Utilization of extruded soybean meal for Japanese flounder Paralichthys olivaceus juveniles     

Sanshiroh  SAITOH  Shunsuke  KOSHIO  Hiroshi  HARADA  Kazunori  WATANABE  Takeshi  YOSHIDA  Shin-ichi  TESHIMA  Manabu  ISHIKAWA 《Fisheries Science》2003,69(5):1075-1077

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Relationship between the reduction of tetrazolium salt XTT and DNA strand breakage with aminosugars     

Shimamura T  Takamori A  Ukeda H  Nagata S  Sawamura M 《Journal of agricultural and food chemistry》2000,48(4):1204-1209

Dihydropyrazine derivatives formed by the self-condensation reaction of D-glucosamine have the DNA breaking activity. To establish the monitoring method of the biological active dihydropyrazines, we investigated the relationship between the XTT (3'-[1-[(phenylamino)-carbonyl]-3, 4-tetrazolium]bis(4-methoxy-6-nitro)benzensulfonic acid hydrate) reducibility and the DNA breaking activity of aminosugars. Aminosugar in 50 mM sodium phosphate buffer (pH 7.4) was incubated at 37 degrees C. At a given time, the XTT reducibility and the DNA breaking activity of the incubated aminosugar were measured. Both XTT reducibility and DNA breaking activity showed a maximum value within 1-4 h after the incubation and then gradually decreased with the incubation time. Superoxide anion was suggested to involve in both of the DNA breaking activity and the XTT reducibility by the addition of the radical scavengers into those assay mixtures. The quantity of remaining covalently closed circular DNA and the XTT reducibility of all aminosugars showed a good correlation (r = 0.825, n = 26). This means that the XTT assay is applicable for the monitoring of those biologically active products derived from aminosugars when the participation of superoxide anion in DNA scission is recognized.  相似文献   

Interactive Effects of Vitamin C and E Supplementation on Growth,Fatty Acid Composition,and Lipid Peroxidation of Sea Cucumber,Apostichopus japonicus,Fed with Dietary Oxidized Fish Oil     

Jian Gao  Shunsuke Koshio  Manabu Ishikawa  Saichiro Yokoyama  Daisuke Nose  Tongjun Ren 《Journal of the World Aquaculture Society》2013,44(4):536-546

A feeding trial was conducted to determine the interactive effects of vitamin C (ascorbic acid, AsA) and E (α‐tocopherol, α‐Toc) supplementation with dietary oxidized fish oil (OFO) on the growth performance, whole‐body AsA, and α‐Toc concentrations and fatty acid composition of juvenile sea cucumber. In a 9‐wk feeding trial, juveniles (average weight: 0.6 ± 0.1 g) were cultured in twenty‐four 50‐L tanks (30 juveniles per tank) in triplicate, and fed with eight test diets containing two levels of OFO (8.9 and 156.9 meq/kg) with varying levels of vitamin C (500 and 1000 mg AsA equivalents/kg diet) and E (100 and 200 mg α‐Toc equivalents/kg diet) supplementation, respectively. Body weight gain of sea cucumber was significantly reduced by dietary OFO, while mortality and whole‐body thiobarbituric acid‐reactive substances value were increased significantly. Increasing dietary vitamin C and E levels significantly increased whole‐body α‐Toc and AsA concentrations, respectively. A high level of vitamin E combined with OFO led to consumption of AsA. Even with supplementation of a large dose of vitamin C and/or E in diets, growth performance could not be improved, probably due to the high levels of vitamins in the control diets.  相似文献   

The Effect of Different Dietary Energy Levels on Growth and Survival of Eyestalk Ablated and Intact Juvenile Lobsters Homarus americanus     

Shunsuke  Koshio  Ron K.  O'Dor  John D.  Castell 《Journal of the World Aquaculture Society》1990,21(3):160-169

When fed formulated diets containing 45% protein derived from the rock crab Cancer irroratus , wheat gluten, gelatin, lipids adjusted from 3–14% and carbohydrates adjusted from 5–24%, the average survival of bilaterally eyestalk ablated juvenile lobsters and intact controls were 91% and 99%, respectively over a 90-day experiment. Photopenad (13 light: 11 dark vs 3 light: 21 dark) did not have a significant effect on growth or survival of ablated or intact lobsters. The optimal digestible energy levels and protein/energy ratio for the greatest growth in both ablated and intact lobsters were 3.7 to 3.8 (kcal/g dry) and 0.12 [g protein/(kcal/g)], respectively. The best feed conversion ratios (0.97 and 1.40 diet given/weight gain) for ablated and intact lobsters were obtained with diets containing 3.83 kcal/g and 3.63 kcal/g diet, respectively. The present study also demonstrated that crab protein served as a good protein source for intact and ablated lobsters.  相似文献   

Surgical correction of ventricular septal defect with aortic regurgitation in a dog     

Shimizu M  Tanaka R  Hoshi K  Hirao H  Kobayashi M  Shimamura S  Yamane Y 《Australian veterinary journal》2006,84(4):117-121

An 8-month-old entire Miniature Dachshund, weighing 4.2 kg, was presented for examination following delvelopment of a cough. Ventricular septal defect had been diagnosed tentatively in its infancy on the basis of a cardiac murmur detected by auscultation and echocardiography. Echocardiography using a B mode right parasternal long-axis view showed a defect at the atrioventricular junction and a thickened cusp of the aortic valve prolapsing into the defect. Colour-flow Doppler showed shunt blood flow across the defect at the level of the atrioventricular junction, from left to right. The sinus of Valsalva was dilated, with turbulent blood flow. Aortic regurgitation was also observed. Cardiac catheterisation studies confirmed the diagnosis of a supracristal ventricular septal defect with aortic regurgitation. Despite medication with digoxin, enalapril and aminophylin, started from the first admission, left ventricular internal dimensions gradually increased, and fractional shortening of the left ventricle gradually decreased. Surgery, with the aid of extracorporeal circulation, to close the ventricular septal defect, was performed 1 year after the initial examination. The aortic valve was left untreated. Postoperatively, the systolic murmur disappeared. Shunt flow from the left to the right ventricle was no longer observed on echocardiography, however there was still a small amount of aortic regurgitation during diastole visualised with colour-flow Doppler echocardiography. The prolapse of the cusp of the aortic valve on B-mode echocardiography was no longer observed and thickening of the cusp had not progressed. Left ventricular function measurement using M mode echocardiography showed a reduced left ventricular volume overload with reduced left ventricular internal dimensions and increased fractional shortening. The cough was relieved and no follow-up medication was scheduled. Early surgical closure of the ventricular septal defect improved the patient's condition and controlled prolapse and thickening of the aortic valve.  相似文献   

Comparative study of anti-oncogenic microRNA-145 in canine and human malignant melanoma     

Noguchi S  Mori T  Hoshino Y  Yamada N  Nakagawa T  Sasaki N  Akao Y  Maruo K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):1-8

MicroRNA-145 (miRNA-145; miR-145) is aberrantly expressed in most of human cancers and plays a significant role in carcinogenesis and cancer progression. In the current study, we focused on how miR-145 plays a role in canine and human malignant melanomas. MiR-145 was significantly downregulated in canine malignant melanoma tissues and canine melanoma cell lines, as well as human melanoma cell lines tested. The ectopic expression of miR-145 showed a significant growth inhibition in both canine and human melanoma cells tested, and the effect was achieved partly by suppressing c-MYC in canine melanoma LMeC and in human melanoma A2058 and Mewo cells. At the same time, a suppressive tendency on cell migration in canine melanoma KMeC cells and significant suppression of cell migration in human melanoma A2058 cells by suppressing FASCIN1 were also found. These findings suggest that miR-145 acts as a tumor suppressor in both canine and human malignant melanomas.  相似文献   

Hatch timing of two subarctic salmonids in a stream network estimated by otolith increments     

Kevin A. Fitzgerald  Matt R. Haworth  Kevin R. Bestgen  Collin J. Farrell  Shunsuke Utsumi  Osamu Kishida  Hiromi Uno  Yoichiro Kanno 《Fisheries Management and Ecology》2021,28(6):507-515

Hatch timing in autumn-spawning stream salmonids is poorly understood in the subarctic region because snow cover prevents direct sampling of cryptic early life stages. Otolith micro-increment analysis was used to infer hatch dates of white-spotted charr Salvelinus leucomaenis (Pallas) and masu salmon Oncorhynchus masou (Brevoort) in a mainstem-tributary network in northern Japan. Accuracy and precision were validated by ageing hatchery individuals with known hatch date ranges. In July 2018, 93 wild young-of-the-year white-spotted charr and 81 masu salmon were collected and aged. Masu salmon hatched, on average, 24 days earlier (mean = February 8) than white-spotted charr (March 4), and hatch dates spanned a minimum of 2 months for each species. In masu salmon, hatch dates of individuals collected in the mainstem were nearly 3 weeks earlier than those in a tributary. This study provided knowledge on intra- and inter-specific variation in hatch timing of native salmonids in a subarctic stream network.  相似文献   

Surgical correction of subvalvular aortic stenosis using cardiopulmonary bypass in a dog     

Hirao H  Hoshi K  Kobayashi M  Shimizu M  Shimamura S  Tanaka R  Machida N  Maruo K  Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):559-562

A three-month-old male Golden Retriever had symptoms including exercise intolerance, dyspnea and syncope and was diagnosed with subvalvular aortic stenosis. Cardiac catheterization revealed a left ventricular-aortic systolic pressure gradient of 90 mm Hg. Surgical correction of the condition was achieved using cardiopulmonary bypass. The subvalvular fibrous lesion was resected through an aortotomy. The stenosis was dilated from 8.5 mm to 12.0 mm in diameter. Postoperatively the dog was asymptomatic. Seven months after surgery, the pressure gradient decreased to 44 mm Hg. However, after another three months, the dog died suddenly without any premonitory signs. Postmortem examination revealed that pathologic changes caused by increased left ventricular pressure overload were not severe.  相似文献   

Urinary albumin and transferrin as early diagnostic markers of chronic kidney disease     

Hiroto MAEDA  Kazuyuki SOGAWA  Kazuko SAKAGUCHI  Saori ABE  Wataru SAGIZAKA  Shunsuke MOCHIZUKI  Waka HORIE  Toshifumi WATANABE  Yui SHIBATA  Mamoru SATOH  Akihiro SANDA  Fumio NOMURA  Jun SUZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):937-943

Feline renal diseases are increasingly noted in veterinary practice. It is important to diagnose and identify the pathological basis of renal dysfunction accurately at an early stage, but there are only a few reports on this area in clinical veterinary medicine. We investigated the efficacy of measurement of urinary albumin (u-Alb) and urinary transferrin (u-Tf) for early diagnosis using 5-µl urine samples collected noninvasively by catheterization from normal (IRIS stage I) cats and cats with stage I chronic kidney disease (CKD). The u-Alb levels in normal and stage I CKD cats were 6.0 ± 4.5 and 11.2 ± 8.4 mg/dl, respectively, and the u-Tf levels were 0.09 ± 0.42 and 0.52 ± 0.79 mg/dl, respectively. Based on ROC curve analysis, the sensitivity and specificity of u-Alb and u-Tf were higher than those of the currently used biomarker, the plasma creatinine level. The sensitivity of u-Alb was higher than that of u-Tf, whereas the specificity of u-Tf was higher than that of u-Alb. The validity of the threshold albumin level (20 mg/dl) was confirmed by measurements using SDS-PAGE. Since leakage of u-Tf in urine precedes leakage of u-Alb, inclusion of u-Tf in biochemistry tests may be appropriate for IRIS staging as a diagnostic marker of early diagnosis of renal disorder in cats.  相似文献   

Treatment of a unicameral bone cyst in a dog using a customized titanium device     

Ayami NOJIRI  Hideo AKIYOSHI  Fumihito OHASHI  Atsuki IJIRI  Osamu SAWASE  Tomiharu MATSUSHITA  Mitsuru TAKEMOTO  Shunsuke FUJIBAYASHI  Takashi NAKAMURA  Tsutomu YAMAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):127-131

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