A molecular pathway revealing a genetic basis for human cardiac and craniofacial defects     

Yamagishi H  Garg V  Matsuoka R  Thomas T  Srivastava D 《Science (New York, N.Y.)》1999,283(5405):1158-1161

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An inhibitor of FtsZ with potent and selective anti-staphylococcal activity     

Haydon DJ  Stokes NR  Ure R  Galbraith G  Bennett JM  Brown DR  Baker PJ  Barynin VV  Rice DW  Sedelnikova SE  Heal JR  Sheridan JM  Aiwale ST  Chauhan PK  Srivastava A  Taneja A  Collins I  Errington J  Czaplewski LG 《Science (New York, N.Y.)》2008,321(5896):1673-1675

FtsZ is an essential bacterial guanosine triphosphatase and homolog of mammalian beta-tubulin that polymerizes and assembles into a ring to initiate cell division. We have created a class of small synthetic antibacterials, exemplified by PC190723, which inhibits FtsZ and prevents cell division. PC190723 has potent and selective in vitro bactericidal activity against staphylococci, including methicillin- and multi-drug-resistant Staphylococcus aureus. The putative inhibitor-binding site of PC190723 was mapped to a region of FtsZ that is analogous to the Taxol-binding site of tubulin. PC190723 was efficacious in an in vivo model of infection, curing mice infected with a lethal dose of S. aureus. The data validate FtsZ as a target for antibacterial intervention and identify PC190723 as suitable for optimization into a new anti-staphylococcal therapy.  相似文献   

Screening potato cultivars for common scab of potato in a naturally infested field     

K. K. Mishra  J. S. Srivastava 《Potato Research》2001,44(1):19-24

Summary Twenty seven cultivars of potato were screened for common scab grown in a commercial field in two successive years (1996–97 and 1997–98). Eight cultivars were least susceptible and the others ranged from medium susceptible to very highly susceptible. None of the cultivars was resistant. Most showed a stable resistance reaction in both years.  相似文献   

Sesbania aculeata leaf meal as replacer of de‐oiled rice bran in aquaculture feed: Growth,IGF‐1 expression,metabolic and biochemical responses in Cyprinus carpio (Linnaeus 1758)     

Garima Anand  Prem Prakash Srivastava  Tincy Varghese  Narottam P. Sahu  Vungarala Harikriskna  Martin Xavier  Iffat Jahan  Dharmaraj Patro 《Aquaculture Research》2020,51(6):2483-2494

A feeding trial was conducted to evaluate the effect of inclusion of Sesbania leaf meal in the diet of Cyprinus carpio as a replacer of de‐oiled rice bran (DORB). Bacillus subtilis was used to ferment the Sesbania leaf meal (SLM) to produce FSLM (fermented sesbania leaf meal). Fishes were fed with five isonitrogenous (30% CP) and isocaloric diets for 60 days and treatments were viz., C (30% DORB, 0% SLM), R15 (15% DORB, 15% SLM), R30 (0% DORB, 30% SLM), F15 (15% DORB, 15%FSLM) and F30 (0% DORB, 30% FSLM) by replacing DORB with SLM. A similar growth performance with control was observed in 15% FSLM group while there was a growth reduction in all the raw leaf meal fed groups. Similarly, the expression of insulin‐like growth factor‐1 (IGF‐1) was significantly (p < .05) higher in control and 15% FSLM fed groups. Growth and metabolic enzymes such as protease, amylase, ALT and AST activities were significantly higher in the fermented leaf meal fed groups compared to their non‐fermented counterparts. Antioxidant enzymes, serum transaminase enzymes (SGOT, SGPT) and total bilirubin (TBR) were found to be increased by enhanced level of RSLM, however, reduced by inclusion of FSLM. However, 30% inclusion level of FSLM even after fermentation showed detrimental effect on growth and metabolic response at cellular and molecular level. Thus, the results concluded that fermented Sesbania leaf meal (FSLM) using B. subtilis could replace 50% DORB (15% FSLM group) in the diet of C. carpio without affecting the growth and other physiological responses.  相似文献   

Identification and characterization of SSRs in <Emphasis Type="Italic">Clarias batrachus</Emphasis> and their application in population study     

Shreya Srivastava  Basdeo Kushwaha  Jyoti Prakash  Manmohan Pandey  Suyash Agarwal  Ravindra Kumar  N. S. Nagpure  Mahender Singh  Paramananda Das  Chaitanya Joshi  Joy Krushna Jena 《Fisheries Science》2017,83(2):265-272

Magur Clarias batrachus is an indigenous catfish, commonly found in India, Nepal, Bhutan and Bangladesh. Presently, the populations of magur have declined rapidly in their natural habitat mainly due to overexploitation and habitat degradation. Understanding the population genetic structure of the species has significance in improvement of stocks and in conservation of the species. In the present study, simple sequence repeat (SSR) markers were used to differentiate the populations of magur, collected from three geographic locations. For this, a total of 31,814 SSRs were mined from the de novo assembled pooled of whole genome sequence data of C. batrachus. A bioinformatics pipeline with stringent criteria was applied to analyze the data which resulted in selection of 30,142 microsatellite loci falling in the intergenic region. Out of the 25 loci selected for primer development, 16 loci were successfully amplified and 9 loci were found to be polymorphic in this species. The average observed as well as expected heterozygosity in the loci across different stocks varied from 0.652 to 0.688 and 0.864 to 0.873, respectively. These three populations were further segregated into two clusters based on the NJ genetic distance. The Lucknow population formed one cluster, while the Bhubaneswar and Kolkata populations constituted another cluster. A comparable finding was also deduced from the STRUCTURE analyses. The results revealed significant variation among the populations of C. batrachus under study.  相似文献   

Morpho-physiological evaluation of Elymus semicostatus (Nees ex Steud.) Melderis as potential donor for drought tolerance in Wheat (Triticum aestivum L.)     

Kumar  Antul  Sharma  Achla  Sharma  Rajni  Choudhary  Anuj  Srivastava  Puja  Kaur  Harmanjot  Padhy  Asish Kumar 《Genetic Resources and Crop Evolution》2022,69(1):411-430

Genetic Resources and Crop Evolution - Gradual deterioration in water resources and unpredictable distribution pattern of annual precipitation amount are major threats having drastic effects on...  相似文献