Overexpression of OCT4A ortholog elevates endogenous XIST in porcine parthenogenic blastocysts     

Jae Yeon HWANG  Kwang-Hwan CHOI  Dong-Kyung LEE  Seung-Hun KIM  Eun Bae KIM  Sang-Hwan HYUN  Chang-Kyu LEE 《The Journal of reproduction and development》2015,61(6):533-540

X-chromosome inactivation (XCI) is an epigenetic process that equalizes expression of X-borne genes between male and female eutherians. This process is observed in early eutherian embryo development in a species-specific manner. Until recently, various pluripotent factors have been suggested to regulate the process of XCI by repressing XIST expression, which is the master inducer for XCI. Recent insights into the process and its regulation have been restricted in mouse species despite the evolutionary diversity of the process and molecular mechanism among the species. OCT4A is one of the represented pluripotent factors, the gate-keeper for maintaining pluripotency, and an XIST repressor. Therefore, in here, we examined the relation between OCT4A and X-linked genes in porcine preimplantation embryos. Three X-linked genes, XIST, LOC102165544, and RLIM, were selected in present study because their orthologues have been known to regulate XCI in mice. Expression levels of OCT4A were positively correlated with XIST and LOC102165544 in female blastocysts. Furthermore, overexpression of exogenous human OCT4A in cleaved parthenotes generated blastocysts with increased XIST expression levels. However, increased XIST expression was not observed when exogenous OCT4A was obtained from early blastocysts. These results suggest the possibility that OCT4A would be directly or indirectly involved in XIST expression in earlier stage porcine embryos rather than blastocysts.  相似文献   

Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development     

Seon-Ung HWANG  Yubyeol JEON  Junchul David YOON  Lian CAI  Eunhye KIM  Hyunju YOO  Kyu-Jun KIM  Kyu Mi PARK  Minghui JIN  Hyunggee KIM  Sang-Hwan HYUN 《The Journal of reproduction and development》2015,61(6):549-557

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Surveillance of classical swine fever in wild boar in South Korea from 2010–2014     

Yong Kwan KIM  Seong-In LIM  Jae-Jo KIM  Yoon-Young CHO  Jae-Young SONG  In-Soo CHO  Bang-Hun HYUN  Sung-Hyun CHOI  Seung-Hoe KIM  Eun-Hye PARK  Dong-Jun AN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1667-1671

Classical swine fever (CSF) is a highly contagious systemic hemorrhagic viral disease of pigs. Wild boar plays a crucial role in the epidemiology of CSF. Between 2010 and 2014, samples were collected nationwide from 6,654 wild boars hunted in South Korea. Anti-CSF antibodies were identified in 0.59% (39 of 6,654) of the wild boar samples using a virus neutralization test and were primarily detected in wild boars living close to the demilitarized zone and the area of the Taebaek Mountains surroundings. The CSF virus (subgroup 2.1b) was isolated from two wild boars captured in a nearby border area. The criteria used to define high-risk areas for targeted CSF surveillance in South Korea should be further expanded to include other regions nationwide.  相似文献   

Diagnosis of a large splenic tumor in a dog: computed tomography versus magnetic resonance imaging     

Mijin KIM  Sooyoung CHOI  Hojung CHOI  Youngwon LEE  Kija LEE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1685-1687

This study demonstrated magnetic resonance imaging (MRI) and computed tomography for large-sized splenic hemangiosarcoma. Radiography and ultrasonography revealed the presence of a large-sized soft-tissue mass in the cranial abdomen. Computed tomography showed hypoattenuating mass. The mass was located in contact with liver, spleen and stomach, and the origin of the mass remained ambiguous. The mass was T2-hyperintense and T1-hypointense with mild contrast enhancement. MRI allowed a differentiation between large-sized tumor and neighboring normal structure, and the mass was consequently identified as arising from spleen. These results suggested that MRI might be a useful tool to visualize large-sized splenic tumors and improve the accuracy of diagnosis.  相似文献   

论北韩革命纪念碑布局特征     

金信源  朴永吉 《山东农业大学学报(自然科学版)》2006,37(4):655-659

根据北韩的现代建筑史和时代划分,把北韩解放后革命纪念碑的形成过程划分为四个阶段,按照上述划分探讨北韩革命纪念碑的形成过程,分析了主要革命纪念碑的空间布局与功能,得出北韩革命纪念碑的一般特征。风景优美的革命纪念碑成为城市景观的一大亮点,它与城市街道在空间布局上具有相关性。革命纪念碑具有中轴对称,并与周围空间巧妙结合的空间布局特点。  相似文献   

汉江流域不同土地利用方式下土壤反硝化率及其控制因素研究     

S. P. JUNG  Y. J. KIM  H. KANG 《土壤圈》2014,24(4):516-528

Land-use patterns can affect various nutrient cycles in stream ecosystems, but little information is available about the effects of urban development on denitrification processes at the watershed scale. In the presented study, we investigated the controlling factors of denitrification rates within the streams of the Han River Basin, Korea, with different land-use patterns, in order to enhance the effectiveness of water resource management strategies. Ten watersheds were classified into three land-use patterns （forest, agriculture and urban） using satellite images and geographic information system techniques, and in-situ denitrification rates were determined using an acetylene blocking method. Additionally, sediment samples were collected from each stream to analyze denitrifier communities and abundance using molecular approaches. In-situ denitrification rates were found to be in the order of agricultural streams （289.6 mg N20-N m-2 d-1） 〉 urban streams （157.0 mg N20-N m-2 d-1） 〉 forested streams （41.9 mg N20-N m-2 d-l）. In contrast, the average quantity of denitrifying genes was the lowest in the urban streams. Genetic diversity of denitrifying genes was not affected by watershed land-use pattern, but exhibited stream-dependent pattern. More significance factors were involved in denitrification in the sites with higher denitrification rates. Multiple linear regression analysis revealed that clay, dissolved organic carbon and water contents were the main factors controlling denitrification rate in the agricultural streams, while dissolved organic carbon was the main controlling factor in the urban streams. In contrast, temperature appeared to be the main controlling factor in the forested streams.  相似文献   

大豆蛋白-多糖复合物结构与性能及其稳定性研究     

郑环宇  孔洋  郑丽  金技峰  李杨  滕飞 《农业机械学报》2022,53(15):406-415

以大豆分离蛋白（SPI）、阿拉伯树胶（GA）及卡拉胶（CA）为原料,经物理混合成功制备不同多糖与SPI复合物,考察不同添加量（SPI与GA/CA质量比为20、15、10、5）对蛋白-多糖复合物结构、性能变化趋势及其乳液稳定性的影响规律,最终探明蛋白与不同多糖复合物的相互作用机制;运用红外光谱、荧光光谱及电子显微镜解析不同复合物的结构特征,采用乳化特性、粒径、Zeta电位、表面疏水性等指标明确不同复合物的理化特性,并通过探讨不同复合物乳液的乳化活性、乳化稳定性、表观粘度及乳析指数明晰其稳定特性。结果表明,两种多糖在酸性条件下可与SPI生成复合物,并且当SPI与CA质量比为20时,复合物的Zeta电位最高,为（20.47±0.82）mV,平均粒径最小,为（1.37±0.01）μm,分布均匀,乳化活性指数最高,为（106.46±4.75）m2/g,乳化稳定性指数为（145.33±8.53）min,此时复合乳液的稳定性较好,CA的加入显著降低SPI内源荧光强度并改变SPI的二级结构,SPI与CA结合形成了稳定的复合物。  相似文献   

Glucosinolate profiles in the seeds, leaves and roots of rocket salad (Eruca sativa Mill.) and anti-oxidative activities of intact plant powder and purified 4-methoxyglucobrassicin     

Sun-Ju KIM  Gensho ISHII 《Soil Science and Plant Nutrition》2006,52(3):394-400

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Natural Amenities and Rural Development: Understanding Spatial and Distributional Attributes   总被引：1，自引：0，他引：1  

KWANG‐KOO KIM  DAVID W. MARCOUILLER  STEVEN C. DELLER 《Growth and change》2005,36(2):273-297

ABSTRACT Contemporary resource management practice and rural development planning increasingly emphasize the integration of resource extractive industries with non‐market‐based recreational and amenity values. There is a growing empirical literature which suggests that natural amenities impact regional economies through aggregate measures of economic performance such as population, income, and/or employment growth, and housing development. We maintain that assessing the developmental aspects of amenity‐led regional change requires a more thorough focus on alternative measures of economic performance such as income distribution and spatial organization. In the applied research presented here we investigate relationships between amenities and regional economic development indicators. Results suggest mixed and generally insignificant amenity‐based associations which highlight the need for appropriate regional economic modeling techniques that account for often dramatic spatial autocorrelation of natural amenity attributes. We conclude that with respect to amenity driven economic growth and development “place in space” matters.  相似文献   

Variations in Disease Resistance of Chestnut Cultivars against Chestnut Blight Fungus,Cryphonectria parasitica     

LEE Sanghyun  KIM Kyunghee  MOON Byungju 《中国林业科技(英文版)》2006,5(2):75-77

INTRODUCTION Chestnut trees cultivated worldwide are planted as fruit tree in Korea and the area is increasing annually. In the 1970s, chestnut trees were enormously planted in many provinces in Korea. However,chestnut blight occurred and increased due to lack of disease management and the aging of the trees. Blight caused by Cryphonectria parasitica is a kind of important disease and occurred in oak and maple. American chestnut is more susceptible to this pathogen than Korean or Japane…  相似文献