排序方式： 共有50条查询结果，搜索用时 15 毫秒
31.
J. J. KANEKO D. MATTHEEUWS R. P. ROTTIERS A. VERMEULEN 《The Journal of small animal practice》1978,19(1-12):85-94
The low dose intravenous glucose tolerance test (IVGTT) and the insulin response to the glucose load were performed in a series of twenty–two diabetic dogs. All diabetic dogs were characterized by glucose intolerance as expressed by an abnormal half time (Tl/2) or fractional turnover rate (k) for glucose clearance. On the basis of the initial insulin level (Io), the insulin peak response (Ip) and the insulinogenic index (I/G), the dogs were classified into three types. Type I dogs were characterized by a low Io, low Ip and low I/G in response to glucose, similar to the juvenile form of diabetes in humans. Type II dogs were characterized by a normal or high Io, but also with a low Ip and a low I/G which are some of the features of the maturity onset form. Type III dogs were characterized by a normal Io and a normal or delayed response to glucose as seen in chemical diabetes. It is suggested that these types represent stages in the natural history of the development of diabetes mellitus in dogs. 相似文献
32.
Yasuyuki KANEKO Shidow TORISU Go KITAHARA Yuichi HIDAKA Hiroyuki SATOH Taketoshi ASANUMA Shinya MIZUTANI Takeshi OSAWA Kiyokazu NAGANOBU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):631-635
Laparoscopic cryptorchidectomy without insufflation was applied in 10 standing bulls aged
3 to 15 months. Nine bulls were preoperatively pointed out intra-abdominal testes by
computed tomography. Preoperative fasting for a minimum of 24 hr provided laparoscopic
visualization of intra-abdominal area from the kidney to the inguinal region. Surgical
procedure was interrupted by intra-abdominal fat and testis size. It took 0.6 to 1.5 hr in
4 animals weighing 98 to 139 kg, 0.8 to 2.8 hr in 4 animals weighing 170 to 187 kg, and 3
and 4 hr in 2 animals weighing 244 and 300 kg to complete the cryptorchidectomy. In
conclusion, standing gasless laparoscopic cryptorchidectomy seems to be most suitable for
bulls weighing from 100 to 180 kg. 相似文献
33.
Yasunori SHINOZUKA Kazuhiro KAWAI Tomomi KURUMISAWA Yuko SHIMIZU Tadashi IMANISHI Ayumu OHNO Mano TAKAHASHI Sohei KANEKO Naoki SUZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1620
The aim of this study was to evaluate the microbiota of normal milk in dairy cows and their relationship with host factors, such as the age of the cow (Age), somatic cell counts in milk (SCCs), and days in milk (DIM). We investigated 48 milk samples from 22 cows with no systemic or local clinical signs using MinIONTM nanopore sequencing for a 16S rRNA gene amplicon. Bacterial richness was positively correlated with the DIM (P=0.043), and both the Shannon-Wiener Index and Simpson’s Index, which are metrics of alpha-diversity, were also significantly positively correlated with the SCC (P<0.001). The composition ratios of both Actinobacteria at the phylum level and Kocuria spp. at the genus level in the milk microbiota were significantly correlated with the SCC (P<0.001 and P<0.001, respectively). In the beta-diversity test, the one-way analysis of similarities test showed a significant difference (P=0.0051) between the low- and high-SCC groups. This study clarified that the composition of the normal milk microbiota in this herd was related to the SCC. It also raised the possibility of variations in bacterial genera in the normal milk microbiota between the low- and high-SCC groups. However, to clarify the actual condition of the milk microbiota and to elucidate the relationship with the SCC, it is necessary to perform further analyses taking into account not only the relative abundance, but also the absolute abundance of microbes. 相似文献
34.
Shinnosuke SUZUKI Tomoyuki TSUKIYAMA Takehito KANEKO Hiroshi IMAI Naojiro MINAMI 《The Journal of reproduction and development》2015,61(3):241-244
Transgenic mice are important tools for genetic analysis. A current prominent method for producing transgenic mice involves pronuclear microinjection into 1-cell embryos. However, the total transgenic efficiency obtained using this method is less than 10%. Here, we demonstrate that highly efficient transgenesis in mice can be achieved by cytoplasmic microinjection using a hyperactive piggyBac system. In embryos in which hyPBase mRNA and pPB-CAG-TagRFP DNA were co-injected into the cytoplasm, TagRFP fluorescence was observed after the 2-cell stage; when 30 ng/µl pPB-CAG-TagRFP DNA and 30 ng/µl hyPBase mRNA were co-injected, 94.4% of blastocysts were TagRFP positive. Furthermore, a high concentration of hyPBase mRNA resulted in creation of mosaic embryos in which the TagRFP signals partially disappeared. However, suitable concentrations of injected DNA and hyPBase mRNA produced embryos in which almost all blastomeres were TagRFP positive. Thus, the hyperactive piggyBac transposon system is an easy-to-implement and highly effective method that can contribute to production of transgenic mice. 相似文献
35.
Tamás SOMFAI Nguyen Thi MEN Junko NOGUCHI Hiroyuki KANEKO Naomi KASHIWAZAKI Kazuhiro KIKUCHI 《The Journal of reproduction and development》2015,61(6):571-579
Our aim was to optimize the cryoprotectant treatment for the preservation of immature porcine cumulus-oocyte
complexes (COCs) by solid surface vitrification. In each experiment, the vitrification solution consisted of
50 mg/ml polyvinyl pyrrolidone, 0.3 M of the actual sugar and in total 35% (v/v) of the actual permeating
cryoprotectant (pCPA) combination. After warming, the COCs were subjected to in vitro
maturation, fertilization and embryo culture. In Experiment 1, trehalose and sucrose were equally effective
during vitrification and warming in terms of facilitating oocyte survival and subsequent embryo development.
In Experiment 2, when equilibration was performed at 38.5 C in a total of 4% (v/v) pCPA for 15 min, the
combination of ethylene glycol and propylene glycol (EG + PG = 1:1) was superior to EG and dimethyl sulfoxide
(EG + DMSO = 1:1) in terms of oocyte survival after vitrification and the quality of resultant blastocysts. In
Experiment 3, equilibration in 4% (v/v) pCPA for 15 min before vitrification was superior to that in 15% (v/v)
CPA for 5 min for achievement of high survival rates irrespective of the pCPA combination used. In Experiment
4, when equilibration was performed in 4% EG + PG for 5 min, 15 min or 25 min, there was no difference in
oocyte survival and subsequent embryo development after vitrification and warming; however, the developmental
competence of cleaved embryos was tendentiously reduced when equilibration was performed for 25 min. In
conclusion, trehalose and sucrose were equally effective in facilitating vitrification, and the optimum pCPA
treatment was 5–15 min equilibration in 4% (v/v) of EG + PG followed by vitrification in 35% (v/v) EG +
PG. 相似文献
36.
Yasuyuki KANEKO Shidow TORISU Takumi KOBAYASHI Shinya MIZUTANI Nao TSUZUKI Hiroko SONODA Masahiro IKEDA Kiyokazu NAGANOBU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1633-1638
Arterial blood gas analysis is an important diagnostic and monitoring tool for
respiratory abnormalities. In human medicine, lung complications often occur as a result
of liver disease. Although pulmonary complications of liver disease have not been reported
in dogs, we have frequently encountered hypoxemia in dogs with liver disorders, especially
extrahepatic biliary obstruction. In addition, respiratory disorders account for 20% of
perioperative fatalities in dogs. Therefore, in this study, we evaluated the respiratory
status in dogs with hepatobiliary disease by arterial blood gas analysis. PaO2
and PaCO2 were measured. Alveolar-arterial oxygen difference
(AaDO2), the indicator of gas exchange efficiency, was calculated. Compared to
healthy dogs (control group), hepatobiliary disease dogs had significantly lower
PaO2 and higher AaDO2. Hypoxemia (PaO2 of ≤80 mmHg) was
observed in 28/71 dogs with hepatobiliary disease. AaDO2 was higher (≥30 mmHg)
than the control group range (11.6 to 26.4 mmHg) in 32/71 hepatobiliary disease dogs. By
classifying type of hepatobiliary disease, dogs with extrahepatic biliary obstruction and
chronic hepatitis showed significantly lower PaO2 and higher AaDO2
than in a control group. Dogs with chronic hepatitis also had significantly lower
PaCO2. The present study shows that dogs with hepatobiliary disease have
respiratory abnormalities more than healthy dogs. Preanesthetic or routine arterial blood
gas analysis is likely beneficial to detect the respiratory abnormalities in dogs with
hepatobiliary disease, especially extrahepatic biliary obstruction and chronic
hepatitis. 相似文献
37.
Kazuhiro KIKUCHI Michiko NAKAI Naomi KASHIWAZAKI Hiroyuki KANEKO 《Animal Science Journal》2011,82(4):495-503
In vitro production of embryos, including in vitro maturation, fertilization of oocytes and their subsequent culture to the embryo stage, has become the most popular method of studying gametogenesis and embryogenesis in pigs. As well as their utility for basic studies, these procedures now enable us to generate viable embryos and offspring as a means of conserving genetic resources and rare animal breeds. Recently, more advanced technologies such as xenografting of gonadal (testicular and ovarian) tissues into immunodeficient experimental animals have been developed. In combination with in vitro embryo production techniques, this approach may provide many benefits. We have been carrying out studies to acquire basic information about the application of this method to porcine species, and to improve the existing techniques. Recently, we obtained oocytes from ovarian tissue xenografted and grown in nude mice that had the capacity to be fertilized and the ability to develop into early‐stage embryos. We also obtained spermatozoa from the xenografted testicular tissues and injected them intracytoplasmically into in vitro‐matured oocytes to produce piglets. Here we discuss the further possibilities of conservation and utilization of porcine gonadal tissue by xenografting into immunodeficient mice. 相似文献
38.
Koji MISUMI Yuri HIRAYAMA Misae SUZUKI Michiko NAKAI Hiroyuki KANEKO Junko NOGUCHI Kazuhiro KIKUCHI 《The Journal of reproduction and development》2014,60(3):246-249
The present study was conducted to examine the feasibility of in vitro embryo production and transfer
technologies for producing Middle White piglets. After collection from three retired Middle White sows, a total of 222
oocytes were matured, fertilized and cultured in vitro, and a total of 50 embryos from the 4-cell to
blastocyst stage were produced by the 4th or 5th day. These embryos were transferred individually into three recipients along
with 5 in vivo-derived Duroc blastocysts. All of the recipients became pregnant, and they farrowed a total
of 9 Middle White and 9 Duroc piglets. These results suggest that in vitro embryo production using ovaries
from retired sows is useful for reproduction of pigs of pure breeds including the Middle White for breeding activities and
conservation/utilization of genetic resources. 相似文献
39.
Comparison of Ethylene Glycol and Propylene Glycol for the Vitrification of
Immature Porcine Oocytes
Tamás SOMFAI Michiko NAKAI Fuminori TANIHARA Junko NOGUCHI Hiroyuki KANEKO Naomi KASHIWAZAKI István EGERSZEGI Takashi NAGAI Kazuhiro KIKUCHI 《The Journal of reproduction and development》2013,59(4):378-384
Our aim was to optimize a cryoprotectant treatment for vitrification of immature porcine
cumulus-oocyte complexes (COCs). Immature COCs were vitrified either in 35% ethylene
glycol (EG), 35% propylene glycol (PG) or a combination of 17.5% EG and 17.5% PG. After
warming, the COCs were in vitro matured (IVM), and surviving oocytes were
in vitro fertilized (IVF) and cultured. The mean survival rate of
vitrified oocytes in 35% PG (73.9%) was higher (P<0.05) than that in 35% EG (27.8%).
Oocyte maturation rates did not differ among vitrified and non-vitrified control groups.
Blastocyst formation in the vitrified EG group (10.8%) was higher (P<0.05) than that in
the vitrified PG group (2.0%) but was lower than that in the control group (25.0%).
Treatment of oocytes with 35% of each cryoprotectant without vitrification revealed a
higher toxicity of PG on subsequent blastocyst development compared with EG. The
combination of EG and PG resulted in 42.6% survival after vitrification. The maturation
and fertilization rates of the surviving oocytes were similar in the vitrified, control
and toxicity control (TC; treated with EG+PG combination without cooling) groups.
Blastocyst development in the vitrified group was lower (P<0.05) than that in the
control and TC groups, which in turn had similar development rates (10.7%, 18.1% and
23.3%, respectively). In conclusion, 35% PG enabled a higher oocyte survival rate after
vitrification compared with 35% EG. However, PG was greatly toxic to oocytes. The
combination of 17.5% EG and 17.5% PG yielded reasonable survival rates without toxic
effects on embryo development. 相似文献
40.