Plasma pharmacokinetics and milk levels of ceftriaxone following single intravenous administration in healthy and endometritic cows     

Sudershan Kumar  Anil K. Srivastava  V. K. Dumka  Naresh Kumar  Rajinder K. Raina 《Veterinary research communications》2010,34(6):503-510

Pharmacokinetics and milk levels of ceftriaxone were studied in healthy and endometritic cows following single intravenous administration. The drug was detected up to 8 h of dosing in plasma of healthy and endometritic cows and the drug disposition followed three-compartment open model. The values of Vd_area, AUC, t_1/2β, Cl_B, MRT and P/C ratio were 0.50 ± 0.19 L.kg⁻¹, 62.2 ± 23.3 μg.ml⁻¹.h, 1.02 ± 0.07 h, 0.30 ± 0.09 L.kg⁻¹.h⁻¹, 1.55 ± 0.25 h and 0.52 ± 0.27, respectively, in healthy and 1.55 ± 0.52 L.kg⁻¹, 37.0 ± 17.1 μg.ml⁻¹.h, 1.56 ± 0.25 h, 0.56 ± 0.14 L.kg⁻¹.h⁻¹, 2.14 ± 0.34 h and 1.44 ± 0.60, respectively, in endometritic cows. The drug was detected in milk for 36 h after administration. For MIC₉₀ of 0.5 μg.ml⁻¹ the most appropriate dosage for ceftriaxone, would be 9.0 mg.kg⁻¹ repeated at 6 h intervals for the treatment of endometritis in cows.  相似文献   

Phenolic glycosides from Dodecadenia grandiflora and their glucose-6-phosphatase inhibitory activity     

Manmeet Kumar  Preeti Rawat  Mohammad Faheem Khan  Akhilesh K. Tamarkar  Arvind K. Srivastava  Kamal R. Arya  Rakesh Maurya 《Fitoterapia》2010

Phytochemical investigation of Dodecadenia grandiflora leaves led to the isolation and identification of three phenolic glycosides, designated 1-[(4′-O-(E)-p-coumaroyl)-β-d-glucopyranosyl]-oxy-2-phenol (1), 1-[(6′-O-(E)-p-coumaroyl)-β-d-glucopyranosyl]-oxy-2-phenol (2) and 1-[O-β-d-glucopyranosyl(1→2)-β-d-glucopyranosyl]-oxy-2-phenol (3), along with nine known compounds. Compounds 1, 2, 5 and 9 exhibited significant glucose-6-phosphatase inhibitory activity (63.7, 66.9, 82.9 and 85.4%) with IC₅₀ values of 88.5, 81.0, 51 and 50 μM respectively. On the basis of biological results, a structure–activity relationship has been discussed.  相似文献   

Exogenous proline alleviates oxidative stress and increase vase life in rose (Rosa hybrida L. ‘Grand Gala’)     

Naveen Kumar  Madan PalAtar Singh  Raj Kumar SaiRamGirish Chand Srivastava 《Scientia Horticulturae》2010

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Physiological Studies During the Growth and Development of Different Varieties of Guavas (Psidium Guajava L.)     

H.C. Srivastava  P. Narasimhan 《The Journal of Horticultural Science and Biotechnology》2013,88(1):97-104

In a study of three seed-bearing varieties of guava and one seedless variety changes in size and specific gravity and in the content of water, titratable acid, reducing and non-reducing sugars, tannins, pectin, and ascorbic acid were recorded at intervals from fruit-set to harvest. Respiration rates were also determined during this period.

Patterns of change were generally similar in seeded and unseeded varieties, though the sugars and ascorbic acid contents rose earlier and to higher levels in the seedless than in the seeded, and the fruits matured and fell from the trees earlier.

Increasing ascorbic acid content, concurrent with declining pectin content in the seeded varieties, is consistent with the hypothesis that pectin degradation and ascorbic acid are linked, but in the seedless variety a rapid rise in the ascorbic acid content occurred during a period of pectin accumulation. The end of the initial rapid decline in respiration rate generally coincided with the beginning of reducing-sugar accumulation.  相似文献   

Response of cornmint (Mentha arvensis L. f. piperascens Malinv. ex Holmes) to micronutrients     

D. K. Rajput  P. C. Srivastava 《The Journal of Horticultural Science and Biotechnology》2013,88(4):438-440

Summary

A field experiment was carried over two years (1999 and 2000) in the subtropical climate of north India in a mollisol, to study the response of cornmint (Mentha arvensis L. f. piperascens Malinv. ex Holmes, Lamiaceae) ‘Himalaya ’ to application of six micronutrients, namely, iron (Fe: 15, 30, 45 kg ha^–1) manganese (Mn: 10, 20, 30 kg ha^–1), zinc (5, 10, 15 kg ha^–1), copper (Cu: 2, 4, 6 kg ha^–1), boron (B: 1, 2, 3 kg ha^–1) and molybdenum (Mo: 0.05, 0.10, 0.20 kg ha^–1). Application of micronutrients significantly increased the essential oil yield of cornmint in comparison with the control. However, the response to individual applications of micronutrients was observed up to 15 kg Fe, 10 kg Mn, 10 kg Zn, 2 kg Cu, 2 kg B or 0.05 kg Mo ha^–1. Beyond these levels, the essential oil yield variations among the doses within each trace element were not statistically significant. Of these rates of application, 2 kg B produced the maximum essential oil yield (116.5 kg ha^–1). The percentage increases in the essential oil yield over the control were: 36.2 for 15 kg Fe, 47.3 for 10 kg Mn, 35.7 for 10 kg Zn, 23.9 for 2 kg Cu, 62.9 for 2 kg B and 33.4 for 0.05 kg Mo.  相似文献   

A PCR-based assessment of genetic diversity,and parentage analysis among commercial mango cultivars and hybrids     

Anuj P. Srivastava  Ramesh Chandra  Sangeeta Saxena  Shailendra Rajan  Shirish A. Ranade 《The Journal of Horticultural Science and Biotechnology》2013,88(6):951-959

Summary

Three different PCR methods [Random Amplified Polymorphic DNA (RAPD), Inter-Simple Sequence Repeats (ISSR), and Directed Amplification of Minisatellite DNA (DAMD)] were used to analyse genetic diversity and parentage among 20 mango cultivars, including 18 landraces and two hybrids (‘Amrapali’ and ‘Mallika’). These hybrids together with a third hybrid (‘Ratna’), and an out-group species (Mangifera sylvatica) were also analysed for parentage. Fifteen, seven and four primers were used to amplify a total of 158, 69 and 59 distinct DNA fragments by RAPD, ISSR and DAMD, respectively. Of these, approx. 85%, 64% and 90% were polymorphic, respectively. Genetic distances between pairs of mango cultivars were measured separately by each method and depicted graphically as a Neighbor Joining (NJ) tree. The three methods revealed different groupings of cultivars and hybrids. A NJ tree based on the cumulative data from all methods correlated well with the parentage of the mango hybrids, and the grouping of cultivars on a regional basis. Genetic markers likely to be associated with important agronomic traits were identified by further analysing the hybrids, with their respective parents, using all three methods. On the basis of the highest number of polymorphic bands observed (90%), DAMD was judged to be the best method with which to analyse mango germplasm.  相似文献   

Identification of unique DNA sequences present in highly virulent 2009 Alabama isolates of Aeromonas hydrophila     

Pridgeon JW  Klesius PH  Mu X  Carter D  Fleming K  Xu D  Srivastava K  Reddy G 《Veterinary microbiology》2011,152(1-2):117-125

In 2009, a disease outbreak caused by Aeromonas hydrophila occurred in 48 catfish farms in West Alabama, causing an estimated loss of more than 3 million pounds of food size channel catfish. Virulence studies have revealed that the 2009 isolates of A. hydrophila are at least 200-fold more virulent than a 1998 Alabama isolate AL98-C1B. However, up to now, no molecular markers have been identified to differentiate the highly virulent 2009 isolates from other isolates of A. hydrophila. To understand the genetic differences between the highly virulent 2009 isolates and the less virulent AL98-C1B at molecular level, PCR-select bacterial genome subtractive hybridization was used in this study. A total of 96 clones were selected from the subtractive genomic DNA library. Sequencing results revealed that the 96 clones represented 64 unique A. hydrophila sequences. Of the 64 sequences, three (hypothetical protein XAUC_13870, structural toxin protein RtxA, and putative methyltransferase) were confirmed to be present in the three virulent 2009 Alabama isolates but absent in the less virulent AL98-C1B. Using genomic DNAs from nine field isolates of A. hydrophila with different virulence as templates, two sequences (hypothetical protein XAUC_13870 and putative methyltransferase) were found to be only present in highly virulent A. hydrophila isolates, but absent in avirulent isolates.  相似文献   

Zinc Nutrition in ‘Nagpur’ Mandarin on Haplustert     

A. K. Srivastava  Shyam Singh 《Journal of plant nutrition》2013,36(7):1065-1081

ABSTRACT

Zinc (Zn) deficiency is the most prevalent nutritional disorder in citrus orchards world over. The management strategy of Zn deficiency today is still governed by the efficacy of two conventionally used methods of Zn supply to plants via soil or foliar fertilization. A field experiment with 12-yr-old ‘Nagpur’ mandarin (Citrus reticulata Blanco) orchard was, therefore, carried out during 2004–07 comparing soil application versus foliar application of Zn, each at three levels viz., 100, 200, and 300 g tree^?1 with constant doses of N (600 g tree^?1), P (200 g tree^?1), K (300 g tree^?1), and Fe(60 g tree^?1) on Haplustert soil type with reference to response on flowering intensity, fruit set, tree volume, fruit yield, changes in soil fertility/leaf nutrient status, fruit quality, and transformation of native soil Zn fractions. Soil application of Zn at all the three levels, produced significantly higher increase in tree volume over foliar application on equivalent rates viz., T₁ (2.53 m³) vs. T₄ (2.06 m³) and T₂(4.30 m³) vs. T₅ (2.23 m³). The yield-determining parameters like flowering and fruit set intensity (no. m^?1 shoot length) were, respectively, much higher with soil applied (135.74 and 21.90) than foliar applied Zn (31.20 and 11.6). These observations set the favorable conditions required for yield response, e.g., all the three treatments involving soil application of Zn, T₁ (32.1 kg tree^?1), T₂ (52.6 kg tree^?1), and T₃ (51.8 kg tree^?1) were correspondingly superior over T₄ (22.5 kg tree^?1), T₅ (34.3 kg tree^?1), and T₆ (42.1 kg tree^?1) as foliar application treatments. All the three major fruit quality parameters (juice, acidity, and TSS) were likewise more influenced by soil application than foliar application of Zn. Improvements in soil Zn fractions (mg kg^?1) viz., exchangeable Zn (0.25–0.60), complex-Zn (2.71 to 4.86), organically bound Zn (0.86 to 2.0), and Zn-bound to carbonates and acid soluble minerals (2.56–4.96) were observed in response to Zn fertilization with treatments T₁–T_3. On the other hand, foliar applied Zn treatments (T₄–T₆) produced no such changes in any of the soil Zn fractions.  相似文献   

An analysis of the persistence and potency of film‐coated seed protectant as influenced by various storage parameters     

Sherry Rachel Jacob  MB Arunkumar  Madhuban Gopal  Chitra Srivastava  SN Sinha 《Pest management science》2009,65(7):817-822

BACKGROUND: An efficient delivery system for seed‐protectant chemicals is needed in light of several disadvantages of conventional seed treatment methods. This study evaluates the efficacy of film‐coat application in maintaining the persistence and potency of imidacloprid on Lycopersicon esculentum (L.) Mill. seeds after simultaneous storage under ambient and regulated environment in paper and aluminium packages. RESULTS: High‐performance liquid chromatography (HPLC) revealed 0.135 mg kg^?1 of herbage material to be the threshold value beyond which absolute control was obtained, and with film coating the latter was achieved even with half‐dosage seed treatment, irrespective of the storage condition. The technique provided early protection to the crop and also nullified the deleterious effects of ambient storage on the persistence and potency of the pesticide. CONCLUSION: Film coating enabled superior pesticide dosage as well as higher biological efficacy to be achieved. Hence, in addition to being an ecofriendly alternative, the technique would be a more economically viable option for storage of treated seeds. Copyright © 2009 Society of Chemical Industry  相似文献   

Physiological and Biochemical Responses of Hexaploid and Tetraploid Wheat to Drought Stress   总被引：8，自引：1，他引：7  

V. Chandrasekar  R. K. Sairam  G. C. Srivastava 《Journal of Agronomy and Crop Science》2000,185(4):219-227

An experiment was conducted to investigate the physiological and biochemical responses of two hexaploids viz., C 306 (water stress tolerant) and Hira (water stress susceptible), and two tetraploids, HW 24 (Triticum dicoccum) and A 9‐30‐1 (Triticum durum) wheat genotypes to water stress under pot culture condition. Water stress was imposed for a uniform period of 10 days at 50, 60 and 70 days after sowing (DAS) and observations were recorded at 60, 70 and 80 DAS. Total dry matter and plant height were recorded at harvest. Water stress caused a decline in relative water content (RWC), chlorophyll and carotenoid content, membrane stability and nitrate reductase activity and increased accumulation of proline at all stages and abscisic acid (ABA) at 80 DAS in all the genotypes. Both the tetraploids showed a lower reduction in RWC and highest ABA accumulation under water stress. Among the hexaploids Hira showed the most decline in RWC and the lowest ABA accumulation. The tetraploids also showed comparatively higher carotenoid content and membrane stability, closely followed by C 306, while Hira showed the minimum response under water stress. Nitrate reductase activity and chlorophyll content under irrigated conditions were highest in Hira but under water stress the lowest per cent decline was observed in C 306, followed by HW 24, A 9‐30‐1, and Hira. Proline accumulation under water stress conditions was highest in hexaploids C 306 and Hira and lowest in tetraploids HW 24 and A 9‐30‐1. Tetraploids HW 24, followed by A 9‐30‐1 maintained higher plant height and total dry matter (TDM) under water stress and also showed a lower per cent decline under stress than hexaploids C 306 and Hira. From the results it is clear that proline accumulation did not contribute to better drought tolerance of tetraploids than hexaploids. It is also apparent that water stress tolerance is the result of the cumulative action of various physiological processes, and all the parameters/processes may not be positively associated with the drought tolerance of a particular tolerant genotype.  相似文献