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This study is aimed at assessing the ability of metal-resistant yeast, Candida tropicalis, to uptake cadmium from the liquid medium. The minimum inhibitory concentration of Cd2+ against C. tropicalis was 2,800 mg L?1. The yeast also showed tolerance towards Zn2+ (3,100 mg L?1), Ni2+ (3,000 mg L?1), Hg2+ (2,400 mg L?1), Cu2+ (2,300 mg L?1), Cr6+ (2,000 mg L?1), and Pb2+ (1,200 mg L?1). The yeast isolate showed typical growth curves, but low specific rate of growth was observed in the presence of cadmium. The yeast isolate showed optimum growth at 30°C and pH 7. The metal processing ability of the isolate was determined in a medium containing 100 mg L?1 of Cd2+. C. tropicalis could decline Cd2+ 57%, 69%, and 80% from the medium after 48, 96, and 144 h, respectively. C. tropicalis was also able to remove Cd2+ 56% and 73% from the wastewater after 6 and 12 days, respectively. Cd produced an increase in glutathione (GSH) and non-protein thiol levels by 146.15% and 59.67% at 100 mg L?1 concentration, respectively. Metal tolerance and accumulation together with changes in the GSH status and non-protein thiols under Cd exposure were studied in C. tropicalis. 相似文献
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Two pregnant sows were fed on a basal diet consisting of equal amounts of ground barley and ground wheat with or without addition of L-lysine hydrochloride. Plasma amino acid and blood urea levels varied according to the time after feeding. Almost all the essential amino acids reached a maximum level after one hour and then declined at four hours after feeding. The results suggested that samples taken at between one and four hours after feeding were responsive to changes in dietary composition and could provide a measure to evaluate the amino acid pattern in relation to feeding. Both plasma amino acid and blood urea data suggested that lysine was the first limiting amino acid in the basal diet. 相似文献
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Muhammad Naveed Birgit Mitter Sohail Yousaf Milica Pastar Muhammad Afzal Angela Sessitsch 《Biology and Fertility of Soils》2014,50(2):249-262
With the aim to select powerful microbial strains to be used for the enhancement of maize yield and resistance to abiotic and biotic stresses, we tested five endophytic bacterial strains previously isolated from maize roots. A range of different laboratory assays in regard to potential plant growth promotion was performed and strains were further evaluated for improving growth of five maize cultivars under axenic and natural soil conditions. Endophytic colonization was an additional component in our selection process as it is of high importance for an inoculant strain to efficiently colonize the plant environment. All strains had the potential to improve maize seedling growth under axenic conditions. Enterobacter sp. strain FD17 showed both the highest growth-promoting activity under axenic conditions as well as colonization capacity. FD17 was therefore selected for further plant tests in a net house, in which two different maize cultivars were grown in large pots until ripening and subjected to outdoor climatic conditions. Results showed that inoculation significantly increased plant biomass, number of leaves plant?1, leaf area, and grain yield up to 39 %, 14 %, 20 %, and 42 %, respectively, as compared to the un-inoculated control. Similarly, inoculation also improved the photochemical efficiency of photosystem II (PSII) of maize plant and reduced the time needed for flowering. We also confirmed that strain FD17 is able to colonize the rhizosphere, roots and stems. Based on rigorous testing, Enterobacter sp. strain FD17 showed the highest potential to promote growth and health of maize grown under natural conditions. This study suggested that in vitro plant growth-promoting traits and potential of maize seedling growth promotion by bacterial endophytes could be used for the selection of potential inoculant strains subjected for further testing as bio-inoculant under field conditions. 相似文献
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Reid EL Worthy CA Probert I Ali ST Love J Napier J Littlechild JA Somerfield PJ Allen MJ 《Marine drugs》2011,9(4):586-602
Emiliania huxleyi is a single celled, marine phytoplankton with global distribution. As a key species for global biogeochemical cycling, a variety of strains have been amassed in various culture collections. Using a library consisting of 52 strains of E. huxleyi and an 'in house' enzyme screening program, we have assessed the functional biodiversity within this species of fundamental importance to global biogeochemical cycling, whilst at the same time determining their potential for exploitation in biocatalytic applications. Here, we describe the screening of E. huxleyi strains, as well as a coccolithovirus infected strain, for commercially relevant biocatalytic enzymes such as acid/alkali phosphodiesterase, acid/alkali phosphomonoesterase, EC1.1.1-type dehydrogenase, EC1.3.1-type dehydrogenase and carboxylesterase. 相似文献
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Model-Based Evaluation of Maturity Type of Potato Using a Diverse Set of Standard Cultivars and a Segregating Diploid Population 总被引：2，自引：0，他引：2
The objective of this paper is to evaluate the performance of the conventional system of classifying maturity type in potato and to provide a concept of maturity type based on crop physiology. We present an approach in which physiological traits are used to quantify and assess maturity type unambiguously for a set of varieties covering a wide range of maturity classes and a diploid F1 population separating for maturity and well-adapted to Dutch growing conditions, both grown in six environments. We defined physiological maturity based on four traits: the duration of maximum green canopy, the area under the green canopy cover progress curve, and the rate and duration of tuber bulking. The results indicated that physiological maturity type criteria tended to define maturity classes less ambiguously than the conventional criterion. Moreover, the conventional criterion was subject to more random noise and lacked stability and/or repeatability compared with the physiological traits. The physiological maturity criteria also illustrated the physiological trade-offs that existed between the selected traits and underlined the subtle complexities in classifying maturity type. This study highlighted the capabilities of different maturity type criteria in discriminating between different maturity classes among the large set of genotypes. Our new approach involving key physiological traits could be beneficial in offering physiology-based criteria to re-define maturity type. An improved criterion based on important physiological traits would allow relating the maturity to crop phenology and physiology. These new criteria may be amenable to further genetic analysis and could help in designing strategies for potato ideotype breeding for genotypes with specific maturity types. 相似文献
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中小型规模智慧农业物联网终端节点设计 总被引：2，自引：2，他引：0
为了能够向中小规模农业生产经营者提供深度定制的农业物联网技术,该研究研发了一种可用于农业设备信息化的智慧农业物联网终端节点。通过该终端节点将农业设备柔性接入物联网体系,并依托管理服务层App实现应用层App功能开发。系统硬件利用可编程片上系统的IP核重用技术实现对各种农业设备的接入,实现各农业场景中根据设备具体情况进行定制化应用。系统传输层采用LoRa广域网与蓝牙技术,支撑集中式与分布式农业管理服务体系。管理服务层App对底层节点设备操作与Android功能操作进行封装,实现应用层与底层功能结构的解耦,避免了应用层App开发时结构复杂、对底层功能结构变化适应性差、开发周期长等问题。实践应用结果表明,该农业物联网终端节点可有效实现对农业设备的接入,具有1500VDC的电磁隔离能力;节点的LoRa无线数据通道在无严重降雨的天气中数据包传输成功率接近100%,蓝牙可支持周围3m范围内的移动设备现场接入;管理服务层App可有效支撑应用层功能快速开发;在直连上位机模式下,应用层App到节点设备间的功能延时分别小于400与1 700 ms,系统运行稳定,功能支撑可靠。该方法可为国内中小规模农业经营者的物联网信息化建设提供支持与参考。 相似文献
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Determination of liver and muscle aflatoxin B1 residues and select serum chemistry variables during chronic aflatoxicosis in broiler chickens 
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