Nitrogen deposition in Casuarina equisetifolia (Forst.) plantation stands in the dry tropics of Sonbhadra, India     

Alok K. Srivastava  R.S. Ambasht   《Forest Ecology and Management》1994,70(1-3):341-348

Periodic variations in the concentration, deposition and canopy impact of different forms of N on annual N deposition through rainfall, throughfall and stemflow in 5 and 8 year old stands of Casuarina equisetifolia were studied. Throughfall and stemflow ranged from 70 to 76% and 5–6% of annual precipitation respectively. The total N deposition by rainfall was 11.1 kg ha⁻¹ year⁻¹, and by throughfall was 13.6 kg ha⁻¹ year⁻¹ and 16.5 kg ha⁻¹ year⁻¹ in 5-year-old and 8-year old plantations, respectively. The quantities of N deposited through stemflow in the two plantations were nearly identical, accounting for 1.6 kg ha⁻¹ year⁻¹. Observations of the monthly deposition of NH₄,N, NO₃-N, Kjeldahl-N and organic-N revealed that maximum deposition occurred in July and the minimum in September. Organic-N deposition was 17% less (5-year) than the rainwater content. Net deposition of N, as an effect of canopy, was 7–8.7 kg ha⁻¹ year⁻¹, which was added directly to the available nutrient pool of soil.  相似文献   

Detection,Localization and Tyrosine Phosphorylation Status of Ser/Thr Protein Phosphatase1γ in Freshly Ejaculated,In Vitro Capacitated and Cryopreserved Buffalo Spermatozoa          下载免费PDF全文

S Naresh  SK Atreja 《Reproduction in domestic animals》2015,50(6):901-909

Several recent studies have indicated the important roles of Ser/Thr protein phosphatase1γ (PP1γ) in regulating the motility and capacitation of mammalian spermatozoa. Here, we report the presence and distribution of PP1γ protein in freshly ejaculated, in vitro capacitated and cryopreserved buffalo spermatozoa. The presence of PP1γ and its distribution were assessed by Western blotting and indirect immunofluorescence techniques, whereas the isoforms of PP1γ and their tyrosine phosphorylation status were identified by using 2D electrophoresis. The number of isoforms and the status of tyrosine phosphorylation of PP1γ were increased in capacitated spermatozoa when compared with freshly ejaculated spermatozoa. Differential pattern of expression and tyrosine phosphorylation of PP1γ were observed in cryopreserved spermatozoa, wherein some isoforms were degraded and some were tyrosine phosphorylated. In addition, immunofluorescence technique revealed that PP1γ was localized to principle, mid‐piece, post‐acrosomal and equatorial regions of buffalo spermatozoa. Differential distribution of tyrosine‐phosphorylated proteins were observed in fresh, capacitated and cryopreserved spermatozoa. The tyrosine phosphorylation of several proteins (20, 37, 38, 52, 60, 79 and 100 kDa) were increased when sperm cells were incubated with PP1γ inhibitor, okadaic acid. Together, our results suggest that buffalo spermatozoa express different isoforms of PP1γ protein. The protein expression and tyrosine phosphorylation of PP1γ were increased during capacitation. Furthermore, the differential pattern of expression and tyrosine phosphorylation of PP1γ were observed in cryopreserved spermatozoa. In addition, the inhibition of PP1γ protein increases protein tyrosine phosphorylation in capacitation.  相似文献   

Polygenetic Soils of the North-Central Part of the Gangetic Plains: A Micromorphological Approach   总被引：1，自引：0，他引：1  

P. Srivastava  B. Parkash 《CATENA》2002,46(4)

Micromorphology indicates that soils of the central part of the Gangetic Plains are polygenetic. They occur on surfaces originating at 13 500, 8000, 2500, >500 and <500 BP (QGH5 to QGH1, respectively). The QGH5 soils on upland interfluves show degraded illuvial clay pedofeatures of an early humid phase (13 500–11 000 BP) and thick (150–200 μm) microlaminated clay pedofeatures of a later humid phase (6500–4000 BP). The earlier clay pedofeatures were degraded by bleaching, loss of preferred orientation, development of a coarse speckled appearance and fragmentation, whereas those of the later phase are thick, smooth and strongly birefringent microlaminated clay pedofeatures. The illuviation was more extensive during the later phase, as indicated by enrichment of groundmass as discrete pedofeatures of clay intercalations. Pedogenic carbonate was formed during the intervening dry phase from the early Holocene to 6500 BP. It forms irregularly shaped nodules of micrite and diffuse needles with inclusions of soil constituents. The subsequent change to wetter conditions caused dissolution–reprecipitation, which resulted in partial to complete removal of carbonate from soils over large areas.  相似文献   

PH—Postharvest Technology: Physical Properties of Okra Seed     

P. K. Sahoo  A. P. Srivastava 《Biosystems Engineering》2002,83(4):441

The physical properties of okra seed were evaluated as a function of moisture content (m.c.). The average length, breadth and thickness of the seed varied from 5·92 to 7·30, 4·71 to 5·40 and 4·59 to 5·36 mm, respectively, as the moisture content increased from 8·16 to 87·57% d.b. The roundness and sphericity increased from 77·76 to 79·35% and 74·48 to 76·52%, respectively, with increase in moisture content from 8·16 to 19·56% d.b. and then decreased to 72·39 and 70·63%, respectively, with further increase of moisture content. In the moisture range of 8·16–87·57%, the seed volume increased from 0·067 to 0·124 cm³, 1000 seed weight, W₁₀₀₀ from 65·78 to 129·75 g and the angle of repose from 27·60 to 39·47°. The bulk density, true density and porosity decreased from 0·592 to 0·558 g cm⁻³, 1·107 to 0·986 g cm⁻³ and 46·34 to 43·20%, respectively, in the moisture range from 8·16 to 87·57% d.b. The static coefficient of friction increased on four structural surfaces namely, aluminium (0·390–0·484), bakelite (0·345–0·480), galvanised iron (0·368–0·493) and mild steel (0·389–0·480) as the moisture content increased from 8·16 to 87·57% d.b.  相似文献   

Rainwater Composition at a Regional Representative Site of a Semi-Arid Region of India     

Singh  S. P.  Khare  P.  Satsangi  G. S.  Lakhani  A.  Maharaj Kumari  K.  Srivastava  S. S. 《Water, air, and soil pollution》2001,127(1-4):93-108

Rainwater samples (N = 51) were collected at Rampur, an areafree from anthropogenic activity during the monsoon of 1997 and1998. The concentration of ions follows a general pattern as Ca> NH₄ > Mg > SO₄ > Cl > F >Na > NO₃ > K > HCOO >CH₃ COO. The pH of precipitation ranges between 5.9 and 7.4. The levels of Ca and Mg at this site are higher than otherremote sites, probably dominated by particles of soil origin.Good correlation between Ca, NO₃, SO₄, HCOO and CH₃COO indicate that a fraction of NO₃, SO₄, HCOOand CH₃COO may be derived from soil or associated with Ca and Mg after neutralization. The order of neutralization factorCa (2.19) > NH₄ (1.26) = Mg (1.26) indicates that majorneutralization occurred by Ca. Factor analysis suggested thatCa, Mg, Na, K, NO₃, SO₄, HCOO and CH₃COO arecontributed by soil. NH₃ is known to exist as(NH₄)₂SO₄, NH₄NO₃ and NH₄Cl. Theymay be formed in the atmospheric water droplets by scavenging ofaerosols and reaction of gaseous species.  相似文献   

Microbial contribution to extractable N and P after air-drying of dry tropical soils     

S. C. Srivastava 《Biology and Fertility of Soils》1997,26(1):31-34

The microbial contribution to extractable N and P after the air-drying of eight Indian dry tropical Ultisols was quantified. Air-drying of the soils decreased microbial biomass C by 25–53% but increased extractable N and P by 14–34% and 24–121%, respectively. This increase in the extractable N and P was accounted for, to some extent, by microbial biomass killed due to air-drying. Microbial biomass contributes 17–36% and 19–82% to the extractable N and P, respectively, possibly due to air-drying of the soils. I conclude that due to contamination of microbial biomass with the available nutrients in air-dried soils, measurements of extractable nutrients should be made on field-moist soils. Received: 22 October 1996  相似文献   

T-cell lymphoma associated with immunologic evidence of retrovirus infection in a lowland gorilla     

A W Prowten  R V Lee  R M Krishnamsetty  S K Satchidanand  B I Srivastava 《Journal of the American Veterinary Medical Association》1985,187(11):1280-1282

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Modified in vitro method to label equine red blood cells with technetium 99m in concentrated whole blood     

P D Koblik  W J Hornof  S C Srivastava 《American journal of veterinary research》1989,50(4):531-535

An in vitro method to label equine RBC with technetium 99m was modified to achieve quantitative labeling of cells in concentrated whole blood. After a blood sample was incubated with a reducing agent (stannous citrate), an oxidizing reagent (NaOCl) and a chelating agent (EDTA) were added to inactivate residual Sn2+ in the plasma. This step prevented premature reduction of pertechnetate in plasma. Labeling of RBC from 9 healthy horses, using a standard whole blood protocol, resulted in only moderate labeling efficiency (44 to 85%) and indicated a linear relationship between labeling efficiency and PCV. Effects of increased incubation time, increased incubation temperature, prelabeling sedimentation, and double addition of NaOCl/EDTA were investigated in whole blood from 10 healthy horses. Labeling efficiency was improved by each independent factor and by combination of factors. Highest labeling efficiencies (96 to 97%) were achieved when blood samples were sedimented for 20 minutes before being labeled, regardless of incubation time or incubation temperature. Morphologic features of RBC were unaffected by labeling procedures. In vivo whole blood clearance time for labeled cells was determined in 5 healthy horses. Sedimented blood samples were labeled, using a standard 15-minute incubation time at 20 to 22 C. Mean clearance half-time for 5 horses was approximately 20 hours. More than 95% of 99mTc activity was associated with the cells during the 24 hours after reinjection.  相似文献   

Treatment of "humpsore" in cattle caused by Stephanofilaria assamensis     

H D Srivastava  H C Malviya 《The Indian veterinary journal》1968,45(6):484-488

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Sensitive Detection of a Phytoplasma Associated with Little Leaf Symptoms in Withania somnifera     

J. A. Khan  P. Srivastava  S. K. Singh 《European journal of plant pathology / European Foundation for Plant Pathology》2006,115(4):401-408

Withania somnifera is an important medicinal plant native to the Indian-sub continent. Owing to the presence of a number of precious alkaloids, flavonoids and withanolides, it is widely used in the Indian and African systems of medicines. It is severely affected by phytoplasma present in the sieve tubes of phloem. With a view to micropropagate phytoplasma-free W. somnifera plants, an efficient and effective nested PCR-based system was developed for detection of associated phytoplasmas. Universal primers, designed from the 16S rDNA sequences of phytoplasmas, were applied in direct/nested-PCR. Total DNA extracts from leaf tissues of 33 suspected symptomatic and 11 non-symptomatic plants were subjected to direct PCR. The direct PCR products were subsequently employed as templates in nested PCR. The nested PCR could reamplify direct PCR products yielding a DNA fragment of 1.4 kb. A phytoplasma was detected in all the diseased plants and not from the healthy looking plants. Further, it was sensitive enough to amplify phytoplasma DNA obtained from crude DNA diluted up to 2500 times from naturally infected plants and also from various stages of in vitro-propagated diseased plants. Identical restriction fragment polymorphism enzyme profiles were obtained following restriction enzyme digestion of nested PCR products, obtained from five different plants, by EcoRI, AluI and RsaI restriction endonucleases. The developed nested PCR based system should facilitate indexing of the phytoplasma in different stages of in vitro-generated plants and probably identification of, as yet unknown, hosts and vectors of phytoplasma associated with phytoplasma disease of W. somnifera.  相似文献