苹果梨中多酚氧化酶酶学特性的研究   总被引：26，自引：2，他引：26  

程建军  马莺  杨咏丽  凌海波  焦明晶  庄严 《园艺学报》2002,29(3):261-262

 以苹果梨中的多酚氧化酶为研究对象, 对其酶的活性变化及其特性的研究表明, 苹果梨中的多酚氧化酶的最适pH 值是4. 6 , 最适温度是40 ℃; 亚硫酸氢钠、维生素C、柠檬酸对苹果梨中多酚氧化酶的抑制作用依次减弱, 硫酸钠几乎无抑制作用。不同底物和同底物不同浓度影响多酚氧化酶特性。  相似文献   

早熟、优质、耐贮梨新品种新梨7号选育研究   总被引：7，自引：0，他引：7  

刘建萍  阎春雨  程奇  王新建  吴翠云 《果树学报》2002,19(1):36-38

以库尔勒香梨为母本,早酥梨为父本,有性杂交选育成新梨７号品种。该品种同时兼备优质、早熟与耐贮藏性;该品种遗传了父本的早熟性,比父本更早熟１５~２０ｄ,盛花期~果实可食期约８０~９５ｄ,属于早熟品种;且遗传了母本品质性状的质地酥脆、耐贮、清香、阳面红晕,比母本果个大,果心小。果柄木质化,丰产抗风。新梨７号果实贮藏期的可溶性固形物、果实重量、果实硬度和果实前期腐烂率的变化与库尔勒香梨基本相似,但耐热能力高于母本。凡适宜香梨、早酥、苹果梨和巴梨种植的地区,都适宜该品种的种植。  相似文献   

果树抗寒性的遗传与育种研究进展   总被引：23，自引：0，他引：23  

沈洪波  陈学森  张艳敏 《果树学报》2002,19(5):292-297

冻害胁迫下果树体内的水分从生长的组织流失迁移,在其周围区域形成冰核保护生长组织,不形成冰核发生冻害;ABA和Ca(2+)在植物低温胁迫的信号转导中起重要作用;DTA法测定的LTH温度与花芽的半致死温度一致;果树杂种后代的抗寒性遗传具有母性遗传特点。还总结了近年来发展起来的果树抗寒性鉴定的有效方法以及部分抗寒资源,以期为果树抗寒育种的亲本选择及不同品系抗寒力的早期鉴定提供参考依据。  相似文献   

Preparation of gfp-bcl-X_L-contained recombinant adenovirus vector by the homologous recombination in bacteria     

LUO Yan  TANG Shi-bo  LI Xiang-hong  LIU Shuang-xin 《园艺学报》2002,18(12):1462-1467

AIM: To prepare gfp-bcl-X_L-contained recombinant adenovirus(rAd-gfp-bcl-X_L).METHODS: Bcl-X_L gene was amplified from pEGFP-C₃-bcl-X_L, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-X_L. Then pAdTrack-CMV-bcl-X_L was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-X_L and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-X_L into 293 cells. PCR test indicated that the recombinant Ad contained bcl-X_L gene. The titer of the purified rAd-gfp-bcl-X_L was 6.5×10¹² PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-X_L. This affords a good gene transfer vector for the gene therapy in human’s diseases.  相似文献   

Adult rat and human bone marrow mesenchymal stem cells differentiate into neurons with Musk's polypeptide     

XIAO Qing-zhong  LI Hao-wei  WEN Guan-mei  HUANG Shao-hua ZHANG Xiu-ming  LI Yan  LI Shu-nong 《园艺学报》2002,18(10):1179-1182

AIM: To investigate the differentiation from adult rat and human bone marrow mesenchymal stem cells (BMMSCs) into neuron with musk polypeptide (Mu-P).METHODS: Adult rat and human BMMSCs were induced with Mu-P.Neuron-specific enolase (NSE),neurofilament (NF),Nestin,glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry.RESULTS: Simple methods with Mu-P induced adult rat and human BMMSCs exhibiting a neuronal phenotype,expressing Nestin at 3 hours to 5 hours,and expressing NE and NF at 5 hours to 7 days.But the neuron-like cells didn't express the glial astrocyte marker GFAP.CONCLUSION: Adult rat and human BMMSCs can be induced to differentiate into neurons with Mu-P.  相似文献   

Effect of Jiawei Sini Decoction on glucocorticoid receptor in thymocytes of chronic psychological stress rats     

YAN Can  SHI Ya-fei  LI Yan  WU Zhi-wei  WU Li-li  PAN Yi 《园艺学报》2002,18(12):1494-1496

AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND C₁, group treated by ginsenosides C₂. The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C₁ and C₂ were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C₁ and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats.  相似文献   

Effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts     

ZHANG Li-rong  XIANG Peng  XIA Wen-jie  CHEN Zhen-guang  ZHANG Xiu-ming  LI Yan  LI Shu-nong 《园艺学报》2002,18(8):896-899

AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK.  相似文献   

The genetic susceptibility of HLA-DRB1 alleles in esophageal neoplasm of Hubei Han Chinese     

LIN Jun  SUN Jie  ZHOU Yan  HUANG Xing  XIONG Ping  WANG Ya-ping  DENG Chang-sheng 《园艺学报》2002,18(9):1112-114

AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal neoplasm in Hubei Han Chinese. METHODS: HLA-DRB1 gene polymorphism in 42 patients with esophageal neoplasm and 136 normal control subjects was studied by PCR and sequence. RESULTS: Allele frequency of HLA-DRB1 *0901 allele was significantly higher in esophageal cancer patients than those in normal controls(0.2500 vs 0.1397, P =0.028; the odds ratiO².053; etiologic fraction 0.1282).There were no association between the rested HLA-DRB1 alleles with patients. CONCLUSION: Individuals carrying HLA-DRB1 *0901 may be susceptible to esophagealo carcinoma, its nucleotide sepuence approachs to the corresponded allele sequence(exoN²)published in GenBank.  相似文献   

喀喇昆仑山盖孜河水文水资源特性分析   总被引：2，自引：1，他引：2  

李燕  李红斌  王连有 《干旱区研究》2003,20(4):272-275

喀喇昆仑山河流自有水文资料记载以来 ,近 10年洪灾发生频率明显高于以往 ,人民的生命财产损失程度也大幅度增加。在分析了盖孜河流域的径流及气候多年变化过程后 ,认为洪灾频发的原因是近年来流域内降水及气温呈上升趋势 ,导致高山冰川退缩 ,河流径流增加 ,洪灾增大、频率增高 ;小区域多年气候变化过程从侧面印证了我国西北地区气候由暖干向暖湿变化。  相似文献   

Computer-aided consultation and identification system for insect pests infesting fruits     

Chen Nai-zhong  Shen Zuo-rui 《EPPO Bulletin》2003,33(2):321-323

A computer database has been developed on insect pests infesting fruits, for use by phytosanitary inspectors and pest risk analysts in China. A total of 304 species is covered. Besides basic nomenclatural, biological and distributional information on each pest, the database includes dangerousness scores calculated according to a new system, and an identification system based on external characters.  相似文献