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41.
Soil heavy metal pollution, influenced by both natural and anthropogenic factors, significantly reduces environmental quality. In this study, Cr, Ni, Cu, As, Cd, and Pb in eight different land-use soils from Patuakhali District in Bangladesh were assessed. Concentrations of Cr, Ni, Cu, As, Cd, and Pb in soils were 1-87, 5-271, 4-181, 0-80, 0.2-24.0, and 5-276 mg kg-1, respectively, measured using an inductively coupled plasma-mass spectrometer. The enrichment factor, pollution load index (PLI), and contamination factor (Cfi) of metal i were used to assess the ecological risk posed by metals in soils. The PLI ranged from 0.78 to 2.66, indicating baseline levels to progressive deterioration of soil due to metal contamination. However, Cfi of Cd ranged from 1.8 to 12.0, which showed that the studied soils were strongly impacted by Cd. Considering the severity of the potential ecological risk of a single metal, the descending order was Cd > As > Pb > Cu > Ni > Cr. Soils under all land uses showed moderate to very high potential ecological risk.  相似文献   
42.
A rhizobox experiment was conducted to compare the differences of soil potassium (K) distribution and absorption between two cotton (Gossypium hirsutum L.) genotypes under drought and K‐deficit conditions. Treatments included two levels of water (drought and optimum soil moisture: 25% and 35% volumetric water content) and K fertilizer rates (0 and 0.48 g potash kg?1 soil) applied to two cotton genotypes (namely HEG and LEG). Both the genotypes showed significant differences in total K accumulation without exogenous K addition. After absorption, soil content of the readily available potassium (RAK) decreased rapidly. This promoted the conversion of the mineral K into slowly available potassium (SAK). Drought significantly decreased the cotton growth and K use efficiency, and thereby reduced the effect of K fertilizer. Consequantly, the contents of RAK and SAK were greatly increased. However, K bioavailability was decreased under water stress conditions. Differences in root parameters and soil microorganisms between two cotton genotypes were significantly increased and had marked relations with available soil K contents. This study provides important information for understanding the mechanism of K use efficiency, especially under water and K stress.  相似文献   
43.
Bifidobacterium animalis subsp. lactis bb12 and B. longum 20099 were used to hydrolyze isoflavone glycosides (IG) to biologically active forms, which are isoflavone aglycones (IA), in soymilk (SM) prepared from soy protein isolate (SPI) and SM supplemented with 0.5% (w/v) of lactulose (SML). Supplementation of lactulose significantly ( p < 0.05) enhanced the viable counts of B. animalis subsp. lactis bb12 and B. longum 20099 up to 2.34 and 2.15 log CFU/mL, respectively. Bifidobacterium animalis subsp. lactis bb12 and B. longum 20099 utilized 3.32 and 3.75 mg/mL of lactulose at 24 h of incubation, respectively. Supplementation of lactulose also appeared to be a key factor in decreasing the pH of SML. The biotransformation of IG to IA was enhanced significantly by 6.8-17.1% and 12.8-13.5% in SML by B. animalis subsp. lactis bb12 and B. longum 20099, respectively. However, the presence of lactulose in SML showed the stimulating effect on B. longum 20099 only after 12 h of incubation. Isoflavone aglycones ranged from 69.5 to 77.1% of total isoflavone compounds in SML after incubation.  相似文献   
44.
Xu Q  Song X  Xu L  Yan R  Shah MA  Li X 《Veterinary parasitology》2008,156(3-4):319-323
A fusion DNA vaccine co-expressed Eimeria tenella TA4 and chicken IL-2 (chIL-2) was constructed and its efficacy against E. tenella challenge was observed. TA4 gene of E. tenella and chIL-2 gene were cloned into expression vector pcDNA3.1 and pcDNA4.0c in different forms, producing vaccines pcDNA3.1-TA4-IL-2, pcDNA3.1-TA4 and pcDNA4.0c-IL-2. The expression of aim genes in vivo was detected by RT-PCR and western blot. Animal experiment was carried out to evaluate the immune efficacy of the vaccines. Results indicated these DNA vaccines were successfully constructed and the antigen genes could be expressed effectively in vivo. The animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease ratio. The ACI of pcDNA3.0-TA4-IL-2 group was 192, higher than that of pcDNA3.1-TA4 group. The results suggested that TA4 was an effective candidate antigen for vaccine and co-expression of cytokine with antigen was an alternative method to enhance DNA vaccine immunity.  相似文献   
45.
Contagious caprine pleuropneumonia (CCPP) is a serious disease of goats, occasionally sheep and wild ruminants, caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). The disease is characterized by severe serofibrinous pleuropneumonia, very high morbidity (~100%), and mortality (80–100%). CCPP affects goats in more than 40 countries of the world thereby posing a serious threat to goat farming around the globe. The characteristic clinical signs of CCPP are severe respiratory distress associated with sero-mucoid nasal discharge, coughing, dyspnea, pyrexia, pleurodynia, and general malaise. In later stages, severe lobar fibrinous pleuropneumonia, profuse fluid accumulation in pleural cavity, severe congestion of lungs and adhesion formation is observed. Mycoplasmal antigen interactions with host immune system and its role in CCPP pathogenesis are not clearly understood. CCPP is not a zoonotic disease. Diagnosis has overcome cumbersome and lengthy conventional tests involving culture, isolation, and identification by advanced serological (LAT, cELISA) or gene-based amplification of DNA (PCR, RFLP, and hybridization) and sequencing. The latex agglutination test (LAT) is rapid, simple, and better test for field and real-time diagnosis applicable to whole blood or serum and is more sensitive than the CFT and easier than the cELISA. Moreover, the studies on antibiotic sensitivity and exploration of novel antibiotics (fluoroquinolones, macrolides) can help in better therapeutic management besides preventing menace of antibiotic resistance. Re-visiting conventional prophylactic measures focussing on developing novel strain-based or recombinant vaccines using specific antigens (capsular or cellular) should be the most important strategy for controlling the disease worldwide.  相似文献   
46.
Veterinary Research Communications - Avian polyomavirus (APV) infection, also called as budgerigar fledgling disease (BFD) causes various health problems in many psittacine species which may cause...  相似文献   
47.
Tropical Animal Health and Production - Therapeutic management of contagious caprine pleuroneumonia (CCPP) involves mostly the use of oxytetracycline followed by enrofloxacin and rarely tylosin. In...  相似文献   
48.
The aim of this investigation was to examine the pharmacokinetics and mammary excretion of erythromycin administered to lactating ewes (n = 6) by the intravenous (i.v.), intramuscular (i.m.) and subcutaneous (s.c.) routes at a dosage of 10 mg/kg. Blood and milk samples were collected at pre-determined times, and a microbiological assay method was used to measure erythromycin concentrations in serum and milk. The concentration-time data were analysed by compartmental and non-compartmental kinetic methods. The serum concentration-time data of erythromycin were fit to a two-compartment model after i.v. administration and a one-compartment model with first-order absorption after i.m. and s.c. administration. The elimination half-life (t(1/2beta)) was 4.502 +/- 1.487 h after i.v. administration, 4.874 +/- 0.296 h after i.m. administration and 6.536 +/- 0.151 h after s.c. administration. The clearance value (Cl tot) after i.v. dosing was 1.292 +/- 0.121 l/h/kg. After i.m. and s.c. administration, observed peak erthyromycin concentrations (Cmax) of 0.918 +/- 0.092 microg/ml and 0.787 +/- 0.010 microg/ml were achieved at 0.75 and 1.0 h (Tmax) respectively. The bioavailability obtained after i.m. and s.c. administration was 91.178 +/- 10.232% and 104.573 +/- 9.028% respectively. Erythromycin penetration from blood to milk was quick for all the routes of administration, and the high AUC milk/AUC serum (1.186, 1.057 and 1.108) and Cmax-milk/Cmax-serum ratios reached following i.v., i.m. and s.c. administration, respectively, indicated an extensive penetration of erythromycin into the milk.  相似文献   
49.
Soil sodicity development is a process that depends nonlinearly on both salt concentration and composition of soil water. In particular in hot climates, soil water composition is subject to temporal variation due to dry–wet cycles. To investigate the effect of such cycles on soil salinity and sodicity, a simple root zone model is developed that accounts for annual salt accumulation and leaching periods. Cation exchange is simplified to considering only Ca/Na exchange, using the Gapon exchange equation. The resulting salt and Ca/Na-balances are solved for a series of dry/wet cycles with a standard numerical approach. Due to the nonlinearities in the Gapon equation, the fluctuations of soil salinity that may be induced, e.g. by fluctuating soil water content, affect sodicity development. Even for the case that salinity is in a periodic steady state, where salt concentrations do not increase on the long term, sodicity may still grow as a function of time from year to year. For the longer term, sodicity, as quantified by Exchangeable Sodium Percentage (ESP), approaches a maximum value that depends on drought and inflowing water quality, but not on soil cation exchange capacity. Analytical approaches for the salinity and sodicity developing under such fluctuating regimes appear to be in good agreement with numerical approximations and are very useful for checking numerical results and anticipating changes in practical situations.  相似文献   
50.
Zeylanicobdella arugamensis (Annelida: Hirudinea), a marine parasitic leech, is currently affecting different species of cultured groupers, hybrid groupers, snappers and sea bass in Malaysia. Dillenia suffruticosa (Dilleniaceae), a medicinal plant found in Sabah, has been selected in our experiment to kill the leeches as a natural control method. The leech‐infested hybrid groupers were collected from aquaculture facilities, and the isolated leeches were challenged against methanol extract of D. suffruticosa leaves. The experiment was carried out using various concentrations of the extracts such as 25, 50 and 100 mg/ml. The methanol extract showed significant antiparasitic activity against Z. arugamensis with 100% mortality at a concentration of 100 mg/ml in 14.39 ± 3.75 min., followed by 50 and 25 mg/ml in 32.97 ± 9.29 and 41.77 ± 5.40 min., respectively. The phytochemical composition of the extract was determined using GC‐MS analysis to understand the nature of the principal compounds responsible for its antiparasitic properties. The leaves of D. suffruticosa demonstrated the presence of different bioactive compounds of various natures with varying percentages. Thus, it could be revealed that the methanol extract of D. suffruticosa mainly contains vital phytochemical compounds and showed an effective antiparasitic activity against the harmful leeches of hybrid groupers.  相似文献   
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