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41.
Severe wilt with spots and/or leaf and stem blight were found on a scrophulariaceous flowering plant, Nemesia strumosa, grown in Kagawa Prefecture, Japan, in February 1999. Wilted plants had numerous lesions and died early. A mitosporic fungus isolated repeatedly from the diseased plants was identified as Colletotrichum fuscum and was demonstrated to cause the disease. N. strumosa is a new host for C. fuscum, which has been known to attack foxglove (Digitalis spp.). The present disease was named “anthracnose of N. strumosa” as a new disease. Received 10 October 2000/ Accepted in revised form 11 January 2001  相似文献   
42.
Several amino acids have effects on mental function, including sedative, antidepressant‐like and anxiolytic‐like effects. However, the influence of integrated amino acid nutrition as protein constituents on mental function remains unclear. Therefore, the purpose of the present study was to compare the influence of chicken, pork and beef protein extracts on brain monoamine metabolism in mice. Changes in monoamine levels and their turnover rates in the brain were induced by different protein sources. In particular, chicken protein group showed the highest norepinephrine levels in the hippocampus and hypothalamus, and beef protein extract caused an activation of the serotonergic system in the hypothalamus, although there were no significant differences in amino acid compositions of these protein extracts. Therefore, it was revealed that amino acid compositions in dietary protein did not induce alteration in monoamine metabolism. However, there were differences in small molecular peptides, such as creatine, carnosine and anserine levels in animal protein extracts. In conclusion, monoamine metabolism was altered by dietary protein sources. However, it was indicated that the alteration in monoamine metabolism may be independent from amino acid compositions in dietary protein. In addition, alteration in monoamine metabolism depending on the dietary protein sources may be induced by small molecular peptides.  相似文献   
43.
Several studies have shown a relationship between depression and animal protein intake. To evaluate whether the difference of dietary chicken protein levels induces an antidepressant‐like effect and potentiates acute antidepressant effects, three levels of dietary chicken protein were used as the representative animal protein with imipramine used as the antidepressant. In addition, the effects of dietary chicken protein on brain metabolism were evaluated. Open field test (OFT) and forced swimming test (FST) were conducted on the 27th and 28th days, respectively. OFT and FST were not influenced by both imipramine and dietary protein levels. However, characteristic effects of imipramine treatment on brain monoamine metabolism were observed in the cerebral cortex and hypothalamus. In addition, dietary protein significantly increased taurine and L‐ornithine levels even though these amino acids were not contained in the diets. In conclusion, the metabolism of several amino acids in the plasma and brain were altered by dietary chicken protein.  相似文献   
44.
Although our previous report demonstrated that adiponectin and AdipoR1 gene expressions changed among different lactation stages in the bovine mammary gland, its in vivo kinetics remain unclear in ruminant animals. In this study, we investigated the changes in circulating concentrations of adiponectin, as well as other metabolic hormones and metabolites, (i) during the periparturient period and (ii) among different lactation stages, in Holstein dairy cows. In experiment 1, serum adiponectin concentrations increased after parturition. Serum insulin concentrations were lower in the postpartum than prepartum period, whereas serum growth hormone (GH) concentrations increased in the postpartum period. Serum nonesterified fatty acids (NEFA) levels were increased during the postpartum period and were dependent on the parity. In experiment 2, there was no significant difference in plasma adiponectin concentrations among lactational stages. Plasma insulin concentrations tended to be lower in early lactation while plasma GH levels tended to be higher. Plasma NEFA concentrations were significantly lower in mid‐ and late‐lactation stages than non‐lactation stages. These findings indicate that elevation of serum adiponectin might be involved in energy metabolism just around parturition, and might exert its action through regulation of receptor expression levels in target tissues in each lactational stage in Holstein dairy cows.  相似文献   
45.
In this retrospective study, two observers independently reviewed thoracic imaging studies of 39 dogs with confirmed histiocytic sarcoma. The most common findings were intrathoracic lymphadenopathy, identified by the first and second observers in 82.1% and 87.2% of dogs, respectively, and pulmonary masses (74.4% and 82.1%). Right middle lung lobe masses were significantly more common than masses in any other lung lobe (P<0.0013), with the majority having a ventral distribution. Sternal and tracheobronchial lymphadenopathy were significantly more common than cranial mediastinal lymphadenopathy (P‐values of 0.0002 and 0.012, respectively). Interobserver agreement regarding distribution of lymphadenopathy and pulmonary masses was good (κ=0.64 and 0.75, respectively). Other findings included pulmonary nodules, pleural effusion, and abnormal pulmonary patterns. In patients with CT examinations, the majority of masses were mildly to moderately enhancing and heterogeneous, poorly marginated, and bronchocentric. Lymphadenopathy and pulmonary masses are the most common intrathoracic findings in dogs with histiocytic sarcoma, and the strong predilection for the ventral aspect of the right middle lung lube may help to differentiate it from other types of neoplasia.  相似文献   
46.
We provided comfortable resting areas in pens and tools for self-grooming and suppressing aggressive behavior for environmental enrichment in Japanese black and Japanese Shorthorn calves. At the start of the experiment, the black calves ( n  = 10) were 164 days old and 138 kg in weight, and the Japanese Shorthorn calves ( n  = 10) were 176 days old and 164 kg in weight. Calves of each breed were divided into two groups (the enrichment group and the non-treatment group), and each group was housed in a 4 m × 7.2 m pen. The effect of enrichment on calf welfare was estimated on the basis of daily weight gain and behavior. The brush stimulated the grooming behavior in calves although the mean time of using the brush decreased during the experiment. The partition of the feed trough and the wooden wall in the pen decreased the agonistic behavior in Japanese Shorthorn calves ( P  < 0.01) and increased the affiliative behavior in Japanese black calves ( P  = 0.08). The cleanliness of the bedding increased sternum lying and lying with the head touching the flank or ground in Japanese black calves and decreased the standing resting behavior in both breeds ( P  < 0.05 in all the cases). However, the treatments did not have an effect on daily weight gain. We concluded that the treatments would improve the welfare of calves and function as environmental enrichments.  相似文献   
47.
An experiment was conducted to determine the effects of inclusion levels of potato pulp silage in concentrate (0, 15, 30 or 45%; on a dry matter basis) and roughage sources (rice straw or wheat straw) on finishing performance and carcass quality of cull beef cows. Sixteen Japanese Black (Wagyu) mature cull cows (490 ± 31 kg of BW) were used in this experiment. Increasing the levels of potato pulp silage in concentrate and roughage sources did not significantly affect feed intake in cows. In addition, the final body weight, daily gain and feed : gain ratio were not influenced by the inclusion levels of potato pulp silage in concentrate and the type of roughage. Increasing the inclusion levels of potato pulp silage in concentrate would probably decrease the marbling score. The L* values of the longissimus muscle (LM) tended to respond quadratically ( P  = 0.078) as the inclusion levels of potato pulp silage in concentrate increased, and was lowest for cows fed the concentrate which included 30% potato pulp silage. The a* and b* values of the LM and fat color were not affected by the inclusion levels of potato pulp silage in concentrate. No effects of roughage sources on finishing performance were observed.  相似文献   
48.
To clarify colonic fermentation during the perinatal period, 22 dairy calves less than 6 weeks old were used. They were given a milk replacer following colostrum feeding. A total 100 samples of normal feces including meconium were collected from the rectum of the calves. Fecal pH, alcohols, lactate and volatile fatty acids (VFAs) were analyzed. Higher ethanol and n -propanol concentrations were found in many fecal samples particularly in the first 2 weeks after birth, but these metabolites showed consistently lower concentrations thereafter. By contrast, higher concentrations of methanol were observed in some samples for all ages examined. Fecal VFA increased abruptly within a few days of birth, and mainly consisted of acetate and n -butyrate. During the first 2 weeks, the proportion of n -butyrate in VFAs decreased and that of propionate increased gradually. Proportions of VFAs were almost stable at 3–6 weeks of age (acetate, propionate and n -butyrate in increasing order). Higher concentrations of lactate and lower pHs were observed in the fecal samples during the first 2 weeks, and concentrations decreased thereafter. Accelerated colonic production of ethanol and n -propanol was confirmed during the early 2 weeks, in addition to organic acid fermentation as reported previously.  相似文献   
49.
Isoenzyme variation of 198 isolates of Phytophthora infestans collected from many locations in Japan during 1987–90, and of four pre-1987 isolates, was examined using starch gel electrophoresis. A previously unreported allele at malic isoenzyme locus/ME (90) was observed. An association between mating types and isoenzyme genotypes at three isoenzyme loci, glucosephosphate isomerase (GPI-1), peptidase (PEP-1) and ME, was found. At PEP-1, the A2 isolates from Japan had a previously unreported genotype (96/96). Normal segregation at the malic isoenzyme locus occurred in a cross of Japanese and Mexican parents. The results provide evidence of a change in the population genetic structure of P. infestans in Japan.  相似文献   
50.
The effects of grazing and supplemental protein concentrations, provided during the grazing period, on subsequent finishing performance and carcass quality were investigated. This experiment was carried out using 15 Japanese Black cattle (Wagyu) steers. The steers were fed as follows: (i) grazing supplemented with moderate protein concentrate (GMP) (18% crude protein (CP); dry matter (DM) basis) before finishing, followed by feeding in a barn until slaughter; (ii) grazing supplemented with low protein concentrate (GLP) (14% CP; DM basis) before finishing, followed by feeding in the barn until slaughter; and (iii) no grazing before finishing (NG). From the end of the grazing season to the end of the growing period, the GMP and GLP steers were managed in the same way as the NG steers. All of the animals were fed the same diet in the finishing period (9 months to 28 months). None of the three treatments affected the average daily gains. For all treatments, chilled carcass weights were more than 450 kg and did not differ significantly among the groups. The longissimus muscle areas in the grazed steers were numerically larger than in those receiving the NG treatment. Fat thickness was significantly greater (P < 0.05) in the NG steers than in the grazed steers. The intramuscular fat and marbling scores were not affected in the grazed steers. In conclusion, the carcass quality of grazed steers was similar to that of the NG steers at a similar final age, and the quantity of meat in the NG steers would be less than that of the GMP and GLP steers. Therefore, spring‐born Wagyu steers should be grazed using the GLP supplement before fattening.  相似文献   
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