Prostatic sarcomatoid carcinoma in a dog: cytologic and immunohistochemical findings     

Pinto da Cunha N  Ghisleni G  Romussi S  Caniatti M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(4):368-372

An 8-year-old neutered male Boxer was presented with tenesmus, hemorrhagic urethral discharge, and dysuria. Abdominal ultrasound and radiographic examinations revealed irregular prostatic enlargement. Laparotomy was performed and intraoperative cytology was done on imprint smears of a biopsy specimen obtained from a prostatic mass. The cytologic preparation was highly cellular and contained a predominant population of atypical, large, loosely cohesive spindle cells, with rare multinucleated cells and mitotic figures. The cytologic findings were consistent with undifferentiated sarcoma. At necropsy, a large cystic prostatic mass and numerous satellite nodules in the soft tissues around the pelvis were found. On histologic examination the tumor was composed primarily of bundles of neoplastic spindle cells. Rare pseudo-acinar structures and signet-ring cells also were observed. On immunohistochemical examination, the neoplastic cells co-expressed cytokeratin and vimentin. Based on histologic and immunohistochemical findings, the tumor was diagnosed as primary prostatic sarcomatoid carcinoma. This is a rare tumor in dogs, in which biphasic morphology of epithelial and mesenchymal cells can complicate the diagnosis, requiring immunochemical stains for confirmation.  相似文献   

Effects of age and season on conception rate of Mediterranean Italian Dairy Buffalo (Bubalus bubalis) following oestrus synchronization and fixed-time artificial insemination     

Gabriel Otava  Stefano Squicciarini  Simona Marc  Tijana Suici  Gary William Onan  Ioan Hutu  Iuliu Torda  Calin Mircu 《Reproduction in domestic animals》2021,56(12):1511-1518

The objective of this project was to report the effects of season and age on conception rate amongst Italian Mediterranean Buffalo subjected to an Ovsynch/Resynch (O/R) reproductive management protocol. The study utilized nulliparous (heifers), primiparous buffalo cows (PBC) and multiparous buffalo cows (MBC). The primiparous and multiparous groups were subjected to the synchronization protocol throughout the entire year, but heifers were synchronized and inseminated only during the spring/summer seasons. The conception rate obtained following the OvSynch oestrus synchronization protocol, applied during spring, was 68.4% for heifers, 83.3% for PBC and 67.7% for MBC. The overall total conception rates following the complete O/R protocol were 84.27%, 94.4% and 79%. Conception rates achieved during summer were heifers 52%, PBC 47.2% and MBC 49%, whilst overall conception rates following the full O/R protocol were 72%, 69.8% and 58.2% respectively. In the autumn seasons, PBC conceived 58.9% and MBC 52.1% following initial Ovsynch, which improved to total overall conception rates of 87.5% and 78.7% following the full O/R protocol. Similarly, in the winter season, PBC experienced a conception rate of 47.5% following Ovsynch and 72.5% after a follow-up Resynch. MBC experienced 60.0% and 74.4% conception following Ovsynch and full O/R, respectively, during winter. Total conception rates during all seasons were quite acceptable following the O/R protocol. There was a significant decrease from spring to summer in conception rate for all parity groups, but heifers were not a severely affected as older buffalo cows. This finding agrees with that of other investigators indicating that heifer fertility is not as negatively impacted by long photoperiod and higher ambient temperature as that of older animals. The O/R protocol as utilized in this study is an effective means of reproductive management for dairy buffalo cows and is effective for improving fertility during out-of-season breeding.  相似文献   

Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. A review     

Youry Pii  Tanja Mimmo  Nicola Tomasi  Roberto Terzano  Stefano Cesco  Carmine Crecchio 《Biology and Fertility of Soils》2015,51(4):403-415

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Book review     

Stefano Peruzzi 《Aquaculture International》2006,14(4):413-414

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Production, nitrogen and carbon balance of maize-based forage systems     

Carlo Grignani  Laura Zavattaro  Dario Sacco  Stefano Monaco 《European Journal of Agronomy》2007,26(4):442-453

Nitrogen (N) and carbon (C) surplus can be used as indicators of an agroecosystems’ ability to maintain soil fertility. Maize is the key crop of intensive forage systems in northern Italy, and large amounts of manure are often supplied to this crop. Different maize-based cropping systems and manure managements were compared in this paper. The following were assessed, using the results of an 11-year experiment: crop production and N uptakes; C and N surpluses; soil C and N contents. The treatments were maize for silage (Ms), maize for grain (Mg), double annual crop rotation maize–Italian ryegrass (Mr), and rotation maize–grass ley (Ml). Five fertilization management systems were adopted: 0N control, and bovine slurry and farmyard manure supplied at two levels, ranging from 215 to 385 kg ha⁻¹ of total N.

The dry-matter production of Mr was significantly higher than those of the other systems. The response of maize to fertilization was similar in all the cropping systems, except for Mr, for which the crop showed a high reactivity to N input at both fertilizer levels. Soil reserves were rapidly consumed in the unfertilized treatment of Mr, whereas the high productivity potential of this cropping system was exerted in fertilized plots. The introduction of a ley in rotation with maize reduced the system's DM production, due to the low yield potential of grass compared to that of maize, reduced the system response to fertilization, and diminished the exploitation of organic N at high fertilization rates. Cumulated N surplus caused an enrichment of the soil N pool size: 43% of excess N was retained by the soil. The relationship between the cumulated C surplus and the soil C pool size indicated that 26–27% was retained by the soil. Crop residues of the Mg system were less effective in building up the soil C pool than other C sources. Both slurry and farmyard manure exerted a positive effect on the soil C and N retention. When farmyard manure was used, 18% of C and 45% of surplus N were incorporated into the soil organic matter (SOM). Slurry also built up the SOM content, resulting in 9% of C and 24% of N surplus.  相似文献   

Analysis of S‐allele genetic diversity in Sicilian almond germplasm comparing different molecular methods          下载免费PDF全文

Sergio Currò  Stefano La Malfa  Gaetano Distefano  Guiyou Long  Francesco Sottile  Alessandra Gentile 《Plant Breeding》2015,134(6):713-718

Italian almond germplasm is characterized by a wide diversity in several growing areas among which Sicily is one of the most important. Analysis with consensus and specific primers and DNA sequencing was performed to investigate S‐RNase genetic diversity and to elucidate the homology rate within a genetic pool of 27 Italian accessions. Interestingly, some of the self‐compatible cultivars did not show the presence of S_f allele. Amplicons from consensus and allele‐specific PCR primers revealed a high level of variability. Sequencing of all the S‐RNase amplicons derived from consensus primers allowed the identification of two new S‐RNase alleles (S₅₁ and S₅₂). Surprisingly, despite the AA replacement mutation, S₅₁ did not exhibit any change of its S‐RNase function. Additionally, several mutations, with no effect on amino acid composition, were detected in the intron and/or in the ORF of four known alleles (S_g, S₁₀, S₃₁ and S₃₅). Genetic variation, regarding point mutations and only detected by sequencing, was revealed among 11 of 27 tested cultivars. The new sources of variability might have an interest for product traceability.  相似文献   

Genetic and environmental factors affecting pathogenicity of wheat as related to celiac disease     

Barbara Prandi  Paola Mantovani  Gianni Galaverna  Stefano Sforza 《Journal of Cereal Science》2014

Gluten proteins are the basis of the rheological properties of wheat derived products, such as bread and pasta. Their particular amino acidic composition (high proline and glutamine content) is responsible for the poor gluten digestibility. Some of the high molecular weight peptides that are generated in the gastrointestinal tract are involved in an autoimmune entheropathy called celiac disease. In this work we compared the amount of peptides containing sequences involved in adaptive and immune responses, which were produced after simulated gastrointestinal digestion of prolamins extracted from different durum wheat varieties and in-bred lines. Peptides containing sequences involved in celiac disease were quantified using an isotopically labeled peptide as internal standard. The results demonstrated a very high variability in the amount of pathogenic peptides produced by different lines, showing a strong contribution of the genetic component. At the same time, the variability in total protein and gluten content was lower; the weak correlation between pathogenic peptides and the amount of gluten proteins gives rise to the possibility of a varietal selection aimed to maintain good rheological properties, but simultaneously reducing the exposure to peptides eliciting an immunological response in celiac predisposed subjects. These varieties might be useful for celiac disease prevention.  相似文献   

Phallusiasterols A and B: Two New Sulfated Sterols from the Mediterranean Tunicate Phallusia fumigata and Their Effects as Modulators of the PXR Receptor     

Concetta Imperatore  Filomena D’Aniello  Anna Aiello  Stefano Fiorucci  Claudio D’Amore  Valentina Sepe  Marialuisa Menna 《Marine drugs》2014,12(4):2066-2078

Purification of the apolar extracts of the marine ascidian Phallusia fumigata, afforded two new sulfated sterols, phallusiasterols A (1) and B (2). The structures of the new compounds have been elucidated using mass spectrometry and NMR experiments. The effects of phallusiasterols A and B as modulators of pregnane-X-receptor (PXR) have been investigated. These studies revealed that phallusiasterol A induces PXR transactivation in HepG2 cells and stimulates the expression of the PXR target genes CYP3A4 and MDR1 in the same cell line. Molecular docking calculations suggested the theoretical binding mode of phallusiasterol A with hPXR and revealed that phallusiasterol A fitted well in the LBD of PXR.  相似文献   

Marine and Semi-Synthetic Hydroxysteroids as New Scaffolds for Pregnane X Receptor Modulation     

Valentina Sepe  Francesco Saverio Di Leva  Claudio D’Amore  Carmen Festa  Simona De Marino  Barbara Renga  Maria Valeria D’Auria  Ettore Novellino  Vittorio Limongelli  Lisette D’Souza  Mahesh Majik  Angela Zampella  Stefano Fiorucci 《Marine drugs》2014,12(6):3091-3115

In recent years many sterols with unusual structures and promising biological profiles have been identified from marine sources. Here we report the isolation of a series of 24-alkylated-hydroxysteroids from the soft coral Sinularia kavarattiensis, acting as pregnane X receptor (PXR) modulators. Starting from this scaffold a number of derivatives were prepared and evaluated for their ability to activate the PXR by assessing transactivation and quantifying gene expression. Our study reveals that ergost-5-en-3β-ol (4) induces PXR transactivation in HepG2 cells and stimulates the expression of the PXR target gene CYP3A4. To shed light on the molecular basis of the interaction between these ligands and PXR, we investigated, through docking simulations, the binding mechanism of the most potent compound of the series, 4, to the PXR. Our findings provide useful functional and structural information to guide further investigations and drug design.  相似文献   

Review on iron availability in soil: interaction of Fe minerals,plants, and microbes   总被引：1，自引：0，他引：1  

Claudio Colombo  Giuseppe Palumbo  Ji-Zheng He  Roberto Pinton  Stefano Cesco 《Journal of Soils and Sediments》2014,14(3):538-548

Purpose

The rationale of this paper is to review the state of the art regarding the biotic and abiotic reactions that can influence Fe availability in soils. In soil, the management-induced change from oxic to anoxic environment results in temporal and spatial variations of redox reactions, which, in turn, affect the Fe dynamics and Fe mineral constituents. Measuring the Fe forms in organic complexes and the interaction between bacteria and Fe is a major challenge in getting a better quantitative understanding of the dynamics of Fe in complex soil ecosystems.

Materials and methods

We review the existing literature on chemical and biochemical processes in soils related with the availability of Fe that influences plant nutrition. We describe Fe acquisition by plant and bacteria, and the different Fe–organic complexes in order to understand their relationships and the role of Fe in the soil carbon cycle.

Results and discussion

Although total Fe is generally high in soil, the magnitude of its available fraction is generally very low and is governed by very low solubility of Fe oxides. Plants and microorganisms can have different strategies in order to improve Fe uptake including the release of organic molecules and metabolites able to form complexes with Fe^III. Microorganisms appear to be highly competitive for Fe compared with plant roots. Crystalline Fe and poorly crystalline (hydro)oxides are also able to influence the carbon storage in soil.

Conclusion

The solubility of crystalline Fe minerals in soil is usually very low; however, the interaction with plant, microbes, and organic substance can improve the formation of soluble Fe^III complexes and increase the availability of Fe for plant growth. Microbes release siderophores and plant exudates (e.g., phytosiderophores, organic acids, and flavonoids), which can bind and solubilize the Fe present in minerals. The improved understanding of this topic can enable the identification of effective solutions for remedying Fe deficiency or, alternatively, restricting the onset of its symptoms and yield’s limitations in crops. Therefore, development and testing of new analytical techniques and an integrated approach between soil biology and soil chemistry are important prerequisites.  相似文献