Cloning and sequencing of the ovine gamma-interferon gene   总被引：1，自引：0，他引：1  

AJ RADFORD  ALM HODGSON  JS ROTHEL  PR WOOD 《Australian veterinary journal》1991,68(3):82-84

Cytokines are major modulators of the immune system of all animals. The cloning and expression of recombinant cytokine genes have permitted the analysis of their immune function and role in the control of the immune response to disease and vaccination. While human, murine, and bovine genes have been cloned and sequenced, the cloning of ovine cytokine genes has not yet been reported. As sheep are of dominant economic importance to the Australian farming industry, it is of significance to clone and express these genes to facilitate the development of new and better vaccines and pharmaceuticals. We have initially selected ovine gamma-interferon (gamma-IFN) as a target cytokine gene. By the use of the polymerase chain reaction (PCR), using primers based on the bovine gamma-interferon sequence, we have amplified the ovine gamma-interferon gene from crude messenger RNA extracted from lymphocytes. After cloning and DNA sequencing the gene, we found that ovine gamma-IFN is 93% identical to bovine gamma-IFN in amino acid sequence. This result indicates that the PCR method will be a rapid and efficient means for cloning other ovine cytokine genes.  相似文献   

Career aspirations of emerging veterinarians     

TJ HEATH  JS WESTERN 《Australian veterinary journal》1991,68(7):246-247

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Enhanced platelet reactivity in cats experimentally infected with feline infectious peritonitis virus     

M K Boudreaux  R C Weiss  M Toivio-Kinnucan  N Cox  J S Spano 《Veterinary pathology》1990,27(4):269-273

Platelet function was evaluated in six specific-pathogen-free cats prior to and following intraperitoneal inoculation with feline infectious peritonitis virus (FIPV). By 4 days post-inoculation, platelet samples from five of six cats responded with irreversible platelet aggregation to threshold concentrations of adenosine diphosphate (ADP). This was accompanied by enhanced platelet 14C-serotonin release (greater than 10%) in two cats. Compared to one of six baseline samples, five of five post-inoculation samples exhibited microaggregate formation in response to 20 microM epinephrine. Enhanced platelet 14C-serotonin release did not accompany these responses. Enhanced platelet responses to ADP and epinephrine were also observed on day 11 post-inoculation and day 16 (when one cat died) or 21 (the end of the study). Platelet 14C-serotonin release in response to 20 microM epinephrine increased markedly in three of five cats on day 21. Enhanced collagen-induced platelet responses were not demonstrated. Although the mechanism for the enhanced platelet responses observed on day 4 was unknown, a direct effect on the virus on platelets, mononuclear inflammatory cells, and endothelial cells must be considered.  相似文献   

Pathogenicity of Acholeplasma oculi for the sheep udder     

OP SADHANA  PP GUPTA  HS BANGA  JS RANA 《Australian veterinary journal》1993,70(6):227-228

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Development and trial of a bovine herpesvirus 1 - thymidine kinase deletion virus as a vaccine     

GA SMITH  PL YOUNG†  BJ RODWELL†  MA KELLY†  GJ STORIE†  CA FARRAH†  JS MATTICK† 《Australian veterinary journal》1994,71(3):65-70

SUMMARY An Australian bovine herpesvirus 1 (BHV1) isolate with a defined (427 base pair) deletion in the protein coding region of the thymidine kinase gene was obtained by standard marker rescue procedures. After selection in the presence of the nucleotide analogue 5iodo-deoxy-uridine the virus was analysed by hybridisation with three differential oligonucleotide probes, restriction endonuclease profile studies and DNA sequence analysis. The virus elicited an immune response in recipient animals after either intramuscular or intravenous administration and produced no significant deleterious side-effects when administered at a dose sufficient to stimulate the host immune response. The safety and immunogenicity of the recombinant BHV1 virus 39B1 were similar to those reported for other registered BHV1 vaccines and the virus would appear to be suitable for the production of a vaccine seed lot and more exhaustive field trials as a prelude to commercial vaccine production and registration.  相似文献   

Cutaneous botryomycosis in a crossbred domestic pig     

H Loader  KE Lawrence  N Brangenburg  JS Munday 《New Zealand veterinary journal》2018,66(4):216-218

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Cladosporium cladosporioides‐complex infection in a mixed‐breed dog          下载免费PDF全文

Matteo Spano  Davide Zuliani  Andrea Peano  Walter Bertazzolo 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(1):150-153

A 3‐year‐old female mixed‐breed dog was referred with a 2‐day history of serious dyspnea, coughing, lethargy, anorexia, and a low‐grade right anterior lameness. At presentation, the dog had an increased respiratory rate, dull heart and lung sounds, and cyanotic mucous membranes. It was hyperthermic and slightly dehydrated. Laboratory findings showed mild neutrophilia with a left shift, while serum biochemistry variables were in the normal range. However, urinalysis revealed mild proteinuria and rare erythrocytes and leukocytes on sediment examination. Thoracic radiographs showed a diffuse mixed interstitial and alveolar pattern with an air bronchogram, while appendicular radiographs showed a right humeral interrupted brush‐like periosteal reaction. Thoracic ultrasonography revealed mediastinal lymph node enlargement. Cytology from a fine‐needle aspirate of mediastinal lymph nodes revealed a pyogranulomatous lymphadenitis with numerous fungal hyphae. Culture on Sabouraud dextrose agar isolated dark fungal colonies with microscopic features consistent with Cladosporium spp. Sequencing of the internal transcribed spacer region identified the fungus as a species of the Cladosporium cladosporioides‐complex.  相似文献   

Phenotypic and genotypic profiling of antimicrobial resistance in enteric Escherichia coli communities isolated from finisher pigs in Australia          下载免费PDF全文

MG Smith  D Jordan  JS Gibson  RN Cobbold  TA Chapman  S Abraham  DJ Trott 《Australian veterinary journal》2016,94(10):371-376

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Using 20-gauge percutaneous peripheral catheters to reliably collect serial 4-mL blood samples from conscious dogs     

KF Elliott  LM Fleeman  JS Rand 《Australian veterinary journal》2010,88(6):215-221

Objective To determine the reliability of collecting serial 4-mL blood samples over 13 h using a 20-gauge, cephalic catheter in conscious dogs. Design Prospective, observational study. Animals Twelve (6 males, 6 females) healthy, neutered, lean dogs. Procedure Percutaneous placement of a 20-gauge, 1.1 × 30 mm intravenous over-the-needle catheter into the cephalic vein was performed in each dog on three occasions. Catheter patency was maintained by intermittent flushing with 0.9% saline and 1 IU/mL heparinised saline solutions. Blood samples (4 mL) were obtained at 10 time-points over 13 h, with close monitoring of the dogs and the indwelling catheters. Blood volume, resistance on aspirating blood sample (minimal or marked resistance) and the methods used to improve blood flow were recorded. Results A high proportion (93%) of blood sample collections achieved the required 4-mL volume, and the remaining samples were greater than 1.5 mL. Of the 358 collections via the cephalic catheter, 311 (87%) were obtained with ‘minimal resistance’. The remaining collections had ‘marked resistance’ (n=47) and were associated with a loose catheter in 11% (5/47) and of unknown cause in 89% (42/47). After ‘marked resistance’ had been encountered and the catheter was flushed with saline and heparin solutions, subsequent sampling with ‘minimal resistance’ was frequently possible from the same catheter. Conclusion Use of a percutaneous, 20-gauge intravenous cephalic catheter allowed reliable serial collection of 4-mL blood samples over 13 h in conscious dogs.  相似文献   

Is Mycoplasma bovis a missing component of the bovine respiratory disease complex in Australia?     

PF Horwood  ML Schibrowski  EV Fowler  JS Gibson  TS Barnes  TJ Mahony 《Australian veterinary journal》2014,92(6):185-191

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