Ultrasonographic, computed tomographic and magnetic resonance imaging anatomy of the normal canine stifle joint     

Soler M  Murciano J  Latorre R  Belda E  Rodríguez MJ  Agut A 《Veterinary journal (London, England : 1997)》2007,174(2):351-361

Ultrasonographic (US), magnetic resonance (MR) and computed tomographic (CT) images of normal canine stifle joints were obtained and compared with plastinated cross-sectional slices of cadaver specimens from the same dogs. The bony and articular structures were identified and correlated with the three diagnostic imaging modalities. These results provide an atlas of normal cross-sectional US, MR and CT anatomy of the canine stifle, which can be used for the interpretation of stifle images from any of these imaging modalities.  相似文献   

Seroprevalence and risk factors associated with equineleptospirosis in the metropolitan region of Salvador and Recôncavo Baiano region,Bahia state (NE Brazil)     

Siqueira  Cândida C.  Fraga  Deborah B. M.  Chagas-Junior  Adenizar D.  Athanazio  Daniel A.  Silva  Marta M. N.  Cerqueira  Robson B.  da C. McBride  Flávia W.  Pinna  Melissa H.  Ayres  Maria C. C. 《Tropical animal health and production》2020,52(1):31-39

Tropical Animal Health and Production - Equine leptospirosis, although usually asymptomatic, has been associated with recurrent uveitis, abortion, and other systemic signs, constituting a major...  相似文献   

Urine neutrophil gelatinase-associated lipocalin to diagnose and characterize acute kidney injury in dogs     

Erika Monari  Roberta Troìa  Luca Magna  Marta Gruarin  Chiara Grisetti  Mercedes Fernandez  Andrea Balboni  Massimo Giunti  Francesco Dondi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(1):176-185

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A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets     

Diogo Coelho  Paula A. Lopes  Vânia Cardoso  Patrícia Ponte  Joana Brás  Marta S. Madeira  Cristina M. Alfaia  Narcisa M. Bandarra  Carlos M. G. A. Fontes  José A. M. Prates 《Journal of animal physiology and animal nutrition》2020,104(1):310-321

The main goal of this study was to test a rational combination of pre-selected carbohydrate-active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two-enzyme constituted mixture (Mix), composed by a lysozyme and a α-amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p < .05), as well as a 7-fold increase of oligosaccharides amount (p < .001), were obtained. With resort of fluorescence microscopy, a 36% reduction of fluorescence intensity (p < .001) was observed using Calcofluor White staining. In the supernatant, the Mix caused a 1.34-fold increase in protein content (p = .018) relative to the control. Similarly, n-6 polyunsaturated fatty acids (PUFA) (p = .007), in particular 18:2n-6 (p = .016), monounsaturated fatty acids (MUFA) (p = .049) and chlorophyll a (p = .025) contents were higher in the supernatant of microalgae treated with the enzyme mixture in relation to the control. Taken together, these results point towards the disclosure of a novel two-enzyme mixture able to partial degrade A. platensis cell wall, improving its nutrients bioavailability for monogastric diets with the cost-effective advantage use of microalgae in animal feed industry.  相似文献   

Single injection of triptorelin or buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG     

Marta Dordas-Perpinyà  Laure Normandin  Thibault Dhier  Hubert Terris  Anaïs Cochard  Camille Frilley  Fanny Huiban  Jean-François Bruyas 《Reproduction in domestic animals》2020,55(3):374-383

The aim of this study was to assess the efficacy of different doses of buserelin acetate and another GnRH agonist, triptorelin acetate, in saline solution in a single subcutaneous injection, to induce ovulation of growing pre-ovulatory follicle in mare and compare it with the classical treatment of a single injection of hCG. The study is split into 3 experiments over different breeding seasons in the same stud with a random distribution of treatment. The first one was to compare the injection of 6 mg of buserelin with 1,500 IU of hCG; the second one consisted of comparing different doses of buserelin (6 mg and 3 mg); and the third one compared three different doses of buserelin (3, 2 and 1 mg), 0.1 mg of triptorelin with 1,500 IU of hCG as a control group. The results of all experiments showed the same efficacy between all treatments with mares ovulating between 24 and 48 hr after injection: experiment 1: hCG (78% n = 41) and buserelin 6 mg (90% n = 50); experiment 2: buserelin 6 mg (78,1% n = 192) and buserelin 3 mg (78% n = 341); and experiment 3: hCG (87% n = 106), buserelin 3 mg (84,7% n = 137), buserelin 2 mg (82,7% n = 104), buserelin 1 mg (87% n = 54) and triptorelin 0.1 mg (84,7% n = 72). In conclusion, this study contributes to erasing the dogma that has been established since 1975 that a single injection in solution without any long-acting excipient of a GnRH agonist cannot induce ovulation in the mare. This study also shows that a injection of 0.1 mg of triptorelin in solution is a good alternative for ovulation induction and is comparable to small doses of buserelin acetate in solution (1 mg) and 1,500 IU of the gold standard trigger hCG, mainly in countries where human formulation of buserelin is not available.  相似文献   

Canine semen cryoconservation: Emerging data over the last 20 years     

Djemil Bencharif  Marta Dordas-Perpinya 《Reproduction in domestic animals》2020,55(Z2):61-65

Canine semen cryoconservation was used since 1969, and this process is still nowadays in progress. This review aims to have an overview of two factors leading to a successful freezing–thawing semen. The success and efficiency of freezing process can be measured by the post-thawing sperm mobility. The first factor is the best extender used as a cryoprotectant to have a similar osmolarity and pH compared to the seminal plasma to enable sperm survival. Historically, chicken egg yolk was used since 1940, but due to microbial risks and to the presence of granules (which interfere with counting dead spermatozoa and inhibits a spermatozoal respiration), despite these disadvantages, egg yolk is considered an excellent cryoprotectant for sperm of different animal species. The low-density lipoproteins (LDL), contained in EY, when used at a concentration of 6% in a freezing medium associated with 20 mM of glutamine, show a mobility up to 54.5%, which is the best combination found. However, the sperm protection mechanism by LDL during freezing–thawing process only begins to be decrypted. But extraction protocols of LDL are not efficient for an industrial use. Therefore, egg yolk plasma is used within liquid or lyophilized state, and offering similar efficiency as the 6% LDL middle. The equilibration step, in which the diluted sperm is placed for a variable period of time at a temperature of +4°C, before freezing it. The studies show that 6 hr is the optimal duration for the canine sperm equilibration. The future of canine sperm cryopreservation is expected in liposome use and synthetic substances, which mimics LDL role.  相似文献   

Decorin concentrations in canine normal and neoplastic mammary gland tissues     

Marek Szczubiał  Marta Kankofer  Jacek Wawrzykowski  Roman Dąbrowski  Wojciech Łopuszyński  Mariola Bochniarz  Piotr Brodzki 《Reproduction in domestic animals》2020,55(10):1404-1410

In the present study, the concentration of decorin in canine normal and neoplastic mammary gland tissues was examined to understand the potential role of decorin in development and progression of canine mammary tumours. The homogenates of 48 mammary gland tumours (10 benign and 38 malignant) and 10 samples of normal canine mammary gland tissue were used in the study. The presence and quantification of decorin was examined in the homogenates using Western blot and specific canine ELISA. Western blotting confirmed the presence of decorin both in the normal mammary gland tissues and in the mammary gland tumours. The concentration of decorin was significantly higher (p < .05) in the benign tumours and non-metastatic malignant tumours than in the normal mammary gland. The concentration of decorin was significantly lower (p < .05) in the malignant tumours with metastasis to regional lymph nodes compared with benign tumours and non-metastatic malignant tumours. No significant differences were found in the level of decorin between the benign and the non-metastatic malignant tumours. Both the histological type of malignant tumours and the histological grade did not significantly affect the concentration of decorin. These findings suggest that neoplastic transformation in the canine mammary gland leads to increase in the decorin protein synthesis. The reducing decorin concentration in canine malignant mammary tumours appears to facilitate the metastatic spread of these tumours.  相似文献   

Thoracic paravertebral block for an opioid-free thoracotomy in a dog     

Marta Garbin  Gianluca Bertolizio  Diego A. Portela 《Veterinary anaesthesia and analgesia》2021,48(4):622-623

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Evaluation of the effect of ultrasound guidance on the accuracy of intercostal nerve injection: a canine cadaveric study     

Alexander C.S. Thomson  Diego A. Portela  Marta Romano  Pablo E. Otero 《Veterinary anaesthesia and analgesia》2021,48(2):256-263

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A modelling approach to evaluate land management options for recently burnt catchments     

Marta Basso  Dalila Serpa  João Rocha  Martinho A. S. Martins  Jacob Keizer  Diana C. S. Vieira 《European Journal of Soil Science》2022,73(4):e13275

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