Effects of dietary protein and lipid level on growth and body composition of juvenile ayu (Plecoglossus altivelis) reared in seawater     

S.-M. LEE  D.-J. KIM  & S.H. CHO 《Aquaculture Nutrition》2002,8(1):53-58

A 3 (protein levels, 380, 460 and 520 g kg^–1 diet) × 2 (lipid levels, 65 and 140 g kg^–1 diet) factorial experiment with three replicates was conducted. Weight gain, feed efficiency and daily feed intake were not significantly affected by dietary protein level, but were by dietary lipid level. Weight gains of fish fed 65 g lipid kg^–1 diet were significantly, or slightly, higher than for 140 g lipid kg^–1 diet at all protein levels. Daily protein intake was significantly affected by both dietary protein and lipid levels ( P  < 0.002). Daily lipid intake was not significantly affected by dietary protein level, but was by dietary lipid level ( P  < 0.001). Protein efficiency ratio was significantly affected by dietary protein level ( P  < 0.02), but not by dietary lipid level. Protein efficiency ratio tended to improve with the decrease of dietary protein level at the same lipid level. Moisture, protein and lipid contents of whole fish were significantly affected by dietary lipid level ( P  < 0.01). Increased dietary lipid did not improve growth or feed efficiency, but increased body fat deposition. It was concluded that the optimum dietary protein and lipid level for growth of juvenile ayu may be 380 and 65 g kg^–1 diet, respectively, when fish were fed to satiety three times daily in seawater.  相似文献   

Rumen microbial response in production of CLA and methane to safflower oil in association with fish oil or/and fumarate     

Xiang Z. LI  Rui J. LONG  Chang G. YAN  Hong G. LEE  Young J. KIM  Man K. SONG 《Animal Science Journal》2011,82(3):441-450

Supplementation effect of fish oil and/or fumarate on production of conjugated linoleic acid (CLA) and methane by rumen microbes was examined when incubated with safflower oil. One hundred and twenty milligrams of safflower oil (SO), safflower oil with 24 mg fish oil (SOFO), safflower oil with 24 mmol/L fumarate (SOFA), or safflower oil with 24 mg fish oil and 24 mmol/L fumarate (SOFOFA) were added to the 90 mL culture solution. The culture solution was also made without any supplements (control). The SOFA and SOFOFA increased pH and propionate (C3) compared to other treatments from 3 h incubation time. An accumulated amount of total methane (CH₄) for 12 h incubation was decreased by all the supplements compared to control. The concentrations of c9,t11CLA for all the incubation times were increased in the treatments of SOFO, SOFA and SOFOFA compared to SO. The highest concentration of c9,t11CLA was observed from SOFOFA among all the treatments at all incubation times. Overall data indicate that supplementation of combined fumarate and/or fish oil when incubated with safflower oil could depress CH₄ generation and increase production of C₃ and CLA under the condition of current in vitro study.  相似文献   

Effects of corn dried distiller's grains with solubles and enzyme premix supplements on growth performance,carcass characteristics and meat quality parameters in finishing pigs     

Sung Dae LEE  Hyun Jung JUNG  Kyu Ho CHO  Jun Cheol PARK  In Cheul KIM  Pil Nam SEONG  Young Min SONG 《Animal Science Journal》2011,82(3):461-467

The objective of the present study was to investigate the effects of corn dried distiller's grains with solubles (DDGS) and enzyme premix (mannanase + phytase) supplementation on the growth performance, carcass and meat quality parameters in finishing pigs. Sixty hybrid pigs (L × LW × D) with initial weight of 63.92 ± 1.50 kg were used in a 3 × 2 factorial design with main effects of DDGS levels (0, 10 and 20%) and enzyme premix levels (0% vs. 0.14%). Average daily gain (ADG, P < 0.01) and average daily feed intake (ADFI, P < 0.05) were decreased due to an increased level of DDGS additive while the feed conversion ratio was improved (P < 0.05) by adding enzyme premix. The diet cost/gain (won/kg) was saved (P < 0.01) due to an increased level of DDGS additive. There were no significant differences in carcass characteristics and meat quality parameters of Longissimus dorsi muscle by DDGS level and enzyme premix. Palmitoleic acid, oleic acid and monounsaturated fatty acid (MUFA) decreased (P < 0.05) according to DDGS level. The results indicate that DDGS may be used in feeds for finishing pig as a replacement of corn and soybean meal without affecting their carcass characteristics and meat quality.  相似文献   

Comment of the Editorial Committee     

PETER W JOHNSON  JOSEPH C BORAY  KIM L DAWSON 《Australian veterinary journal》1993,70(1):37-37

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Differentiation of porcine circovirus 1 and 2 in formalin-fixed, paraffin-wax-embedded tissues from pigs with postweaning multisystemic wasting syndrome by in-situ hybridisation   总被引：2，自引：0，他引：2  

J. KIM  C. CHAE 《Research in veterinary science》2001,70(3):265-269

Non-radioactive digoxigenin (DIG)-labelled probes that can differentiate porcine circovirus (PCV) 1 from PCV2 in formalin-fixed, paraffin-wax-embedded tissues by in-situ hybridisation were developed. A 349 base pair (bp) DNA fragment from open reading frame (ORF) 1 of PCV1 and a 481 bp DNA fragment from ORF2 of PCV2 generated by polymerase chain reaction (PCR) were used as PCV1 and PCV2 probes, respectively. A specific DIG-labelled PCV1 DNA probe did not hybridise with PCV2-infected PK-15 cells and vice versa. From the 40 field cases with postweaning multisystemic wasting syndrome tested by in-situ hybridisation, 30 (75 per cent) cases were PCV2-positive only and 10 (25 per cent) cases were positive for both PCV1 and PCV2. PCV1 and PCV2 DNAS were detected mainly in the macrophages of lymph nodes and spleens. Positive cells typically exhibited a dark brown to black reaction product mainly in the cytoplasm but also occasionally in the nucleus. In-situ hybridisation together with the differential probes developed in the present study represent an additional tool capable of differentiating of both types of PCV in formalin-fixed, paraffin-wax-embedded tissues.  相似文献   

Antibody responses after vaccination against equine influenza in the Republic of Korea in 2013     

Eun-Ju KIM  Bo-Hye KIM  Sunjoo YANG  Eun-Jin CHOI  Ye-Jin SHIN  Jae-Young SONG  Yeun-Kyung SHIN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1517-1521

In this study, antibody responses after equine influenza vaccination were investigated among 1,098 horses in Korea using the hemagglutination inhibition (HI) assay. The equine influenza viruses, A/equine/South Africa/4/03 (H3N8) and A/equine/Wildeshausen/1/08 (H3N8), were used as antigens in the HI assay. The mean seropositive rates were 91.7% (geometric mean antibody levels (GMT), 56.8) and 93.6% (GMT, 105.2) for A/equine/South Africa/4/03 and A/equine/Wildeshausen/1/08, respectively. Yearlings and two-year-olds in training exhibited lower positive rates (68.1% (GMT, 14) and 61.7% (GMT, 11.9), respectively, with different antigens) than average. Horses two years old or younger may require more attention in vaccination against equine influenza according to the vaccination regime, because they could be a target of the equine influenza virus.  相似文献   

Nitrification inhibitor nitrapyrin does not affect yield-scaled nitrous oxide emissions in a tropical grassland     

Ana Gabriela P&#;REZ-CASTILLO  CristinaCHINCHILLA-SOTO  Jorge Alberto ELIZONDO-SALAZAR  RonnyBARBOZA  Dong-Gill KIM  Christoph M&#;LLER  AlbertoSANZ-COBENA  Azam BORZOUEI  Khadim DAWAR  MohammadZAMAN 《土壤圈》2021,31(2):265-278

Urea is the most common nitrogen(N)fertilizer used in the tropics but it has the risk of high gaseous nitrogen(N)losses.Use of nitrification inhibitor has been suggested as a potential mitigation measure for gaseous N losses in N fertilizer-applied fields.In a field trial on a tropical Andosol pastureland in Costa Rica,gaseous emissions of ammonia(NH₃)and nitrous oxide(N₂O)and grass yield were quantified from plots treated with urea(U;41.7 kg N ha^-1application^-1)and urea plus the nitrification inhibitor nitrapyrin(U+NI;41.7 kg N ha^-1application^-1and 350 g of nitrapyrin for each 100 kg of N applied)and control plots(without U and NI)over a six-month period(rainy season).Volatilization of NH₃(August to November)in U(7.4%±1.3%of N applied)and U+NI(8.1%±0.9%of N applied)were not significantly different(P>0.05).Emissions of N₂O in U and U+NI from June to November were significantly different(P<0.05)only in October,when N₂O emission in U+NI was higher than that in U.Yield and crude protein production of grass were significantly higher(P<0.05)in U and U+NI than in the control plots,but they were not significantly different between U and U+NI.There was no significant difference in yield-scaled N₂O emission between U(0.31±0.10 g N kg^-1dry matter)and U+NI(0.47±0.10 g N kg^-1dry matter).The results suggest that nitrapyrin is not a viable mitigation option for gaseous N losses under typical N fertilizer application practices of pasturelands at the study site.  相似文献   

茶树农杆菌转化系统和基因枪转化系统的优化   总被引：9，自引：3，他引：9  

吴姗  梁月荣  陆建良  金惠淑  吴颖 《茶叶科学》2003,23(1):6-10

在用农杆菌浸染前,将茶树外植体预培养在含有PVP(polyvinylpyrrolidone,16g/L)的预培养基上2-3d可提高转化频率。优化后的茶树基因枪转化体系,即制弹程序:60 mg/1ml钨粉悬浮液10 l中加入1.6 l质粒DNA(1 g/l),再分别加入0.1 mol/L的亚精胺4 l,2.5 mol/L 的CaCl2 15 l,最后定容至48 l;每次轰击上样量为8-10 l。基因枪转化后抗性筛选2个月,抗性愈伤组织存活率为5.0%~12.1%。  相似文献   

Synchronization Using PGF2α and Estradiol With or Without GnRH for Timed Artificial Insemination in Dairy Cows     

Jae Kwan JEONG  Hyun Gu KANG  Tai Young HUR  Ill Hwa KIM 《The Journal of reproduction and development》2013,59(1):97-101

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Acute Changes in Central Corneal Thickness According to Experimental Adjustment of Intraocular Pressure in Normal Canine Eyes     

Young-Woo PARK  Man-Bok JEONG  Eui Ri LEE  Yesran LEE  Jae-Sang AHN  Soo-Hyun KIM  Kangmoon SEO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1479-1483

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