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321.
Factors associated with being a bovine-virus diarrhoea (BVD) seropositive dairy herd were studied in a case-control study of 314 dairy herds in the M[oslash]re and Romsdal County of Norway. Information was collected through a mailed questionnaire, and associations were modeled using conditional logistic regression after selecting variables by a best-subset procedure. Purchasing of animals, use of common pasture, herd-to-herd contact over pasture fences, purchasing cattle with insufficient health (about BVD) documentation, and not using dairy advisors were associated with higher risk. In addition, younger farmers were more likely to have a bovine-virus diarrhoea seropositive herd than older farmers. ‘Other animal traffic', including use of common animal housing in the summer months and exchange of calves between farmers were also risk factors. Collectively, these factors could explain 51% of the seropositive herds.  相似文献   
322.
The activated clotting time (ACT) is commonly used in veterinary medicine as an assessment of dysfunction within the intrinsic clotting cascade. Performing the test requires little techincal expertise and no special equipment except for a heating block or constant temperature waterbath, neither of which is routinely found in veterinary practices. The purpose of this study was to determine whether performing the test using a human axilla as the heat source was accurate for both normal dogs and clinically ill dogs (with prolonged ACT's) when compared to using a heating block as the head source. The results of this study reveal that the axillary method of ACT determination has acceptable clinical agreement with the heating block method. Thus, the axillary method of ACT determination is an acceptable alternative when no constant temperature heating source is available.  相似文献   
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The reproductive biology of 315 cobia, Rachycentron canadum, from northeastern Australia was studied for an 18-month period. Cobia ranged from 181 to 1,470 mm FL (0.06–55 kg). Length–frequency distributions of males and females did not differ significantly. The sex ratio of females to males was 2.18:1. Histological data showed that cobia developed hydrated oocytes during a protracted spawning season between September and June. Gonadosomatic index peaked from October to December, coinciding with the monsoon or “wet” season. Estimated length at first maturity for female cobia was 671 mm FL. Length at 50% maturity (L 50) for females was estimated at 784 mm FL (1–2 years of age). Batch fecundity ranged from 577,468 to 7,372,283 eggs with a mean of 2,877,669 (± SD 1,603,760) eggs. Relative batch fecundity was 249 eggs per g, and no relationship between relative fecundity and fork length was found. There was a significant positive relationship between the total number of eggs produced and fork length. Spawning frequency, estimated by the post-ovulatory follicle method, was 7.6 days. Based on the detection of hydrated oocytes in fish caught at night, cobia most likely spawn at night. Cobia also feed throughout the spawning period. This is the first report on the reproductive biology of cobia in Australian waters, and provides valuable data for future population assessments of cobia throughout the Indo-Pacific.  相似文献   
326.
由于禽流感一直独占家禽新闻的头版头条,几乎让人们相信已无其他值得顾虑的禽病了。然而,事实恰恰与此相反。养禽业连续不断地受到那些不应该被忽视的健康问题困扰。如何才能有效预防这些健康问题呢?  相似文献   
327.
Pale, soft, and exudative (PSE) meat has been recognized for decades. Fast glycolysis during early post-mortem stage while the muscle temperature is still high is the cause of PSE meat. To elucidate the molecular mechanism underlying this fast glycolysis in muscle to become PSE meat, post-mortem ATP metabolism, fructose-2,6-diphosphate content, and the activities of AMPK, glycogen phosphorylase, and pyruvate kinase were examined in post-mortem muscle. Earlier and faster post-mortem AMPK activation was responsible for the significantly lower pH and higher lactic acid accumulation (p<0.05) seen in PSE muscle, which resulted in the occurrence of PSE meat. In muscle that became PSE meat, AMPK was activated at 0 h post-mortem and reached maximal activation at 0.5 h post-mortem, whereas AMPK reached maximal activation at 1 h post-mortem in the normal pork loin. Higher fructose-2,6-diphosphate content (p<0.05) was detected in PSE muscle compared to normal muscle at early post-mortem stage. However, no difference in the activities of glycogen phosphorylase and pyruvate kinase, rate-controlling enzymes in glycogenolysis and glycolysis, respectively, was detected between PSE and normal pork loins. Because fructose-2,6-diphosphate is a product of phosphofructokinase-2 (PFK-2), these data suggest that AMPK regulates post-mortem glycolysis through its phosphorylation and activation of PFK-2, which then up-regulates the activity of phosphofructokinase-1 (PFK-1), a key rate-controlling enzyme in glycolysis. Early AMPK activation in PSE muscle is associated with early consumption of ATP, because higher AMP and IMP contents and lower ATP content were detected in PSE meat compared to normal meat. Other mechanisms causing early AMPK activation in PSE meat may exist, which warrants further investigation.  相似文献   
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