Correction to: Catalytic pyrolysis of cellulose in sulfolane with some acidic catalysts     

Haruo Kawamoto  Shinya Saito  Wataru Hatanaka  Shiro Saka 《Journal of Wood Science》2018,64(6):880-880

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Practical method combining loop-mediated isothermal amplification and bait trap to detect <Emphasis Type="Italic">Pythium helicoides</Emphasis> from hydroponic culture solutions     

Noriyuki Miyake  Hirofumi Nagai  Shinro Kato  Masashi Matsusaki  Shiro Fukuta  Reiko Takahashi  Ryoji Suzuki  Yasushi Ishiguro 《Journal of General Plant Pathology》2017,83(1):1-6

Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium-selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P. helicoides in hydroponic culture systems.  相似文献   

Influence of reaction atmosphere on the liquefaction and depolymerization of wood in an ionic liquid, 1-ethyl-3-methylimidazolium chloride     

Atsushi Nakamura  Hisashi Miyafuji  Shiro Saka 《Journal of Wood Science》2010,56(3):256-261

The influence of reaction atmosphere on the liquefaction and depolymerization of wood in an ionic liquid, 1-ethyl-3-methylimidazolium chloride ([C2mim][Cl]), has been systematically studied. The wood samples were treated with [C2mim][Cl] at 120°C under various atmospheres such as oxygen, nitrogen, and carbon dioxide, both dried and humidified. The percentage of residue after the treatment shows that oxygen considerably accelerates the liquefaction of wood in [C2mim][Cl], and humidity hardly affects liquefaction under any atmosphere. Gel permeation chromatography (GPC) and high performance liquid chromatography (HPLC) analyses on the solubilized compounds in [C2mim][Cl] indicate that oxygen and humidity enhance the depolymerization of the wood component. Thus, the reaction atmosphere was revealed to influence, and 1be capable of controlling, the reaction of wood in [C2mim][Cl].  相似文献   

Extractives of muirapiranga (<Emphasis Type="Italic">Brosimun</Emphasis> sp.) and its effects on the vibrational properties of wood     

Kazuya Minato  Yuji Konaka  Iris Bremaud  Shiro Suzuki  Eiichi Obataya 《Journal of Wood Science》2010,56(1):41-46

The potential of muirapiranga (Brosimun sp.) as a substitute material for violin bows was estimated in terms of vibrational properties, and the influence of extractives on the vibrational properties was examined. The loss tangent of muirapiranga was somewhat higher and the specific dynamic Young’s modulus was rather lower than the respective values for pernambuco, which is regarded as the best material for violin bows. Therefore, muirapiranga is a poorer bow material in terms of vibrational properties. Impregnation of crude extractives from muirapiranga decreased the loss tangent of other wood specimens. The main compounds of the extractives were identified as xanthyletin and luvangetin. Impregnations of isolated xanthyletin and commercially available methoxsalen, which was tested as an analogue of luvangetin, markedly decreased the loss tangent of other wood specimens. Methoxsalen and xanthyletin differ from conventional loss tangent-decreasing substances, namely protosappanin B and hematoxylin, in terms of water insolubility and the absence of hydroxyl groups. From the similarity in molecular characteristics of loss tangent-decreasing substances found so far, restriction of molecular motion due to an impregnated substance in the wood matrix is suggested as one loss tangent-decreasing mechanism.  相似文献   

Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation     

Takano K  Watanabe-Takano H  Suetsugu S  Kurita S  Tsujita K  Kimura S  Karatsu T  Takenawa T  Endo T 《Science (New York, N.Y.)》2010,330(6010):1536-1540

Insulin-like growth factor 1 (IGF-1) induces skeletal muscle maturation and enlargement (hypertrophy). These responses require protein synthesis and myofibril formation (myofibrillogenesis). However, the signaling mechanisms of myofibrillogenesis remain obscure. We found that IGF-1-induced phosphatidylinositol 3-kinase-Akt signaling formed a complex of nebulin and N-WASP at the Z bands of myofibrils by interfering with glycogen synthase kinase-3β in mice. Although N-WASP is known to be an activator of the Arp2/3 complex to form branched actin filaments, the nebulin-N-WASP complex caused actin nucleation for unbranched actin filament formation from the Z bands without the Arp2/3 complex. Furthermore, N-WASP was required for IGF-1-induced muscle hypertrophy. These findings present the mechanisms of IGF-1-induced actin filament formation in myofibrillogenesis required for muscle maturation and hypertrophy and a mechanism of actin nucleation.  相似文献   

First report of broccoli foot rot caused by Rhizoctonia solani AG-2-2 IV and pathogenicity comparison of the pathogen with related pathogens     

Tomoo Misawa  Masaharu Kubota  Jun Sasaki  Shiro Kuninaga 《Journal of General Plant Pathology》2015,81(1):15-23

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Outbreak of larval Echinococcus multilocularis infection in Japanese monkey (Macaca fuscata) in a zoo, Hokkaido: western blotting patterns in the infected monkeys     

Sato C  Kawase S  Yano S  Nagano H  Fujimoto S  Kobayashi N  Miyahara K  Yamada K  Sato M  Kobayashi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):133-135

A high prevalence of larval Echinococcus multilocularis (Em) infection was found in zoo primates in Hokkaido, Japan. In October 1997, a Japanese monkey (Macaca fuscata) died and histopathologically diagnosed as alveolar hydatidosis. Serum samples were collected from the remaining Japanese monkeys and examined for antibodies against Em by enzyme-linked immunosorbent assay and western blotting. Serological tests showed 12 more animals of the remaining 57 monkeys were possibly infected. Ultrasonography revealed that nine of these 12 animals had a cystic lesion in the liver. The band patterns of western blotting in the monkeys were very similar to those in human.  相似文献   

Development of a male sterile eggplant by utilizing the cytoplasm of Solanum virginianum and a biparental transmission of chloroplast DNA in backcrossing     

Md. Mizanur Rahim Khan  Shiro Isshiki   《Scientia Horticulturae》2008,117(4):316-320

To develop a male sterile line of eggplant (Solanum melongena L.) utilizing the cytoplasm of S. virginianum, an interspecific F₁ hybrid between S. virginianum and S. melongena ‘Senryo Nigou’ was continuously backcrossed to S. melongena ‘Uttara’ using ‘Uttara’ as a recurrent pollen parent and four backcross generations, BC₁, BC₂, BC₃ and BC₄ were produced. All the plants in backcross generations were anther indehiscent although the F₁ hybrid, S. virginianum and S. melongena were dehiscent. Pollen stainability with acetic carmine and in vitro germination ability of pollen in all the backcross progenies were quite lower than those of the parental species. Fruit set percentage, number of seeds per fruit and seed germination rate were high in all the backcross progenies. The present results indicate that anther indehiscent type of functional male sterile line of eggplant could be developed by utilizing the cytoplasm of S. virginianum. PCR-RFLP analysis of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were performed to identify the organelle inheritance. The F₁ hybrid and all the backcross progenies displayed maternal inheritance of mtDNA. The cpDNA of the examined single BC₁ plant exhibited the recombinant cpDNA pattern of the parental F₁ hybrid and ‘Uttara’, indicating occurrence of the biparental inheritance of cpDNA. As all the BC₂, BC₃ and BC₄ progenies showed the same recombinant cpDNA patterns of the BC₁ plant, the recombinant cpDNA might be stable and harmonize with the nuclear genome of S. melongena. The present male sterile line can contribute to expand the male sterility source of eggplant.  相似文献   

ISSR variations in eggplant (Solanum melongena L.) and related Solanum species     

Shiro Isshiki  Naoko IwataMd. Mizanur Rahim Khan 《Scientia Horticulturae》2008

Inter-simple sequence repeat (ISSR) analysis was performed in eight cultivars of eggplant (Solanum melongena) and 12 accessions in eight related Solanum species to evaluate the applicability of this analysis for assessing the phylogenetic relationships and identifying cultivars. A total of 552 polymorphic amplified bands were obtained from 34 of the 100 primers tested, and the percentage of polymorphisms was 99.1%. Cluster analysis based on the ISSR markers classified the Solanum species into seven groups: (i) S. melongena; (ii) S. aethiopicum and S. anguivi; (iii) S. incanum; (iv) S. violaceum and S. kurzii; (v) S. macrocarpon; (vi) S. virginianum and (vii) S. torvum. Combining the ISSR markers obtained by a few of the 34 primers was enough for distinguishing of the eight cultivars of eggplant. This ISSR analysis was demonstrated to be available for the phylogenetic study and the cultivar identification.  相似文献   

中国内蒙自治区马饲育环境土壤中马红球菌的分布     

丁壮  Shinji TAKAI  Hiroo MADARAME  常爽  黄海楠  霍晓伟  高明华  谭忠田  高双成  Fumiko HATORI  Yukako SASAKI  Tsutomu KAKUDA  Shiro TSUBAKI 《中国兽医学报》2008,28(1):40-44

对中国内蒙古自治区饲育马土壤环境中马红球菌的分布知之甚少;为此,在中国内蒙古通辽近郊、内蒙古南部锡林郭勒盟大草原、内蒙古东部呼伦贝尔大草原等马场共收集了108份土壤样品进行了马红球菌的检测。结果,锡林郭勒盟大草原和呼伦贝尔大草原的马红球菌分离率为25.9%～30.0%,通辽近郊土壤中分离率高达82.3%。这说明在通辽近郊马红球菌的分离菌数是草原土壤中马红球菌分离菌数的10倍。应用PCR技术检测了488株的毒力相关基因,相对分子质量分别为15000～17000（VapA）和20000（VapB）。所有的分离株都未检出毒力相关基因。这些无毒力分离株的质粒资料显示,各种大小的潜在质粒的发生率为13.3%～21.5%。本研究结果截然不同于我们新近对蒙古国的调查结论：马红球菌在蒙古国乌兰巴托的马群中不存在。造成这一差异的原因可能在于蒙古国的游牧生活和内蒙古的非游牧生活。  相似文献