Recurrence rates and sites for grade II canine cutaneous mast cell tumors following complete surgical excision.     

Chick Weisse  Frances S Shofer  Karin Sorenmo 《Journal of the American Animal Hospital Association》2002,38(1):71-73

A retrospective study was performed on 31 dogs with completely excised, grade II, cutaneous mast cell tumors in order to determine recurrence rates and sites. Distant tumor recurrence developed in 22% of dogs, and local tumor recurrence developed in 11% of dogs; however, the vast majority of these animals were incompletely staged initially. Complete surgical excision of grade II mast cell tumors was associated with effective local control in 89% of these dogs. Therefore, adjuvant radiation therapy might not be indicated in the majority of dogs with complete surgical excision.  相似文献   

用SSR标记分析豫综5号和金皇后综合种的遗传变异   总被引：1，自引：0，他引：1  

吴连成  侯本军  库丽霞  王铁固  孟庆雷  陈彦惠 《华北农学报》2007,22(3):30-34

利用40对SSR标记引物分析了豫综5号和金皇后综合种的遗传变异.结果表明:40对引物在两群体中都扩增出115个多态位点;累加40对引物扩增的基因型种类,豫综5号196种,金皇后194种;豫综5号的平均遗传距离为0.383 4,金皇后综合种为0.339 7;豫综5号的平均观察杂合度是0.382 6,平均期望杂合度是0.474 7;金皇后综合种的平均观察杂合度是0.329 2,平均期望杂合度是0.414 3.从以上结果来看,豫综5号的遗传变异较丰富.  相似文献   

利用若干单株混合提取DNA方法进行玉米群体SSR的分析   总被引：9，自引：1，他引：9  

王铁固  陈彦惠  吴连成  库丽霞  侯本军 《华北农学报》2005,20(1):6-11

以金皇后玉米群体90个单株：DNA和6个自交系DNA为供试材料，利用15对SSR引物，比较了同一群体4种DNA样品处理(单株DNA样品、3个单株混合DNA样品、10个单株混合DNA样品、15个单株混合DNA样品)的SSR遗传多态性。结果表明，利用单株DNA样品能获得等位基因数、基因频率、基因型杂合度等较全面的遗传信息，适宜进行单个或少量群体的遗传结构研究，但试验工作量大；利用混合DNA样品所能获得的遗传信息有所减少，比较4种DNA样品处理的检测结果发现，15个单株混合样品的检测结果误差较大，而采用3～10个单株DNA混合的样品基本可以反映出一个群体的等位基因数目和SSR带型的差异，由于试验工作量大大减少，可以应用于较多玉米群体间遗传差异的比较。  相似文献   

Preliminary study on the molecular mechanism of photoinhibition of rice photosynthesis Chinese Rice Research Newsletter     

ZHANG Deyu  JIAO Demao  Wu Ningyi  DI Lie  Wu Rongsheng  Maurice S.B. KU  XU Xianxiu 《水稻科学》1994,2(3):5

  相似文献   

Canine mammary gland tumours; a histological continuum from benign to malignant; clinical and histopathological evidence*     

K. U. Sorenmo  V. M. Kristiansen  M. A. Cofone  F. S. Shofer  A.‐M. Breen  M. Langeland  C. M. Mongil  A. M. Grondahl  J. Teige  M. H. Goldschmidt 《Veterinary and comparative oncology》2009,7(3):162-172

This study describes the clinical and histopathological findings in dogs with mammary gland tumours, and compares the histopathological and clinical evidence consistent with progression from benign to malignant to human breast cancer epidemiology. Clinical and histopathological data on 90 female dogs with 236 tumours was included. Dogs with malignant tumours were significantly older than dogs with benign tumours (9.5 versus 8.5 years), P = 0.009. Malignant tumours were significantly larger than benign tumours (4.7 versus 2.1 cm), P = 0.0002. Sixty‐six percent had more than one tumour, and evidence of histological progression was noted with increasing tumour size. Dogs with malignant tumours were significantly more likely to develop new primary tumours than dogs with benign tumours, P = 0.015. These findings suggest that canine mammary tumours progress from benign to malignant; malignant tumours may be the end stage of a histological continuum with clinical and histopathological similarities to human breast carcinogenesis.  相似文献   

豫综5号玉米综合种淀粉含量轮回选择效果分析     

库丽霞  王付娟  郭书磊  谢丽莉  刘海静  张君  田磊  陈彦惠 《中国农业科学》2012,45(8):1636-1643

【目的】评价豫综5号玉米群体淀粉含量的改良效果,为创制高淀粉玉米种质资源、选育高淀粉玉米杂交种和继续改良豫综5号的淀粉含量提供依据。【方法】以提高玉米群体淀粉含量为目标,利用近红外技术对豫综5号群体完成了4轮的群体改良。以改良群体为父本,以黄早4、丹340、齐319、掖478和Mo17为测验种,采用NC-II遗传交配设计组配成20个测交组合。通过对不同轮次改良群体和20个测交组合在2个环境中的评价试验,对豫综5号群体淀粉含量轮回选择的改良效果进行了比较研究。【结果】豫综5号群体的淀粉含量随着改良轮次的增加呈逐渐提高的趋势,由C0的69.39%增加到C4的72.31%,4轮选择后淀粉含量实际增益为2.92%。同时蛋白质和赖氨酸含量呈逐轮下降趋势。随着改良轮次的增加,各测验种与各轮改良群体测交组合的淀粉含量逐轮提高,说明非破坏性单粒淀粉含量的轮回选择对豫综5号淀粉含量的改良是有效的;就一般配合力而言,随着改良轮次的增加,淀粉含量的一般配合力呈逐轮提高的趋势;从特殊配合力分析结果表明,黄早4与各轮群体间特殊配合力效应值逐轮提高,其它4个测验种与群体特殊配合力效应值呈现一定的波动。【结论】利用非破坏性单粒淀粉含量的轮回选择改良豫综5号淀粉含量是有效的,随着改良轮次的增加,豫综5号的淀粉含量逐渐提高。  相似文献   

八种中式烹饪工艺对牛肉中多环芳烃、反式脂肪酸和亚硝酸盐的影响   总被引：3，自引：1，他引：2  

张兰  高天丽  刘永峰  赵晶  廖晶  库婷 《中国农业科学》2017,50(6):1126-1138

【目的】从蒸、煮、炖、烤、炸、煎、干制及腌制八种传统中式烹饪工艺中筛选获得有害物质较少的牛肉处理工艺,为消费者选择有害物质含量较低的烹饪工艺提供理论参考。【方法】通过调控烤、炸、煎3种高温处理的温度和时间,对比分析多环芳烃(PAHs)、反式油酸(C_(18:1 trans-9))及亚硝酸盐3类有害物质的组分及含量,选出较优的烤、炸、煎烹饪条件;在此基础上,综合分析烤、炸、煎与蒸、煮、炖、干制及腌制8种烹饪工艺对牛肉中有害物质的影响,进而选择较优的中式烹饪工艺。试验分别采用高效液相色谱外标法、气相色谱-质谱外标法及分光光度法测定牛肉中PAHs、C_(18:1 trans-9)及亚硝酸盐的含量。【结果】对照组中共检测出芘、苯并[a]蒽、苣和苯并[k]荧蒽4种,烤制以160—180℃处理为较优,有5种PAHs且含量低,炸制以3—4 min处理含量较低,煎制以2—3 min处理含量较低;8种中式烹饪中,炸、煎的肉样中PAHs种类最多,炸、腌制的肉样中苯并[a]蒽含量较高,蒸制及煎制的肉样中苣含量较高,烤、炸、煎的肉样中苯并[b]荧蒽含量较高,炸、煎的肉样中苯并[k]荧蒽、苯并[a]芘和二苯并[a,h]蒽含量较高。烤制温度对C_(18:1 trans-9)含量影响不显著(P0.05),炸制3 min和煎制2min的肉样中C_(18:1 trans-9)含量均最低(P0.05);8种中式烹饪中,炸、煎的肉样中C_(18:1 trans-9)含量较高(P0.05)。烤制160℃和180℃的肉样中亚硝酸盐含量均较低,炸制3 min和煎制2 min的亚硝酸盐含量最低(P0.05);8种烹饪工艺中,炸、煎及腌制的肉样中亚硝酸盐含量较高(P0.05)。【结论】对烤、炸、煎3种高温处理,肉样在160℃下烤制40 min,在226—228℃下炸制3 min、煎制2 min时3类有害物质含量较低;综合分析8种工艺,蒸制、煮制、炖制及干制的肉样中3类有害物质种类较少且含量较低。  相似文献   

Efficacy of doxorubicin‐based chemotherapy for non‐resectable canine subcutaneous haemangiosarcoma     

J. L. Wiley  K. A. Rook  C. A. Clifford  T. P. Gregor  K. U. Sorenmo 《Veterinary and comparative oncology》2010,8(3):221-233

Eighteen dogs with measurable subcutaneous haemangiosarcoma (SQHSA) were treated with doxorubicin‐based chemotherapy. Response assessment was evaluated and compared using World Health Organization (WHO), Response Evaluation Criteria in Solid Tumours (RECIST) and tumour volume criteria. The overall response rate for all dogs was 38.8% using WHO criteria, 38.8% using RECIST criteria and 44% using tumour volume criteria. One dog had a complete response. The median response duration for all dogs was 53 days (range 13–190 days). Four dogs had complete surgical excision after neoadjuvant chemotherapy. The median progression‐free interval for dogs with complete surgical excision after neoadjuvant chemotherapy was significantly longer than those not having surgical excision (207 days versus 83 days, respectively) (P = 0.003). No significant difference in metastasis‐free interval or survival time was found between the groups. Doxorubicin‐based chemotherapy appears to be effective for non‐resectable canine SQHSA, although the response duration is relatively short.  相似文献   

Outcome and toxicity associated with a dose‐intensified,maintenance‐free CHOP‐based chemotherapy protocol in canine lymphoma: 130 cases     

K. Sorenmo  B. Overley  E. Krick  T. Ferrara  A. LaBlanc  F. Shofer 《Veterinary and comparative oncology》2010,8(3):196-208

A dose‐intensified/dose‐dense chemotherapy protocol for canine lymphoma was designed and implemented at the Veterinary Hospital of the University of Pennsylvania. In this study, we describe the clinical characteristics, prognostic factors, efficacy and toxicity in 130 dogs treated with this protocol. The majority of the dogs had advanced stage disease (63.1% stage V) and sub‐stage b (58.5%). The median time to progression (TTP) and lymphoma‐specific survival were 219 and 323 days, respectively. These results are similar to previous less dose‐intense protocols. Sub‐stage was a significant negative prognostic factor for survival. The incidence of toxicity was high; 53.9 and 45% of the dogs needed dose reductions and treatment delays, respectively. Dogs that required dose reductions and treatment delays had significantly longer TTP and lymphoma‐specific survival times. These results suggest that dose density is important, but likely relative, and needs to be adjusted according to the individual patient's toxicity for optimal outcome.  相似文献   

Determination of the Photoperiod-Sensitive Inductive Phase in Maize with Leaf Numbers and Morphologies of Stem Apical Meristem     

WU Lian-cheng  WANG Tie-gu  KU Li-xia  HUANG Qun-ce  SUN Zhao-hui  XIA Zhong-liang  CHEN Yan-hui 《中国农业科学(英文版)》2008,7(5):554-560

It is vital to determine the effective photoperiods of maize for making full use of tropical germplasm, which is the foundation for determining the effect of latitude and planting date on the development of photoperiod-sensitive maize cultivars. The objective of this study is to determine the photoperiod-sensitive inductive phase using reciprocal transfer between long- day （LD） （15 h d^-1） and short-day conditions （SD） （9 h d^-1）. For Huangzao 4 and CML288, days to tassel and pollen shedding were recorded, and stem apical meristems （SAM） were observed by a laser scanning confocal microscope. The results show that the seedlings are insensitive to photoperiod when they are very young （juvenile）. However, after this period, LD delays flowering and increases the leaf numbers below the inflorescence, and the length of the interval of the photoperiod-sensitive inductive phase is longer under LD conditions than under SD conditions. Transferred from SD to LD, plants show a sudden decrease in leaf numbers once sufficient SD has been received for flower commitment. While transferred from LD to SD, plants have a continuous increase in leaf numbers during the photoperiod sensitive inductive phase under LD conditions. At the same time, when plants are competent to flowers, the obvious morphology is the elongation of maize SAM. There is an obvious variance of the photoperiod sensitive phase under LD and SD conditions in different maize.  相似文献