贵州魔芋软腐病菌多基因分子鉴定及其致病力分析     

黄露  周兵正  安星宇  王莉爽  姚令  陈小均  何海永  吴石平 《植物保护》2023,49(4):115-124

为明确贵州魔芋软腐病菌种类?致病力及分布特点, 采用组织分离法对贵州主要魔芋种植区软腐病样进行病原菌分离, 对icdA, mdh, mtlD, proA, rpoS 等5个管家基因进行了扩增?序列测定, 分别用单基因和多基因联合系统发育树对病菌进行鉴定, 同时采用组织块接种方法测定了不同菌株的致病力?通过组织分离法共分离魔芋软腐病菌株47株; 采用5个管家基因进行分子鉴定, 将病菌分别鉴定为海芋果胶杆菌Pectobacterium aroidearum?胡萝卜果胶杆菌Pectobacterium carotovorum和方中达迪基氏菌Dickeya fangzhongdai 3个种, 其中海芋果胶杆菌P.aroidearum为贵州魔芋软腐病主要致病菌, 占分离菌株的70%, 广泛分布在多个地区; 其次为方中达迪基氏菌D. fangzhongdai, 占分离菌株的28%, 也普遍存在于贵州各魔芋种植区; 胡萝卜果胶杆菌P. carotovorum最少, 占分离菌株的2%?致病力测定结果表明, 菌株间致病力存在一定的差异, 其中海芋果胶杆菌不同菌株之间致病力差异较大, 低?中?高致病力菌株都有, 方中达迪基氏菌差异较小, 仅有中?高致病力菌株?本研究确定了贵州魔芋软腐病菌种类?致病力及在贵州的分布特点, 首次报道了海芋果胶杆菌?方中达迪基氏菌是贵州魔芋软腐病的主要病原菌, 进一步加深了对魔芋软腐病及其发生流行的认识, 为软腐病的科学防控提供了科学依据?  相似文献   

玉米转Bt基因自交系的抗玉米螟特性鉴定初报   总被引：7，自引：0，他引：7  

刘宗华  汤继华  李宝健  范云六  李桂玲  季良越  陈伟程 《作物学报》2003,29(4):621-625

通过田间接卵和室内PCR分子检测,对采用回交法育成的Mo17Bt、478Bt、综3Bt和87-1Bt等4个转Bt基因自交系的抗虫性以及Bt基因的存在,进行鉴定.结果表明,4个转基因自交系群体的抗虫性明显提高,虫孔隧道长度比阴性对照平均缩短49.70%,差异达显著水平,但抗虫性不如阳性对照;群体内,单株间抗虫性仍存在差异,有高抗到高感不同类型,其  相似文献   

农民生计多样性影响因素调查分析 ——以丰都县为例     

金晓霞  郭岭  刘秀华 《中国农学通报》2007,23(8):619-619

丰都县农民生计多样性在整个西部地区比较具有代表性，但对于影响农民生计多样性的因素一直缺乏实证研究。该次调查采用参与性农村评估法（PRA），对丰都县名山镇（两汇口、古家店、鹿鸣寺）、社坛镇（三桥、德盛、干坛）、武平镇（殷家坝、坝周村）的农民生计方式及影响农民生计多样性的因素进行调查研究。研究表明，影响农民生计多样性的因素大致包括：自然资源、环境、人力资本、物质基础、社会关系、国家政策等方面  相似文献   

Regression Applied in Continuous Mathematical Forecast     

CAI Zhang-li  SHI Wei-ren  DIAO Ling 《保鲜与加工》2005,(10):91-93

Data Mining can distill the connotative, unknown and potential value information or pattern from many data. It is a problem that must be settled during the developing of Decision Support System how to discover knowledge from Data Warehouse. In order to realize the continuous mathematical forecast that will be used in the Marketing Decision Support System for Industrial Enterprise, the authors use the method of regression to discuss how to design the algorithm for Data Mining, how to design the model for Data Mining, and so on. When the algorithm applied to the sales forecast, the result processing to simulation data indicates it can realize the expected effect.  相似文献   

Communication System Design for Coal Mine Security Based on Nios II     

ZHANG Ling  DONG Lei  HE Wei 《保鲜与加工》2006,(11):89-91

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Design and Implement of WebGIS Based on SVG     

LIU Ling  LIU Shui-qing  WANG Yu 《保鲜与加工》2007,(4):108-112

SVG based on XML is a kind of language describing 2D vector graphics.It is usability and applicability to set up WebGIS.The paper puts forward a system structure of WebGIS based on SVG,which has been applied to campus electronic map system of Chongqing University.It has been proved the method is feasibility and practicability.The paper sets forth some key techniques of implement of the system in detail: coding method of organizing SVG spatial data;generation of SVG electronic map;storage of SVG spatial data and implement of client.  相似文献   

Reuolution and Research of Green Automobile     

RAN Zheng -y  TIAN Long  NI Ling  YANG Chao  CAO Wen -ming 《保鲜与加工》2002,(7):15-19

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.  相似文献   

A New Structure of Adaptive Decision Feedback Equalizer for FPGA Realization     

HE Wei  HAN Jian  ZHANG Ling 《保鲜与加工》2006,(12):104-106

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Vehicle Lateral Stability Control Based on Sliding Model Control     

ZHAO Shu-en  LI Yi-nong  ZHENG Ling  JI Jie 《保鲜与加工》2007,(8):1-5

In view of the vehicle cornering or path changing under ultimate handling maneuver, a 3-DOF nonlinear vehicle dynamics model which takes the vehicle lateral velocity, yaw rate and body roll angle as the State variables is presented. Based on the dynamic analysis, the method which using additional yaw moment produced by different longitudinal braking force among each wheels to improving vehicle handling stability in emergency situations is discussed. Considering the sideslip angle as one of the vehicle state variables is difficult to measuring, the sideslip angle estimation based on vehicle dynamic model and kinematics is designed. And then, based on the theory of siding model control, the combining sliding model control system is founded, which taking the vehicle yaw rate and body side-slip angle errors between the estimated and the real as the input variables, and taking the braking torque and the steering angle as control aims. The simulation results indicate that the control method can effectively improve the vehicle lateral handling stability.  相似文献   

Simulation Investigation for the Regulating Characteristics of Real and Reactive Power of Asynchronized Turbogenerators     

Ling Zhixians  Yang Shunchang  Qing Yihong  Xu Guoyu 《保鲜与加工》1994,(6):36-42

This paper discuas the controlling principle of asynchronized turbogenerators,deduces their mathematical model,and studies their regulating characteristics of real and reactive power(or voltage)by means of simulation calculation of operating characteristics for a 200MW asychronlzed turbogenerator.The conclusion is that the asychronlzed tubogenerators not only can regulate real and reactive power independently but also has an excellent dynamic performance.  相似文献