Estimated genetic parameters for palatability traits of steaks from Brahman cattle     

Riley DG  Chase CC  Hammond AC  West RL  Johnson DD  Olson TA  Coleman SW 《Journal of animal science》2003,81(1):54-60

Heritabilities and genetic and phenotypic correlations were estimated from carcass and beef palatability data collected from Brahman calves (n = 504) born in central Florida from 1996 to 2000. Traits evaluated included Warner-Bratzler shear force (after 7, 14, and 21 d of aging), panel tenderness score, connective tissue amount, juiciness, flavor intensity, and off flavor (after 14 d of aging), percentages of raw and cooked lipids, and milligrams per gram of muscle calpastatin activity. Parameters were estimated using an animal model and derivative-free restricted maximum likelihood procedures. Estimated heritabilities for d 7, 14, and 21 shear force were 0.14,0.14, and 0.06, respectively, indicating that improvement in these traits by selection would be slow. Estimated heritabilities of sensory panel attributes were 0.11, 0.12, 0.05, 0.04, and 0.01 for tenderness, connective tissue amount, juiciness, flavor intensity, and off flavor, respectively. The estimated heritabilities for percentages of raw and cooked lipids, and calpastatin activity were 0.34, 0.17, and 0.07, respectively. Most of the estimated genetic correlations among palatability traits and for palatability traits with fat thickness, marbling score, and loin muscle area were consistent with other estimates from the literature. Results indicated that improvement in tenderness based on selection for favorable shear force, sensory panel tenderness, or calpastatin activity would be slow; therefore, postslaughter intervention programs should also be considered.  相似文献   

Sodium nitroprusside-induced deliberate hypotension to facilitate patent ductus arteriosus ligation in dogs     

Hunter SL  Culp LB  Muir WW  Lerche P  Birchard SJ  Smeak DD  McLoughlin MA 《Veterinary surgery : VS》2003,32(4):336-340

OBJECTIVE: To report the use of sodium nitroprusside to induce deliberate hypotension to reduce hemorrhage and facilitate surgical ligation of complicated patent ductus arteriosus (PDA) in dogs. STUDY DESIGN: Retrospective study. ANIMALS: Six dogs. METHODS: Hemorrhage occurred during surgical ligation of PDA in 5 dogs. Surgical dissection and isolation of the PDA were very difficult in a sixth dog that was considered to be at increased risk for ductus rupture. Sodium nitroprusside (5 to 25 microg/kg/min intravenously) was administered to these 6 dogs to induce hypotension to reduce blood loss and facilitate surgical ligation of the PDA. RESULTS: Sodium nitroprusside infusion reduced blood pressure (mean arterial pressure, 45 to 60 mm Hg) within 5 to 10 minutes and hemorrhage from the PDA, facilitating its surgical ligation. Recovery from surgery and anesthesia was uneventful in all 6 dogs. CONCLUSIONS: Sodium nitroprusside infusion can be used to induce deliberate hypotension in dogs to facilitate surgical ligation of PDA. CLINICAL RELEVANCE: Sodium nitroprusside infusion produces hypotension within 5 to 10 minutes and is easy to control, rapidly abates, and should help to facilitate surgical ligation of PDA.  相似文献   

A recombinant vaccine expressing a mammalian Mycobacterium sp. antigen is immunostimulatory but not protective in striped bass     

Pasnik DJ  Vemulapalli R  Smith SA  Schurig GG 《Veterinary immunology and immunopathology》2003,95(1-2):43-52

A recombinant vaccine was constructed for piscine mycobacteriosis utilizing a Brucella abortus strain RB51 vector expressing a mammalian Mycobacterium sp. 85A antigen. Juvenile striped bass were inoculated with the resulting construct at doses equivalent to 10(6), 10(7), 10(8), 10(9), and 10(10) colony-forming units/fish. Blood and tissue samples from these fish demonstrated significant specific humoral and cell-mediated immune responses towards the 85A antigen in a dose-dependent manner. However, survival studies determined that inoculated fish failed to demonstrate cross-protective responses after live Mycobacterium marinum challenge 70 days post-inoculation.  相似文献   

Prevalence of cpb2, encoding beta2 toxin,in Clostridium perfringens field isolates: correlation of genotype with phenotype   总被引：4，自引：0，他引：4  

Bueschel DM  Jost BH  Billington SJ  Trinh HT  Songer JG 《Veterinary microbiology》2003,94(2):121-129

Beta2 toxin, encoded by the cpb2 gene, has been implicated in the pathogenesis of porcine, equine and bovine enteritis by type A Clostridium perfringens. By incorporating primers to cpb2 into a multiplex genotyping PCR, we screened 3270 field isolates of C. perfringens. Of these, 37.2% were PCR positive for the cpb2 gene. The majority of isolates from cases of porcine enteritis were positive for cpb2 (>85%), and this was even more true for C. perfringens isolated from cases of porcine neonatal enteritis (91.8%). In contrast, isolates from normal pigs only contained cpb2 in 11.1% of cases. The correlation between enteritis in other animal species and the presence of cpb2 was not so strong. cpb2 was found in 21.4% of C. perfringens isolates from cattle with enteritis, and in 47.3% of isolates from calves with enteritis or abomastitis. The prevalence of cpb2 varied with genotype, with type A isolates being positive for this gene in 35.1% of cases. Furthermore, enterotoxigenic type D or type E strains almost always carried cpb2. We cloned a 6xHIS-tagged beta2 (HIS-beta2) and used this protein to raise antiserum against beta2. Culture supernatants from 68 cpb2-positive and 13 cpb2-negative strains were tested for the presence of beta2 by Western blotting. In cpb2-positive isolates of porcine origin, beta2 was almost always detected (96.9%). However, in cpb2-positive isolates from other animal species, only 50.0% expressed beta2 protein. The high rate of cpb2-positivity among strains from neonatal pigs with enteritis and the high correlation of genotype with phenotype, supports the contention that beta2 toxin plays a role in the pathogenesis of these infections. However, it may be important to consider the use of an additional method for the detection of beta2 toxin in non-porcine cpb2-positive isolates when making claims about the role of beta2 in enteritis in non-porcine species.  相似文献   

Combined dorsolateral and intraoral approach for the resection of tumors of the maxilla in the dog     

Lascelles BD  Thomson MJ  Dernell WS  Straw RC  Lafferty M  Withrow SJ 《Journal of the American Animal Hospital Association》2003,39(3):294-305

This paper describes in detail a combined dorsal and intraoral approach for maxillectomy for tumors involving tissues more caudal to the third premolar. The only intraoperative complication was that of blood loss, with six out of 20 dogs requiring a single unit of blood. Histopathologically clean margins were obtained in 14 of the 20 cases, with a recurrence rate of 50% in these dogs and a median time to recurrence of 24 months. This represents an improvement in outcome over previously reported studies, and the authors postulate this is due to the better exposure and access to the area afforded by the combined approach over the standard intraoral approach.  相似文献   

Not classical homeopathy          下载免费PDF全文

Drosdovech M  Neumann S  Evans D  Blake S  Peck LS  Yearout DR  Ulbrich B  Facinelli J  Delgado R  Hoberg L  Harman J  Sthamann M  Lindsay M  Kwong H  Lampru A  Lamb J  Stagmeier K  Doyle JL  Elliot J  Siemon L 《The Canadian veterinary journal. La revue veterinaire canadienne》2002,43(12):908-909

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Rapid communication: Thirty-eight polymorphic microsatellite markers for mapping in rainbow trout   总被引：8，自引：0，他引：8  

Rexroad CE  Coleman RL  Hershberger WK  Killefer J 《Journal of animal science》2002,80(2):541-542

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Efficacy of extended pirlimycin hydrochloride therapy for treatment of environmental Streptococcus spp and Staphylococcus aureus intramammary infections in lactating dairy cows   总被引：1，自引：0，他引：1  

Gillespie BE  Moorehead H  Lunn P  Dowlen HH  Johnson DL  Lamar KC  Lewis MJ  Ivey SJ  Hallberg JW  Chester ST  Oliver SP 《Veterinary therapeutics : research in applied veterinary medicine》2002,3(4):373-380

Fifty-one chronically infected lactating dairy cows were used to evaluate the efficacy of extended pirlimycin therapy regimens for treatment of intramammary infections by environmental Streptococcus spp and Staphylococcus aureus. Cows (n = 47) with one or more infected mammary quarters were blocked by parity and randomly allocated to one of three groups for treatment with pirlimycin (50 mg/mammary quarter) as follows: one treatment per day for 2 days (n = 36 infected mammary quarters); one treatment per day for 5 days (n = 36 infected mammary quarters); and one treatment per day for 8 days (n = 20 infected mammary quarters). Four cows with nine infected mammary quarters were included as untreated controls. Milk samples from each mammary quarter were collected 7 days before treatment, immediately before treatment, and weekly for 4 weeks after the final treatment for microbiological evaluation. A bacteriologic cure was defined as a treated, infected quarter that was bacteriologically negative for the presence of previously identified bacteria at weekly intervals after treatment. Efficacy of pirlimycin therapy against intramammary infections caused by environmental Streptococcus spp and S. aureus was 44.4%, 61.1%, and 95.0% for the 2-, 5-, and 8-day treatment regimens, respectively. None of the infections in the untreated control quarters was cured. Significant differences in efficacy were detected between all pirlimycin groups and the untreated control group, between the 8- and 2-day treatment regimens, and between the 8-day and 5-day treatment regimens (P < or = .05). Results of this study indicate that extended pirlimycin therapy was effective in eliminating intramammary infections caused by environmental streptococci and S. aureus in lactating dairy cows.  相似文献   

Antihistamines in the management of canine atopic dermatitis: a retrospective study of 171 dogs (1992-1998)     

Zur G  Ihrke PJ  White SD  Kass PH 《Veterinary therapeutics : research in applied veterinary medicine》2002,3(1):88-96

Antihistamines were prescribed for 178 of 271 dogs with a diagnosis of atopic dermatitis at the Veterinary Medical Teaching Hospital of the University of California, Davis from 1992 to 1998. Fifty-four percent of 166 dogs given antihistamines responded favorably to these treatments, with 27% of the responses rated as good and 27% rated as moderate. Diphenhydramine and hydroxyzine were the most commonly used antihistamines and were the most frequently effective. Chlorpheniramine and clemastine were administered less frequently and had much lower positive response rates. Responses to antihistamines as a group were significantly better in dogs having onset of clinical signs at younger ages (odds ratios for 1-year increase in age = 0.72, 95% confidence interval = 0.57 to 0.91, P =.005).  相似文献   

Prevalence and diversity of Arcobacter spp. isolated from the internal organs of spontaneous porcine abortions in Denmark     

On SL  Jensen TK  Bille-Hansen V  Jorsal SE  Vandamme P 《Veterinary microbiology》2002,85(2):159-167

A study was conducted to determine the prevalence and possible significance of campylobacteria in pig abortions in Denmark. Surface-cauterised liver and kidney samples from 55 aborted pig fetuses submitted to the Danish Veterinary Laboratory were taken and a sensitive isolation procedure used to examine pooled tissue samples for Campylobacter, Arcobacter and Helicobacter spp. Routine microbiological, immunological, and histopathological examinations were also performed to identify concurrent infections or histopathological changes. The abortions tested negative for established abortifacient pathogens (Brucella, Leptospira, PPV, PRRSV), but Arcobacter spp. were recovered from 23/55 abortions. Co-infections with Streptococcus suis, Escherichia coli, and haemolytic streptococci were observed in 7/23 Arcobacter-positive fetuses, and in 4/32 Arcobacter-negative fetuses. Histopathological analyses identified placentitis, pneumonia, hepatitis and encephalitis among the study group. However, no obvious pathologic features were solely associated with Arcobacter-positive cases, nor were Arcobacter-like bacteria observed in tissue samples. Protein profile analyses of the 27 Arcobacter isolates identified 11 as A. cryaerophilus and 10 as A. skirrowii. Six strains could not be classified into any existing species and were phenotypically distinct, thus, potentially representing at least one new species. The identification results showed that multiple taxa could be found in a single fetus, and in distinct aborted fetuses from a single sow. The high prevalence of arcobacters in Danish pig abortions may account for at least some of the >90% of cases in which no established abortifacient agent is detected, but further studies are needed to define the role of each species, especially where co-infections with other bacteria are present.  相似文献