Localization of Insulin‐Like Growth Factor‐I (IGF‐I) and IGF‐I Receptor (IGF‐IR) in Equine Testes     

MJ Yoon  T Berger  JF Roser 《Reproduction in domestic animals》2011,46(2):221-228

The insulin‐like growth factor‐I (IGF‐I) is a key regulator of reproductive functions. IGF‐I actions are primarily mediated by IGF‐IR. The main objective of this research was to evaluate the presence of IGF‐I and IGF‐I Receptor (IGF‐IR) in stallion testicular tissue. The hypotheses of this study were (i) IGF‐I and IGF‐IR are present in stallion testicular cells including Leydig, Sertoli, and developing germ cells, and (ii) the immunolabelling of IGF‐I and IGF‐IR varies with age. Testicular tissues from groups of 4 stallions in different developmental ages were used. Rabbit anti‐human polyclonal antibodies against IGF‐I and IGF‐IR were used as primary antibodies for immunohistochemistry and Western blot. At the pre‐pubertal and pubertal stages, IGF‐I immunolabelling was present in spermatogonia and Leydig cells. At post‐pubertal, adult and aged stages, immunolabelling of IGF‐I was observed in spermatogenic cells (spermatogonia, spermatocyte, spermatid, and spermatozoa) and Leydig cells. Immunolabelling of IGF‐IR was observed in spermatogonia and Leydig cells at the pre‐pubertal stage. The immunolabelling becomes stronger as the age of animals advance through the post‐pubertal stage. Strong immunolabelling of IGF‐IR was observed in spermatogonia and Leydig cells at post‐puberty, adult and aged stallions; and faint labelling was seen in spermatocytes at these ages. Immunolabelling of IGF‐I and IGF‐IR was not observed in Sertoli cells. In conclusion, IGF‐I is localized in equine spermatogenic and Leydig cells, and IGF‐IR is present in spermatogonia, spermatocytes and Leydig cells, suggesting that the IGF‐I may be involved in equine spermatogenesis and Leydig cell function as a paracrine/autocrine factor.  相似文献   

Effects of Superoxide Disrnutase and Allopurinol on the Survival of Acute Island Skin Flaps     

Im MJ  Manson PN  Bulkely GB  Hooper JE 《Veterinary surgery : VS》1985,14(3):234-234

  相似文献   

Flood plain staggers, a tunicaminyluracil toxicosis of cattle in northern New South Wales     

CA BOURKE  MJ CARRIGAN  SCJ LOVE 《Australian veterinary journal》1992,69(9):228-229

  相似文献   

An outbreak of listerial myelitis in sheep     

JT SEAMAN  MJ CARRIGAN  FA COCKRAM  GI CARTER 《Australian veterinary journal》1990,67(4):142-143

  相似文献   

A review of cases submitted to Murdoch University for immunodiagnostic testing: 1978–1989     

WJ PENHALE  MJ DAY†  AD LINES  RP McKENNA 《Australian veterinary journal》1990,67(4):148-149

  相似文献   

Experimental evidence that tryptamine alkaloids do not cause Phalaris aquatica sudden death syndrome in sheep     

CA BOURKE  MJ CARRIGAN  RJ DIXON† 《Australian veterinary journal》1988,65(7):218-220

The acute toxicity for sheep of 3 alkaloids that occur in Phalaris acquatica was examined by intravenous and oral administration. The lowest tested dose rates that produced clinically observed signs were, for 5-methoxy dimethyltryptamine, 0.1 mg/kg body weight intravenously and 40 mg/kg orally; for gramine, 10 mg/kg intravenously and 500 mg/kg orally; and for hordenine, 20 mg/kg intravenously and 800 mg/kg orally. All induced the clinical signs observed in the nervous form of phalaris toxicity, but none induced the cardiac, sudden death, syndrome.  相似文献   

Chronic locomotor dysfunction, associated with a thalamic-cerebellar neuropathy, in Australian Merino sheep     

CA BOURKE  MJ CARRIGAN  CHR DENT 《Australian veterinary journal》1993,70(6):232-233

  相似文献   

A brief review of studies of bovine and equine herpesviruses     

MJ STUDDERT 《Australian veterinary journal》1989,66(12):401-402

  相似文献   

Vaccine efficacy for reducing turbinate atrophy and improving growth rate in piggeries with endemic atrophic rhinitis     

MJ KABAY  AR MERCY  JM LLOYD  GM ROBERTSON 《Australian veterinary journal》1992,69(5):101-103

Two vaccines, based on formalin-killed whole cells of toxigenic Pasteurella multocida type D and Bordetella bronchiseptica combined with a partially toxoided cell extract of P multocida, were prepared with Freund's incomplete adjuvant (vaccine 1) or by alum precipitation (vaccine 2). Each was tested for safety and efficacy in reducing the severity of nasal turbinate atrophy and improving the growth rate of pigs in three Western Australian commercial piggeries with endemic atrophic rhinitis. In safety experiments with vaccine 1, no adverse clinical effects were observed in vaccinated sows or their progeny. Piglets receiving vaccine 2 showed no injection site abnormalities, pyrexia or turbinate atrophy. In field trials, vaccine 1 significantly reduced the prevalence of moderate to severe nasal turbinate atrophy (Done score 3 to 5) when used in two piggeries (A and B). Progeny from vaccinated sows in piggery B also grew significantly faster than controls. When vaccine 2 was used in piggery A at a later date and in another piggery (C), growth rate was not improved in either piggery and the prevalence of moderate to severe turbinate atrophy was reduced only in piggery C.  相似文献   

Prothrombin time and activated partial thromboplastin time using a point‐of‐care analyser (Abaxis VSpro®) in Bennett's wallabies (Macropus rufogriseus)          下载免费PDF全文

BN Nevitt  SK Chinnadurai  MK Watson  JN Langan  MJ Adkesson 《Australian veterinary journal》2016,94(10):384-386

There are few reports of coagulation times in marsupial species. Blood samples collected from 14 Bennett's wallabies (Macropus rufogriseus) under anaesthesia during routine health assessments were analysed for prothrombin time (PT) and activated partial thromboplastin time (aPTT) using a point‐of‐care analyser (POC) (Abaxis VSPro®). The wallabies had an aPTT mean of 78.09 s and median of 78.1 s. The PT for all wallabies was greater than 35 s, exceeding the longest time measured on the POC. Although PT was significantly longer, aPTT was similar to the manufacturer's domestic canine reference range.  相似文献