Effects of iodine methionine on boar sperm quality during liquid storage at 17°C          下载免费PDF全文

Q Fang  J Wang  YY Hao  H Li  JX Hu  GS Yang  JH Hu 《Reproduction in domestic animals》2017,52(6):1061-1066

Microbial environment is one of the important factors that affect the quality of preserved semen. Iodine methionine (IM), participating in the production and activation of metabolic enzymes, is a new type of amino acid chelate. To date, there has been no report to evaluate the effects of IM on boar semen preservation at 17°C. This study was designed to investigate the effects of IM on boar sperm quality and reproductive performance during liquid storage at 17°C and its antibacterial effect. Semen samples collected from six Yorkshire boars were diluted with basic liquid containing different concentrations of IM (0, 20, 40, 80, 160 and 320 μM). Subsequently, sperm motility, plasma membrane integrity and acrosome integrity were determined. After 6 days of preservation, the difference in microbial composition between control group and 80 μM IM group was compared using 16S rDNA sequencing, and the effects of IM on reproductive performance were also compared and analysed between the two groups. The results demonstrated that 20, 40 and 80 μM IM improved boar sperm motility, plasma membrane integrity and acrosome integrity. 80 μM IM was the optimum concentration. Conversely, 160 and 320 μM IM resulted in deleterious consequences to boar sperm quality compared to the control group and other treatment groups (p < .05). After 6 days of preservation, sperm motility, plasma membrane integrity and acrosome integrity were 56.0%, 51.8% and 59.4%, respectively. There was no significant difference in non‐return rate between the two groups (p > .05). But the litter size of 80 μM IM group was significantly higher than that of control group (p < .05). 80 μM IM inhibited proliferation of the phylum Proteobacteria and the genus Staphylococcus as well as Pseudomonas (p < .05). Further studies are required to understand the antibacterial mechanism of IM in liquid‐preserved boar semen.  相似文献   

Pregnancy Rates to Fixed Embryo Transfer of Vitrified IVP Bos indicus,Bos taurus or Bos indicus × Bos taurus Embryos          下载免费PDF全文

LSR Marinho  BV Sanches  CO Rosa  JH Tannura  AG Rigo  AC Basso  JHF Pontes  MM Seneda 《Reproduction in domestic animals》2015,50(5):807-811

The pregnancy rates obtained after the transfer of cryopreserved in vitro‐produced (IVP) embryos are usually low and/or inconsistent. The objective of this study was to evaluate the pregnancy rates of Holstein, Gyr and Holstein × Gyr cattle after the transfer of vitrified IVP embryos produced with X‐sorted sperm. Seventy‐two Gyr and 703 Holstein females were subjected to ovum pickup (OPU) sessions, followed by in vitro embryo production using semen from sires of the same breeds. Embryos (1636 Holstein, 241 Gyr and 1515 Holstein × Gyr) were exposed to forskolin for 48 h prior to vitrification. The pregnancy rate achieved with Gyr dam and sire was 46.1%, which was similar (p = 0.11) to that of Holstein dam and Gyr sire (40.3%). Crossing Gyr dams with Holstein sires resulted in a pregnancy rate of 38.9% and did not differ (p = 0.58) from the pregnancy rate obtained with the cross between Holstein dams and Gyr sires. The rate obtained with Holstein dam and sire was 32.5%. The average pregnancy rate was 36.6%, and no difference was found in the proportion of female foetuses (88.8%, in average) among breeds (p > 0.05). In conclusion, transfer of cryopreserved X‐sorted embryos represents an interesting choice for dairy cattle. Despite the small differences between pregnancy rates, we highlight the efficiency of this strategy for all of the racial groups studied.  相似文献   

Impacts of rising atmospheric carbon dioxide on model terrestrial ecosystems   总被引：2，自引：0，他引：2  

TH Jones  LJ Thompson  JH Lawton  TM Bezemer  RD Bardgett  TM Blackburn  KD Bruce  PF Cannon  GS Hall  SE Hartley  G Howson  CG Jones  C Kampichler  E Kandeler  DA Ritchie 《Science (New York, N.Y.)》1998,280(5362):441-443

In model terrestrial ecosystems maintained for three plant generations at elevated concentrations of atmospheric carbon dioxide, increases in photosynthetically fixed carbon were allocated below ground, raising concentrations of dissolved organic carbon in soil. These effects were then transmitted up the decomposer food chain. Soil microbial biomass was unaffected, but the composition of soil fungal species changed, with increases in rates of cellulose decomposition. There were also changes in the abundance and species composition of Collembola, fungal-feeding arthropods. These results have implications for long-term feedback processes in soil ecosystems that are subject to rising global atmospheric carbon dioxide concentrations.  相似文献   

Alexander Kennedy Sutherland, OAM     

KL Hughes  JH Arundel 《Australian veterinary journal》2000,78(1):58-58

  相似文献   

Estimating degradation rates in outdoor stagnant water by inverse modelling with TOXSWA: a case study with prosulfocarb     

Paulien I Adriaanse  Jos JTI Boesten  Steven JH Crum 《Pest management science》2013,69(6):755-767

  相似文献   

Haemolytic anaemia associated with Theileria sp. in an orphaned platypus     

AE Kessell  JG Boulton  GJ Dutton  R Woodgate  S Shamsi  A Peters  JH Connolly 《Australian veterinary journal》2014,92(11):443-449

  相似文献   

Terminology of Deer     

JH Arundel 《Australian veterinary journal》1996,73(1):39-39

  相似文献   

Cerebral coenuriasis in a domestic cat     

RF SLOCOMBE  JH ARUNDEL  R. LABUC  MK DOYLE 《Australian veterinary journal》1989,66(3):92-93

  相似文献   

miRNA and piRNA expression profiles of breeder cock testes detected by next‐generation sequencing          下载免费PDF全文

SR Wu  W Guo  YL Li  XC Ren  XY Lei  XY Li  JH Yao  XJ Yang 《Reproduction in domestic animals》2017,52(2):203-213

miRNAs are small non‐coding regulatory RNAs that play key roles in diverse biological processes. In this study, we used the Solexa sequencing technique to profile miRNAs in breeder cock testes to illustrate their functions. A total of 663 co‐expressed miRNAs and 3,180 co‐expressed piRNAs were detected in three libraries. Based on Mir‐X^? miRNA qRT‐PCR, three miRNAs representing low, medium and high expression levels according to the sequencing results were selected randomly to validate the miRNAs' expression profiles. Results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, target genes prediction of the co‐expressed miRNAs and further Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed, which revealed that some candidate miRNAs were involved in the regulation of the spermatogenesis process, spermatozoa function and testicular metabolism. In conclusion, we provided a useful resource for further elucidation of the miRNAs' regulatory role in spermatogenesis, contributing to a preliminary database for functional and molecular mechanistic studies in testicular metabolism, spermatogenesis and other testes functions.  相似文献   

Demographic and preliminary employment data of the first two graduate cohorts from a rural veterinary school          下载免费PDF全文

JH Hyams  SL Raidal  LM Hayes  J Heller 《Australian veterinary journal》2017,95(4):91-100

  相似文献