，他引：1  

徐金妹茆实张国林陈海新 《中国农学通报》2003,19(2):86-86

本地属长江中下游冬油菜区,常年油菜菌核病发生危害重,2001～2002年对其发生消长规律系统观测,结果表明：3月初叶片始病,当气温回升到15℃,相对湿度达80%以上时,病情激增快;5月上旬末茎病株率激增,中旬末达到高峰。花期雨水多,气温高是该病重发的主导因素,品种、播种期、密度、长势、开花期对其发生也有一定影响。防治上,在农业防治基础上,坚持化学防治,实行花期全程药控,可有效地控制油菜菌核病的发生危害。  相似文献   

Integrated nutrient management for environmental-friendly rice production in salt-affected rice paddy fields of Saemangeum reclaimed land of South Korea     

Jae Young Cho  Jae Gwon Son  Chang Hoon Song  Seon Ah Hwang  Young Min Lee  Se Young Jeong  Byung Yeoup Chung 《Paddy and Water Environment》2008,6(3):263-273

This study was conducted in an attempt to determine the proper nitrogen and phosphorus application levels, nitrogen split application ratio, and application method for environmental-friendly rice production in a salt-affected rice paddy field, which was located in the Saemangeum reclaimed tidal belt on the western coast of South Korea, between April 1, 2003 and October 10, 2004. All treatments were replicated three times in a randomized block design (5 m × 4 m plot) with 11 treatments (total 33 plots). We designed three treatments for the evaluation of reasonable application levels of nitrogen and phosphorus fertilizers (A1–A3); five treatments to evaluate the nitrogen split application system (T1–T5); and three treatments to determine the proper application for chemical fertilizer (M1–M3). There was no significant difference of amylose and protein content among the application levels, application methods, and nitrogen split application ratios (P < 0.05). No significant differences in grain yield and yield components of rice were observed among the different application levels, application methods, and nitrogen split application ratios (P < 0.05). In order to save labor in agricultural households, preserve or enhance the grain quality of rice, and reduce nutrient losses, we determined that the optimum application level of nitrogen fertilizer was 140 kg ha⁻¹; the application split ratio of nitrogen fertilizer at four different periods was 40% for basal fertilization, 20% for maximum tilling stage, 30% for the panicle formation stage, and 10% for the booting stage; and the best application methods were deep layer application and whole layer application.  相似文献   

Plasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan     

Ji Hyung Kim  Dennis K. Gomez  Toshihiro Nakai  Se Chang Park 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(1):85-87

In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm were examined to determine differences between isolates from successive outbreaks and showed different profiles by the sources of seedlings.  相似文献   

FIELD ASPECTS OF ACETONAEMIA OF DAIRY COWS.     

D. C. Blood  B.V.Se. 《Australian veterinary journal》1956,32(2):31-41

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VIBRIONIC DYSENTERY IN SWINE. The Isolation of a Vibrio from an Outbreak in new South Wales.     

D. S. Roberts  B.V.Se. 《Australian veterinary journal》1956,32(2):27-30

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