Genotypic diversity in field isolates of Babesia bovis from cattle with babesiosis after vaccination     

AE LEW  RE BOCK  JM CROFT  CM MINCHIN  TG KINGSTON  RJ DALGLIESH 《Australian veterinary journal》1997,75(8):575-578

Objective To determine whether particular genotypes of Babesia bovis were common to field isolates obtained from cattle properties in Queensland where the B bovis vaccine had apparently failed.
Design A comparative study of polymerase chain reaction genotypes in different populations of B bovis .
Procedure Two polymerase chain reaction assays were applied to analyse DNA extracts of B bovis vaccine (K, T and Dixie strains) and 27 field isolates from 24 properties where disease outbreaks had occurred despite the use of the vaccine. To evaluate the stability of the genotypes identified, 11 of the field isolates were inoculated into experimental cattle that had either been previously vaccinated with T strain or not vaccinated.
Results No particular genotype of B bovis was responsible for the problems observed in previously vaccinated herds. None of the isolates had genotypes identical to the vaccine strains used. No geographic trends among the genotypes were observed. Isolates that originated from the same property also had different genotypes. Blood passage of the 11 field isolates in either previously vaccinated or nonvaccinated cattle did not alter the original genotype.
Conclusion No particular genotypes identified by the Bv80 and BvVA1 polymerase chain reaction assays could be associated with vaccine failures.  相似文献   

Vascular hamartomas of the dorsal carpal region in three young thoroughbred horses   总被引：1，自引：0，他引：1  

CM COLBOURNE  JV YOVICH  RB RICHARDS  KJ ROSE  CR HUXTABLE 《Australian veterinary journal》1997,75(1):20-23

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Cervical diskospondylitis in two horses     

CM COLBOURNE  SL RAIDAL  JV YOVICH  JMcC HOWELL  JL RICHARDSON 《Australian veterinary journal》1997,75(7):477-479

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Congenital lymphoedema in an Ayrshire-Friesian crossbred female calf     

CM MULEI  RB ATWELL 《Australian veterinary journal》1989,66(7):227-228

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Vitrification with Glutamine Improves Maturation Rate of Vitrified / Warmed Immature Bovine Oocytes     

C Yamada  WB Feitosa  R Simões  AC Nicacio  CM Mendes  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2011,46(1):173-176

The current study examined the protective effects of l ‐glutamine and cytochalasin B during vitrification of immature bovine oocytes. Oocyte vitrification solution (PBS supplemented with 10% FCS, 25% EG, 25% DMSO and 0.5 m trehalose) was the vitrification control. Treatments were the addition of 7 μg/ml cytochalasin B, 80 mm glutamine or both cytochalasin and glutaminine for 30 s. After warming, oocytes were matured in vitro for 24 h, fixed and stained with Hoechst (33342) for nuclear maturation evaluation. l ‐glutamine improved the vitrified/warmed immature bovine oocytes viability (32.8%), increasing the nuclear maturation rates compared to other treatments and the no treatment vitrified control (17.4%). There was, however, no effect of cytochalasin B on in vitro maturation (14.4%).  相似文献   

Human IL-3 and GM-CSF act synergistically in stimulating hematopoiesis in primates   总被引：10，自引：0，他引：10  

R E Donahue  J Seehra  M Metzger  D Lefebvre  B Rock  S Carbone  D G Nathan  M Garnick  P K Sehgal  D Laston 《Science (New York, N.Y.)》1988,241(4874):1820-1823

Interleukin-3 (IL-3) is a member of a family of growth factors, each of which supports the proliferation and development of hematopoietic precursors in culture. Although the biologic effects of the different hematopoietic growth factors have been well documented in different culture systems, it has only recently become possible to study the activities of these molecules in vivo. In comparison with the later acting hematopoietic growth factors granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor, IL-3 elicited a delayed and relatively modest leukocytosis when continuously infused intravenously in primates. The IL-3 infusion, however, greatly potentiated the responsiveness of the animal to subsequent administration of a low dose of GM-CSF. These results suggest that IL-3 expands an early cell population in vivo that subsequently requires the action of a later acting factor such as GM-CSF to complete its development. Optimal stimulation of hematopoiesis may be achieved with combinations of hematopoietic growth factors.  相似文献   

Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato   总被引：6，自引：0，他引：6  

SR Scofield  CM Tobias  JP Rathjen  JH Chang  DT Lavelle  RW Michelmore  BJ Staskawicz 《Science (New York, N.Y.)》1996,274(5295):2063-2065

Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two-hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.  相似文献   

Connectivity and management of caribbean coral reefs     

CM Roberts 《Science (New York, N.Y.)》1997,278(5342):1454-1457

Surface current patterns were used to map dispersal routes of pelagic larvae from 18 coral reef sites in the Caribbean. The sites varied, both as sources and recipients of larvae, by an order of magnitude. It is likely that sites supplied copiously from "upstream" reef areas will be more resilient to recruitment overfishing, less susceptible to species loss, and less reliant on local management than places with little upstream reef. The mapping of connectivity patterns will enable the identification of beneficial management partnerships among nations and the design of networks of interdependent reserves.  相似文献   

Clinical use of atracurium in horses undergoing ophthalmic surgery     

JM  Senior  KJ Robinson  AHA Dugdale  CM Price  WA Adams  RS Jones 《Veterinary anaesthesia and analgesia》2001,28(4):207-208

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Shelf life of weaning foods developed from locally available food stuffs     

Poonam Gahlawat  Salil Sehgal 《Plant foods for human nutrition (Dordrecht, Netherlands)》1994,45(4):349-355

Four weaning foods were formulated using locally available cereals and pulses such as wheat (Triticum aestivum), barley (Hordeum vulgare), green gram (Vigna radiata) and jaggery. Cereal, pulse and jaggery were used in the proportion of 70:30:25. Roasting and malting were two processing techniques used. The developed weaning foods were evaluated for their nutritional characteristics and shelf life. All the formulations had a nutrient composition within the range prescribed by the Indian Standard Institute (ISI) for processed weaning foods. Peroxide value and fat acidity of weaning foods increased with increase in storage period. Malting of weaning foods resulted in higher increase of peroxide value and fat acidity as compared to roasted ones during the period of storage. All the blends were found to be acceptable up to 60 days of storage. The results, indicated that weaning foods developed from locally available less inexpensive foods may be used as good supplements for infants.  相似文献