Construction of an Expression System for Bioactive IL-18 and Generation of Recombinant Canine Distemper Virus Expressing IL-18     

Yuxiu LIU  Hiroki SATO  Masahiro HAMANA  Navita Anisia MOONAN  Misako YONEDA  Xianzhu XIA  Chieko KAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(9):1241-1248

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Isolation of Escherichia coli Strains with AcrAB–TolC Efflux Pump-Associated Intermediate Interpretation or Resistance to Fluoroquinolone, Chloramphenicol and Aminopenicillin from Dogs Admitted to a University Veterinary Hospital     

Toyotaka SATO  Shin-ichi YOKOTA  Risa ICHIHASHI  Tomoka MIYAUCHI  Torahiko OKUBO  Masaru USUI  Nobuhiro FUJII  Yutaka TAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(7):937-945

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Bovine growth hormone gene polymorphism affects stress response in Japanese Black cattle     

Noriko Tachi  Sigefumi Tanaka  Astrid Ardiyanti  Kazuo Katoh  Shusuke Sato 《Animal Science Journal》2014,85(6):722-728

We investigate the associations between growth hormone (GH) gene polymorphism and behavioral and physiological responses to stressors and learning ability in Japanese Black cattle. Flight distance test was conducted in the first experiment. Steers with haplotype C of GH gene polymorphism avoided human approaches at a significantly greater distance than ones without haplotype C (C: 1.9 ± 0.9, non‐C: 1.0 ± 0.2 m, P < 0.05). An open‐field test was conducted in the second experiment. Behavioral responses did not differ significantly between steers with and without haplotype C. Increases of heart rates to dropping of iron pipes was significantly higher in steers with haplotype C (C:161.7 ± 21.8, non‐C:130.7 ± 31.3%, P < 0.05). Despite basal serum concentrations not being different between steers with and without haplotype C, serum cortisol in blood sampling immediately after severe confinement in a race tended to be higher in steers with haplotype C (P = 0.1). The maze test was conducted as the third experiment. There was no difference in performance in the maze test between steers with and without haplotype C. It is concluded that genetic polymorphism of GH may affect stress responses through GH concentration in steers.  相似文献   

The response of adipose tissues to Mycoplasma pulmonis and Sendai virus infection in C57BL/6 and DBA/2 mice     

Tussapon BOONYARATTANASOONTHORN  Keisuke SATO  Yuko OKAMATSU-OGURA  Masami MORIMATSU  Takashi AGUI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):403

Adipose tissues in mammals are categorized into white and brown adipose tissues in which cellular morphology, cell functions, and tissue distribution are different. White adipose tissue (WAT) plays a major role in energy reservation, while brown adipose tissue (BAT) mainly relates to the thermoregulation of the body. One interesting function of adipose tissue is the response to the infection, especially the pathogens that cause pneumonia. We have previously reported that DBA/2 (D2) mice are susceptible to pathogens causing pneumonia, Mycoplasma (M.) pulmonis and Sendai virus (SeV), whereas C57BL/6 (B6) mice are resistant to them. Furthermore, morphological alteration of mediastinal fat tissue (MFT) was seen after infection of M. pulmonis in D2 mice but not in B6 mice. In this study, we aimed to exhibit the difference in adipose tissue response in other areas, including interscapular brown adipose tissue (iBAT), inguinal white adipose tissue (ingWAT), and perigonadal WAT (perigoWAT) between resistant strain, B6 and susceptible strain, D2 after challenging them with M. pulmonis and SeV. Compared with B6 mice, D2 mice showed an increase in fat-associated lymphoid cluster in MFT, an increase in BAT in both iBAT and ingWAT after M. pulmonis and SeV infection. The results of this study indicate that pneumonia caused by M. pulmonis and SeV infection induces browning of adipocyte, suggesting that BAT plays a role in pathogen infection and inflammation.  相似文献   

Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae)     

Takuma ARIIZUMI  Shiro MURATA  Sotaro FUJISAWA  Masayoshi ISEZAKI  Naoya MAEKAWA  Tomohiro OKAGAWA  Takumi SATO  Eiji OISHI  Akira TANENO  Satoru KONNAI  Kazuhiko OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):558

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Anti-lipopolysaccharide antibody administration mitigates ruminal lipopolysaccharide release and depression of ruminal pH during subacute ruminal acidosis challenge in Holstein bull cattle     

Hitoshi MIZUGUCHI  Tomoki IKEDA  Yumi WATANABE  Shiro KUSHIBIKI  Kentaro IKUTA  Yo-Han KIM  Shigeru SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):905

The effects of anti-lipopolysaccharide (LPS) antibody on rumen fermentation and LPS activity were investigated during subacute ruminal acidosis (SARA) challenge. Eleven Holstein cattle (164 ± 14 kg) were used in a 3 × 3 Latin square design. Cattle were fed a roughage diet on days −11 to −1 (pre-challenge) and day 2 (post-challenge), and a high-grain diet on days 0 and 1 (SARA challenge). For 14 days, 0-, 2-, or 4-g of anti-LPS antibody was administered once daily through a rumen fistula. Ruminal pH was measured continuously, and rumen fluid and blood samples were collected on days −1, 0, 1, and 2. Significantly lower ruminal LPS activity on day 1 was observed in the 2- and 4-g groups than those in the 0-g group. In addition, significantly higher 1-hr mean ruminal pH on SARA challenge period (days 0 and 1) was identified in the 4-g group than in the 0-g group. However, rumen fermentation measurements (total volatile fatty acid [VFA], VFA components, NH₃-N and lactic acid) and peripheral blood metabolites (glucose, free fatty acid, beta-hydroxybutyrate, total cholesterol, blood urea nitrogen, aspartate aminotransferase and gamma-glutamyl transferase) were not different among the groups during the experimental periods. Therefore, anti-LPS antibody administration mitigates LPS release and pH depression without the depression of rumen fermentation and peripheral blood metabolites during SARA challenge in Holstein cattle.  相似文献   

Identifying the factors that affect the willingness of pig farmers to participate in the electronic prescription system     

Kyoko FUJIMOTO  Yuko ENDO  Takashi YOKOYAMA  Hiroaki SUGINO  Takeshi SATO  Takeshi HAGA  Nozomi KAWAMURA  Katsuaki SUGIURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):747

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