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41.
Giang Nam Pham Da Yeun Kang Min Ju Kim Se Jong Han Jun Hyuck Lee MinKyun Na 《Marine drugs》2021,19(9)
Two undescribed rearranged cadinane-type sesquiterpenoids (1–2), named sinulaketol A-B, together with one new chlorinated steroid (3), one new gorgosterol (4), one known sesquiterpene (5), one known dibromoditerpene (6) and two known polyhydroxylated steroids (7–8) were isolated from the soft coral Sinularia brassica. The structures of these compounds were established by extensive spectroscopic analysis, including HRESIMS, 1D, and 2D NMR spectroscopy. Their absolute configurations were also determined by the ECD calculations and DP4+ probability analysis. Antileishmanial activity of compounds 1–8 was evaluated in vitro against the amastigote forms of Leishmania donovani, in which compounds 3, 6, and 7 inhibited the growth of L. donovani by 58.7, 74.3, 54.7%, respectively, at a concentration of 50 μM. Antimicrobial effect of the isolated compounds were also evaluated against Candida albicans, Staphylococcus aureus, and Escherichia coli. Compound 6, a brominated diterpene, exhibited antimicrobial effect against S. aureus. 相似文献
42.
Characterization of a cinnamoyl derivative from broccoli (Brassica oleracea L. var. italica) florets
Nazneen Shaik Survay Brajesh Kumar Chandrama Prakash Upadhyaya EunYoung Ko ChoongHwan Lee Jung Nam Choi Do-Young Yoon Yi-Sook Jung Se Won Park 《Fitoterapia》2010,81(8):1062-1066
A new intact glucosinolate Cinnamoyl derivative [6'-O-trans-(4″- hydroxy cinnamoyl)-4-(methylsulphinyl) butyl glucosinolate] (A) has been isolated from Broccoli (Brassica oleracea L. var. italica) florets. The compound was isolated and characterized by LC, MS-ESI, FTIR, 1H and 13C NMR as well as 1H-1H COSY, DEPT 135° spectrometric experiments. 相似文献
43.
It is well known that inhibition of prolyl oligopeptidase (POP) is involved in memory-related function. In this study, we observed that rosmarinic acid (RA) inhibits POP activity with an IC50 of 63.7 μM. Subsequently, we investigated the cognitive-enhancing effects of RA employing the Morris water maze paradigm. The results demonstrated that RA is non-competitive POP inhibitor and that acute and subchronic RA treatments showed an inverted U-shaped dose–response curve in the platform crossings. Furthermore, chronic RA treatment significantly increased the platform crossings. These results suggest that RA has a cognitive-enhancing effect which may be mediated by inhibition of POP. 相似文献
44.
为解决西藏作物生产上易受干旱、霜冻危害寻找安全环保的新途径,选择“天达2116”对冬青稞进行拌种、叶面喷施、春天复壮、拌种+叶面喷施、拌种+春天复壮、拌种+复壮+叶面喷施等6个处理,研究其在高原这种特殊生态环境条件下对西藏冬青稞生长发育的影响。结果表明,6个处理的青稞籽粒产量均高于对照,依次比对照增产4.84%、29.49%、19.19%、14.35%、19.03%、38.07%,其中“拌种+复壮+叶面喷施”处理青稞的增产幅度最大,达38.07%,主要是通过提高青稞的千粒重、单株有效穗数来提高其产量;6个处理的青稞秸秆产量均高于对照,且增产趋势与子粒增产趋势基本一致;6个处理的青稞子粒每公顷比对照经济效益高324.30元,2047.80元,1293.30元,901.50元,1244.25元和2601.30元。因此,建议“天达2116”在西藏青稞上应用以“叶面喷施”或“拌种+复壮+叶面喷施”2个处理为最佳。 相似文献
45.
Rodríguez-Meizoso I Jaime L Santoyo S Cifuentes A García-Blairsy Reina G Señoráns FJ Ibáñez E 《Journal of agricultural and food chemistry》2008,56(10):3517-3523
In the search for new functional ingredients with potential use in the food industry, extracts of unknown species of microalgae, such as Phormidium species have been studied. Three solvents of different polarities (i.e., hexane, ethanol, and water) have been used to obtain pressurized liquid extracts with different compositions. Moreover, extractions were performed at four different extraction temperatures (50, 100, 150, and 200 degrees C) with 20 min as extraction time. Antioxidant activity of the extracts has been measured by the TEAC assay. In general, hexane and ethanol extracts showed a higher antioxidant capacity that was mainly attributed to carotenoid compounds, as the TEAC value trend seems to be similar to the carotenoid content of the extracts. On the other hand, the high antioxidant activity of the 200 degrees C water extracts is likely related to the presence of Maillard reaction compounds produced by thermal degradation of the sample. beta-Carotene, lutein, violaxanthin, and neoxanthin were identified in 150 degrees C ethanol extracts. Four different microbial species ( Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger) were used to screen the potential antimicrobial activity of the Phormidium sp. extracts. The most sensitive microorganism was the yeast, C. albicans, whereas the fungus, A. niger, was the most resistant. In general, no drastic differences were found for solvents and temperatures tested, showing a very diverse nature of the compounds responsible for the antimicrobial activity of these microalgae. In ethanol extracts, antimicrobial activity could be mainly attributed to the presence of terpenes (i.e., beta-ionone, neophytadiene) and fatty acids (i.e., palmitoleic and linoleic acids) in the samples. Toxicity studies carried out with the extracts evaluated in the present work showed a cellular toxicity lower than those of other cyanobacteria such as Spirulina plantensis. 相似文献
46.
Dennis Kaw Gomez Gun Wook Baeck Ji Hyung Kim Casiano Hermopia Choresca Se Chang Park 《Journal of veterinary diagnostic investigation》2008,20(1):38-44
Viral nervous necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. In Korea, VNN has been identified in several species of cultured marine fish. In this study, the authors present data of the amplified nested polymerase chain reaction product (420 bp) of 21 nodavirus strains from different species of apparently healthy wild marine fish on the southern coast of Korea. Phylogenetic analysis based on the partial nucleotide sequence (177 bases) of the RNA2 coat protein gene of 21 strains was highly homologous (93-100%) and closely related to that of the known betanodavirus, redspotted grouper nervous necrosis virus. These results indicate that betanodaviruses occur in large populations of wild marine fish in the southern part of the Korean peninsula, suggesting the importance of these subclinically infected fish as an inoculum source of betanodavirus that is horizontally transmitted to susceptible cultured fish species. 相似文献
47.
Na Young Yi Shin Ae Park† Man Bok Jeong‡ Won Tae Kim† Se Eun Kim† Ji Youn Kim§ Je Min Chae¶ Kyoung Jin Jang Je Kyung Seong†† Kang Moon Seo† 《Veterinary ophthalmology》2009,12(3):139-151
Objective To evaluate motility of silicone orbital implants and corneoscleral prostheses, with and without use of a motility coupling post (MCP) in dogs.
Animals studied Eighteen mixed-breed dogs.
Procedures The motility of an orbital silicone implant and corneoscleral prosthesis after enucleation ( n = 6), evisceration ( n = 6), or use of a MCP with evisceration ( n = 6) in dogs were compared. One eye from each dog had surgery whereas the opposite eye was used as a control. Clinical evaluations were performed three times a week. Histopathology of the orbital tissues was performed 8 and 12 weeks after surgery.
Results Implant motility in dogs with evisceration (vertical movement [VM] 8.04 ± 2.13; horizontal movement [HM] 11 ± 3.05) and evisceration with MCP (VM 9.61 ± 1.59); HM was significantly greater than the enucleation group (VM 0.51 ± 0.5; HM 1.22 ± 0.68) ( P < 0.01). Prosthetic motility in dogs with evisceration with MCP was significantly greater than in dogs with evisceration; dogs with evisceration had significantly greater motility than dogs with enucleation ( P < 0.01). No postoperative complications were observed in any of the groups. No significant abnormalities were noted on histopathology.
Conclusions MCP placement in silicone orbital implants significantly enhanced the prosthetic motility in dogs. This study supports the use of MCP in silicone orbital implants to enhance corneoscleral prosthesis motility and cosmetics in dogs. 相似文献
Animals studied Eighteen mixed-breed dogs.
Procedures The motility of an orbital silicone implant and corneoscleral prosthesis after enucleation ( n = 6), evisceration ( n = 6), or use of a MCP with evisceration ( n = 6) in dogs were compared. One eye from each dog had surgery whereas the opposite eye was used as a control. Clinical evaluations were performed three times a week. Histopathology of the orbital tissues was performed 8 and 12 weeks after surgery.
Results Implant motility in dogs with evisceration (vertical movement [VM] 8.04 ± 2.13; horizontal movement [HM] 11 ± 3.05) and evisceration with MCP (VM 9.61 ± 1.59); HM was significantly greater than the enucleation group (VM 0.51 ± 0.5; HM 1.22 ± 0.68) ( P < 0.01). Prosthetic motility in dogs with evisceration with MCP was significantly greater than in dogs with evisceration; dogs with evisceration had significantly greater motility than dogs with enucleation ( P < 0.01). No postoperative complications were observed in any of the groups. No significant abnormalities were noted on histopathology.
Conclusions MCP placement in silicone orbital implants significantly enhanced the prosthetic motility in dogs. This study supports the use of MCP in silicone orbital implants to enhance corneoscleral prosthesis motility and cosmetics in dogs. 相似文献
48.
Shin Ae Park Na Young Yi Man Bok Jeong Won Tae Kim Se Eun Kim Je Min Chae Kang Moon Seo 《Veterinary ophthalmology》2009,12(4):205-210
Objective To determine the incidence, etiology, and concurrent ophthalmic findings in small breed dogs that presented with cataracts.
Animal studied Five hundred and sixty-one small breed dogs (942 eyes) were presented to the Veterinary Medical Teaching Hospital of Seoul National University between July 2002 and December 2007 with cataract.
Procedure The medical records of small breed dogs with cataract were reviewed. The reason for presentation, cataract duration, bilaterality, breed, gender, age, vision, etiology, stage of cataract development, concurrent ophthalmic findings, IOP, ocular ultrasonography findings, and scotopic electroretinography (ERG) findings were investigated.
Results The most frequently presented breeds were the Miniature/Toy Poodle ( n = 112, 20.0%), Yorkshire Terrier ( n = 110, 19.6%), and Shih Tzu ( n = 95, 16.9%). The Miniature/Toy Poodle showed a significantly higher odds ratio for cataract formation (2.6). The proportion of female cataract patients was significantly higher than that of male cataract patients in the overall population ( P < 0.05). The Miniature/Toy Poodles had significantly higher numbers of females affected with cataract ( P < 0.01). The mean age for cataract formation was 8.3 ± 3.9 years. The mean age at onset in the Miniature/Toy Poodle and Yorkshire Terrier was significantly higher, whereas that in the Miniature Schnauzer group was significantly lower ( P < 0.0001). Clinical signs related to lens induced uveitis had a tendency to increase with cataract progression ( P < 0.05). There were no significant differences in the b-wave amplitude for mixed rod cone response by stage ( P = 0.137).
Conclusions Small breed dogs with cataracts had characteristics with regard to age of onset and gender distribution, depending on the breed. 相似文献
Animal studied Five hundred and sixty-one small breed dogs (942 eyes) were presented to the Veterinary Medical Teaching Hospital of Seoul National University between July 2002 and December 2007 with cataract.
Procedure The medical records of small breed dogs with cataract were reviewed. The reason for presentation, cataract duration, bilaterality, breed, gender, age, vision, etiology, stage of cataract development, concurrent ophthalmic findings, IOP, ocular ultrasonography findings, and scotopic electroretinography (ERG) findings were investigated.
Results The most frequently presented breeds were the Miniature/Toy Poodle ( n = 112, 20.0%), Yorkshire Terrier ( n = 110, 19.6%), and Shih Tzu ( n = 95, 16.9%). The Miniature/Toy Poodle showed a significantly higher odds ratio for cataract formation (2.6). The proportion of female cataract patients was significantly higher than that of male cataract patients in the overall population ( P < 0.05). The Miniature/Toy Poodles had significantly higher numbers of females affected with cataract ( P < 0.01). The mean age for cataract formation was 8.3 ± 3.9 years. The mean age at onset in the Miniature/Toy Poodle and Yorkshire Terrier was significantly higher, whereas that in the Miniature Schnauzer group was significantly lower ( P < 0.0001). Clinical signs related to lens induced uveitis had a tendency to increase with cataract progression ( P < 0.05). There were no significant differences in the b-wave amplitude for mixed rod cone response by stage ( P = 0.137).
Conclusions Small breed dogs with cataracts had characteristics with regard to age of onset and gender distribution, depending on the breed. 相似文献
49.
Simó C Ibañez E Señoráns FJ Barbas C Reglero G Cifuentes A 《Journal of agricultural and food chemistry》2002,50(23):6648-6652
Antioxidants from orange juice were determined by the combined use of countercurrent supercritical fluid extraction (CC-SFE) prior to reverse-phase liquic chromatography (RP-LC) or micellar electrokinetic chromatography (MEKC). The separation of antioxidants found in the SFE fractions was achieved by using a new MEKC method and a published LC procedure, both using diode array detection. The characterization of the different antioxidants was further done by LC-mass spectrometry. Advantages and drawbacks of LC and MEKC for analyzing the antioxidants found in the different orange extracts are discussed. Although LC yields higher peak area and slightly better reproducibility than MEKC, the latter technique provides information about the CC-SFE extracts in analysis times 7 times faster than by LC. This analysis advantage can be used for the quick adjustment of CC-SFE conditions, thus providing a fast way to obtain orange fractions of specific composition. 相似文献
50.
Ibañez E Cifuentes A Crego AL Señoráns FJ Cavero S Reglero G 《Journal of agricultural and food chemistry》2000,48(9):4060-4065
Antioxidants from rosemary were determined by the combined use of supercritical fluid extraction (SFE) prior to reverse-phase high-performance liquid chromatography (RP-HPLC) or micellar electrokinetic chromatography (MEKC). The separation of antioxidants found in the SFE fractions was achieved by using a new MEKC method and a published HPLC procedure, both with diode array detection. The characterization of the different antioxidants was further done by HPLC-mass spectrometry. Advantages and drawbacks of HPLC and MEKC for analyzing the antioxidants found in the different extracts are discussed. From the results it is concluded that HPLC renders higher peak area and is better in its reproducibility than MEKC; both techniques provide similar analysis time reproducibility. The main advantage of MEKC is its much higher separation speed, which is demonstrated to be useful for the quick adjustment of SFE conditions, allowing rosemary fractions of higher antioxidative power to be obtained quickly. Moreover, the possibilities of this approach for following the degradation of antioxidants are discussed. 相似文献