POSITIVE PRESSURE ANAESTHESIA IN SMALL ANIMALS.     

R. H. G. Burns  B.V.Se. 《Australian veterinary journal》1956,32(2):26-26

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NOTE ON DETECTION OF A HIGH LEVEL OF BLOOD OESTROGEN IN A CASE OF PREGNANCY TOXAEMIA     

G. L. McClymont  B.V.Se.  Ph.D. 《Australian veterinary journal》1955,31(6):150-151

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Post-Death Cloning of Endangered Jeju Black Cattle (Korean Native Cattle): Fertility and Serum Chemistry in a Cloned Bull and Cow and Their Offspring     

Eun Young KIM  Dong Hwan SONG  Min Jee PARK  Hyo Young PARK  Seung Eun LEE  Hyun Yong CHOI  Jeremiah Jiman MOON  Young Hoon KIM  Seong Ho MUN  Chang Eon OH  Moon Suck KO  Dong Sun LEE  Key Zung RIU  Se Pill PARK 《The Journal of reproduction and development》2013,59(6):536-543

To preserve Jeju black cattle (JBC; endangered native Korean cattle), a pair of cattle, namely a post-death cloned JBC bull and cow, were produced by somatic cell nuclear transfer (SCNT) in a previous study. In the present study, we examined the in vitro fertilization and reproductive potentials of these post-death cloned animals. Sperm motility, in vitro fertilization and developmental capacity were examined in a post-death cloned bull (Heuk Oll Dolee) and an extinct nuclear donor bull (BK94-13). We assessed reproductive ability in another post-death cloned cow (Heuk Woo Sunee) using cloned sperm for artificial insemination (AI). There were no differences in sperm motility or developmental potential of in vitro fertilized embryos between the post-death cloned bull and its extinct nuclear donor bull; however, the embryo development ratio was slightly higher in the cloned sperm group than in the nuclear donor sperm group. After one attempt at AI, the post-death cloned JBC cow became pregnant, and gestation proceeded normally until day 287. From this post-death cloned sire and dam, a JBC male calf (Heuk Woo Dolee) was delivered naturally (weight, 25 kg). The genetic paternity/maternity of the cloned JBC bull and cow with regard to their offspring was confirmed using International Society for Animal Genetics standard microsatellite markers. Presently, Heuk Woo Dolee is 5 months of age and growing normally. In addition, there were no significant differences in blood chemistry among the post-death cloned JBC bull, the cow, their offspring and cattle bred by AI. This is the first report showing that a pair of cattle, namely, a post-death cloned JBC bull and cow, had normal fertility. Therefore, SCNT can be used effectively to increase the population of endangered JBC.  相似文献   

Cellulose nanofiber-reinforced silk fibroin composite film with high transparency     

Se Youn Cho  Min Eui Lee  Youngeun Choi  Hyoung-Joon Jin 《Fibers and Polymers》2014,15(2):215-219

We successfully prepared optically transparent silk fibroin-cellulose nanofiber (CN) composite films from solvent casting using a stable CN suspension in an aqueous silk fibroin solution. The transmittance of the silk fibroin composite films was observed by a UV-visible spectrophotometer. The secondary structural change of the silk fibroin caused by the incorporation of CNs was characterized using Fourier transform infrared spectroscopy. A tensile test was carried out to investigate the mechanical properties. The results showed that the composite film exhibited visible-light transmittance of 75 %, and its mechanical strength and Young’s modulus were increased by 44 % and 35 %, respectively, as compared to a neat silk fibroin film.  相似文献   

不同类蔬菜品种营养成分含量的研究（Ⅱ）     

邵世勤  马青枝  布彩霞  郑玉桂  色莫代 《内蒙古农业大学学报(自然科学版)》1994,(3)

本文对呼和浩特市地区种植的绿叶菜类、葱蒜类、根菜类、薯芋类和瓜类等３０种蔬菜中的氨基酸、粗蛋白、租脂肪、粗纤维等成分含量进行了研究分析，对蔬菜品种选育、改善和调节人民生活膳食结构，有重要的参考价值。  相似文献   

Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats   总被引：2，自引：0，他引：2  

Santi Devi Upadhaya  Ha Guyn Sung  Chan Hee Lee  Se Young Lee  Sun Woo Kim  Kyung Jin Cho  Jong K. Ha 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(1):29-34

The aflatoxin B1 degrading abilities of two different ruminants were compared in this study. One set of experiments evaluated the aflatoxin B1 degradation ability of different rumen fluid donors (steers vs. goats) as well as the rumen fluid filtration method (cheese cloth filtered vs. 0.45 µm Millipore) in a 2 × 2 factorial arrangement. Additional studies examined aflatoxin B1 degradation by collecting rumen fluid at different times (0, 3, 6, 9 and 12 h) after feeding. Cannulated Holstein steers (740 ± 10 kg bw) and Korean native goats (26 ± 3 kg bw) were fed a 60% timothy and 40% commercial diet with free access to water. Rumen fluid from Korean native goats demonstrated higher (p < 0.01) aflatoxin B1 degradability than Holstein steers. However, filtration method had no significant influence on degradability. In addition, aflatoxin degradation did not depend upon rumen fluid collection time after feeding, as no significant differences were observed. Finally, a comparison of two types of diet high in roughage found aflatoxin degradability in goats was higher with timothy hay opposed to rice straw, although individual variation existed. Thus, our findings showed the aflatoxin degradability is comparatively higher in goats compared to steers.  相似文献