Evaluation of Adjuvant Doxorubicin-Based Chemotherapy for the Treatment of Feline Mammary Carcinoma     

C.J. McNeill  K.U. Sorenmo  F.S. Shofer  L. Gibeon  A.C. Durham  L.G. Barber  J.L. Baez  B. Overley 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(1):123-129

Background: Feline mammary carcinomas (FMC) are locally invasive and highly metastatic tumors. Because of the high metastatic potential, patients often are treated with adjuvant doxorubicin-based chemotherapy, but little data exist to evaluate the effect of this strategy.
Hypothesis: Adjuvant doxorubicin-based chemotherapy improves outcome for FMC compared with surgery alone.
Animals: Cats with naturally occurring, biopsy-confirmed FMC treated with either surgery alone (Sx) or with surgery plus adjuvant doxorubicin-based chemotherapy (Sx + Chemo).
Methods: Retrospective cohort study. Clinical data were collected and compared to identify differences between groups. Outcome results were determined and compared. Prognostic factors for disease-free survival (DFS) and overall survival were evaluated.
Results: Seventy-three cats were evaluated, of which 37 were in the Sx group and 36 in the Sx + Chemo group. No differences in clinical data were found between Sx and Sx + Chemo groups. Median DFS times for the Sx and Sx + Chemo groups were 372 and 676 days, respectively ( P = .15) and median survival times (ST) were 1,406 and 848 days, respectively ( P = .78). For cats that underwent a unilateral radical mastectomy, ST was significantly longer for the Sx + Chemo compared with the Sx group (1,998 versus 414 days, respectively; P = .03).
Conclusions and Clinical Importance: This study did not find a benefit to adjuvant doxorubicin-based chemotherapy in cats with FMC. Additional studies are required to determine whether patient subgroups with negative prognostic factors may benefit from adjuvant chemotherapy.  相似文献   

Expression of ABC-Transport Proteins in Canine Mammary Cancer: Consequences for Chemotherapy     

KU Honscha  A Schirmer  A Reischauer  H-A Schoon  A Einspanier  G Gäbel 《Reproduction in domestic animals》2009,44(S2):218-223

Intrinsic or acquired drug resistance is a major barrier for chemotherapy of cancer. Importantly, the presence of ATP-binding cassette, ABC-transport proteins in tumour cells circumvents an intracellular accumulation of chemotherapeutic drugs. In this study, 103 canine mammary tumour probes were investigated for mRNA expression of seven ABC-transporters by RT-PCR. All tumour samples expressed multidrug resistance-associated protein 1 (MRP1) and breast cancer resistance protein (BCRP). MRP7 was detected in 97.1% of tumour probes, MRP3 in 96.1%, Pgp in 92.2%, MRP5 in 85.4% and MRP6 in 64.1%. More of the half of tumour samples (56.1%) expressed all of the examined ABC-transport proteins. Approximately one-third of the tumour samples (32.7%) were lacking in one transporter and only 11.2% possessed from three to five transporters. The canine transporter cBCRP was functionally analysed in stable transfected Madin-Darby canine kidney-II cells using an MTT viability test. cBCRP transfected cells showed a 5.4-fold resistance to 10 μ m doxorubicin. Cell survival in the presence of methotrexate was not affected by cBCRP. In conclusion, absence of efficiency of chemotherapy of canine mammary cancer can be caused by expression of seven various ABC-transport proteins. Because cBCRP is expressed in all examined tumour probes and induces resistance to doxorubicin, the application of doxorubicin for treatment of canine mammary is inappropriate.  相似文献   

Plasma vascular endothelial growth factor concentrations in healthy dogs and dogs with hemangiosarcoma     

Clifford CA  Hughes D  Beal MW  Mackin AJ  Henry CJ  Shofer FS  Sorenmo KU 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(2):131-135

Vascular endothelial growth factor (VEGF) is a dimeric glycosylated polypeptide growth factor with potent angiogenic, mitogenic, and vascular permeability-enhancing properties specific for endothelial cells. In humans, VEGF seems to play a major role in tumor growth, and plasma concentrations correlate with tumor burden, response to therapy, and disease progression. This study compared plasma VEGF concentrations in healthy client-owned dogs (n = 17) to dogs with hemangiosarcoma (HSA; n 16). Dogs with HSA were significantly more likely to have detectable concentrations of plasma VEGF (13/17) compared to healthy dogs (1/17; P < .001). The median plasma VEGF concentration for dogs with HSA was 17.2 pg/mL (range, < 1.0-66.7 pg/mL). Plasma VEGF concentrations in dogs with HSA did not correlate with stage of disease or tumor burden, but 1 dog had undetectable VEGF during chemotherapy that subsequently increased with disease progression.  相似文献   

Evaluation of body condition and weight loss in dogs presented to a veterinary oncology service     

Michel KE  Sorenmo K  Shofer FS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(5):692-695

Cancer cachexia is a well-recognized syndrome in human patients that is characterized by progressive involuntary weight loss. The prevalence of this syndrome in veterinary cancer patients is unknown. This study's objective was to investigate the occurrence of weight loss and cachexia, as characterized by body condition scoring, in dogs presented to a veterinary oncology service. Information collected on 100 dogs included signalment, diagnosis, weight at time of diagnosis, and, when available, weight from a time approximately 12 months before diagnosis. Body condition was assessed by using a 9-point system based on body silhouette and palpation of adipose tissue (4-5 = optimal, 1 = extreme cachexia, 9 = extreme obesity). Muscle wasting was scored based on palpation of skeletal muscle (3 = no wasting, 2 = mild, 1 = moderate, 0 = severe). Only 4% of the dogs exhibited cachexia as defined by a body condition score < or = 3, whereas 29% were classified as markedly overweight (> or = 7). Fifteen percent had evidence of clinically relevant muscle wasting (< or = 1). Body weight from a time before the diagnosis of cancer was available for 64 dogs. At the time of diagnosis, 31% had maintained or gained weight, 31% had lost up to 5%, 14% had lost between 5 and 10%, and 23% had lost >10% of body weight. Overall, the percentage of dogs with signs indicating a decline in nutritional status was less than what has been reported for human cancer patients. Future studies should investigate the extent to which weight loss occurs in canine patients on an appropriate plane of nutrition as well as to establish whether an association exists between poor nutritional status and outcome in canine cancer patients.  相似文献   

Canine hemangiosarcoma treated with standard chemotherapy and minocycline     

Sorenmo K  Duda L  Barber L  Cronin K  Sammarco C  Usborne A  Goldschmidt M  Shofer F 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(4):395-398

Standard treatments for canine hemangiosarcoma include surgery and chemotherapy with doxorubicin, but in spite of treatment most dogs with this disease die within 6 months of diagnosis. Tumor growth and metastasis are angiogenesis dependent. Antiangiogenic drugs such as minocycline may provide therapeutic benefits in cancer patients. The purpose of this prospective study was to evaluate the efficacy of chemotherapy with doxorubicin and minocycline, an antiangiogenic agent, in dogs with hemangiosarcoma. Eighteen dogs with histologically confirmed hemangiosarcoma of any stage were treated with doxorubicin, cyclophosphamide, and minocycline. Complete staging was performed before and during the treatment period to assess remission status and response to therapy. No statistically significant difference was found in survival between the dogs treated with chemotherapy and minocycline, and historical controls consisting of dogs that received chemotherapy alone. Postmortem examination revealed widespread metastasis, suggesting that minocycline is ineffective as a single antiangiogenic agent in canine hemangiosarcoma.  相似文献   

Distribution and genetic uniformity of an endangered aquatic plant, Ranunculus kadzusensis, in a South Korean rice paddy     

YOUN-BONG KU  HYUN KYUNG OH  JAE HO LEE  HAK YANG KONG  JI-HYON KIL  KANG-HYUN CHO 《Weed Biology and Management》2007,7(2):120-123

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Corrigendum to “A prospective investigation of the prevalence and prognostic significance of weight loss and changes in body condition in feline cancer patients” [Journal of Feline Medicine and Surgery 9 (2007) 411–417]     

Jennifer L. Baez VMD  Dipl ACVIM  SAIM  Oncology  Kathryn E. Michel DVM  Dipl ACVN  Karin Sorenmo CMV  Dipl ACVIM  Oncology  Frances S. Shofer PhD 《Journal of Feline Medicine and Surgery》2007,9(6):526

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浙江金华地区PCV2分子流行病学调查及遗传变异分析     

吴明臻  江云波  敖超杰  于学祥  库旭钢  陈芳洲  孙琪  吴昊  何启盖  赵青 《中国兽医学报》2019,(10):1890-1896

猪圆环病毒2型(porcine circovirus type 2,PCV2)主要引起猪的多系统功能障碍性疾病,同时,还可侵害猪的免疫系统,造成猪免疫抑制,容易继发细菌及其他病毒感染,大大增加猪群的死亡率。为了解PCV2在浙江金华猪群中的分布与流行状况以及PCV2变异规律,对从浙江省金华猪场收集的3 433份血清进行PCR检测,结果显示,2012-2018年PCV2阳性率分别为10.16%,11.95%,8.50%,9.94%,6.19%,5.94%,8.28%,母猪阳性率最高为13.49%。本研究发现在2012年浙江金华地区PCV2优势毒株已经由PCV2b变为PCV2d,且全部为PCV2d-2。将得到的ORF2序列同已知的疫苗序列对比后,发现SH株与浙江金华目前PCV2主流毒株序列一致性最高,但抗原位点比较仍有5处差异,这可能会对疫苗效果产生影响,需要进一步验证。另外只有PCV2d在免疫显性诱饵表位上发生了突变,这可能是PCV2d成为主流的原因。  相似文献   

引江济淮安徽段水污染物总量控制及水环境保护     

朱慧娈  匡武  吴蕾 《安徽农业科学》2016,44(30)

在对引江济淮规划区的概况、现状水质、现状污染源进行调查的基础上,进行了规划年2020年污染源预测。根据调水线路水质目标,选用合适的模型,以流域为单元,断面水质达标为目标,计算了引江济淮规划区各流域的纳污容量。在此基础上,制订了区域内的污染物总量控制方案,提出合理的水环境保护措施,确保引江济淮污染物削减目标可达,水质安全。  相似文献   

两种密度条件下玉米穗上节间距QTL分析   总被引：1，自引：1，他引：0  

任真真  周金龙  王小博  李国辉  朱宇光  库丽霞  陈彦惠 《玉米科学》2016,24(3):26-30

为研究高密度胁迫下穗上节间距的遗传机制,以豫82×沈137的重组近交系(RIL)群体为材料,构建一张包含有1 114个位点的SNP标记的遗传连锁图谱,图谱总长度为1 821.79 cM,标记平均间距为1.64 cM。利用复合区间作图法在60 000株/hm~2密度下共检测到11个、120 000株/hm~2密度下检测到12个穗上节间距的QTL,位于第6染色体上的QTLq For ILL6-2/qForILG6在两种密度下同时被检测到,表明该QTL均能稳定表达;位于第9染色体上的qThiILL9/qForILL9-1在60 000株/hm~2的密度下同时被检测到,解释穗上节间距遗传变异的8.67%和10.61%,在120 000株/hm~2密度下未被检测到,表明此QTL在低密度下特异表达调控穗上节间距3、4。在高密度下检测到qSixILG6和qSixILG10分别解释穗上节间距6遗传变异的16.97%和13.66%,说明该QTL在高密度环境下特异表达。  相似文献