Rice Germin-Like Proteins: Allelic Diversity and Relationships to Early Stress Responses   总被引：1，自引：0，他引：1  

Rebecca M. Davidson  Patricia M. Manosalva  Jacob Snelling  Myron Bruce  Hei Leung  Jan E. Leach 《Rice》2010,3(1):43-55

Germin-like protein (GLP) markers were associated with quantitative trait loci (QTL) for resistance to the rice blast pathogen, Magnaporthe oryzae in multiple rice (Oryza sativa) mapping populations. Twelve paralogous OsGLP gene family members are located within the physical QTL region on chromosome 8, and gene silencing studies suggest that they contribute collectively to the resistance phenotype. We compared sequence and expression profiles of OsGLP alleles in two resistant and two susceptible parental rice lines to find functional polymorphisms that correlated with the resistant phenotype. Based on coding and promoter sequences, the genes belong to two germin subfamily groups (GER3 and GER4). OsGLP members from both subfamilies were constitutively expressed and developmentally regulated in all cultivars. Transient induction above constitutive levels was observed for some OsGLPs, especially GER4 subfamily members, at early time points after M. oryzae infection and mechanical wounding. Varying 5′ regulatory regions and differential expression of some family members between resistant and susceptible cultivars corresponded with differential hydrogen peroxide (H₂O₂) accumulation after the same stimuli. OsGLP of both GER subfamilies localized to the plant cell wall. The protein location and early gene induction suggest that OsGLPs protect rice leaves at early stages of infection before fungal penetration and subsequent ingress. Our data suggest that regulation of OsGLP genes defines resistant versus susceptible phenotypes.  相似文献   

A review of cases submitted to Murdoch University for immunodiagnostic testing: 1978–1989     

WJ PENHALE  MJ DAY†  AD LINES  RP McKENNA 《Australian veterinary journal》1990,67(4):148-149

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Vasoformative proliferations in a rufous bettong     

P CANFIELD  T BELLAMY  WJ HARTLEY  DS SPIELMAN 《Australian veterinary journal》1990,67(9):334-335

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Current therapy in equine medicine 3     

WJ 《Journal of Equine Veterinary Science》1992,12(4)

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Farm management factors associated with bulk tank total bacterial count in Irish dairy herds during 2006/07     

Kelly P  O'Sullivan K  Berry D  More S  Meaney W  O'Callaghan E  O'Brien B 《Irish veterinary journal》2009,62(1):36-42

Research has shown that total bacterial count (TBC), which is the bacterial growth per ml of milk over a fixed period of time, can be decreased by good hygiene and farm management practices. The objective of the current study was to quantify the associations between herd management factors and bulk tank TBC in Irish spring calving, grass-based dairy herds. The relationship between bulk tank TBC and farm management and infrastructure was examined using data from 400 randomly selected Irish dairy farms where the basal diet was grazed grass. Herd management factors associated with bulk tank TBC were identified using linear models with herd annual total bacterial score (i.e., arithmetic mean of the natural logarithm of bulk tank TBC) included as the dependent variable. All herd management factors were individually analysed in a separate regression model, that included an adjustment for geographical location of the farm. A multiple stepwise regression model was subsequently developed. Median bulk tank TBC for the sample herds was 18,483 cells/ml ranging from 10,441 to 130,458 cells/ml. Results from the multivariate analysis indicated that the following management practices were associated with low TBC; use of heated water in the milking parlour; participation in a milk recording scheme; and tail clipping of cows at a frequency greater than once per year. Increased level of hygiene of the parlour and cubicles were also associated with lower TBC. Herd management factors associated with bulk tank TBC in Irish grazing herds were generally in agreement with most previous studies from confinement systems of milk production.  相似文献   

Radiographic changes in elbow dysplasia following ulnar osteotomy--a case report and review of the literature     

Snelling SR  Lavelle RB 《Australian veterinary journal》2004,82(5):278-281

This case report describes the results of bilateral elbow arthrotomy and a unilateral ulnar osteotomy in a 10-month-old dog with bilateral elbow dysplasia. Fragmented coronoid processes were removed from both joints via bilateral medial arthrotomies. On the right side an ulnar osteotomy was also performed using a caudolateral approach. Bilateral flexed lateral elbow radiographs taken before surgery and 5 months postoperatively were assessed for changes in osteophyte size on the anconeal process, caudal epicondylar ridge and radial head. Osteophytes on the anconeal process and caudal epicondylar ridge disappeared over the postoperative period in the joint that received an ulnar osteotomy. In the contralateral joint the size of the oesteophytes on the anconeal process and caudal epicondylar ridge increased in size during the same period.  相似文献   

A retrospective study of unilateral arytenoid lateralisation in the treatment of laryngeal paralysis in 100 dogs (1992-2000)     

Snelling SR  Edwards GA 《Australian veterinary journal》2003,81(8):464-468

OBJECTIVE: To determine the effectiveness of unilateral arytenoid lateralisation as a surgical treatment for laryngeal paralysis in dogs. DESIGN: The case records of 100 dogs that received a unilateral arytenoid lateralisation for laryngeal paralysis between 1992 and 2000 were reviewed. The results of questionnaires on surgical outcome, formulated for the animal owner and the referring veterinarian, were analysed. Information obtained for dogs under 10 kg and dogs over 10 kg was analysed separately. RESULTS: The Labrador Retriever was the most commonly affected breed. The male:female ratio was 1.56:1 and the average age of presentation was 9.9 years. The most common month in which surgery was performed was October. The majority of owners (87.7%) felt that their dog's quality of life was improved in the 6 months after surgery. Thirty-three percent of dogs revisited the referring clinic with a respiratory problem following unilateral arytenoid lateralisation, and 10.7% of dogs were reported as having a post-surgical complication associated with the procedure. Following surgery, dogs under 10 kg revisited the referring veterinarian with a respiratory complication more often than dogs over 10 kg. Significantly fewer owners of dogs under 10 kg than owners of dogs over 10 kg felt that their dogs quality of life was improved by surgery (55% versus 93%). CONCLUSION: The majority of owners surveyed reported that unilateral arytenoid lateralisation had improved the quality of their dog's life during the first 6 postoperative months. Owner dissatisfaction with the results of surgery and the reported rate of re-presentation (for respiratory disease) may be higher for small (< 10 kg) dogs. A prospective study comparing the results of unilateral arytenoid lateralisation surgery in large and small dogs may be worthwhile in the future.  相似文献   

Fine mapping of quantitative trait loci and assessment of positional candidate genes for backfat on bovine chromosome 14 in a commercial line of Bos taurus   总被引：6，自引：0，他引：6  

Moore SS  Li C  Basarab J  Snelling WM  Kneeland J  Murdoch B  Hansen C  Benkel B 《Journal of animal science》2003,81(8):1919-1925

Backfat thickness is one of the major quantitative traits that affect carcass quality in beef cattle. In this study, we have fine mapped a QTL for backfat EBV on bovine chromosome 14, using an identical-by-descent haplotype-sharing analysis, in a commercial line of Bos taurus. We also examined the association between gene-specific single nucleotide polymorphism (SNP) markers of the genes diacylgcerol acyltransferase 1 (DGAT1) and thyroglobulin (TG) and the backfat EBV. The results indicate that the QTL region for backfat identified on chromosome 14 is in agreement with previous studies. However, neither of the two polymorphisms of candidate genes tested, DGAT1 nor TG, showed a significant (P > 0.10) association with the backfat EBV in the cattle populations examined. However, a strong association (P = 0.0058) was detected between a microsatellite marker (CSSM66) lying approximately mid-way between the two candidate genes and the backfat EBV. These results suggest that other SNP of DGAT1, TG, or other gene(s) in the chromosomal region should be examined to test whether they have a significant effect on lipid metabolism.  相似文献   

Evidence of dehydration and electrolyte disturbances in cases of perennial ryegrass toxicosis in Australian sheep     

MDA Combs  D Rendell  KFM Reed  WJ Mace  JC Quinn 《Australian veterinary journal》2014,92(4):107-113

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Ventral fixation of chronic atlanto-occipital luxation in a dog     

Buks Y  Snelling SR  Yates GD 《The Journal of small animal practice》2011,52(9):497-500

A 4·5-year-old male Australian Kelpie was presented for evaluation of ambulatory tetraparesis and neck pain of five weeks duration. Atlanto-occipital luxation was diagnosed by computed tomography. The joint was unstable following closed reduction, and a ventral surgical approach to the cranial cervical spine and occiput permitted manual reduction and stabilisation of the atlanto-occipital joint. The thickened joint capsule and articular cartilage were removed to promote AO arthrodesis. Eight cortical screws, inserted into the occipital condyles and C1, were embedded in polymethylmethacrylate to stabilise the atlanto-occipital articulation. The dog recovered uneventfully after it was placed in a neck brace and rested for six weeks. Neurologic examination at six weeks was normal and the dog returned to normal farm work 12 weeks after surgery.  相似文献