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Identification of metabolites in plasma and urine of Uruguayan propolis-treated rats 总被引:1,自引:0,他引:1
Kumazawa S Shimoi K Hayashi K Ishii T Hamasaka T Nakayama T 《Journal of agricultural and food chemistry》2004,52(10):3083-3088
Propolis is a resinous substance collected by honeybees from various plant sources. It is extensively used in food and beverages to improve health and prevent diseases such as heart disease, diabetes, and cancer. To investigate the absorption and metabolism of the components in propolis, in the present study, we administered ethanol extracts of Uruguayan propolis (poplar type propolis) orally to rats and analyzed their plasma and urine by high-performance liquid chromatography with photodiode array and mass spectrometric detection. After deconjugation of the components by beta-glucuronidase/sulfatase treatment of the specimen, pinobanksin 5-methyl ether, pinobanksin, kaempferol, chrysin, pinocembrin, and galangin were detected in plasma of rats orally administered propolis. These compounds were detected also in urine after beta-glucuronidase/sulfatase treatment. Furthermore, pinobanksin 5-methyl ether, pinobanksin, chrysin, pinocembrin, and galangin were present in the urine also in free form. These results suggest that flavonoids in propolis are metabolized and circulate in the body after oral administration of propolis. 相似文献
215.
The rate of sugar formation from aspartate-14C(U) and alanine-L-14C was examined under various light intensities in three C4-plants. The results obtained were as follows. The rates of sugar formation from aspartate-14C(U) became larger in the following order, Paspalum urvillei, Egragrostis ferrunginea, and Zoysia japonica. This order agreed well with the order of their photosynthetic rates measured by gas analyzer. In all the C4-plants, there were three steps in the sugar formation curve from aspartate-14C(U). At first, sugar linearly increased with an increase in the light intensity up to 20 klux. Second, from 20 to 40 klux, it hardly increased with an increase in the light intensity. Third, above 40 klux, it increased linearly again. On the other hand, the plateau did not exist between 20 and 40 klux in the sugar formation curves from alanine-l-14C in any of the cases, and sugar continued to increase with an increase in the light intensity up to 80 klux. At low light intensities, the amount of CO2 released from aspartate-14C(U) and alanine-l-14C correlated well with the magnitude of the dark respiration in the C4-plants. At a high light intensity, however, CO2 release closely correlated with the thickness of mesophyll layers surrounding the vascular bundle sheath. The thicker the mesophyll layers were, the smaller the release of CO2 became. From this evidence, we conclude that the mesophyll layers play a vital role in refixation of the internal CO2 in the light. 相似文献
216.
Shigenori Aomine 《Soil Science and Plant Nutrition》2013,59(4):571-580
The movement of ammonium due to diffusion in paddy soils in Taiwan was investigated in the laboratory. The movement of ammonium in flooded soils was inversely proportional to the concentration of ammonium in the soil solution, which was a function of the quantity of ammonium present, the content of solid matter in a unit volume of soil (bulk density), and the adsorptive property of soil. Although there was a considerable variation in the value of both the ammonium adsorption and bulk density of soils, the adsorptive property was more variable. Therefore, the adsorptive property seemed to be the most influencial factor on the movement of ammonium in flooded soils, where the property had a close relation to the cationexchange capacity. The bulk density of flooded soils in the field could be reproduced in glass tubes under percolating condition in the laboratory. The values of bulk density determined on paddy soils after harvesting or on air-dry soils were considerably different from those in the flooded soils in the field. A simple determination method of bulk density was proposed for paddy soils. 相似文献
217.
It is well established that the presence of clay in an enzyme-substrate system may reduce the activity of enzymes (1-3,5,6,8,11-14). This inhibition by the clay would be principally caused by the adsorption of enzyme and/or substrate on the clay particle. In the previous papers (1–3), the activity of some enzymes was found to be inhibited in various degrees by clays. The enzyme appears to be adsorbed on the clay particle in various ways according to the nature of adsorbate as well as adsorbent. This suggests that the enzyme is adsorbed by the clay so as not to combine with the substrate, or that the adsorbed enzyme molecule has a configuration different from that of a free enzyme molecule, reducing the activity of the enzyme. 相似文献
218.
Shigenori Aomine 《Soil Science and Plant Nutrition》2013,59(3):105-113
The principal volcanic ash soils of Chile is evidently the “trumao” soil that covers the Central Valley from an area north of Chillan (36°30′S) to Puerto Montt (4l03l′S), and which is very similar to Japanese Andosols in its morphological, chemical and physical properties (19, 20). The different conides of Antuco, Chillan, Villarrica, Llaima, Osorno, and others in this part of the Cordillera indicate that the parent materials of the soils could have various origins, not only horizontally but also vertically. Moreover, glaciations have further complicated the composition of the parent materials of the soils (Langohr, R., personal communication). Even earthquakes have influences on the soils in some districts (21). Irrespective of the origin of soil parent materials, certain trends appear to exist in some chemical and physical properties of the soils from north to south (18), suggesting a difference in fertility and organic matter. 相似文献
219.
Taiichiro Hattori Kaori Sonobe Shinobu Inanaga Ping An Shigenori Morita 《Journal of plant nutrition》2013,36(6):1046-1058
Effects of silicon (Si) application on photosynthesis of solution-cultured cucumber seedlings were investigated under osmotic stress and unstressed conditions. In unstressed conditions, silicon application had no effect on growth and photosynthetic parameters. The responses of the photosynthetic parameters to abruptly imposed osmotic stress did not differ between silicon treatments. After 1 week exposure to osmotic stress, growth reduction was observed, but it was less severe in seedlings grown with silicon than in those without silicon. Although there were no differences between silicon treatments in stomatal conductance, transpiration rate, cuticular transpiration, or xylem sap exudation rate under osmotic stress, leaf intercellular carbon dioxide (CO2) concentration was significantly lower and photosynthetic rate tended to be higher in seedlings supplied with silicon. These results suggested that the silicon-induced alleviation of growth reduction under osmotic stress in cucumber was due to amelioration of stress-induced damage of leaf tissues rather than to improvement of leaf water status. 相似文献
220.
María Del Mar Ortega-Villaizán Romo Shigenori Suzuki Masamichi Nakajima Nobuhiko Taniguchi 《Fisheries Science》2006,72(1):33-39
ABSTRACT: The barfin flounder has been recently declared to be rare, as the number of wild individuals in Japan has greatly reduced since 1975. The Japanese National Center for Stock Enhancement started the stocking program for this species on 1987. In the present study, microsatellite DNA loci were applied in order to determine the pedigree of the hatchery-produced juveniles for stock enhancement. Additionally, using six polymorphic microsatellite markers, the accuracy to assign dyads to categories of kinship was estimated using the relatedness estimator ( R XY ), similarity indexes (individual genetic identity [GI] and proportion of shared alleles [ P S ]), identification of kin groups using unweighted pair group method with arithmetic mean (UPGMA) clustering based on interindividual genetic distances (1- R XY , 1- P S , and 1-GI), a likelihood ratio approach, and partition methods to separate individuals into cohorts. The results were compared with the pedigree relationship previously obtained from parentage analysis. Estimation of the kinship relationship between individuals could be better inferred by means of UPGMA dendrogram clustering based on 1-GI or 1- P S as interindividual genetic distances, or using computer software to perform partitioning of set of individuals. 相似文献