Polymorphism of insulin‐like growth factor 1 gene is associated with breast muscle yields in chickens     

Shinichi SATO  Tsuyoshi OHTAKE  Yoshinobu UEMOTO  Yumi OKUMURA  Eiji KOBAYASHI 《Animal Science Journal》2012,83(1):1-6

Insulin‐like growth factor‐1 (IGF1) plays an important role in muscle development in chickens. In this study, an F2 chicken population of 362 individuals, obtained from an intercross between high breast muscle yield line males and low breast muscle yield (LB) line females, was constructed for investigating the associations between IGF1 gene and breast muscle yields. The IGF1 sequence was investigated in the grandparents. There were no differences in the exon sequences. However, sequence analysis of the IGF1 promoter revealed a known single nucleotide polymorphism (g.570C > A) in LB line grandparents. PCR – restriction fragment length polymorphism was used for screening the F2 population, which was evaluated for body weight (BW), carcass weight (CW), breast muscle weight (BMW), and breast fillet weight (BFW). Significant associations with the polymorphism were detected for BMW, BFW, BMW% and BFW%, although there were no associations between the polymorphism and BW or CW. The allelic effect on BMW, BFW, BMW% and BFW% acted in additive and dominance modes. We confirmed that the g.570C > A polymorphism is significantly associated with breast muscle yields in the F2 population. Therefore, this polymorphism in the IGF1 gene may help improve breast muscle yields by marker‐assisted selection.  相似文献   

Effects of porcine leptin receptor gene polymorphisms on backfat thickness,fat area ratios by image analysis,and serum leptin concentrations in a Duroc purebred population     

Yoshinobu UEMOTO  Takashi KIKUCHI  Hikaru NAKANO  Shuji SATO  Tomoya SHIBATA  Hiroshi KADOWAKI  Kazuo KATOH  Eiji KOBAYASHI  Keiichi SUZUKI 《Animal Science Journal》2012,83(5):375-385

The leptin receptor (LEPR) gene is considered a candidate gene for fatness traits. It is located on SSC 6 in a region in which quantitative trait loci (QTLs) for backfat thickness (BF), fat area ratios, and serum leptin concentration (LEPC) have previously been detected in a Duroc purebred population. The objectives of the present study were to identify porcine LEPR polymorphisms and examine the effects of LEPR polymorphisms on fatness traits in this same population. The Duroc pigs (226 to 953 pigs) were evaluated for BF, fat area ratios using image analysis, and LEPC. A total of seven single nucleotide polymorphisms (SNPs) in the full‐length LEPR coding region were identified in pigs from the base population. Four non‐synonymous SNPs of the LEPR gene and 15 microsatellite markers on SSC 6 were then genotyped in all pigs. During candidate gene analysis, we detected significant effects of the non‐synonymous SNP c.2002C>T in exon 14 on all traits. In fine mapping analysis, significant QTLs for BF, fat area ratios, and LEPC were detected near the LEPR gene in the same region. These results indicated that the c.2002C>T SNP of LEPR has a strong effect on BF, fat area ratios and LEPC.  相似文献   

Analysis of L-type amino acid transporter in canine hepatocellular carcinoma     

Kikumi OGIHARA  Yuko NAYA  Reiichiro SATO  Ken ONDA  Hideharu OCHIAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):527-534

Analysis of L-type amino acid transport expression of hepatocellular carcinoma cells (HCCs) of the dog was performed. The leucine transport activity of canine HCCs was 0.628 ± 0.018 nmol/mg protein/min. The inhibitor of LAT 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (BCH) reduced 90% of the activity at 1 mM. The deduced amino acid sequences of canine LAT2, LAT3 and LAT4 were well conserved in mammalians, exhibiting 89, 88 and 77% homology, respectively. RT-PCR revealed distinct LAT1 expression compared with normal hepatocytes. Western blotting analysis confirmed the potent LAT1 expression in canine HCCs but not hepatocytes, and real-time RT-PCR analysis indicated that canine HCCs possessed 28 times higher LAT1 expression than hepatocytes. These results indicated that the leucine transport activity of canine HCCs was due to LAT1.  相似文献   

Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans,animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis     

Noriko IDO  Kaori IWABUCHI  Yusuke SATO’O  Yasuo SATO  Masaru SUGAWARA  Gakuji YAEGASHI  Masaru KONNO  Masato AKIBA  Kiyoshi TANAKA  Katsuhiko OMOE  Ikuo UCHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):609-613

Fifty-one Salmonella enterica serovar 4,[5],12:i:- (S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S. Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA) in order to investigate their genetic diversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34 profiles (Simpson’s diversity indices [DI]=0.94, 0.96 and 0.97), respectively. No correlations were detected between MLVA clustering and PFGE clustering or phage typing. These results suggested that S. 4,[5],12:i:- originated from multiple S. Typhimurium ancestors. Two cattle and one pig isolates showing identical phage types as well as PFGE and MLVA profiles to human isolates S. 4,[5],12:i:- suggested the existence of the links between human infections and animal reservoirs.  相似文献   

Anti-Müllerian hormone as an indicator of hemi-castrated unilateral cryptorchid horses     

Harutaka MURASE  Shigeaki SAITO  Tomohiko AMAYA  Fumio SATO  Barry Allen BALL  Yasuo NAMBO 《Journal of Equine Science》2015,26(1):15-20

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Activation of bacteriophage by urea     

SATO GH 《Science (New York, N.Y.)》1956,123(3203):891-892

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Accumulation of phenylalanine by a phenylalanineless mutant of Escherichia coli     

KATAGIRI M  SATO R 《Science (New York, N.Y.)》1953,118(3061):250-251

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Purification and characterization of a nucleoplasmin-like protein from carp (Cyprinus carpio) eggs     

KOJI SATO  AKIKO IIHARA  NAOYUKI SAKAI  FUMINARI ITO  MOTOYOSHI NOMIZU  NORIO NISHI 《Fisheries Science》2002,68(4):904-912

ABSTRACT: Nucleoplasmin, first isolated from Xenopus laevis eggs, promotes nucleosome assembly. Hereby, we have purified a nucleoplasmin-like protein from carp ( Cyprinus carpio ) eggs using ion exchange and subsequent gel filtration columns. The protein was recognized by a polyclonal antiserum against Xenopus laevis nucleoplasmin and had an amino acid composition similar to other member of the nucleoplasmin family proteins. Partial amino acid sequences from the cyanogen bromide (CNBr)-cleaved fragments showed high homology with Xenopus nucleoplasmin. The protein was also found to form an oligomeric complex and to be phosphorylated. Moreover, this protein promoted sperm nuclear decondensation as well as that of nucleoplasmin from Xenopus laevis eggs. These results suggest that the fish protein isolated here is a member of nucleoplasmin family.  相似文献   

Differential expression of two gonadotropin (GTH) β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel Anguilla japonica     

HIROAKI  SUETAKE  KATAAKI  OKUBO  NARUMI  SATO  YASUTOSHI  YOSHIURA  YUZURU  SUZUKI  KATSUMI  AIDA 《Fisheries Science》2002,68(2):290-298

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Effect of oral administration of L-pipecolic acid on food intake in chicks and mice     

Tomo TAKAGI  Ryuichi ANDO  Atsushi OHGUSHI  Tomonori NAKANISHI  Hazizul HUSSAIN-YUSUF  Ryoji ONODERA  Hiroyuki SATO  D. Michael DENBOW  Mitsuhiro FURUSE 《Animal Science Journal》2003,74(2):101-104

It has been demonstrated that L-pipecolic acid (L-PA), a major metabolic intermediate of L-lysine (L-Lys) in the brain, is involved in the functioning of the γ-aminobutyric acid-ergic system. Previous study has shown that intracerebroventricular injection of L-PA suppressed feeding and induced sleep-like behavior in neonatal chicks. The present study examines whether the action of L-PA was induced by gavage in both chicks and mice. Oral administration of L-PA significantly inhibited food intake at 2 h after treatment in neonatal chicks, although no significant effect of L-Lys was detected. In mice, oral L-PA suppressed food intake compared to the control after 2 h of treatment. It was concluded that L-PA was effective for suppression of food intake after oral administration in both avian and mammalian species.  相似文献