Cloning of a Genomic DNA Encoding Caffeoyl-coenzyme A 3-<Emphasis Type="Italic">O</Emphasis>-methyltransferase of Citrus     

Alfredo Gryciuk ALMEIDA  Shinji TSUYUMU 《Journal of General Plant Pathology》2000,66(2):144-148

In recent studies, we found that Apll (a PthA homologue) bound to three citrus proteins. Amino acid sequence analysis revealed that one of the target proteins was homologous to that of S-adenosyl-l-methionine : trans-caffeoyl-coenzyme A 3-O-methyltransferase (CCoAMT), an enzyme which is specific for the substrate trans-caffeoyl-CoA and catalyzes the synthesis of trans-feruloyl-CoA. From the consensus nucleotide sequences of CCoAMT genes, primers were chosen for PCR amplification of this gene from citrus total DNA. Two selected DNA fragments of 1.0 kb and 2.0 kb were obtained. These fragments were used as the probe to screen a citrus library. One clone, pCCl00, contained a 1.0-kb SalI fragment that hybridized to the probes. The nucleotide sequence of this fragment was determined in both directions. In this fragment, there was an open reading frame of 232 amino acids interrupted by an intron of 106 nt, and the deduced amino acid sequence had 95.9% homology to tobacco CCoAMT. Southern blot analysis of total citrus DNA showed that four EcoRI fragments hybridized to the probes, suggesting the presence of more than one copy of CCoAMT in the citrus DNA. Received 4 November 1999/ Accepted in revised form 26 November 1999  相似文献   

Lowering the incineration temperature of fishery waste to optimize the thermal decomposition of shells and spines     

Kobatake  Hidekazu  Kirihara  Shinji 《Fisheries Science》2019,85(3):573-579

Fisheries Science - A part of fishery waste, such as scallop or oyster shells, are utilized for fertilizer, feed, soil conditioner, or fishing ground preparations, and most of the shells from...  相似文献   

Spontaneous polyploidization in critically endangered <Emphasis Type="Italic">Acipenser mikadoi</Emphasis>     

Miloš Havelka  He Zhou  Seishi Hagihara  Masaki Ichimura  Takafumi Fujimoto  Etsuro Yamaha  Shinji Adachi  Katsutoshi Arai 《Fisheries Science》2017,83(4):587-595

We investigated the source of spontaneous polyploidization in the critically endangered Acipenser mikadoi. Fourteen sib progeny of A. mikadoi and 11 hybrids between an A. mikadoi female and a Huso dauricus male, all showing atypically high ploidy, were analysed. Parent assignment based on five highly polymorphic microsatellite markers confirmed spontaneous duplication of maternal chromosome sets via retention of the second polar body to be the source of spontaneous polyploidization. To our knowledge, this provides the first evidence of the maternal origin of spontaneous polyploidization in A. mikadoi. Factorial correspondence analysis of the multilocus microsatellite genotypes placed the parent fish of spontaneous polyploids in clearly delineated clusters of A. mikadoi and H. dauricus, and parent fish had mitochondrial control region haplotypes corresponding to their presumed species. Thus, parent fish were confirmed to be of pure genetic origin, and hybridization did not promote the observed spontaneous polyploidization.  相似文献   

Expression of podoplanin in various types of feline tumor tissues     

Satoshi KAMOTO  Masahiro SHINADA  Daiki KATO  Masaya TSUBOI  Sho YOSHIMOTO  Ryohei YOSHITAKE  Shotaro ETO  Namiko IKEDA  Yosuke TAKAHASHI  Yuko HASHIMOTO  James CHAMBERS  Kazuyuki UCHIDA  Shinji YAMADA  Mika K. KANEKO  Ryohei NISHIMURA  Yukinari KATO  Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1795

Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. Podoplanin is also expressed in cancer-associated fibroblasts and induces tumor malignancy. The objective of this study was to evaluate podoplanin expression in various types of feline tumor tissues. Immunohistochemical analysis revealed that podoplanin was expressed in cells of 13/15 (87%) squamous cell carcinomas and 5/19 (26%) fibrosarcomas. Moreover, cancer-associated fibroblasts expressed podoplanin in most tumor types, including 18/21 (86%) mammary adenocarcinoma tissues. Our findings demonstrate that various types of feline tumor tissues expressed podoplanin, indicating the importance of the comparative aspects of podoplanin expression, which may be used as a novel research model for podoplanin biology.  相似文献   

Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan     

Akio SUZUKI  Kazuhei SAWADA  Temuulen ERDENEBAT  Takeshi YAMASAKI  Minoru TOBIUME  Kinuyo SUGA  Motohiro HORIUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1735

There has been no report on Chronic wasting disease (CWD) cases in Japan to date; however, there is concern about the geographic spread of CWD. To clarify the CWD status in Japan, we conducted CWD monitoring using real-time quaking-induced conversion (RT-QuIC) assay which can detect the low level of CWD prions. A total of 690 obex samples collected from sika deer and Reeves’s muntjac in Hokkaido and Honshu was tested for CWD prions. No CWD-positive cases were found, suggesting that CWD is nonexistent in Japan. Our results also indicate that RT-QuIC assay is useful for continuous monitoring of CWD. Furthermore, nucleotide sequence analysis of the PrP gene revealed sika deer in Japan harbor CWD susceptible allele.  相似文献   

Age and growth of red tilefish Branchiostegus japonicus in two fishery grounds off the Kaetsu region,Niigata Prefecture,northern Sea of Japan     

Iseki  Tomoaki  Yagi  Yuta  Uehara  Shinji  Kajihara  Naoto  Fujii  Tetsuo 《Fisheries Science》2022,88(6):667-676

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