家蚕多肽抑制因子对酯酶A4的ATP酶活性影响   总被引：4，自引：1，他引：3  

徐世清  郑必平  陈息林  司马杨虎  虞晓华  甲斐英则  徐俊良 《蚕业科学》1999,25(1):16-21

从产下后２ｄ的家蚕Ｃ１０８品种滞育性卵精制酯酶Ａ４和其多肽抑制因子ＰＩＮ，体外调查了ＰＩＮ对酯酶Ａ４的ＡＴＰ酶活性影响。酯酶Ａ４和ＰＩＮ５℃混合２５～１３ｄ，除去ＰＩＮ后５℃保护，ＡＴＰ酶活性峰都在１２～１４ｄ后出现；２５℃混合时，除去ＰＩＮ后，ＡＴＰ酶活性峰出现时间为混合时间再加１２～１４ｄ。利用ＭＡＬＤＩＭＳ方法，首次检测到纯化的酯酶Ａ４和合成ＰＩＮ的结合体。  相似文献   

The cardiopulmonary effects of anesthetic induction with isoflurane,ketamine‐diazepam or propofol‐diazepam in the hypovolemic dog     

Sima Fayyaz DVM  BA  MSc  Carolyn L Kerr DVM  DVSc  PhD  Diplomate ACVA  Doris H Dyson DVM  DVSc  Diplomate ACVA  Kuldip K Mirakhur BVSc  MVSc  PhD 《Veterinary anaesthesia and analgesia》2009,36(2):110-123

ObjectiveTo evaluate and compare the cardiopulmonary effects of induction of anesthesia with isoflurane (Iso), ketamine–diazepam (KD), or propofol–diazepam (PD) in hypovolemic dogs.Study designProspective randomized cross–over trial.AnimalsSix healthy intact, mixed breed, female dogs weighing 20.7 ± 4.2 kg and aged 22 ± 2 months.MethodsDogs had 30 mL kg^?1 of blood removed at a rate of 1.5 mL kg^?1 minute^?1 under isoflurane anesthesia. Following a 30–minute recovery period, anesthesia was reinduced. Dogs were assigned to one of three treatments: isoflurane via facemask using 0.5% incremental increases in the delivered concentration every 30 seconds, 1.25 mg kg^?1 ketamine and 0.0625 mg kg^?1 diazepam intravenously (IV) with doses repeated every 30 seconds as required, and 2 mg kg^?1 propofol and 0.2 mg kg^?1 diazepam IV followed by 1 mg kg^?1 propofol increments IV every 30 seconds as required. Following endotracheal intubation all dogs received 1.7% end–tidal isoflurane in oxygen. Cardiopulmonary variables were recorded at baseline (before induction) and at 5 or 10 minute intervals following endotracheal intubation.ResultsInduction time was longer in Iso (4.98 ± 0.47 minutes) compared to KD (3.10 ± 0.47 minutes) or PD (3.22 ± 0.45 minutes). To produce anesthesia, KD received 4.9 ± 2.3 mg kg^?1 ketamine and 0.24 ± 0.1 mg kg^?1 diazepam, while PD received 2.2 ± 0.4 mg kg^?1 propofol and 0.2 mg kg^?1 diazepam. End–tidal isoflurane concentration immediately following intubation was 1.7 ± 0.4% in Iso. Arterial blood pressure and heart rate were significantly higher in KD and PD compared to Iso and in KD compared to PD. Arterial carbon dioxide partial pressure was significantly higher in PD compared to KD and Iso immediately after induction.Conclusions and clinical relevanceIn hypovolemic dogs, KD or PD, as used in this study to induce anesthesia, resulted in less hemodynamic depression compared to isoflurane.  相似文献   

Assessment of the oil content of the seed produced by <Emphasis Type="Italic">Salicornia</Emphasis> L., along with its ability to produce forage in saline soils     

Narges Reiahisamani  Mohammadali Esmaeili  Nayer Azam Khoshkholgh Sima  Faezeh Zaefarian  Mehrshad Zeinalabedini 《Genetic Resources and Crop Evolution》2018,65(7):1879-1891

Soil salinity presents a serious threat to crop production. The relatively poor tolerance to this stress agent exhibited by conventional crops incentivizes the search for alternative producers of food and forage in salinity-affected environments. Halophytes belonging to the genus Salicornia L. have been suggested as being able to provide both forage and edible seed oil. Here, a set of 14 Salicornia europaea L. accessions was investigated for their ability to produce seed oil and forage in response to a range of salt concentrations (100, 300 and 600 mM NaCl) in the water used for irrigation. Seed of the accessions was collected from diverse sites close to saline rivers and the sea in Iran. Salinity was shown to have a major effect on biomass yield, and on seed oil yield and composition. The ratio of unsaturated to saturated fatty acids in the seed oil was remarkably high. Forage yield was highest when irrigated with 300 mM NaCl for most of the accessions, while a level of 600 mM NaCl suited the production of seed oil.  相似文献   

Application of secondary-treated effluents for cultivation of sunflower (Helianthus annuus L.) and celosia (Celosia argentea L.) as cut flowers     

Haya Friedman  Nirit Bernstein  Moshe Bruner  Ilona Rot  Zeev Ben-Noon  Atara Zuriel  Ron Zuriel  Sima Finkelstein  Nakdimon Umiel  Amir Hagiladi 《Scientia Horticulturae》2007

In this study we have examined the effects of irrigation with municipal secondary-treated effluents on growth of sunflower (Helianthus annuus L.) and celosia (Celosia argentea var cristata L.) plants for cut flower production. The applied treated effluents contained higher levels of Na (∼X6), Cl (∼X4.5), N–NH₄ (30–50 mg/l), B, HCO₃, P, K, and Fe than the potable water. The effect of the two types of irrigation water on the chemical composition of soil and leaves, flower yield and postharvest performance was examined. Our results demonstrate higher accumulation of Cl, Na, B, and NO₃ in soils irrigated with effluents as compared to potable water only in the 40–60 cm soil layer. Phosphorus accumulated to higher levels in the upper soil layer (0–20 below ground), as well as in the lower soil layer, irrigated with effluents. SAR levels as well, were higher under irrigation with the treated effluents in the soil until 40 cm below ground indicating higher Na absorption in comparison to Ca and Mg. In both sunflower and celosia, boron accumulated to higher levels in leaves of plants irrigated with effluents. In addition, under irrigation with effluents, celosia accumulated higher levels of N and Mn and sunflower higher levels of P and lower levels of Fe. Despite these differences in mineral contents, the quality of the irrigation water did not affect cut flower yield. In both species spike length, receptacle size, number of flowers and flower weight were not affected. However, flowers’ quality, examined during vase life, was compromised by irrigation with the effluents. Celosia leaves appeared more yellow and sunflower petal edges became browner. Nevertheless, leaf yellowing in celosia occurred mainly on the lower leaves of the stem, which can be removed to maintain high commercial value. The reduction in sunflower quality as well, will most likely not pose any commercial problem since these negative symptoms appeared at a late stage of vase life. Overall, our results demonstrate that secondary-treated municipal effluents can be used for production of sunflower and celosia for cut flowers, in the northern-eastern part of the ‘Negev’ desert in Israel. Due to the high SAR values of the water, and in order to avoid damage to the soil structure, dilution of this water should be considered for commercial growth.  相似文献   

Summaries of lectures presented at a binational UK—Israel conference agriculture in a cleaner environment — new technologies from Britain and Israel     

J. A. Pickett  G. M. Tatchell  D. M. Glen  B. Raccah  Sima Singer  D. Veierov  Y. Spiegel  I. Chet  D. Shtienberg  U. Mingelgrin  J. Hart 《Phytoparasitica》1995,23(4):359-366

Extended summaries and abstracts of the lectures on pest and disease control, and on reduction of pesticidal pollution in the environment, presented within the framework of a conference which was organized by the Biotechnology & Biological Sciences Research Council (BBSRC), Swindon, UK, and the Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, Israel.  相似文献   

用不同分析方法评价家蚕品种锦·绣×潇·湘的丰产性和稳产性及丝质性状     

艾均文  司马杨虎  何行健  薛宏  唐芸  郑颖  刘勇 《蚕业科学》2019,45(1):54-60

对新选育品种进行全面而合理的评判是家蚕品种选育工作的一个重要环节。根据2015—2017年家蚕品种国家审定多点鉴定成绩的年度汇总数据,利用非参数统计法、高稳系数法以及稳定性参数法等分析方法,分别对农村生产鉴定成绩中的盒种产茧量、健蛹率与实验室鉴定成绩中的茧丝长、解舒率与洁净等反映家蚕品种高产稳产、丝质性状的关键性指标进行水平比较和稳定性分析,其结果与平均数、标准差、变异系数等常用方法的分析结果趋向相一致。家蚕品种锦·绣×潇·湘的盒种产茧量比对照品种秋丰×白玉高12.07%,表现出了产茧量高、稳产性强、丝质优的特点,具有广阔的推广应用前景。  相似文献   

4H12, a Murine Monoclonal Antibody Directed against Myosin Heavy Chain-9 Expressed on Acinar Cell Carcinoma of Pancreas with Potential Therapeutic Application     

Sima Balouchi-Anaraki  Simin Ahmadvand  Akbar Safaei  Abbas Ghaderi 《Iranian Biomedical Journal》2021,25(5):310

Background:PACC is a rare type of pancreatic exocrine neoplasm that is frequently diagnosed at late stages with a high rate of metastasis. Identification of new biomarkers for PACC can improve our knowledge of its biology, early detection, or targeted therapy. In this study, hybridoma technology was used to generate mAbs against Faraz-ICR, a pancreatic acinar cell carcinoma cell line. Methods: Cell ELISA and flow cytometry were used for screening, and the 4H12 hybridoma clone was selected for further analysis. The 4H12 mAb was specific for MYH9 as determined by Immunoprecipitation, Western blot, and mass spectrometry. Results:This antibody reacted variably with other cancer cells, in comparison to Faraz-ICR cell. Besides, by immunohistochemical staining, the acinar cell tumor, which was the source of Faraz-ICR, showed high MYH9 expression. Among 21 PDAC cases, nine (42.8%) expressed MYH9 with low intensity, while 10 (47.8%) and 2 (9.5%) cases expressed MYH9 with moderate to strong intensities, respectively. The 4H12 mAb inhibited the proliferation of Faraz-ICR cells in a dose-dependent manner from 0.75 to 12.5 μg/ml concentrations (p < 0.0001 and p < 0.002). IC₅₀ values were achieved at 12.09 ± 4.19 µg/ml and 7.74 ± 4.28 µg/ml after 24- and 48-h treatment, respectively. Conclusion:Our data suggest that the 4H12 mAb can serve as a tool for investigating the role of MYH9 pancreatic cancer biology and prognosis. Key Words: Acinar cell carcinoma, Biomarkers, Monoclonal antibody, Pancreas  相似文献   

Viral disease of tomato crops (Solanum lycopesicum L.): an overview     

Ong  Sheue Ni  Taheri  Sima  Othman  Rofina Yasmin  Teo  Chee How 《植物病害和植物保护杂志》2020,127(6):725-739

Journal of Plant Diseases and Protection - Global production of tomato has been hampered by the increased incidences of tomato viral disease. The high genetic heterogeneity of tomato plant viruses,...  相似文献   

PHYSIOLOGICAL RESPONSE OF SPRING DURUM WHEAT GENOTYPES TO SALINITY     

Kambiz Azizpour  M. R. Shakiba  N. A. Khosh Kholg Sima  H. Alyari  M. Mogaddam  E. Esfandiari 《Journal of plant nutrition》2013,36(6):859-873

Soil salinity is a serious threat in many parts of Iran, which negatively affects plant production. In order to investigate response of durum wheat to salinity, two genotypes, ‘Turkey 506’ (salt tolerant) and ‘Egypt 557’ (salt sensitive), were grown in hydroponic conditions, exposed to various salt levels (0, 50, 100, 150 and 200 mmol NaCl) in a split split plot based on randomized complete block design with three replications of each treatment. Salinity stress decreased relative water content (RWC), potassium content, potassium/sodium ratio, chlorophyll a (chla), chlorophyll b (chlb), and total chlorophyll contents, efficiency of photosystem II (F_v/F_m) and membrane stability index (MSI), and increased sodium, proline and soluble sugars concentrations and ratio of chla/chlb in both genotypes. The decrease in RWC, chla, chlb, F_v/F_m, and MSI were significantly higher in ‘Egypt 557’ than ‘Turkey 506’. ‘Turkey 506’ showed higher content of potassium, potassium/sodium ratio, proline, and soluble sugar concentrations as well as lower sodium content as compared with ‘Egypt 557’. The salinity tolerance of ‘Turkey 506’ is associated with higher RWC, potassium content, osmolyte concentrations, chlorophyll contents, F_v/F_m ratio, and maybe more vacuole sequestration of sodium.  相似文献   

Coagulation profile,haematological and biochemical changes in kids naturally infected with peste des petits ruminants     

Sahinduran S  Albay MK  Sezer K  Ozmen O  Mamak N  Haligur M  Karakurum C  Yildiz R 《Tropical animal health and production》2012,44(3):453-457

The aim of this study was to examine the coagulation profile in peste des petits ruminant (PPR) in kids. Five kids from a group of 150 animals (72 goats and 78 kids) were brought to the Veterinary Medical Teaching Hospital from a farm in Burdur province (Turkey) with nasal and ocular discharges and diarrhea. Fifteen goats and 41 kids had died due to diarrhea and three kids were presented to the Department of Pathology for diagnosis. Blood samples were taken from 12 ill animals (infected group) for haematological and biochemical analysis. In addition, five healthy kids were examined from another healthy flock (control group). Leukocyte and lymphocyte numbers of infected group showed significant declinations in comparison to control group (≤0.001). Haemorrhages in all organs of digestive system and small haemorrhagic areas in liver were caused to decrease in erythrocyte and haematocrit values (p ≤ 0.001) in infected group. Concentrations of blood urea nitrogen (BUN) (p ≤ 0.01) and creatinine (p ≤ 0.001) in infected group were significantly higher than control group. Compared to control group, significant increases were determined in serum concentrations of alkaline phosphatase (ALP) (p ≤ 0.01), aspartate aminotransferase (AST) (p ≤ 0.001) and alanine aminotransferase (ALT) (p ≤ 0.001) in the infected group. No significant differences were observed between the infected and control groups for serum gamma glutamyl-transferase (GGT) concentration value. In our study, thrombocytopenia (p ≤ 0.001) together with prolonged activated partial thromboplastin time (APTT; p ≤ 0.01) and prothrombin time (PT; p ≤ 0.001) may show that disseminated intravascular coagulopathy which can occur in kids with PPR.  相似文献