Inoculation of neonatal gnotobiotic dogs with a canine rotavirus     

C A Johnson  R W Fulton  W G Henk  T G Snider 《American journal of veterinary research》1983,44(9):1682-1686

Neonatal gnotobiotic dogs were inoculated orally with a rotavirus isolated from a pup with fatal diarrhea, and in the gnotobiotic dogs, diarrhea was observed between postinoculation hours (PIH) 20 and 24. The diarrhea persisted through PIH 154, and inoculated pups had clinical signs of dehydration after PIH 24. Negative-contrast electron microscopy of the feces from inoculated pups revealed rotavirus particles from PIH 12 through 154. Using an indirect-fluorescent antibody test, serum rotavirus antibody was detected in inoculated pups by PIH 96. In the duodenum, jejunum, and ileum of inoculated pups, group-specific rotaviral antigen was observed within absorptive villus epithelial cells and mononuclear cells in the villus lamina propria with an indirect-fluorescent antibody test. Fluorescence was seen in the small intestine of inoculated pups killed by PIH 12 and was present in intestines of pups killed through PIH 154. Rotaviral antigen was also seen in the mesenteric lymph nodes of a few inoculated pups killed at PIH 48.  相似文献   

Pharmacokinetics of bromide in adult sheep following oral and intravenous administration          下载免费PDF全文

TA Quast  MD Combs  SH Edwards 《Australian veterinary journal》2015,93(1-2):20-25

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日粮中添加不同锰、锌源对肉鸡生长性能及免疫机能的影响     

张海琴  闫素梅  塔娜  丁晓岚  乔良  康越 《内蒙古农业大学学报(自然科学版)》2006,27(2):17-20

将216只1日龄健康艾维茵肉鸡随机分为4个处理组,试验期7周。处理组日粮分别在锰含量为37.66mg/kg、锌含量为21.87mg/kg的基础日粮中添加不同的锰源与不同的锌源,其中处理1组为无机锰 无机锌,处理2组为有机锰 无机锌,处理3组为无机锰 有机锰 无机锌,处理4组为无机锰 有机锰 无机锌 有机锌,研究不同锰、锌源对肉鸡生长性能及免疫机能的影响。结果表明,氨基酸螯合锰部分或全部替代日粮中的无机锰,肉鸡生长速度及饲料转化效率有增加的趋势,氨基酸螯合锰、锌同时替代无机锰、锌,肉鸡的生长速度显著增加(p<0.05),饲料转化率也有增加的趋势。氨基酸螯合锰、锌与无机锰、锌相比,有促进机体免疫机能的趋势。  相似文献   

工厂化水产养殖水质预测预警系统的设计     

他旭翔  肖瑞超  张龙  位耀光 《安徽农业科学》2017,45(22)

在对养殖水质需求分析的基础上,设计了一种水质在线实时预测预警系统。该系统具有以下功能:水质实时信息和预测信息的查询、水质预警、水质预警设置和用户管理。在多媒体可视化界面的引导下,信息操作人员可以根据养殖场的实际情况,进行各种预警指标的设定和预警方式的设置。该系统较好地解决了工厂化养殖过程中水质监测预警问题,方便了用户对养殖用水水质的监控和管理。  相似文献   

Ultrasound Guided Transplantation of Enriched and Cryopreserved Spermatogonial Cell Suspension in Goats     

G Kaul  J Kaur  TA Rafeeqi 《Reproduction in domestic animals》2010,45(6):e249-e254

Spermatogonial stem cells transplantation provides a unique approach for studying spermatogenesis. Initially developed in mice, this technique has now been extended in farm animals and provides an alternative means to preserve valuable male germ line and to produce transgenic animals. The aim of this study was to enrich type A spermatogonial cells amongst the isolated cells from goat testis, to cryopreserve these enriched populations of cells and their subsequent transplantation in unrelated recipient goats under ultrasound guidance. The cells were isolated enzymatically and enriched by differential plating and separation on discontinuous percoll gradient. Ultrasound guided injection of trypan blue dye into rete testis resulted in 20–30% filling of the seminiferous tubules. Prior to transplantation, the cells were labelled with a fluorescent dye to trace donor cells in recipient seminiferous tubules after transplantation. The fluorescent‐labelled cells were observed up to 12 weeks after transplantation.  相似文献   

皮、裸燕麦种质资源的性状表现和遗传差异分析   总被引：1，自引：0，他引：1  

塔娜  逯晓萍  张雅慧  张晓宇  薛春雷  赵卫国 《内蒙古农业科技》2010,(2):33-35,41

研究利用4份皮燕麦和5份裸燕麦为材料,对9份燕麦材料的产量性状和抗性进行了分析,结果表明:皮燕麦的产量低于裸燕麦的产量,而抗性表现是皮燕麦的抗性比裸燕麦的抗性强,特别是抗倒伏能力尤为突出。同时,采用RAPD分子标记技术,从DNA水平对9份燕麦种质的遗传差异性进行了研究。结果显示:从50条RAPD引物中筛选出6条多态性较强的引物,共扩增出DNA片段68条,其中具多态性带33条,多态率为48.5%,每引物平均多态位点5.5个。裸燕麦与皮燕麦的遗传距离较大,为0.087～0.496,而皮燕麦与皮燕麦以及裸燕麦与裸燕麦之间的遗传距离较小,分别为0.023～0.117和0.040～0.298。采用非加权类平均法(UPGAM)进行了聚类分析,当遗传距离为0.26时,将供试材料划分为3个类群,并与实际结果一致。  相似文献   

Statistical evaluation of transcriptomic data generated using the Affymetrix one-cycle, two-cycle and IVT-Express RNA labelling protocols with the Arabidopsis ATH1 microarray     

Tara J Holman  Michael H Wilson  Kim Kenobi  Ian L Dryden  Charlie T Hodgman  Andrew TA Wood  Michael J Holdsworth 《Plant methods》2010,6(1):9

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云南省猪布鲁氏杆菌病血清学调查     

杨渊  他月芬  杨红华  牛木存  杨贵树 《现代农业科技》2011,(4):319+324

采用虎红平板凝集试验与试管凝集试验相结合的方法,对云南省部分养殖场和屠宰厂采集的猪血清样本进行布病血清学检测,检测血清样本302份。经虎红平板凝集试验筛选,共检出阳性血清样本30例,检出率为9.934%。阳性者通过试管凝集试验证实,最终检出阳性血清样本14例,检出率为4.636%。其中屠宰厂检出率为6.091%,养殖场检出率为1.905%,屠宰场的检出率高于养殖场。  相似文献   

Diversity and abundance of earthworms across an agricultural land-use intensity gradient     

Richard G. Smith  Claire P. McSwiney  A. Stuart Grandy  Pongthep Suwanwaree  Renate M. Snider  G. Philip Robertson 《Soil & Tillage Research》2008,100(1-2):83-88

Understanding how communities of important soil invertebrates vary with land use may lead to the development of more sustainable land-use strategies. We assessed the abundance and species composition of earthworm communities across six replicated long-term experimental ecosystems that span a gradient in agricultural land-use intensity. The experimental systems include a conventional row-crop agricultural system, two lower-intensity row-crop systems (no-till and tilled organic input), an early successional old-field system, a 40–60 years old coniferous forest plantation, and an old-growth deciduous forest system. Earthworm populations varied among systems; they were lowest in the most intensively managed row-crop system (107 m⁻²) and coniferous forest (160 m⁻²); intermediate in the old-field (273 m⁻²), no-till (328 m⁻²) and tilled organic (344 m⁻²) cropping systems; and highest in the old-growth deciduous forest system (701 m⁻²). Juvenile Aporrectodea species were the most common earthworms encountered in intensively managed systems; other species made up a larger proportion of the community in less intensively managed systems. Earthworm community biomass and species richness also varied and were lowest in the conventional row-crop system and greatest in the old-growth forest system. These results suggest that both land-use intensity and land-use type are strong drivers of the abundance and composition of earthworm communities in agricultural ecosystems.  相似文献   

人CD8~+T细胞基因调控网络研究     

韩鹏勇  塔娜  宝鲁日  于海泉 《西北农业学报》2019,28(6):861-867

为研究CD8~+ T细胞的功能异常机制,对功能异常状态CD8~+ T细胞和3种正常CD8~+ T细胞亚型[中央记忆CD8~+ T细胞(HCM)、效应记忆CD8~+ T细胞(HEM)和na?ve CD8~+ T细胞(HW)]的ATAC-seq(Assay for transposase accessible chromatin with high-throughput sequencing)数据进行基因调控网络比较,结果表明,3种正常CD8~+ T细胞特异表达的基因参与维持相应T细胞正常功能的信号通路,功能异常CD8~+ T细胞特异的基因参与癌症相关通路。转录因子调控网络比较分析发现功能异常CD8~+ T细胞GMEB2转录因子表达关闭。细胞表面受体调控网络比较分析发现功能异常CD8~+ T细胞CD7和P4HB受体的表达关闭。采用基因调控网络的数据进行比较分析,发现CD8~+ T细胞功能异常机制可能与GMEB2转录因子、CD7和P4HB受体表达关闭有关,这为抗病育种寻找特定基因提供新思路。  相似文献