Morphological comparison of wild,farmed and hybrid specimens of two South American silversides,Odontesthes bonariensis and Odontesthes hatcheri          下载免费PDF全文

Sonia Alejandra Crichigno  Ricardo Shohei Hattori  Carlos Augusto Strüssmann  Víctor Cussac 《Aquaculture Research》2016,47(6):1797-1808

In this study, body shape of hybrid and presumptive introgressed South American silversides was studied. Body shape of O. bonariensis and O. hatcheri from wild populations and farmed stocks was compared to provide basic information on the effects of fish farming on morphometric parameters. Subsequently, wild presumptive introgressed individuals and artificially hybridized farmed individuals were morphologically analysed to assess the effects of hybridization on the same parameters. Most farmed purebred individuals were shorter and higher than their wild counterparts, which is probably due to the favourable growth conditions compared to the wild habitat. However, the results evidenced that purebred individuals were more slender than both hybrid (farmed) fish and introgressed (wild) fish. Further studies on the growth performance of hybrid Odontesthes will be required in order to assess whether the combination of hybridization and sterilization could produce, under farming conditions, growth performances which satisfy the requirements of aquaculture.  相似文献   

Feedbacks between vegetation pattern and resource loss dramatically decrease ecosystem resilience and restoration potential in a simple dryland model     

Ángeles G. Mayor  Sonia Kéfi  Susana Bautista  Francisco Rodríguez  Fabrizio Cartení  Max Rietkerk 《Landscape Ecology》2013,28(5):931-942

Conceptual frameworks of dryland degradation commonly include ecohydrological feedbacks between landscape spatial organization and resource loss, so that decreasing cover and size of vegetation patches result in higher water and soil losses, which lead to further vegetation loss. However, the impacts of these feedbacks on dryland dynamics in response to external stress have barely been tested. Using a spatially-explicit model, we represented feedbacks between vegetation pattern and landscape resource loss by establishing a negative dependence of plant establishment on the connectivity of runoff-source areas (e.g., bare soils). We assessed the impact of various feedback strengths on the response of dryland ecosystems to changing external conditions. In general, for a given external pressure, these connectivity-mediated feedbacks decrease vegetation cover at equilibrium, which indicates a decrease in ecosystem resistance. Along a gradient of gradual increase of environmental pressure (e.g., aridity), the connectivity-mediated feedbacks decrease the amount of pressure required to cause a critical shift to a degraded state (ecosystem resilience). If environmental conditions improve, these feedbacks increase the pressure release needed to achieve the ecosystem recovery (restoration potential). The impact of these feedbacks on dryland response to external stress is markedly non-linear, which relies on the non-linear negative relationship between bare-soil connectivity and vegetation cover. Modelling studies on dryland vegetation dynamics not accounting for the connectivity-mediated feedbacks studied here may overestimate the resistance, resilience and restoration potential of drylands in response to environmental and human pressures. Our results also suggest that changes in vegetation pattern and associated hydrological connectivity may be more informative early-warning indicators of dryland degradation than changes in vegetation cover.  相似文献   

Dissipation of fungicides in a vineyard soil amended with different spent mushroom substrates     

Marín-Benito JM  Andrades MS  Sánchez-Martín MJ  Rodríguez-Cruz MS 《Journal of agricultural and food chemistry》2012,60(28):6936-6945

The degradation kinetics and formation of metabolites for fungicides of different chemical classes (iprovalicarb, metalaxyl, penconazole, and pyrimethanil) and determination of bound residues for metalaxyl and penconazole were studied in both an unamended vineyard soil and in the same soil amended with two spent mushroom substrates (composted (C-SMS1) and fresh (F-SMS2)). The degradation kinetics was fitted to single first-order or first-order multicompartment patterns. Degradation rates decreased in C-SMS1-amended soils for all fungicides as compared to unamended soil, but in F-SMS2-amended soils, they decreased only for iprovalicarb and penconazole. The DT(50) values were higher by up to 1.8 (metalaxyl), 3.8 (pyrimethanil), 4.1 (iprovalicarb), and >1000 (penconazole) times in the soil plus C-SMS1 compared to those for soil plus F-SMS2 or unamended soil. The dissipation mechanism recorded the highest mineralization in the unamended soil for (14)C-metalaxyl and (14)C-penconazole, with the highest formation of nonextractable residues in the F-SMS2-amended soil for (14)C-metalaxyl. The results are consistent with (1) the chemical characteristics of each SMS (total and soluble organic carbon) controlling sorption and the bioavailability of fungicides and (2) the microbial activity of SMS-amended soils, which affects fungicide biodegradation. The findings of this work highlight the potential of SMS amendments with different characteristics to decrease or increase the degradation rate of a fungicide in a vineyard soil.  相似文献   

Food-derived peptides stimulate mucin secretion and gene expression in intestinal cells     

D Martínez-Maqueda  B Miralles  S De Pascual-Teresa  I Reverón  R Muñoz  I Recio 《Journal of agricultural and food chemistry》2012,60(35):8600-8605

In this study, the hypothesis that food-derived opioid peptides besides β-casomorphin 7 might modulate the production of mucin via a direct action on epithelial goblet cells was investigated in HT29-MTX cells used as a model of human colonic epithelium. Seven milk whey or casein peptides, a human milk peptide, and a wheat gluten-derived peptide with proved or probable ability to bind μ- or δ-opioid receptors were tested on the cell culture. Significantly increased secretion of mucins was found after exposure to six of the assayed peptides, besides the previously described β-casomorphin 7, as measured by an enzyme-linked lectin assay (ELLA). Human β-casomorphin 5 and α-lactorphin were selected to study the expression of mucin 5AC gene (MUC5AC), the HT29-MTX major secreted mucin gene. α-Lactorphin showed increased expression of MUC5AC from 4 to 24 h (up to 1.6-fold over basal level expression), although differences were statistically different only after 24 h of exposure. However, this increased expression of MUC5AC did not reach significance after cell treatment with human β-casomorphin 5. In conclusion, six food-derived peptides have been identifed with described or probable opioid activity that induce mucin secretion in HT29-MTX cells. Concretely, α-lactorphin is able to up-regulate the expression of the major secreted mucin gene encoded by these cells.  相似文献   

Grape varieties (<Emphasis Type="Italic">Vitis vinifera</Emphasis> L.) from the Balearic Islands: genetic characterization and relationship with Iberian Peninsula and Mediterranean Basin     

Sonia García-Muñoz  Thierry Lacombe  M. Teresa de Andrés  Laura Gaforio  Gregorio Muñoz-Organero  Valérie Laucou  Patrice This  Félix Cabello 《Genetic Resources and Crop Evolution》2012,59(4):589-605

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Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification     

Ferret-Bernard S  Lacroix-Lamandé S  Remot A  Metton C  Bernardet N  Charley B  Drouet F  Laurent F 《Veterinary research》2011,42(1):19

ABSTRACT: At birth, the immune system is still in development making neonates more susceptible to infections. The recognition of microbial ligands is a key step in the initiation of immune responses. It can be mimicked to stimulate the immune system by the use of synthetic ligands recognising pattern recognition receptors. In human and mouse, it has been found that neonatal cytokine responses to toll-like receptor (TLR) ligands differ in many ways from those of adults but the relevant studies have been limited to cord blood and spleen cells. In this study, we compared the responses in neonate and adult sheep to CpG oligodeoxynucleotides (ODN), a TLR9 ligand, in both a mucosal and a systemic organ. We observed that in response to CpG-ODN more IL-12 was produced by neonatal than adult sheep cells from mesenteric lymph nodes (MLN) and spleen. This higher IL-12 response was limited to the first 20 days after birth for MLN cells but persisted for a longer period for spleen cells. The major IL-12-producing cells were identified as CD14+CD11b+. These cells were poor producers of IL-12 in response to direct stimulation with CpG-ODN and required the cooperation of other MLN cells. The difference in response to CpG-ODN between neonates and adults can be attributed to both a higher proportion of CD14+CD11b+ cells in neonate lambs and their higher capacity to produce IL-15. The IL-15 increases IL-12 production by an amplifying feedback loop involving CD40.  相似文献   

Effect of the US3 protein of bovine herpesvirus 5 on the actin cytoskeleton and apoptosis     

Ladelfa MF  Kotsias F  Del Médico Zajac MP  Van den Broeke C  Favoreel H  Romera SA  Calamante G 《Veterinary microbiology》2011,153(3-4):361-366

The US3 protein is a unique protein kinase only present in the Alphaherpesvirinae subfamily of the herpesviruses. Studies performed with several alphaherpesviruses demonstrated that the US3 protein is involved in cytoskeleton modifications during viral infection and displays anti-apoptotic activity. However, the US3 protein of BoHV-5 has not been studied up to now. As reported for other alphaherpesviruses, our results showed that BoHV-5 US3 confers resistance against apoptosis and induces cytoskeletal reorganization leading to cell rounding, actin stress fiber breakdown and cell projections that interconnect cells. The expression of a kinase-dead version of BoHV-5 US3 showed that the anti-apoptotic activity and the induction of cell projections are kinase-dependent whereas kinase activity is not absolutely required for actin stress fiber breakdown. Besides, the kinase-dead version of US3, but not the wild type protein, was found excluded from the nucleus. These results constitute the first report on the BoHV-5 US3 functions, and highlight that there are functional differences and similarities among US3 proteins of different alphaherpesviruses.  相似文献   

Assessment of PCDD/Fs and PCBs in Sediments from the Spanish Northern Atlantic Coast     

Gómez-Lavín  Sonia  Gorri  Daniel  Irabien  Ángel 《Water, air, and soil pollution》2011,214(1-4):287-295

Fluid catalytic cracking of heavy ends to high-value liquid fuels is a common unit operation in oil refineries. In this process, the heavy feedstock that contains sulfur is cracked to light products. Sulphur content is hence redistributed in the liquid and gaseous products and coke of the catalyst used in this process. The coke is later burnt in the regenerator releasing sulfur into the discharged flue gas as SO₂. In the present work, comprehensive emission inventories for a fluid catalytic cracking unit in a typical oil refinery are prepared. These inventories are based on calculations that assume complete combustion of catalyst coke in the regenerator. Yearly, material balances for both SO₂ and particulate matters emissions are carried out taking into account seasonal variations in the operation of the process unit. The results presented in this article reflect the variation of sulfur in feedstock originating from various units in the refinery. The refinery operations are not dependant on seasons but controlled by market-driven conditions to maximize the profit. The seasonal impact on refinery emissions is minimal due to its operation at optimum capacity fulfilling the international market demand. The data presented and analyzed here can be used to assess the hazardous impact of SO₂ and particulate matter emissions on surrounding areas of the refinery.  相似文献   

Molecular detection of important abortion-causing microorganisms in preputial swab of breeding bucks using PCR-based assays     

Chetna Gangwar  Gururaj Kumaresan  Anil Kumar Mishra  Anshuman Kumar  Anjali Pachoori  Sonia Saraswat  Narendra Pratap Singh  Suresh Dinkar Kharche 《Reproduction in domestic animals》2020,55(11):1520-1525

Infectious diseases and aetiological agents related to female reproductive systems were extensively covered compared to its male counterpart. There needs a proper study to bridge this gap, where microflora and infectious agents of both male and female reproductive are mutually intelligible. With this study, we aimed to evaluate the microbial contamination of the preputial cavity and also screened for abortion-causing agents which are zoonotic as well. In goats, such types of abortions are caused by Brucella melitensis, Chlamydophila, Campylobacter and Coxiella etc. One of the major sources of contamination of semen is the preputial cavity, which is exposed to the external environment leading to spread of infection into the female via semen straws or by natural service. In the current study, good quality bucks (n = 32, Barbari = 12, Jamunapari = 10, Jakhrana = 10) which were routinely used for semen collection were screened for their preputial swabs, for the presence of the above pathogens. For detection of Brucella melitensis, OMP31 based TaqMan® probe real-time PCR assay was used, and for Chlamydia, 16srRNA gene based SYBR® green real-time PCR assay was employed for detection of Chlamydophila abortus. While for Campylobacter spp. and Coxiella burnetii, 16srRNA gene based conventional PCR and Trans-PCR were used, respectively. In the current study, of the screened preputial swabs, none of them showed positive for Brucella and Coxiella, but of the screened 32 samples 17 showed positive for Chlamydia (53.13%) and two (6.25%) showed positive for Campylobacter spp. The current study emphasizes on the farms and laboratories which were regularly involved in screening of brucellosis also often overlook the other potential non-brucella pathogens, causing abortions eventually incurring severe economic losses to the goat keepers.  相似文献   

Effects of supplemental‐nitrogen on the quality of rice proteins     

Sonia R. Souza  Elvia Mariam L. M. Stark  Manlio S. Fernandes 《Journal of plant nutrition》2013,36(9):1739-1751

A study was conducted on the effect of supplemental nitrogen (N) (20 hg/ha) applied as a foliar spray or to the soil on seed production, protein percentage, and protein fractions of rice. Plants were grown in a greenhouse over two different periods of time, i.e., August 1988 to January 1989 (Period I), and December 1988 to April 1989 (Period II). Nitrogen was applied to the leaves 10 and 20 days after anthesis (DAA), and to the soil at anthesis and at 15 DAA. Average temperature was 28.7°C during Period I and 32°C during Period II, corresponding to 18.7 and 22.0 growing degree‐day/day (GDD/day), respectively. The difference in GDD/day reduced the plant cycle from 130 days during Period I to 109 days during Period II. Plants grown during Period II had larger numbers of spikelets, a higher percentage of “full grown grains”;, and higher grain weight. Although percentage crude protein was about the same for the two periods, prolamin content was increased and the albumin+globulin fraction was decreased during Period II, but with no difference in glutelin content. The increase in number of spikelets, percent full grains, and grain weight appeared to result in a greater energy demand for plants grown during Period II. This may explain the increase in prolamins, since prolamin synthesis requires less energy than globulin or albumin synthesis. There was a simultaneous decrease in albumin and globulin synthesis during Period II. The content of glutelins, which represent the major reserve proteins in rice grains, was constant during both periods.  相似文献