Prevalence of gastric ulcers in Thoroughbred broodmares in pasture: a preliminary report     

le Jeune SS  Nieto JE  Dechant JE  Snyder JR 《Veterinary journal (London, England : 1997)》2009,181(3):251-255

Gastroscopic examinations were performed in 62 Thoroughbred broodmares (33 pregnant, 29 non-pregnant) at one breeding farm to investigate the prevalence of gastric ulceration. Age, pregnancy status, race earnings, last race start, herd size, medical history, number of live foals, breeding years, feed type and number of feedings were recorded, plus coat condition and body condition score were determined. Twenty-one mares were re-evaluated after foaling, and the foaling date, foal weight at birth and placenta weight were recorded.The overall prevalence of gastric ulcers was 70.9%, with a median ulcer score of 3.0 (range: 2–5). Most ulcers were present on the squamous portion of the stomach, while two mares had glandular ulcers. There were no differences in the presence, location and severity of gastric ulcers between pregnant and non-pregnant mares. Furthermore, there were no significant associations between the variables measured and the presence of gastric ulceration. The prevalence of gastric ulceration in this specific population of horses was higher than expected and further investigation is warranted to determine the factors that contributed to this finding.  相似文献   

云南景东县豆科固氮树种及根瘤菌资源调查   总被引：1，自引：0，他引：1  

贾风勤  杨比伦  腊萍 《西南林学院学报》2009,29(4):13-18

对云南景东县豆科固氮树种进行调查采集与鉴定,确认并记录70种与相应的根瘤菌(菌株)共生的豆科固氮树种.其中,云实亚科1属1种,含羞草亚科6属20种,蝶形花亚科23属49种.根瘤大小不一,形状各异,有球形、椭圆形、棒状、珊瑚状;表面颜色有浅黄色、棕黑色、棕色或黑色.对以活体保存的材料进行根瘤菌的分离、鉴定,获得并保存了分别与其中38种豆科树种相共生的根瘤菌菌株147株.调查表明,在景东县及云南南亚热带区域和热带区域,不仅拥有可供在多方面开发利用的豆科固氮树种,且具有丰富的可供科研和开发利用的根瘤菌资源.  相似文献   

甜菜SSR反应体系优化及重要农艺性状分子标记   总被引：5，自引：0，他引：5  

代培红  腊萍  郭长奎  高文伟  章建新  罗淑萍 《新疆农业科学》2013,50(7):1199

[目的]为选育优质甜菜新品种,利用SSR法筛选与高糖、高产、耐盐相关的分子标注.[方法]研究对甜菜SSR-PCR反应体系进行优化,并利用SSR分子标记方法和分离群体分组分析法(Bulked SegregateAnalysis,BSA)对具有高糖/低糖、低产/高产、耐盐/不耐盐三种重要农艺性状的甜菜亲本和F2代植株进行分析.[结果]研究建立了适宜甜菜的SSR-PCR反应体系为:20μL反应体系中含l×Buffer、2.0 mmol/LMg2+、1.5 uTaq DNA聚合酶、0.20 mmol/L dNTP、1.5 μmol/L引物、60 ng DNA模板.依据优化体系,对不同农艺性状的甜菜亲本与F2代进行SSR-PCR扩增分析,高糖性状获得了200和100 bp两条与高糖性状连锁的标记,250和230 bp两条与高产性状连锁的标记,550、250和100 bp三条与耐盐性状紧密连锁的分子标记.[结论]研究获得的7条特异条带是与甜菜重要农艺性状连锁的分子标记,将为甜菜的育种工作提供重要的理论基础.  相似文献   

Estimation of actual evapotranspiration in winegrape vineyards located on hillside terrain using surface renewal analysis     

T. M. Shapland  R. L. Snyder  D. R. Smart  L. E. Williams 《Irrigation Science》2012,30(6):471-484

Sensible and latent heat flux densities were estimated in a level vineyard, a northeast aspect vineyard and a southwest aspect vineyard in the Napa Valley of California using the eddy covariance and surface renewal methods. Surface renewal is theoretically not limited to level or extensively homogeneous terrain because it examines a more localized process of scalar exchange as compared with eddy covariance. Surface renewal estimates must be calibrated against eddy covariance data to account for unequal heating of the air parcels under a fixed measurement height. We calibrated surface renewal data against eddy covariance data in a level vineyard, and the calibration factor (α) was applied to the surface renewal measurements on the hillside vineyards. Latent heat flux density was estimated from the residual of the energy balance. In the level vineyard, the average daily actual evapotranspiration (ET _a ) for the period of June through September was 2.4?mm per day. In the northeast aspect vineyard, the average daily ET _a was 2.2?mm per day, while in the southwest aspect vineyard it was 2.7?mm per day. The net radiation values for the level vineyard, the northeast aspect vineyard, and the southwest aspect vineyard were compared against the Ecosystem Water Program with good agreement.  相似文献   

Juice, Pulp and Seeds Fractionated from Dry Climate Primocane Raspberry Cultivars (Rubus idaeus) Have Significantly Different Antioxidant Capacity, Anthocyanin Content and Color     

Shannon M. Snyder  Richard M. Low  Janet C. Stocks  Dennis L. Eggett  Tory L. Parker 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(4):358-364

Raspberries contain flavonoid antioxidants whose relative concentrations may vary between the juice, pulp, and seed fractions. Oxygen radical absorbance capacity (ORAC), total anthocyanin content, and berry color were determined for six cultivars of primocane raspberries grown in a dry climate (Utah, USA). Significant ORAC differences were found between juice (18.4?±?0.39 μmol TE/g), pulp (24.45?±?0.43), and seeds (273.27?±?11.15) with all Utah cultivars combined. A significantly higher concentration of anthocyanins was present in Utah raspberry juice (20.86?±?0.35 mg cyanidin-3-glucoside eq./100 g), compared to pulp (13.96?±?0.35). Anthocyanin content of juice and pulp were significantly positively correlated with dark color (L*). This is the first report of fractional differences in dry climate raspberries, and has implications for the juice and supplement industries.  相似文献   

毕节地区植烟土壤肥力状况评价   总被引：3，自引：1，他引：2  

代昌明  腊贵晓  翟欣  陈文相  陈雪  喻奇伟  刘国顺 《江西农业学报》2011,23(8):9-11,16

2007～2008年对在毕节地区主要烟区采集的200个植烟土壤样品进行了土壤养分含量分析,结果表明:植烟土壤的有机质含量平均在27.70 g/kg,总体上处于适宜水平;pH值以偏酸性或中性为主;有效氮含量偏高;速效磷含量偏低;速效钾含量整体丰富,平均为158.42 mg/kg;土壤水溶性氯含量整体适宜。此外,针对土壤养分含量状况,提出了一些相应的改良措施。  相似文献   

Effects of thermal acclimation on tissue fatty acid composition of freshwater alewives (Alosa pseudoharengus)     

Snyder RJ  Schregel WD  Wei Y 《Fish physiology and biochemistry》2012,38(2):363-373

In this study, we examine changes in fatty acid composition of polar and neutral lipids in gill, liver, and muscle of freshwater alewives (Alosa pseudoharengus) after temperature challenges in the laboratory. Alewives experienced either a warm or cold challenge in which temperatures were raised or lowered 0.5°C day⁻¹ over 4–6 weeks. In alewives experiencing the cold challenge, gill polar lipids showed evidence of significant remodeling, including decreases in palmitic acid and saturated fatty acids and increases in n-3 and n-6 highly unsaturated fatty acids including docosahexaenoic acid (DHA) and arachidonic acid. In alewives experiencing the warm challenge, we observed significant increases in saturated fatty acids (due mainly to increased palmitic acid) and decreases in polyunsaturated fatty acids in polar lipids of muscle and liver tissue. Fish that died during the cold challenge had significantly higher levels of palmitic acid in muscle polar lipids compared to fish that survived; fish that died during the warm challenge displayed complex changes in fatty acid composition. Based on theoretical considerations, the changes in polar lipids we observed during thermal acclimation are likely to promote appropriate membrane fluidity under each thermal regime. The increased incorporation of highly unsaturated fatty acids during cold acclimation could have significant physiological and ecological implications. In particular, since highly unsaturated fatty acids are typically scarce in freshwater food webs, dietary deficiencies in these essential fatty acids may be a significant factor in winter mortality of freshwater alewives.  相似文献   

芥蓝芥子油苷提取条件优化研究     

腊贵晓  孔海民  方萍  杨铁钢 《中国农业科技导报》2014,16(3):150-155

采用正交试验设计,研究了提取温度、提取剂甲醇浓度(V/V)、提取时间和待测物质与提取液比例(简称“物液比”) 对芥蓝菜薹中芥子油苷(总芥子油苷含量、总脂肪族芥子油苷和总吲哚族芥子油苷)提取效果的影响。结果表明,物液比和提取温度对总芥子油苷、总脂肪族芥子油苷和总吲哚族芥子油苷提取效果影响显著(P<0.05),而甲醇浓度和提取时间对芥子油苷提取率无显著影响(P>0.05)。综合考虑提取效果和成本,芥蓝中芥子油苷最佳提取条件为75℃,物液比1∶45(g∶mL),甲醇浓度70%,提取时间5 min。在该条件下,总芥子油苷提取量为8.31 μmol/g DW,总脂肪族芥子油苷提取总量为7.58 μmol/g DW,总吲哚族脂肪族芥子油苷提取总量为0.73 μmol/g DW。  相似文献   

绵羊LDHβ基因的生物信息学分析及其在不同剩余采食量绵羊肝脏和肌肉组织中的表达   总被引：1，自引：1，他引：0  

莫负涛  李发弟  喇永富  张小雪  唐德富  王维民 《甘肃农业大学学报》2017,52(3)

【目的】为鉴定绵羊乳酸脱氢酶β(lactate dehydrogenase B,LDHβ)基因的分子特征,研究其在不同剩余采食量绵羊肝脏和肌肉组织中的表达差异.【方法】测定了137只‘湖羊’公羔的剩余采食量(residual feed intake,RFI),按RFI进行排序,分别筛选出RFI最高(high-residual feed intake,H-RFI)和RFI最低(low-residual feed intake,L-RFI)的羊各15只,屠宰后,采集肝脏和肌肉组织,利用Q-PCR技术检测绵羊LDHβ基因分别在H-RFI和L-RFI羊肝脏和肌肉中表达量,并利用生物信息学软件构建了该基因的系统进化树和预测其结构与功能.【结果】绵羊LDHβ基因开放阅读框(open reading frame,ORF)为1 005bp,编码334个氨基酸,其蛋白质分子质量为36 479.43U,理论等电点为6.40.绵羊LDHβ基因与山羊的亲缘关系较近,其次为牛,序列同源性高.功能结构域预测结果显示,该基因编码产物在内质网中参与辅酶因子生物合成的可能性最高.Q-PCR结果显示,绵羊LDHβ-mRNA基因在L-RFI羊肝脏和肌肉中表达量均显著低于H-RFI羊(P0.01).【结论】绵羊LDHβ基因作为能量代谢的关键酶参与绵羊饲料效率的调控.  相似文献   

Reducing tillage intensity affects the cumulative emergence dynamics of annual grass weeds in winter cereals          下载免费PDF全文

A Scherner  B Melander  P K Jensen  P Kudsk  LA Avila 《Weed Research》2017,57(5):314-322

Annual grass weeds such as Apera spica‐venti and Vulpia myuros are promoted in non‐inversion tillage systems and winter cereal‐based crop rotations. Unsatisfactory weed control in these conditions is often associated with a poor understanding of the emergence pattern of these weed species. The aim of this study was to investigate, understand and model the cumulative emergence patterns of A. spica‐venti, V. myuros and Poa annua in winter cereals grown in three primary tillage regimes: (i) mouldboard ploughing, (ii) pre‐sowing tine cultivation to 8–10 cm soil depth and (iii) direct drilling. Direct drilling delayed the cumulative emergence of A. spica‐venti and V. myuros (counted together) in contrast with ploughing, while the emergence pattern of P. annua was unaffected by the type of tillage system. The total density of emerged weed seedlings varied between the tillage systems and years with a higher total emergence seen under direct drilling, followed by pre‐sowing tine cultivation and ploughing. The emergence patterns of all species were differently influenced by the tillage systems, suggesting that under direct drilling, in which these species occur simultaneously, management interventions should first and foremost consider that A. spica‐venti and V. myuros emerge over a longer period to avoid control failures.  相似文献