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91.
Larson JE Thielen KN Funnell BJ Stevenson JS Kesler DJ Lamb GC 《Journal of animal science》2009,87(3):914-921
We determined whether an ovulatory estrus could be resynchronized in previously synchronized, AI nonpregnant cows without compromising pregnancy from the previous synchronized ovulation or to those inseminated at the resynchronized estrus. Ovulation was synchronized in 937 suckled beef cows at 6 locations using a CO-Synch + progesterone insert (controlled internal drug release; CIDR) protocol [a 100-microg injection of GnRH at the time of progesterone insert, followed in 7 d by a 25-mg injection of PGF(2alpha) at insert removal; at 60 h after PGF(2alpha), cows received a fixed-time AI (TAI) plus a second injection of GnRH]. After initial TAI, the cows were assigned randomly to 1 of 4 treatments: 1) untreated (control; n = 237); 2) progesterone insert at 5 d after TAI and removed 14 d after TAI (CIDR5-14; n = 234); 3) progesterone insert placed at 14 d after TAI and removed 21 d after TAI (CIDR14-21; n = 232); or 4) progesterone insert at 5 d after TAI and removed 14 d after TAI and then a new CIDR inserted at 14 d and removed 21 d after TAI (CIDR5-21; n = 234). After TAI, cows were observed twice daily until 25 d after TAI for estrus and inseminated according to the AM-PM rule. Pregnancy was determined at 30 and 60 d after TAI to determine conception to the first and second AI. Pregnancy rates to TAI were similar for control (55%), CIDR5-14 (53%), CIDR14-21 (48%), and CIDR5-21 (53%). A greater (P < 0.05) proportion of nonpregnant cows was detected in estrus in the CIDR5-21 (76/110, 69%) and CIDR14-21 (77/120, 64%) treatments than in controls (44/106, 42%) and CIDR5-14 (39/109, 36%) cows. Although overall pregnancy rates after second AI service were similar, combined conception rates of treatments without a CIDR from d 14 to 21 [68.7% (57/83); control and CIDR5-14 treatments] were greater (P = 0.03) than those with a CIDR during that same interval [53.5% (82/153); CIDR5-21 and CIDR14-21 treatments]. We conclude that placement of a progesterone insert 5 d after a TAI did not compromise or enhance pregnancy rates to TAI; however, conception rates of nonpregnant cows inseminated after a detected estrus were compromised when resynchronized with a CIDR from d 5 or 14 until 21 d after TAI. 相似文献
92.
Broderick G A Stevenson M J Patton R A 《饲料博览》2009,(7):43-44
选用32头经产和16头初产荷斯坦奶牛进行不完全8×8拉丁方设计试验(4周为期,共12周),进行评估粗蛋白(CP)、可消化瘤胃非降解蛋白(RUP)和瘤胃保护蛋氨酸(RPM,饲喂的是美国乔治亚州肯尼索德固赛公司的产品Mepron)。日粮舍(按干物质(DM)计)苜蓿青贮21%、玉米青贮34%、高水分玉米22%-26%、豆粕10%-14%、大豆皮4%、添加脂肪2%、矿物质和维生素13%、中性洗涤纤维27%-28%。添加或不添加压榨大豆粕的瘤胃降解蛋白(RUP),配制为15.8%或17.1%日粮粗蛋白,0或9g RPM/d的2×2×2因子设计。结果表明,二向或三向交互作用都不显著。高日粮CP增加表明采食量1.1kg·d^-1 DM、产奶量1.7kg·d^-1、3.5%乳脂校正乳(FCM)2.2kg·d^-1、乳脂量0.10kg·d^-1、真蛋白0.05kg·d^-1,改善了表观氮平衡及干物质与纤维的消化率。然而,母牛采食较高日粮CP时,乳中尿素氮和估计尿液尿素氮和总氮排泄也增加,表观氮效率(牛奶中氮量,采食氮量)从33%下降到30%。饲喂较多RUP的积极效果在于提高饲料转化率和乳脂肪含量(FCM增加1.8kg·d^-1,乳脂量增加0.08kg·d^-1),但是乳蛋白含量下降,乳中尿素氮和尿液尿素排泄增加..添加RPM增加DM采食量0.7kg·d^-1,FCM增加1.4kg·d^-1,乳脂量增加0.06kg·d^-1,并有提高乳脂率、产奶量和乳蛋白产量的趋势。 相似文献
93.
CEDAR M. CHITTENDEN BJØRN ÅDLANDSVIK OLE‐PETTER PEDERSEN DAVID RIGHTON AUDUN H. RIKARDSEN 《Fisheries Oceanography》2013,22(1):1-13
The use of pop‐up archival satellite tags (PSATs) to geolocate marine fishes in polar regions is challenging due to the brevity of periods during which there is a defined sunrise and sunset. Models using other environmental parameters are thus required to supplement geolocation data in the estimation of marine migratory routes. The objective of this work was to create a simple method that would estimate the migratory pathways of Atlantic salmon (Salmo salar) in polar regions using temperature and depth recordings. Validated geolocations from PSATs were used to test and constrict the model. The model’s predicted migratory routes were within 100 km of the light‐based geolocations calculated by the tags. By constraining the trajectories through the geolocations, bias was reduced. Sensitivity analyses demonstrated that slight alterations of the location and timing of the start and end points did not affect the mean migratory route estimates. This method is a management tool that can determine the primary habitat areas for any surface‐ or bottom‐dwelling marine species – especially in polar regions, where other methods may be impossible. 相似文献
94.
The national control programme for Salmonella in Danish swine herds introduced in 1993 has led to a large decrease in pork-associated human cases of salmonellosis. The pork industry is increasingly focused on the cost-effectiveness of surveillance while maintaining consumer confidence in the pork food supply. Using national control programme data from 2003 and 2004, we developed a zero-inflated binomial model to predict which farms were most at risk of Salmonella. We preferentially sampled these high-risk farms using two sampling schemes based on model predictions resulting from a farm's covariate pattern and its random effect. Zero-inflated binomial modelling allows assessment of similarities and differences between factors that affect herd infection status (introduction), and those that affect the seroprevalence in infected herds (persistence and spread). Both large (producing greater than 5000 pigs per annum), and small herds (producing less than 2000 pigs per annum) were at significantly higher risk for infection and subsequent seroprevalence, when compared with medium sized herds (producing between 2000 and 5000 pigs per annum). When compared with herds being located elsewhere, being located in the south of Jutland significantly decreased the risk of herd infection, but increased the risk of a pig from an infected herd being seropositive. The model suggested that many of the herds where Salmonella was not detected were infected, but at a low prevalence. Using cost and sensitivity, we compared the results of our model based sampling schemes with those under the standard sampling scheme, based on herd size, and the recently introduced risk-based approach. Model-based results were less sensitive but show significant cost savings. Further model refinements, sampling schemes and the methods to evaluate their performance are important areas for future work, and these should continue to occur in direct consultation with Danish authorities. 相似文献
95.
Objective To evaluate the effect of the number of days exposure to a prepartum transition diet on mineral, energy and protein metabolism in dairy cows. Design A prospective cohort study. Procedures The prepartum transition diet consisted of ryegrass pasture, cereal hay, grain, grain by-product, protein meals, BioChlor®, rumen modifiers, minerals and vitamins and contained 13.9% crude protein, 10.0 MJ metabolisable energy/kg and a dietary cation anion difference of −35 meq/kg dry matter. Forty cows were bled bi-weekly from their introduction to the prepartum transition diet until day 35 of lactation. Blood samples were submitted for estimation of a range of metabolites. Cubic smoothed splines were fitted to scatterplots of metabolite concentration as a function of day relative to calving and the area under the curve (AUC) was calculated. Linear regression modelling determined the effect of days exposed, age, body condition score (BSC) and calving day on AUC. Results The prepartum AUC of blood phosphorus and beta-hydroxybutyrate increased and the AUC of blood calcium and cholesterol decreased with increasing days exposed to the diet. The postpartum AUC of beta-hydroxybutyrate and non-esterified fatty acids decreased with increasing days exposed but increased with days exposed × age and days exposed × BCS, respectively. The AUC of other metabolites did not vary significantly with number of days exposed. Conclusions Increasing exposure to the prepartum diet significantly altered the AUC of blood metabolites associated with mineral, energy and protein metabolism in ways consistent with improvements in production and reproduction previously reported and the result suggests links between energy, protein and skeletal metabolism. 相似文献
96.
E. TYDÉN H. BJŐRNSTRŐM H. TJÄLVE P. LARSSON 《Journal of veterinary pharmacology and therapeutics》2010,33(4):332-340
Tydén, E., Bj?rnstr?m, H., Tjälve, H., Larsson, P. Expression and localization of BCRP, MRP1 and MRP2 in intestines, liver and kidney in horse. J. vet. Pharmacol. Therap. doi: 10.1111/j.1365‐2885.2009.01140.x. The gene and protein expression and the cellular localization of the ABC transport proteins breast cancer resistance protein (BCRP), multidrug resistance‐associated protein 1 (MRP1) and multidrug resistance‐associated protein 2 (MRP2) have been examined in the intestines, liver and kidney in horse. High gene and protein expression of BCRP and MRP2 were found in the small intestines, with cellular localization in the apical membranes of the enterocytes. In the liver, MRP2 was present in the bile canalicular membranes of the hepatocytes, whereas BCRP was localized in the cytoplasm of hepatocytes in the peripheral parts of the liver lobuli. In the kidney both BCRP and MRP2 were predominantly present in the distal tubuli and in the loops of Henle. In most tissues, the gene and protein expression of MRP1 were much lower than for BCRP and MRP2. Immunostaining of MRP1 was detectable only in the intestines and with localization in the cytoplasm of enterocytes in the caecum and colon and in the cells of serous acini of Brunner’s glands in the duodenum and the upper jejunum. The latter cells were also stained for BCRP, but not for MRP2. Many drugs used in horse are substrates for one or more of the ABC transport proteins. These transporters may therefore have important functions for oral bioavailability, distribution and excretion of substrate compounds in horse. 相似文献
97.
P.W. Greig-Smith H.M. Thompson A.R. Hardy M.H. Bew E. Findlay J.H. Stevenson 《Crop Protection》1994,13(8):567-581
Suspected cases of poisoning of honeybees by agricultural pesticides are investigated by the Ministry of Agriculture, Fisheries and Food (in England and Wales) and the Scottish Office Agriculture and Fisheries Department (in Scotland). Studies include field visits to sites where bee mortality is recorded, examination of dead bees for disease, and analysis for the presence of pesticide residues. This paper reviews the results of these investigations for the period 1981–1991. Pesticide poisoning is diagnosed from the detection of residues in dead bees, through interpretation in relation to the median lethal dose (LD50), and ‘subsequent residue level’ (SRL) determined from laboratory studies in which bees were exposed to one LD50 dose. Overall, an annual average of 50 incidents were confirmed as due to pesticide poisoning, involving 30 pesticide active ingredients during the 1981–1991 period. In England and Wales, the principal hazards were caused by misuse of the insecticide triazophos on oilseed rape, a problem that has been reduced to low levels during the 1980s, and by dimethoate use on a variety of arable crops. In Scotland there were fewer incidents, most of which were associated with use of fenitrothion on raspberries, and gamma-HCH on oilseed rape. Opportunities for reducing the risks of poisoning, and for refining the investigation of suspected incidents, are discussed. 相似文献
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