Identification of some main <Emphasis Type="Italic">Streptococcus iniae</Emphasis> associated proteins: relationship     

Fatima El Aamri  José Ángel Guillén  Daniel Padilla  Félix Acosta  Fernando Real 《Veterinary research communications》2017,41(2):85-95

The surface-associated proteins play a key role in bacterial physiology and pathogenesis, and are the major targets in the development of new vaccines. These proteins contribute to the adaptation of bacteria to different hosts and environments. To study differences at the genomic level, we first sequenced the whole genome of Streptococcus iniae from fish (IUSA-1 strain) and compared it to Streptococcus iniae from human (9117 strain), revealing a high similitude between both strains. To gain further insights into host- and environment-specific differences, we then studied proteins in silico and by High Performance Liquid Chromatography. This approach successfully identified 54 secreted and surface proteins, including several proteins involved in cell wall synthesis and transport of solutes, as well as proteins with yet unknown function. These proteins highlight as interesting targets for further investigation in the interaction between Streptococcus iniae and its environment. Results reported in this study have shown a first analysis about the predicted and experimental associated proteins of Streptococcus iniae isolated from two different hosts: human and fish.  相似文献   

Erratum to: Identification of some main <Emphasis Type="Italic">Streptococcus iniae</Emphasis> associated proteins: relationship     

Fatima El Aamri  José Ángel Guillén  Daniel Padilla  Jimena Bravo  Belinda Vega  Félix Acosta  Fernando Real 《Veterinary research communications》2017,41(2):97-97

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Validation of simple sequence repeats associated with quality traits in durum wheat     

Lamiae Amallah  Mouna Taghouti  Keltoum Rhrib  Fatima Gaboun  Moustapha Arahou  Rachida Hassikou  Ghizlane Diria 《Journal of Crop Science and Biotechnology》2016,19(2):137-150

Over recent years, quality has become an important commercial issue for durum wheat breeders. Modern breeding methods are most efficient for producing and supplying the best quality raw materials to the pasta industry. Here we assessed the effectiveness of molecular marker-assisted selection of quality traits in durum wheat. To this end, DNA and quality trait markers were jointly used to analyze quality-related traits in a durum wheat collection. A total of 132 durum wheat (Triticum turgidum ssp. durum) Mediterranean landraces, international lines, and Moroccan cultivars were analyzed for seven important qualityrelated traits including thousand-kernel weight (TKW), test weight (TW), gluten strength, yellow pigment (YP), and grain protein content (GPC). Additionally, 18 simple sequence repeat (SSR) markers previously reported to be associated with different quality traits were analyzed. Of these, 14 (78%) were polymorphic and four were monomorphic. There were between two and seven alleles per locus, with an average of four alleles per locus. The average phenotypic variation value (R²) ranged from 2.81 to 20.43%. Association analysis identified nine markers significantly associated with TKW, TW, and YP, followed by eight markers associated with GPC, six markers associated with yellow index b, four markers associated with brightness L, and three markers associated with SDS-sedimentation volume. This study highlights the efficiency of SSR technology, which holds promise for a wide range of applications in marker-assisted wheat breeding programs.  相似文献   

Levels of some antioxidant molecules and lipid peroxidation during in vivo transformation of the progenetic metacercaria of Clinostomum complanatum to ovigerous adult worms     

Rizvi A  Hasan S  Alam M  Zafar A  Fatima T  Shareef PA  Banu N  Saleemuddin M  Saifullah MK  Abidi SM 《Veterinary parasitology》2012,185(2-4):164-167

The levels of oxidative stress markers are an important indicator of the physiological state of the parasite and its host. In the present study levels of lipid peroxidation, glutathione S transferase, glutathione, superoxide dismutase and catalase were determined in the Clinostomum complanatum progenetic metacercaria, obtained from the fish peritoneum (a hypoxic habitat). The in vivo transformed ovigerous adult worms were obtained from the aerobic environment of the buccopharyngeal region of experimentally infected chickens. Levels of antioxidant molecules were also determined in the blood of experimentally infected chickens. An increase in the levels of lipid peroxidation, and a significant decrease in the levels of glutathione S transferase, glutathione, superoxide dismutase and catalase was observed in the infected host as compared to the controls. In the ovigerous worms, the levels of lipid peroxidation, glutathione S transferase, glutathione, superoxide dismutase were found to be significantly less than the levels observed in the progenetic metacercaria. Since the establishment of worm in the buccal cavity of the avian host would lead to its exposure to oxygen and the haematophagous nature of the parasite also exposes it to the free radicals in the host blood, the progenetic metacercaria has evolved to produce excess free radical scavenging molecules reserved to combat the oxidative stress encountered within the microhabitat of the definitive host.  相似文献   

Trace metal distribution in pelagic fish species from the north-west African coast (Morocco)     

Afandi  Imane  Talba  Sophia  Benhra  Ali  Benbrahim  Samir  Chfiri  Rachid  Labonne  Maylis  Masski  Hicham  Laë  Raymond  Tito De Morais  Luis  Bekkali  Mohammed  Bouthir  Fatima Zohra 《International Aquatic Research》2018,10(2):191-205

International Aquatic Research - In the current study, ten elements contents (Fe, Zn, Mn, Cu, Cr, Co, Ni, Cd, Pb and Hg) have been measured in muscle and liver of four pelagic fish species...  相似文献   

In vitro clonal propagation of mature Tectona grandis through axillary bud proliferation     

Fatima Shirin  Prem Kumar Rana  Asim Kumar Mandal 《Journal of Forest Research》2005,10(6):465-469

An in vitro clonal propagation procedure for mature Tectona grandis (teak) trees is described. Multiple shoots were induced from nodal segments through axillary bud proliferation. A shoot multiplication rate of 6.33 was achieved on Murashige and Skoog's (MS) medium supplemented with 10 μM 6-benzyladenine BA and 1 μM 1-naphthalene acetic acid (NAA) during every subculture cycle of 4 weeks. In vitro raised shoots could be successfully rooted (66.66%) on liquid MS medium supplemented with 15 μM NAA, with 1.60 roots per shoot, every 6 weeks of culture. In vitro hardening was carried out in sand soaked with half-strength MS medium (organic free). The plantlets were acclimatized first in a mist chamber and then in polybags in a mixture of soil, sand, and farmyard manure (1 : 1 : 1 v/v) in a shade house.  相似文献   

Serological,molecular detection and potential risk factors associated with camel brucellosis in Pakistan     

Sana Fatima  Iahtasham Khan  Amar Nasir  Muhammad Younus  Muhammad Saqib  Falk Melzer  Heinrich Neubauer  Hosny El-Adawy 《Tropical animal health and production》2016,48(8):1711-1718

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Effect of jasmonic acid on the induction of polyphenoloxidase and peroxidase activities in relation to date palm resistance against Fusarium oxysporum f. sp. albedinis     

Fatima Jaiti  Jean Luc Verdeil  Ismail El Hadrami 《Physiological and Molecular Plant Pathology》2009,74(1):84-90

Bayoud, caused by Fusarium oxysporum f. sp. albedinis (Foa), is the most damaging disease of date palm in Morocco. In the present study we have investigated the effect of jasmonic acid (JA) on two defence-related enzymes, namely peroxidases (POX) and polyphenoloxidases (PPO) in date palm seedlings root. Our data show that exogenous application of JA at a concentration of 50 μM increased the activity of both enzymes. The increase of POX activity in the presence of JA was much more important than that observed following infection with the pathogen. As compared to untreated plants, PPO activity was 2.2 and 1.3 times higher in BSTN and JHL cultivars respectively. In addition, PAGE analysis revealed increased band intensity of the major constitutive isoforms of POX and PPO in both JA-treated and Foa-treated seedlings. Close examination of symptomatic and asymptomatic plants showed that root tissues of symptomatic plants were massively colonized by Foa. Also, disease development in these plants appeared to involve a marked degradation of the host cell walls early during the process of pathogen invasion. In contrast, the presence of Foa in asymptomatic plants induced limited necrotic lesions (hypersensitive-reaction like lesions) that were probably involved in reducing the progression of the pathogen. Together, our findings indicate that JA is capable of enhancing date palm root resistance to infection by Foa via the activation of defence-related enzymes such as PPO and POX.  相似文献   

Field and controlled conditions screenings of some faba bean (Vicia faba L.) genotypes for resistance to the parasitic plant Orobanche crenata Forsk. and investigation of involved resistance mechanisms     

Briache  Fatima Zahra  Ennami  Mounia  Mbasani-Mansi  Joseph  Gaboun  Fatima  Abdelwahd  Rabha  Fatemi  Zain El Abidine  El-Rodeny  Walid  Amri  Moez  Triqui  Zine El Abidine  Mentag  Rachid 《植物病害和植物保护杂志》2019,126(3):211-224

Journal of Plant Diseases and Protection - The parasitic weed Orobanche crenata is a serious constraint to legumes crops in Mediterranean area. In Morocco, heavy O. crenata infestation level was...  相似文献   

Two-year study on combining ability and heterotic potential in functional traits under salt stress in upland cotton (Gossypium hirsutum)     

Muhammad Awais Farooq  Amir Shakeel  Waqas Shafqat Chattha  Muhammad Tahir 《Plant Breeding》2020,139(6):1221-1243

Salt tolerance is a physiologically and genetically complex trait controlled by multiple genes. To analyze the genetic basis of salt tolerance we evaluated 18 F₁ along with their nine parents under three salt stress levels i.e. control, 10 dS m⁻¹ and 15 dS m⁻¹ in the summer of 2017 and 2018. Data were recorded for the number of bolls, seed cotton yield, boll weight, Na⁺, K⁺, K⁺/Na⁺ ratio, H₂O₂, SOD, POD, CAT and TSP. Line × tester analysis indicated that the contribution of lines was greater than testers. Except for Na⁺, most of the traits were controlled by non-additive genes. With the rise of salt stress, SCA variances increased and were higher than GCA variances for most of the traits indicating the traits were controlled by a few largely dominant genes. KEHKSHAN, FH-118 and FH-114 were found good general combiner whereas the cross KEHKSHAN × FH-114 was a good specific combiner and also indicated significant better parent heterosis for most traits during two years under salt stress and can be utilized in a breeding programme for salt tolerance.  相似文献