Supercritical carbon dioxide fractionation of nonesterified alkoxyglycerols obtained from shark liver oil   总被引：1，自引：0，他引：1  

Vázquez L  Fornari T  Señoráns FJ  Reglero G  Torres CF 《Journal of agricultural and food chemistry》2008,56(3):1078-1083

Ethanolysis of shark liver oil was carried out to generate a product enriched in nonesterified alkoxyglycerols and fatty acid ethyl esters. For the present study, the original oil contained very low amounts of squalene, and thus, unsaponifiable matter was mainly constituted by nonesterified alkoxyglycerols (NEAKG). A small percentage of monoesterified alkoxyglycerols (MEAKG) was also detected. Supercritical fluid extraction was employed to fractionate the mixture, achieving a complete elimination of esters and concentrating the alkoxyglycerol compounds in the raffinate product. Extractions were carried out in a countercurrent packed column, using extraction pressures in the range of 140-180 bar, temperatures from 45 to 65 degrees C, and a solvent-to-feed ratio of 15. NEAKG + MEAKG purity obtained in the raffinate at the best extraction conditions was around 78% w/w, and satisfactory yield (>60%) was also achieved. Therefore, the raffinate product can be re-esterified to design highly valuable ether lipid compounds.  相似文献   

OVINE PREGNANCY TOXAEMIA     

G. L. McClymont  B.V.Sc  Ph.D. B. P. Setchell  B.V.Se. 《Australian veterinary journal》1955,31(3):53-68

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THE VALUE OF DDT, BHC, ALDRIN AND DIELDRIN FOR THE PROTECTION OF SHEEP AGAINST BODY STRIKE     

J. H. Riches  Ph.d.  B.Agr.Sc. P. J. O'Sullivan  B.Agr.Se.† 《Australian veterinary journal》1955,31(10):258-262

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AN OUTBREAK OF SCRAPIE IN BRITISH SHEEP IMPORTED INTO VICTORIA     

L. B. Bull  F.A.A.  C.B.E.  D.V.Se. D. Murance  D.V.Se. 《Australian veterinary journal》1958,34(7):213-215

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Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates     

Dennis Kaw Gomez  Dong Joo Lim  Gun Wook Baeck  Hee Jeong Youn  Nam Shik Shin  Hwa Young Youn  Cheol Yong Hwang  Jun Hong Park  Se Chang Park 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(4):369-374

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Seroprevalence of five arboviruses in sentinel cattle as part of nationwide surveillance in South Korea, 2009?2012     

Yeon-Hee KIM  Jae-Ku OEM  Eun-Yong LEE  Koung-Ki LEE  Seong-Hee KIM  Myoung-Heon LEE  Se Chang PARK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):247-250

To investigate the possible circulation of arboviruses in South Korea, nationwide surveillance of five arbovirues was conducted in sentinel calves during 2009−2012. We used serum neutralization tests to investigate the presence of antibodies for the Aino virus, Akabane virus, bovine ephemeral fever virus, Chuzan virus and Ibaraki virus. In 2009, 2011 and 2012, the seropositive rates for these five arboviruses were all less than 14.1%. In 2010, however, the seropositive rates for Aino virus and Akabane virus were 33.2% and 40.2%, respectively. High seropositive rates were also associated with a large-scale outbreak of Akabane viral encephalomyelitis in cattle in southern Korea in 2010. Continued seroprevalence surveillance will be useful for monitoring natural arboviral diseases.  相似文献   

湘东丘陵区次生林土壤微生物的分布及酶的活性研究     

曾思齐  周国英  佘济云 《中南林业科技大学学报(自然科学版)》1998,(2)

为了探讨长江中上游低质低效次生林改造途径,摸索综合评价土壤肥力及林分土壤发育状况的方法。研究了次生林不同林分类型土壤微生物数量及酶的活性．结果表明：次生林土壤微生物数量主要集中在0～20cm的土层中,随土层加深,其数量迅速减少;不同林分类型土壤微生物数量相差很大,阔叶林、针阔混交林的数量多而针叶林的数量少;土壤酶的活性以针阔混交林、阔叶林的强．  相似文献   

Changes in some components of the muscle lipids of three freshwater fish species under natural extreme cold and temperate conditions     

Uysal K  Bülbül M  Dönmez M  Seçkin AK 《Fish physiology and biochemistry》2008,34(4):455-463

Fatty acid composition, conjugated linoleic acid and cholesterol contents in the muscles of three freshwater fish species (Barbus plebejus escherichi, Capoeta capoeta capoeta and Rutilus rutilus) were determined under natural extreme temperate (July) and cold (January) conditions. The aim of the study was to determine whether there were differences in these components of the muscle lipids among these three fish species under extreme natural conditions. Samples were analyzed using gas chromatography. Palmitic, oleic, docosahexaenoic and eicosapentaenoic acids were the predominant fatty acids in all fish in both months. The percentages of polyunsaturated fatty acids, n − 3 polyunsaturated fatty acids, n − 6 polyunsaturated fatty acids and eicosapentaenoic + docosahexaenoic acids in the muscle of B. plebejus escherichi and C. capoeta capoeta were significantly higher in January (P < 0.05) than in July. The ratio of n − 6 to n − 3 polyunsaturated fatty acids was lower than 0.60 in all fish species, with C. capoeta capoeta showing the lowest ratio in January (0.36). The levels of cholesterol and conjugated linoleic acid ranged from 103.46 to 150.10 mg/100 g oil and from 16.27 to 35.45 mg/100 g oil, respectively, for all samples in both months. There were no statistical differences in cholesterol levels among the three fish species in July and January. Conjugated linoleic acid contents were significantly higher in January in B. plebejus escherichi and C. capoeta capoeta. Of the three species tested, the extreme temperate and cold conditions affected B. plebejus escherichi the most.  相似文献   

江苏四个典型克氏原螯虾养殖区抗生素污染特征与生态风险评估   总被引：1，自引：2，他引：1  

余军楠  方昊  胡建林  王壮  丁程成  袁和忠  王瑟  崔益斌 《农业环境科学学报》2020,39(2):386-393

为揭示江苏克氏原螯虾养殖环境中抗生素的分布特征和污染现状,利用超高效液相色谱-串联质谱法(UPLC-MS/MS)调查浦口和盱眙两个地区4个典型养殖塘水和沉积物中抗生素的残留情况。共检出14种抗生素(包括4种大环内酯类、2种四环素类、4种喹诺酮类和4种磺胺类),除浦口两养殖塘水体中的CTCHCL、OFL和AZI(浓度分别为16.528~57.140ng L-1和9.803~47.400 ng L-1),其他抗生素均以低浓度存在。抗生素浓度与水质指标的相关性分析结果表明,抗生素在水环境中的降解可能受水体富营养化、有机污染程度等水质状况的影响。基于RQs值对养殖环境中的抗生素进行风险评估,它们对生态系统中浮游动物的生长基本不会造成威胁;对藻类等浮游植物而言,浦口养殖水体中部分大环内酯类(SP、CTM)和喹诺酮类(O FL)抗生素具有中高度风险(0.161≤RQs≤2.92),盱眙养殖环境中目标抗生素生态风险较低(RQs≤0.181)。研究表明,江苏(特别是盱眙)克氏原螯虾养殖水体中抗生素污染水平相对较低,但仍存在一定的生态风险。建议采取全面的抗生素控制措施,以保护水产养殖生态系统的健康。  相似文献   

A serological investigation of caseous lymphadenitis in four flocks of sheep     

Malone FE  Fee SA  Kamp EM  King DC  Baird GJ  O'Reilly KM  Murdock FE 《Irish veterinary journal》2006,59(1):19-21

A double antibody sandwich ELISA developed by ID-DLO, Lelystad to detect Corynebacterium pseudotuberculosis infection was used on 329 sheep from four pedigree Suffolk flocks in which clinical cases of caseous lymphadenitis (CLA) had occurred. At subsequent necropsy, typical CLA lesions were seen in 133 sheep, and the diagnosis was confirmed on culture. Lesions were most commonly seen in lungs (n = 46), parotid lymph nodes (n = 44), prescapular lymph nodes (n = 38) and mediastinal lymph nodes (n = 31). The sensitivity of the ELISA test for detecting culture-positive sheep was 0.88, while the specificity of the test was 0.55. The antibody ELISA detected 87.5 per cent of sheep that had CLA lesions restricted to internal organs only. It was concluded that the ELISA test has a valuable role in detecting sheep with both clinical and subclinical CLA.  相似文献